research centers


Search results: Found 3

Listing 1 - 3 of 3
Sort by

Article
Effect of cryopreservation on some sperm parameters of infertile patients
تاثير الحفظ بالقري على بعض معايير النطف للمرضى العقيميين

Authors: محمد باقر محمد رشاد --- اسراء مجيد رشيد
Journal: Iraqi Journal of Embryos and Infertility Researches المجلة العراقية لبحوث الأجنة والعقم ISSN: eISSN: 26166984 / pISSN: 22180265 Year: 2013 Volume: 3 Issue: 5 Pages: 9-15
Publisher: Al-Nahrain University جامعة النهرين

Loading...
Loading...
Abstract

Background:Alt men have the natural desire to have their own biological children. Cryopreservation of male gametes is an important aspect of human fertility preservation. With the advancement in assisted reproductive technology, indication for sperm cryopreservation is expanding.Objectives:To evaluate the effect of cryopreservation by using Sperm Freeze TM kit on some sperm parameters for infertile patients.Materials and methods:Ninety five semen samples were taken from male patients. The seminal fluid analysis was performed according to the standard criteria of WHO (1999). The patients were divided into four groups depending on their sperm concentration and motility: Normozoospermic, oligozoospermic, asthenozoospermic and oligoasthenozoospermic patients. Cryopreservation in liquid nitrogen was done by using Sperm Freeze TM kit for the samples and thawing was performed after three months.Results:In general, the post-thawing study revealed a considerable highly significant (P <0.001) decrease in the sperm parameters which include the means of sperm concentration. sperm motility, progressive motility and normal sperm morphology as compared to that of pre-cryopreservation. Whereas the percentage of immotile sperm was highly significant (p<0.05) increase as compared to pre-cryopreservation in all groups except for oligozoospermic patients in which there was a significant (p<0.05) increase.Conclusions:After semen cryopreservation, all parameters were significantly decreased.


Article
Effect of cryopreservation on DNA normality of mice epididymal sperms following in vitro preparation with pentoxifylline and Glycyrrhiza glabra
تاثير الحفظ بالقري على طبيعة الحامض النووي للنطف البربخية للفئران بعد التحضير في الزجاج باستخدام البنتوكسيفلين وعرق السوس

Authors: saad s aldujaily --- مهد ساجت عويد --- عبد الحسين الفيصل
Journal: Iraqi Journal of Embryos and Infertility Researches المجلة العراقية لبحوث الأجنة والعقم ISSN: eISSN: 26166984 / pISSN: 22180265 Year: 2013 Volume: 3 Issue: 5 Pages: 24-30
Publisher: Al-Nahrain University جامعة النهرين

Loading...
Loading...
Abstract

Background:Many studies conducted on the pentoxifylline (PX) and Glycyrrhiza glabra (G.glabra) as motility I stimulants showed positive effect on sperm preparation in vitro improving the progressive forward movement. However, it was not known the impact of these stimulants on the nature of | genetic material, especially after cryopreservation.Objective:The experiment was designed to find out any harmful effect of a medium containing a mixture of PX and aqueous extract of G.glabra on the sperm DNA before and after cryopreservation.Materials and Methods:Fifty mature fertile male mice 812-weeks old were used. Epididymal sperms were obtained from caudal region and prepared in vitro by direct activation technique using four media namely;PX alone, G.glabra alone, a mixture of PX with G.glabra and Ham's F-12 (as control medium). Certain sperm function characteristics were examined as well as evaluation of DNA integrity | by acridine orange test and comet assay before and after cryopreservation.Results:The results showed a highly significant (P<0,001) improvement in the sperm forward movement, with a highly significant (P0.001) decrease in DNA abnormality using acridine orange test | and comet assay, following in vitro activation by the four media compared to before activation. Following in vitro activation and cryopreservation, the results of epididymal sperm showed a highly significant (P<0.001) decrease in DNA abnormality by using PX medium alone, G.glabra medium alone and the mixture of PX and G.glabra compared to control medium and the results before cryopreservation.Conclusion:According to the results of present study, it has been found no toxic effect of medium containing PX and/or G.glabra on the epididymal sperms DNA after activation in vitro and cryopreservation using acridine orange test and comet assay. The best result obtained when using a medium containing a mixture of PX+ G.glabra together. These data could be applied for preparation and activation of epididymal and testicular sperms obtained from obstructive azoospermic men.


Article
Evaluation of Different Cryopreservation Protocols of the Testis Using 8-Hydroxy 2-Deoxyguanosine as Marker of DNA Damage.
تقييم الانظمة المختلفة لحفظ الخصية بالقري باستخدام 8 هيدروكسي 2 دي اوكسي كوانوسين كمؤشر لضرر الدنا

Authors: استبرق عبد الرسول الكروي --- مصطفى جواد خليل --- انعم رشيد الصالحي
Journal: Iraqi Journal of Embryos and Infertility Researches المجلة العراقية لبحوث الأجنة والعقم ISSN: eISSN: 26166984 / pISSN: 22180265 Year: 2013 Volume: 3 Issue: 5 Pages: 16-23
Publisher: Al-Nahrain University جامعة النهرين

Loading...
Loading...
Abstract

Background:Chemotherapy and radiotherapy can destroy or severely reduce spermatogenesis and thereby jeopardize fertility in the long term. There is still no medical treatment that guarantees fertility preservation after chemo- and radiotherapy. Now with recent improvement of assisted reproductive technologies (ART) and possibility of using testicular spermatozoa or epididymal spermatozoa at in vitro fertilization (IVF) by intracytoplasmic sperm injection (ICSI), cryopreservation of testicular tissue is an option in fertility preservation for males who will lose spermatogenic cells as a result of chemo-and radiotherapy and for males with azoospermia.Objective:This study is an attempt to evaluate oxidative DNA damage in the mice testicular tissue after cryopreservation/thawing cycle when using different types of cryoprotectants as well as to investigate the changes that occur in mice testicular tissue after cryopreservation/thawing cycle as a model for human being.Methods:Fifty mature fertile male mice between 8- 12 weeks old were used in the current study. For each mouse, one testis was evaluated histologically and immunohistochemically (using 8-Hydroxy 2>-Deoxyguanosine as marker of oxidative DNA damage) without cryopreservation (control group). The other testis was evaluated histologically and immunohistochemically (using 8-Hydroxy 2>-Deoxyguanosine as marker of oxidative DNA damage) after six weeks of cryopreservation using different types of cryoprotectants (cryopreserved group).Results:The microscopically observation of slides obtained from cryopreserved testis differs according to the type of cryoprotectant (glycerol, 1,2 propanediol and dimethylsulfoxide), in the dimethylsulfoxide group, tissue sections showed no major differences versus control group, but in the histological sections obtained from tissue cryopreserved by glycerol as cryoprotectant showed moderate morphological and structural changes as compared with the control group. While, the tissue samples subjected to 1,2 propanediol as cryoprotectant displayed the severest morphological and structural changes as compared with the control group.Immunchistochemical results of the present study showed a highly significant (P<0.001) increase in oxidative DNA damage in the cryopreserved testis (46.78%, 63.45% and 32.59% represent cryoprotectant glycerol, 1, 2 propanediol and dimethylsulfoxide, respectively) compared with control group (19.27%) after six weeks of cryopreservation.Conclusion:From the results of the current study, it was concluded that there was alteration in testis histology after cryopreservation/thawing cycle and increase the level of oxidative DNA damage after cryopreservation/ thawing cycle. As well as dimethylsulfoxide cryoprotectant provide good protection for testis histology and DNA.

Listing 1 - 3 of 3
Sort by
Narrow your search

Resource type

article (3)


Language

English (3)


Year
From To Submit

2013 (3)