research centers


Search results: Found 13

Listing 1 - 10 of 13 << page
of 2
>>
Sort by

Article
Immunoglobulines and complements levels in sera of patients with thalassemia

Author: Wafaa Sadoon Shani
Journal: Journal of University of Babylon مجلة جامعة بابل ISSN: 19920652 23128135 Year: 2014 Volume: 22 Issue: 9 Pages: 2507-2503
Publisher: Babylon University جامعة بابل

Loading...
Loading...
Abstract

This study designed to analyzed humoral response related to measuring the serum levels of immunoglobulines (IgG , IgM and IgA) and complements corporeats ( C3 and C4) in (20) thalassemic patient and (10) control subject. IgG only recorded a high significant differences between two studied group – whereas no significant differences were found in levels of remaining humoral components ( IgM , IgA , C3 and C4 ) between thalassemic patients and control group .

صممت هذه الدراسة لتحليل الاستجابة المناعية الخلطية المتعلقة بقياس مستويات الكلوبيولينات المناعية ( IgG , IgM , IgA ) وكذلك مكونات المتمم (C3 , C4 ) في مصول (20) من المرضى المصابين بالثالاسيميا و (10) من السيطرة . وقد لوحظ بان مستويات IgG فقط سجلت فروقات معنوية عالية مجموعتي الدراسة . بينما لم يسجل أي فارق معنوي في مستويات بقية المكونات الخلطية ( IgG , IgM , C3 , C4 ) بين مرضى الثالاسيميا و السيطرة .


Article
Seroepidemiological Screening For Toxoplasmosis Within Unmarried Women In Basrah Province
التحري المصلي الوبائي عن داء المقوسات ضمن النساء غير المتزوجات في محافظة البصرة

Authors: Wafaa Sadoon Shani --- Ghalib Noori AL-Mousawi
Journal: JOURNAL OF THI-QAR SCIENCE مجلة علوم ذي قار ISSN: 19918690 Year: 2011 Volume: 2 Issue: 4 Pages: 34-40
Publisher: Thi-Qar University جامعة ذي قار

Loading...
Loading...
Abstract

Current study included Seroepidemiological Screening of 450 sera from unmarried women, within age ranged between (15 – 45) year by using IgG and IgM Enzyme Linked Immunosorbent Assay (ELISA) test. Sera collected from areas of Basrah province and from different classes ( students , officials and housewives ) , and according to the primary health care department program the province was divided into eight sections .
Results documented that the prevalence rate of the disease in unmarred women in the whole province was 58% while the prevalence rates of sections was as follows : Sec I 63.2% , Sec II 51.8% , Al – Midayna 75% , AI – Qurna 64% , Al – Hartha 70% , Shatt – al – Arab 55% , Abu – al – Khaseeb 67.5% and Al – Zubair 36.3% . The higher rate recorded in Al – Midayna and lower rate in Al – Zubair .
According to age group there is a significant difference ( P<0.025 ) and the results showed that (15 - 19) year with the rate of infection 64.54% , (30-34) year with rate 60.7% , (35-39) year with rate 60%, while the results upon sociodemographic data recorded that the housewives (all of them with low level of learning) represents rate 62.4% in comparison with students 45% and officials 44% , with a significant differences ( P < 0.05 ) .

Keywords


Article
LEVELS OF SERUM IMMUNOGLOBULINS AND COMPLEMENTS IN PATIENTS WITH VISCERAL LEISHMANIASIS
مستويات الكلوبلينات المناعية ومكونات المتمم في مصول المرضى المصابين باللشمانبا الاحشائية

Author: Wafaa Sadoon Shani وفاء سعدون شاني
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: Print:18138497 E; 24108456 Year: 2006 Volume: 5 Issue: 2 Pages: 6-11
Publisher: Basrah University جامعة البصرة

Loading...
Loading...
Abstract

Forty sera of patients infected with Leishmania donovani and (10) sera from apparentlyhealthy subject were tested by single radial immunodiffusion to visualize the concentration of IgG,IgM, IgA, C3 and C4. Recent results showed that there was a highly significant increase in the levelof IgG, IgM, C3 and C4 but there were no significant differences in the level of IgA between thetwo—studied groups.

Keywords

IgG --- leishmaniasis --- Heteroxenous.


Article
The Effect of Exercise on Total blood count, Phagocytic Activity and Immunoglobulin's levels
تأثير التمرين على تعداد الدم الكلي و الفعالية البلعمية و الكلوبيولينات المناعية

Author: Wafaa Sadoon Shani وفاء سعدون شاني
Journal: Al-Qadisiyah Journal of pure science(quarterly) مجلة القادسية للعلوم الصرفة (فصلية). ISSN: 19972490 Year: 2012 Volume: 17 Issue: 3 Pages: 8-16
Publisher: Al-Qadisiyah University جامعة القادسية

Loading...
Loading...
Abstract

Present work included the study of cellular and humoral immune response in athletes before and after running on treadmill. Results of cellular immune response related with differential white blood cell count indicated an increasing in mean number of neutrophils after exercise in comparison with their number before exercise but it is not significant, whearas the eosinophils and basophils did not show any significant differences in their numbers between the two intervals of study (before and after exercise). Also there is a significant elevation in numbers of monocytes after an exercise in comparison with athletes before an exercise and control group. The study also indicated that there is non significant increasing in lymphocytes after an exercise when compared with their number before an exercise. Moreover results of total white blood cell count indicated that there is an increasing in their numbers in athletes after an exercise. Other indicator of cellular immune response is the presence of an elevation in phagocytic activity of PMNs after an exercise in comparison with their activity before an exercise. In relation with results of humoral immune response, present results demonstrated the significant increasing in IgM and IgA in athletes after an exercise while IgG donot revealed any significant differences in their level among the studied groups.

شمل العمل الحالي دراسة الاستجابة المناعية الخلوية والخلطية في الرياضيين قبل وبعد الركض على شريط الجهد. وقد أكدت نتائج الاستجابة المناعية الخلوية والمتعلقة بحساب العد التفريقي لكريات الدم البيضاء حصول زيادة في معدل أعداد الخلايا العدلة بعد أداء التمرين مقارنة مع أعدادها قبل التمرين لكنها غير معنوية. أما بالنسبة للخلايا الحمضة والقعدة فلم يظهر أي فارق معنوي في أعدادها بين فترتي الدراسة (قبل وبعد أداء التمرين) لكن لوحظ ارتفاعاً معنوياً في أعداد الخلايا الوحيدة النواة بعد أداء التمرين مقارنة مع الرياضيين قبل أداء التمرين والسيطرة. كما أكدت الدراسة حدوث زيادة في الخلايا اللمفية بعد أداء التمرين لكنها غير معنوية مقارنة بأعدادها قبل أداء التمرين. فضلاً عن ذلك فقد أوضحت نتائج العد الكلي لكريات الدم البيض حدوث زيادة فيه في الرياضيين بعد أداء التمرين. ومن مؤشرات الاستجابة المناعية الخلوية هو حصول زيادة في القابلية البلعمية للخلايا المتعددة الانوية بعد أداء التمرين مقارنة بفعاليتها بعد أداء التمرين. وفيما يتعلق بنتائج الاستجابة المناعية الخلطية فقد أوضحت النتائج حدوث زيادة معنوية في IgM وIgA في الرياضيين بعد أداء التمرين, اما IgG فلم تشير النتائج الى اي فارق معنوي في قيمته بين المجاميع المدروسة.

Keywords


Article
Detection of Phagocytic Activity and T , B and T – Helper lymphocytes in peripheral Blood of Women Infected with Toxoplasmosis in Basrah Province
تحديد الفعالية البلعمية و الخلايا اللمفاوية التائية و البائية و الخلايا المساعدة في الدم المحيطي للنساء المصابات بداء المقوسات في محافظة البصرة

Author: Wafaa Sadoon Shani وفاء سعدون شاني
Journal: Al-Qadisiyah Journal of pure science(quarterly) مجلة القادسية للعلوم الصرفة (فصلية). ISSN: 19972490 Year: 2010 Volume: 15 Issue: 1 Pages: 18-25
Publisher: Al-Qadisiyah University جامعة القادسية

Loading...
Loading...
Abstract

A luminol – enhanced chemiluminescence assay was performed to detect the phagocytic activity of polymorph nuclear cells (PMN) in peripheral blood of women infected with Toxoplasma gondii . The phagocytic activity of whole blood cells showed high significant differences between the peaks of both patients and control group . In addition immunophentyping analysis was done by using CD3 , CD20 and CD4+ monoclonal antibodies to estimate the mean percentage of T , B and T – helper lymphocytes in peripheral blood of women with toxoplasmosis . Our results indicated that there was no significant differences in mean percentage of T , B lymphocytes between patients and control group . Null cells numbers also did not show any significant differences between the previous studied groups , while the mean percentage of T – helper lymphocytes subset demonstrated a high significant decrease in patients in comparison with control group .

لقد تم حساب الفعالية البلعمية باستخدام فحص الاستضاءة الكيميائية المسهل باللومينول للخلايا متعددة الانوية في الدم المحيطي للنساء المصابات بـ Toxoplasma gondii و قد اظهرت الفعالية البلعمية لخلايا الدم اختلافات معنوية عالية بين المرضى و مجموعة السيطرة ، فضلاً عن ذلك أجريت الطرز المظهرية المناعية باستخدام الاضداد الاحادية النسيلة CD4+ , CD20 , CD3 لحساب معدل النسبة المئوية للخلايا التائية و البائية و التائية المساعدة في الدم المحيطي للنساء المصابات بداء المقوسات و قد اكدت النتائج الحالية انعدام الفروق المعنوية في معدل النسبة المئوية للخلايا اللمفاوية التائية و البائية بين المرضى و مجموعة السيطرة .و لم تظهر أعداد خلايا Null أي فروقات معنوية بين المجاميع المدروسة السابقة . بينما اوضح معدل النسبة المئوية للخلايا التائية المساعدة وجود نقص معنوي عالي في المرضى بالمقارنة مع مجموعة السيطرة.

Keywords


Article
The association of IL-10 (-592A/C) gene polymorphism with progression of Type 2 Diabetes Mellitus in Basrah Province-Iraq

Authors: Saja Majid Hamid --- Wafaa Sadoon Shani
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2018 Volume: 59 Issue: 2B Pages: 819-826
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

The study was aimed to investigate the association between IL-10 (-592A/C) gene polymorphism with the progression of type 2 diabeteis mellitus in Basrah Province. This study included (100) subjects (30) person as a control group and (70) patients with T2DM. The patients were distributed as two groups according to Their gender and duration of the disease: group for short duration (≤ 5 years) and group for long duration( >5 years). Lipid profile and glucose concentrations were measured by COBAS analyzer while IL-10 (-592A/C) gene polymorphism was genotyped by using (RFLP-PCR) technique. CC genotype frequency showed a significant decrease while CA genotype revealed significant increase ( p≤0.05) in T2DM compared with controls .No significant differences were observed in the allelic frequencies between both groups. Gender and duration of diabetes didn’t show any significant differences. There is significant association between CA genotype with the risk of T2DM (OR=1.50, 95% CI=1.035-2.173). We concluded that IL-10 (-592A/C) gene polymorphism contribute in development of T2DM.

Keywords


Article
Association of sFas and sFas Ligand with Progression of Type 2 Diabetes Mellitus in Basrah Province

Author: Saja Majid Hamid, Wafaa Sadoon Shani
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2018 Volume: 15 Issue: 2 Pages: 135-138
Publisher: Babylon University جامعة بابل

Loading...
Loading...
Abstract

Objective: This study aimed to assess the role of Fas/Fas ligand (FasL) system in the progression of type 2 diabetes mellitus (T2DM).Materials and Methods: This study included 100 participants – 30 persons as a control group and 70 patients with T2DM (35 males and 35 females)and their ages were ranged from 40 to 70 years. The patients were distributed into two groups according to gender and duration of the disease:newly diagnosed group for short duration ≤5 years and chronic diagnosed group for long duration >5 years. Serum sFas and sFasL levelswere measured by enzyme‑linked immunosorbent assay technique, and also, lipid and glucose profile were measured by COBAS analyzer.Results: The results revealed a significant (P ≤ 0.05) decrement in the levels of FasL in T2DM than controls while the levels of Fas wereincreasing significantly (P ≤ 0.05) in T2DM than controls. Hemoglobin A1c (HbA1c) and fasting blood glucose were negatively correlatedwith FasL, while high‑density lipoprotein was positively correlated with it, and whereas HbA1c positively correlated with Fas, the genderand duration of disease did not show any correlation with the disease. Conclusion: Our findings suggest that hyperglycemia causes increasein Fas levels which lead to dysfunction of pancreatic β‑cell in T2DM.


Article
Increased Transforming Growth Factor‑β and Interleukin‑17 Transcripts in Peripheral Blood of Breast Cancer Patients with Different Clinical Stages

Author: Hadeel Saleem Touma, Wafaa Sadoon Shani
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2018 Volume: 15 Issue: 2 Pages: 145-149
Publisher: Babylon University جامعة بابل

Loading...
Loading...
Abstract

Background: Currently, cancer as major problem around the world threatens human health and has a high incidence in developing countries.Many reports have showed that there is a dysfunction in the immune system of cancer patients. Interleukin‑17 (IL‑17) and transforming growthfactor‑β (TGF‑β) are increased in the generated through cancers, and IL‑17 is considered as a pro‑inflammatory cytokine; IL‑17 is an angiogenicfactor and promotes tumor growth. Materials and Methods: In this study TGF‑β and IL‑17, mRNA gene expression has been measuredin peripheral blood of 35 breast cancer patients and 35 normal age‑matched women using quantitative real‑time polymerase chain reactionmethod with master mix reaction containing SYBER Green. β‑actin gene was used as housekeeping gene. Results: Our data demonstrateda significant upregulation of TGF‑β and IL‑17 gene expression (P ˂ 0.05) in patient’s peripheral blood compared to normal healthy control.Levels of genes expression were higher in the breast cancer group compared to the control group. Conclusion: TGF‑β and IL‑17 expressioncould be used as a diagnostic marker for detecting breast cancer.


Article
Levels of Immunoglobulins and complements in sera of patients with toxoplasmosis

Author: Wafaa Sadoon Shani*, Bushra Hussein Shnawah*, Nabel Elia Wahedah
Journal: basrah journal of science البصرة للعلوم ISSN: 18140343 Year: 2012 Volume: 30 Issue: 1 B english Pages: 72-77
Publisher: Basrah University جامعة البصرة

Loading...
Loading...
Abstract

Eighty Seven sera of infected women with toxoplasmosis was tested to determined the levels of IgG, IgM, IgA, C3 and C4 by using single radial immunodiffusion technique and compared with the control sera of non infected subjects. The results showed that there was arising in the titer of IgG, IgM, C3 and C4 whereas IgA titer not recorded any arising when it was compared with the sera of control group

Keywords


Article
Serum levels of IL – 4 , Immunoglobulines and complements during human amoebiasis
تركيز 4- IL والكلوبيولينات المناعية والمتمم في مصل الانسان المصاب بداء الاميبات

Author: Aliaa Abdul Hussien and **Wafaa Sadoon Shani علياء عبد الحسين* و وفاء سعدون شاني
Journal: basrah journal of science البصرة للعلوم ISSN: 18140343 Year: 2006 Volume: 24 Issue: 1B english Pages: 112-119
Publisher: Basrah University جامعة البصرة

Loading...
Loading...
Abstract

In this work ( 60 ) serum samples of patients suffered from diarrhea and infected with Entamoeba histolytica were examined to evaluate the IL – 4 concentration by ELISA method and the concentration of IgG , IgA , IgM , C3 and C4 by single radial immunodiffusion . Recent study revealed that there was an increasing in concentration of the studied cytokine ( IL – 4 ) in patients serum ( 29.11 ) pg/ml in comparasion with control group with a highly significant differences ( p > 0.01 ) . Moreover , the present results showed that the mean concentration of IgG and IgA in patients with intestinal amoebiasis was more than those of control group with significant differences ( p > 0.05 ) , while there was no significant differences in concentration of IgM between two studied groups , ( although there is an increasing in it's concentration in some patients more than the normal values ) .The data revealed significant differences ( p > 0.05 ) in concentration of C3 between two analyzed group . whereas a highly significant differences ( p > 0.01 ) in concentration of C4 was established between patients and control groups

تم في هذا العمل فحص 60 عينة مصل لمرضى يعانون من الاسهال ومصابين بـ Entamoeba histolytica لغرض تحديد تركيز 4- IL باستخدام فحص الامتصاصية المناعية المرتبطة بالانزيم وكذلك IgG ، IgA ، IgM ،C3 ، C4 باستخدام طريقة الانتشار المناعي القطري البسيط .اوضحت الدراسة الحالية بان هناك زيادة في تركيز المدور الخلوي المدروس ) 4- IL ) في مصل المرضى (12- 29 ) بيكوغرام / مل بالمقارنة مع مجموعة السيطرة وبفروقات معنوية عالية( P < 0.01) اضافة لذلك اظهرت النتائج الحالية بان معدل تركيز IgG و IgA في المرضى المصابين بداء الاميبات المعوي اكبر من تركيزه في مجموعة السيطرة وبفارق (P< 0.05) بينما لم تظهر فروقات معنوية في بعض المرضى اكثر في تركيز IgM بين مجموعتي الدراسة بالرغم من ان هناك زيادة في تركيزه في بعض المرضى اكثر من القيم الطبيعية.اوضحت نتائجنا فروقات معنوية( P< 0.05) في تركيز C3 بين المجموعتين المدروستين بينما سجلت فروقات معنوية عالية في تركيز C4 بين المرضى ومجموعة السيطرة

Keywords

Listing 1 - 10 of 13 << page
of 2
>>
Sort by
Narrow your search

Resource type

article (13)


Language

English (10)


Year
From To Submit

2018 (3)

2014 (2)

2012 (4)

2011 (1)

2010 (1)

More...