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Influence of Cold Atmospheric Plasma on Acinetobacter baumannii
دراسة تأثير البلازما الباردة على بكتريا الراكدة البومانية

Authors: Reyam Radi Att ريام راضي عطا --- Halah Al Haideri هاله الحيدري --- Hamid H Murbat حامد مربط
Journal: Baghdad Science Journal مجلة بغداد للعلوم ISSN: 20788665 24117986 Year: 2019 Volume: 16 Issue: 1 Supplement Pages: 151-161
Publisher: Baghdad University جامعة بغداد

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Abstract

A. baumannii is an aerobic gram negative coccobacilli, it is considered multidrug resistance pathogen (MDR) and causes several infections that are difficult to treat. This study is aims to employ physical methods in sterilization and inactivation of A. baumannii, as an alternative way to reduce the using of drugs and antibiotics. Cold Atmospheric Plasma was generated by one electrode at 20KV, 4 power supply and distance between electrode and sample was fixed on 1mm. A. baumannii (ATCC 19704 and HHR1) were exposed to Dielectric Barrier Discharge type of Cold Atmospheric Plasma (DBD-CAP) for several periods of time (15, 30, 45, and 60 sec.) . After sterilization test, several methods were done to analyze the effect of DBD-CAP on bacterial morphology, proteins and DNA. Change in morphology was assessed by cover slid method. Damaged DNA was investigated by PCR technique, and DNA sequencing. The impact of DBD-CAP on the entity of proteins was detected by SDS-PAGE. The observed inactivation of bacterial colony on agar plates has been quantified by measuring the inactivation diameter.The important conclusion that HHR1 more resistance to DBD-CAP than ATCC 17904 because it is more virulence than standard strain; thus, the growth of both strains is largely affected by plasma and this influence is increased by increasing the time of exposure, also the plasma affects the DNA especially on standard strain as it is explained in sequencing result, so it causes more deletion in DNA sequence. In addition, plasma also has been showed to damage proteins and morphology thus, the bacterial cells transform from cocco-bacillus to bacillus.

تهدف هذه الرسالة الى توظيف طرائق فيزيائية في معالجة الراكدة البومانية وذلك لغرض التقليل من استخدام الادوية والعقاقير. تضمنت التجربة تعريض سلالتين من الراكدة البومانية ( السلالة العالمية والسلالة المحلية) الى البلازما الباردة بقدرة 20 كيلو فولت وعلى الاوقات التالية (15 و30 و45 و60) ثانية, ثم بعد ذلك اجريت العديد من الاختبارات لغرض دراسة تأثير البلازما على البروتين والحامض الرايبوزي منقوص الاوكسجين وايضا على الشكل الخارجي, ان تأثير البلازما على نمو البكتريا تم حسابه عن طريق قياس قطر التثبيط. أثبتت النتائج ان قطر التثبيط يزداد بزيادة زمن التعرض الى البلازما ايضا اثبتت ان السلالة المحلية هي اكثر مقاومة الى البلازما من السلالة العالمية.

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