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Evaluation of the efficacy of different storage media and different extra oral dry times on the viability of periodontal ligament cells of the avulsed teeth (In vitro study)

Authors: Yasameen A. A. Al- Bayati ياسمين البياتي --- Zainab A. Al- Dahan زينب الدهان
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2012 Volume: 24 Issue: 4 Pages: 139-144
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Tooth avulsion, one of the most severe forms of dental trauma, is characterized by completedisplacement of the tooth from its alveolar socket. It accounts for 0.5% to 16% of traumatic injuries in the permanentdentition. Avulsion of permanent teeth occurs most often in children 7 to 9 years old, an age when the relativelyresilient alveolar bone provides only minimal resistance to extrusive forces. The best way to preserve a tooth that hasbeen avulsed is to put it back into its socket as quickly as possible. Several storage media had been suggested topreserve the viability of the periodontal ligament cells. It was suggested to place the tooth under the patient'stongue in cases in which immediate replantation was not possible. Eagle's culture medium had also been used forstoring teeth. Also it was suggested to store the avulsed tooth in milk, Hank's balanced salt solution or saline. Chickenegg white may also prove to be a suitable storage medium for the avulsed teeth.Materials and methods: Eighty freshly extracted lower first premolars of special criteria were used in this study. Theteeth were divided into control groups in which the teeth were left to dry after extraction for 0 minute, 30 minutes, 4hours and 8 hours before being treated with the enzymes and experimental groups in which the teeth were left to dryafter extraction for 30 minutes, 4 hours and 8 hours before being immersed in a different experimental storage mediaand then treated with the enzymes to isolate the fibroblast cells of the periodontal ligament.Results: Results of this study showed that zero minute dry time was the best time for the control group in maintainingviable fibroblast cells followed by 30 minutes, 4 hours and 8 hours dry times. For the experimental groups, 30 minutesdry time was the best time followed by 4 hours and 8 hours dry times. Results of this study also showed that Hank'sbalanced salt solution was the best storage media that maintain viable fibroblast cells of the periodontal ligament ofthe extracted teeth with a significant difference compared to the control group and with no significant differencecompared to the other experimental storage media. Results also showed that the albumin of the chicken egg,honey and the glycerol were also good storage media with no significant difference than the control group.Conclusion: Hank's balanced salt solution was the best storage media in maintaining viable cells viability.Thirty minutes dry time was the best time in maintaining viable periodontal ligament cells followed by 4 hours and 8hours dry time after teeth avulsion


Article
Correlation between caries related bacteria in plaque and saliva in different age group children

Authors: Zainab J. Ja’far زينب جعفر --- Yasameen A.A. Al-Bayati ياسمين البياتي --- Ghada I. Taha غادة طه
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2012 Volume: 24 Issue: 3 Pages: 140-144
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Dental plaque contains bacteria that are both acidogenic and acidoduric. Different types ofstreptococci were identified in saliva. Although many bacterial subspecies have been shown to be associated withcaries, streptococcus mutans is still believed to be the most important bacterium in the initiation of this disease whilelactobacillus is correlated with the active caries episode. This study was conducted in order to estimate thecorrelation between caries related bacteria in plaque and saliva in different age group children.Materials and methods: Fifty three children aged 3-10 years old were chosen for this study. Recording of dental carieswas carried out by the dmfs index for primary teeth and DMFS index for the permanent teeth according to thecriteria suggested by the WHO. One ml of unstimulated (resting) whole saliva was collected from the children usingspitting method then diluted and applied on the surface of agar media specific for streptococcus mutans andlactobacilli growth. Dental plaque sample was taken from the buccal surface of the maxillary second primary molarby a clean toothpick and store in Epindorf tube which contain 1 ml. normal saline then inoculated in the samegrowth media that were used with the salivary samples. Colonies of the bacteria were counted with the aid ofdissection microscope (15 X) on the basis of their characteristic morphology.Results: Strong positive significant correlation was found between dmfs and ds components of the primary teeth,Positive results was found when correlating dmfs or ds with streptococcus mutans in dental plague while a negativecorrelation was found with lactobacilli. Negative correlation was found when correlating dmfs with streptococcusmutans in saliva while the relation is positive with lactobacilli. In dental plaque and in saliva there was a strongpositive highly significant correlation between the DMF and the DS. Correlation coefficient between DMFS with thebacterial counts of the caries related microorganisms (streptococci and lactobacilli) in the dental plaque and insaliva revealed weak, negative not significant correlations.Conclusion: The relation is not significant between the caries related microorganisms with each other in differentmedia; either the dental plaque or saliva

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