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A Study of The Degradation of Atrazin and 2,4-D Herbicide by Mutagenic Bacterial Isolates
2,4- بعزلات بكتيرية مطفرة D و Atrazin دراسة تحلل المبيدين

Author: Kareem Ubed Hasan كریم عبید حسن
Journal: AL-Qadisiyah Journal For Agriculture Sciences مجلة القادسية للعلوم الزراعية ISSN: Online ISSN: 26181479 Print ISSN: 20775822 Year: 2012 Volume: 2 Issue: 2 Pages: 69-78
Publisher: Al-Qadisiyah University جامعة القادسية

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Abstract

Microbial isolates are isolated from 25 agricultural soil samples contaminated byherbicide. Their ability of degradation atrazin and 2,4-D herbicides areexamined . Two isolates are chosen according to ability for degradation , thenisolated and identified by using numbers of culture media and biochemical testsResults have indicated that the bacteria are Escherichia coli (B6) andPseudomonas fluorescens (B13) , which have been incubated with a number ofherbicides concentrations (5.0 -17.5) mg L-1 from atrazin and (5.0 -20.0) mg L-1from 2,4-D.Results have shown the degradation increases with increasing the concentrations(65 ، 60)% for isolate B6 , B13 respectively with 12.5 mg L-1 of atrazin and (73،61)% of 2,4-D with 15.0 mgL-1 of atrazin and (73،61) % of 2,4-D with 15.0mgL-1 after five days incubation.Chemical mutagen (Nitrosoguanidine) 500 μgmL-1 used to mutant bacteria cell with time (0.5 , 1.0 , 1.5 and 2.0) hr. one houris enough to do that , there fore rasio degradation increases (93 , 89) % Atrazinof (B13C6 , B6C3) respectively in 12.5 mgL-1 and (97 , 93) % , 2,4-D in 15.0mgL-1 . Achridin orange and sodium Dodecyl sulphate (SDS) are used to curinedbacteria plasmids by 2500 μg mL-1 from (SDS) and 200 μg mL-1 from (Achridinorange). The results show (B13C6 , B6C3) became curing cells.

252,4- . اختیرت عزلتین بكتیریتین قادرتین على تحلل المبیدین ، عزلت وشخصت D و Atrazin مبیديباستعمال الاوساط الزرعیة والاختبارات الكیموحیویة.B6 Esherichia coli :B13 Pseudomonas flurescance. 2,4-D 1- . (20.0- 17.5 ) ملغرام. لتر- 1 لمبید الاترازین و ( 5.0 - المبیدین ( 5.0و B13 %( 70 و 60 )ً1- . 12.5 B6. 73 و 61 )% عند التركیز 15.0 . ل - 1 ) 2,4-D500 ( )2.0 ) ساعة. ، 1.5 ، 1.0 ، مایكروغرام. مللتر- 1 بمدد زمنیة مختلفة ( 0.593)على التوالي عند التركیز 12.5 ملغرام. لتر- 1 (B6C و 3 B13C و 89 )% لمبید الاترازین للعزلات ( 6. 1- . 15.0 2,4-D %( و ( 97 و 93(B6C و 3 B13C البكتیریة ( 6(SDS) ( ) ( ) مادةو (SDS) 1- . 2500 (B6C و 3 B13C6)200 مایكروغرام مللتر- 1 من صبغة الاكردین البرتقالي

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