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The study was conducted to detect Infectious Bronchitis Virus (IBV) in commercial chicken farms of Middle Euphrates, the technique have been used throughout the study protocol, Enzyme Linked Immunosorbent Assay (ELISA), Real Time Reverse Transcription Polymerase Chain Reaction (RT-PCR) and histopathological study. (172) serum samples were collected from clinically infected vaccinated broiler farm and (29) serum samples were collected from clinically infected non-vaccinated broiler farm, and screened for the presence of IBV antibodies by ELISA.75serum samples from 172 samples were positive ,(43.6%) distributed as followed 43 (61.4%) from Hella, 23 (36.5%) from Najaf and 9 (23.07%) from Diwaneyah ,29 serum samples collected from clinically infected non-vaccinated broiler flocks, were 5 (17.2%) serum positive samples for IBV. (75) tissue samples from clinically infected vaccinated broilers distributed were submitted for rRT-PCR technique, the results were as followed42 (56%) have been detected IBV, while 30 (71.4%) was positive in ELISA, and (15) samples from clinically infected non-vaccinated broilers showed 8 (53.3%) IBV were detectable while 5 (62.5%) was positive in ELISA. Also the research include study the histopathological changes of trachea, lung and kidney of infected birds which were positive for rRT-PCR.

أجريت هذه الدراسة للكشف عن فايروس التهاب القصبات المعدي في مزارع الدجاج التجاري لمناطق الفرات الاوسط عن طريق اختباري الاليزا وتفاعل سلسله البلمرة المنعكس, حيث تم جمع 172 عينه مصل دم من مزارع الدجاج المصاب و الملقح أجرى عليها فحص الاليزا وكانت النتيجه172عينه موجبه وبنسبه (43.6%) وزعت كالتالي 70 (61.4%) من الحلة و63 (36.5%) من النجف و9 (23.7%) من الديوانية. كما تم اجراء اختبار تفاعل سلسله البلمرة المنعكس على 75 عينه من مزارع الدجاج الملقح لأجراء الاختبار وكانت 75عينه موجبه وبنسبه (56%) والتي فحصت مسبقا باختبار الاليزا ظهرت 42 (71.4%) عينه موجبه, وكذلك جمع 15 عينه من مزارع الدجاج الغير ملقح وكانت 8 (53.3%) عينه موجبه بفحص rRT-PCR والتي فحصت سابقا باختبار الاليزا وظهرت 5عينه موجبه (62.5%). كما شملت الدراسة ايضا دراسة التغيرات المرضية الي تطرا على عينات القصبة الهوائية, الكلية, والرئة للدجاج اللحم للعينات الموجبة عند تفاعل سلسله البلمرة المنعكس والاليزا.

chickens --- Elisa --- rRT-PCR --- Middle Euphrates --- Infectious Bronchitis --- IBV --- الدجاج ،اختبار الاليزا --- تفاعل سلسله البلمرة المنعكس --- الفرات الأوسط --- التهاب القصبات المعدي