research centers


Search results: Found 4

Listing 1 - 4 of 4
Sort by

Article
Immunological methods to develop camelid anti-cancer

Author: Zeyad A. Shabeeb
Journal: Mustansiriya Medical Journal مجلة المستنصرية الطبية ISSN: 20701128 22274081 Year: 2015 Volume: 14 Issue: 1 Pages: 46-50
Publisher: Al-Mustansyriah University الجامعة المستنصرية

Loading...
Loading...
Abstract

Background: The humoral immune response of the Camelidae is unique as these animals are the only known mammals that seem to possess functional homodimeric heavy-chain antibodies besides the classical heteromeric antibodies composed of heavy (H) and light (L) chains. Objective: to assess the ability of camel IgGs to react much better to the tumor associated antigen (TAA) than human IgGs and independently from the haplotype-restriction of the TAA complexes with MHC molecules. Materials and methods: The immunization of carcinoembryonic antigen (CEA) of two adult camels was carried out in the private farm at Sharjah – UAE for five weeks and then the specimens were collected and transferred to the central laboratory of the Sharjah University. The project was extended from April 2008 to April 2009. The specimens were fractionated by chromatography then were evaluated by using Elisa technique.Results: The results of CEA immunization of adult camel and its calf was determined by using indirect ELISA test. The ELISA readings showed positive response with camelid serum, urine, milk and calf serum. Negative results showed with pre-immunized serum from camel and its calf and from blocking buffer.Conclusions: This work succeeded in formulating TAA-specific highly reactive camelid IgG that are able to recognize only the cancerous cells and sparing the normal cells. These TAA-specific antibodies bound tightly to TAA antigens as well as to control carcino-embryonic antigen (CEA).

Keywords

CEA --- Camelid IgG --- TAA --- indirect ELISA --- VHH


Article
Detection of luxS Gene in Serratia marcescens and rpoS Gene in Enterobacter cloacae Isolates Using PCR Reaction

Authors: Zeyad A. Shabeeb --- Muthana B. Farhan --- Naba'a A. Muhammed
Journal: Al-Nahrain Journal of Science مجلة النهرين للعلوم ISSN: (print)26635453,(online)26635461 Year: 2018 Volume: 21 Issue: 1 Pages: 115-120
Publisher: Al-Nahrain University جامعة النهرين

Loading...
Loading...
Abstract

luxS and rpoS genes were involved in the regulation of some biological processes, which interfere with virulence of Serratia marcescens and Enterobacter cloacae bacterial species sequentially. luxS have an important activity in Quorum Sensing (QS) processes, but rpoS interfere with tolerance and resistance processes toward stress conditions correlating with its virulence. The study aimed to detect rpoS presence in E. cloacae and luxS gene presence in S. marcescens separately isolated from 16 leukaemia patients (4 of S. marcescens and 12 of E. cloacae), who characterised with bacteraemia by using primers designed for this purpose by using PCR technique. The results showed that all of the bacterial isolates of S. marcescens contain luxS gene, while no one of E. cloacae isolates show positive results for rpoS gene. The study concluded that S. marcescens has high virulence as a result of luxS presence, which controls Quorum sensing and prodigiosin formation and affecting host immunity. Moreover, the negative results of rpoS gene can be explained according to two possibilities: the gene can be found in the isolates with a copy differ from the registered sequences of the rpoS gene, or the gene did not present in those isolates with presence of alternatives offset its activities


Article
H.Pylori Associated with Immune Thrombocytopenic Purpura

Authors: Baan A. Mtashar --- Zeyad A. Shabeeb --- Zainab F.Ashoor
Journal: Iraq Joural of Hematology المجلة العراقية لامراض الدم ISSN: 20728069/25432702 Year: 2014 Volume: 3 Issue: 1 Pages: 42-46
Publisher: Al-Mustansyriah University الجامعة المستنصرية

Loading...
Loading...
Abstract

Background: Idiopathic thrombocytopenic purpura (ITP) is a hematological disorder characterized by sensitization ofplatelets by autoantibodies leading to platelet destruction.). Recently, H. pylori has been found to be associatedwith ITP and its eradication has shown improvement in platelet countObjective: To determine the association of Helicobacter pylori infection in patients presenting with idiopathicthrombocytopenic purpura (ITP).Materials and Methods: From October 2012 to March 2013, fifty adult patients with ITP and fifty age and sex matchedhealthy controls were investigated for the presence of H. pylori infection by Helicobacter pylori detection IgGand IgM antibody using ELISA method.Results: H. pylori IgM and H. pylori IgG: twelve patient (22%) and thirty nine patients (78%), of ITP patients had apositive expression H. pylori IgM and H. pylori IgG, respectively compared with three (6%) and thirty one (62%)patient in the control group. There was statistically significant difference between the mean of ITP patients andhealthy control groups.Conclusion: The study confirms the existence of an association between H. pylori infection and ITP.

Keywords

H.pylori --- ITP


Article
10.THE ROLE OF COLLAGEN BINDING ASSAY IN CLASSIFICATION OF VON WILLEBRAND DISEASE

Loading...
Loading...
Abstract

Background: von Willebrand disease (VWD) is the most common, genetically, hereditary and clinically heterogeneous bleeding disorder caused by qualitative or quantitative defects in von Willebrand factor (VWF) that leads to imperfect VWF interaction between platelets and injured blood vessel wall which deteriorate primary hemostasis.Objective: To assess the role of collagen binding assay in the classification of VMD.Methods: A cross sectional study was conducted on 52 suspected known patients with VWD with no consideration to their age or gender who attended the National Center of Hematology. They were submitted to von Willebrand factor antigen (VWF:Ag), factor VIII (FVIII), ristocetin co factor assay (VWF:RCo), ristocetin induced platelet aggregation (RIPA), and collagen binding assay (VWF:CB) at the same center.Results: The patients mean age was 14.42 ±1.64 years, median 10 years, and range of 1-40 years. More than half of cases below 10 years with M:F ratio 1:1.26. Two out of 9 cases of subtype I was diagnosed as subtype II, 4 out of 7 cases of subtype II sub classified as subtype2M and the rest 3 cases sub classified as 2A, 11 out of 36 of subtype III diagnosed as 8 cases for subtype I, and 3 cases for subtype II.Conclusion: VWF:CB assay is an important and effective supplementary test, in addition to three test panel FVIII, VWF:Ag, VWF:RCo, and multimere analysis, for sub classification of VWD. VWF:CB assay has a role in reclassified VWD patients with highly variable clinical presentation and laboratory values. Type III VWD is most frequent diagnosed type among symptomatic patients due to high consanguinity rate which detected earlier with severe bleeding tendency.Keywords: Von Willebrand disease, collagen binding assay, VWF:RCo. Citation: Suha K. Jabbar, Subh S. Al-Mudallal, Zeyad A. Shabeeb, Yusra G. Al-Obaidy, Hind S. Al-Mamoori. The role of collagen binding assay in classification of von Willebrand disease. Iraqi JMS. 2017; Vol. 15(2): 175-180. doi: 10.22578/IJMS.15.2.10

Listing 1 - 4 of 4
Sort by
Narrow your search

Resource type

article (4)


Language

English (3)


Year
From To Submit

2018 (1)

2017 (1)

2015 (1)

2014 (1)