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Validation the Best, Most Accurate and Reliable Assay for Detection of Typhoid

Author: Zainab Khudhur Ahmed Al-Mahdi
Journal: Karbala Journal of Medicine مجلة كربلاء الطبية ISSN: 19905483 Year: 2013 Volume: 6 Issue: 1 Pages: 1561-1567
Publisher: Kerbala University جامعة كربلاء

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Abstract

yphoid fever is becoming an ever increasing threat in the developing countries.Cultivation of bacteria and serology (especially Widal test) give unacceptable levels offalse-negative and false-positive results, respectively.The aims of present work is development of a PCR assay that can target specific multiplegenes for rapid detection of Salmonella enterica serovar Typhi (S. typhi) .(which has 100%specificity for Salmonella typhi) was compared with Widal test and Salmonella typhiIgGIgM rapid test as well as Salmonella typhi IgG and IgM ELISA test during the first weekof illness of 50 suspected cases of typhoid.PCR primers for invasion, O, H and Vi antigen genes, invA, prt, and viaB were designed andused for the rapid detection of S. typhi by multiplex PCR.The respective figures of positivity for PCR, Blood culture, Widal test and S. typhi IgGIgMrapid test and Salmonella typhi IgG /IgM ELISA were 66%, 52%, 46%, 42% and 48%respectively. A control group of 20 healthy persons gave figures of 0%, 0%, 40%, 0% and0% respectively.The present study conclude that this PCR-based technique is not only absolutelyspecific, but also very sensitive and, therefore, much superior to Widal test, Blood culture S.typhi IgGIgM rapid test and Salmonella typhi IgG/IgM ELISA tests for the early diagnosisof typhoid.

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