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Effect of Gamma Radiation on Pathogenecity of Entamoeba Histolytica

Authors: Hayam K. Al-Masoudi --- Zainab H. Al-Saadi --- Saif A. Al-Shalaah
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2015 Volume: 12 Issue: 1 Pages: 301-309
Publisher: Babylon University جامعة بابل

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Abstract

The present study was conducted to test the effect of gamma radiation on the pathogenicity of E. histolytica in vivo by using radiation with doses (5, 10, 15, 20, and 25) rad to attenuation cyst of E. histolytica. The results revealed that mortality rate in laboratory animals (rabbits) decrease when increase the dose of attenuated E. histolytica cyst in comparative with positive control group (non – attenuated group) it reach (60% ,40%,40% and 20%) when give the rabbits attenuated E. histolytica cyst (5,10 ,15, and 20 ) rad. respectively . also we found no any mortality in rabbit which given attenuated E. histolytica cyst with dose (25) rad. also increasing doses of attenuating E. histolytica cyst (5,10,15,20 and 25) rad caused a some of histopathological changes in rabbits liver such as infiltration of inflammatory cell with degeneration of hepatic cells in addition to congested blood vessels and hydropic degeneration of hepatocyte in compare with liver of control group which gave the normal saline which appear normal shape and size. The results noted that this changes were reduce when increase the doses of radiation which decrease the pathogenecity of E. histolytica. We concluded that gamma radiation have good effects on reduce the pathogenicity of E. histolytica.

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