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HISTOLOGICAL AND MOLECULAR STUDY OF SPLEEN IN Japanese Quail UNDER THERMAL CONDITION

Author: Zainab A. Al-Ali Majdy Faisal Jalal yaseen
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: Print:18138497 E; 24108456 Year: 2017 Volume: 16 Issue: 1 Pages: 1-10
Publisher: Basrah University جامعة البصرة

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Abstract

The present study was conducted to determine the histological and Molecularchanges in the spleen of adult male Japanese quail after being exposed to effect ofthermal stress was recorded. This study included two groups, each group consistingtwelve male birds, the control group (A) was exposed to normal temperature for45days, while the group (B) exposed to temperature (42OC) for 45 days. Thehistological and molecular changes were studied during (15, 30 and 45) days of theexperiment, histological changes in spleen were represented by necrosis,degeneration hemorrhage and shrinkage fibrosis of lymphocytic nodules,cytoplasmic vaculation, infiltration of lymphocytes, metamorphosis of lymphocyte,nuclei pyknotic, dilation in the central vein of lymphatic nodules, edema, dilation ofsinusoids and congested blood vessels. Molecular examination showed that the heatshock protein (hsp70) gen is present in temperature group that is also found in thecontrol group. This confirms that the hsp70 gen is present in birds at normal andabnormal conditions.

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