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The association of anticardiolipin antibody IgM with first trimester recurrent abortions
العلاقة بين الجسم المضاد للدهون من نوع آي جي آم والإسقاطات المتكررة خلال أول ثلاثة اشهر من الحمل

Author: Dr. Mohammad Shnain Ali
Journal: KUFA MEDICAL JOURNAL مجلة الكوفة الطبية ISSN: 1993517X Year: 2010 Volume: 13 Issue: 2 Pages: 157-161
Publisher: University of Kufa جامعة الكوفة

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Abstract

AbstractBackground: Anticardiolipin antibody has a clearly documented association with recurrent abortions in patients with autoimmune diseases; however, the association of Anticardiolipin antibody with first trimester abortions is not clearly documented in patients with no evident autoimmune diseases.Aim: To estimate the prevalence of Anticardiolipin antibody IgM in patients with first trimester recurrent abortions and to find if there is any association between them.Materials and methods: A total number of 90 women were included, 45 had history of recurrent abortions(more than 2 abortions) and 45 were healthy control women with one or more than one delivery and no history of abortions. Blood samples from the 90 women were taken for investigations including blood group, blood sugar, blood urea, complete blood picture, ESR, general urine examination, TORCH test, VDRL test and Anticardiolipin antibody IgM by ELISA technique in which levels higher than 15 MCL unit are considered to be positive, while levels below 15 MCL unit are considered to be negative.Results: Out of 45 patients, 24.4%(n=11) had history of explained recurrent abortions, 9 of them due to toxoplasmosis and 2 of them were due to cytomegaloviral infection. while 75.6%(n=34) had no explained causes. Significantly raised levels of anticardiolipin antibody IgM was present in 11 out of 45 patients i.e 24.4% of patients while IgM levels were normal in 34 out of 45 patients i.e 75.6%. The total number of explained recurrent abortions was 11 (8 out of 11 had significantly high IgM antibody i.e 72.7% while 3 out of 11 had normal IgM levels i.e 27.3%). The total number of unexplained recurrent abortions was 34 ( 3 out of 34 had significantly high anticardiolipin antibody IgM i.e 8.8% while 31 out of 34 patients had normal levels i.e 91.2%) while the antibody was negative (normal) in the control group. Patients with significantly high levels of anticardiolipin antibody who had history of explained recurrent abortions are eight. Seven of them i.e 87.5% had positive antitoxoplasma antibody IgM and one of them i.e 12.5% had positive anticytomegalovirus antibody IgM. Conclusion: Anticardiolipin antibody IgM is not associated with first trimester recurrent abortions.

الخلاصةالخلفية: للجسم المضاد للدهون علاقة مثبتة مع الاسقاطات المتكررة في حالة وجود الامراض المناعية المكتسبة مع ذلك فان العلاقة غير مثبتة في حالة عدم وجود الامراض المناعية المكتسبة. الغرض: معرفة وجود او عدم وجود علاقة بين الجسم المضاد للدهون من نوع آي جي ام والاسقاطات المتكررة خلال اول ثلاثة اشهر من الحمل.المواد والطرق: تمت دراسة 90 حالة(45 من النساء الحوامل ممن لهم اكثر من حالتين من الاسقاطات اول ثلاثة اشهر من الحمل و 45 من النساء ممن لهم اكثر من ولادة طبيعية وليس لديهم أي حالة اسقاط كمجموعة تتمتع بالصحة). تم جمع عينات الدم والادرار لاجراء التحليلات المرضية التالية: فصيلة الدم,نسبة السكر في الدم, يوريا الدم,صورة الدم الكاملة,نسبة ترسيب الكريات الحمراء,تحليل الادرار العام, اختبار تورج, اختبار السفلس, والجسم المضاد للدهون من نوع آي جي ام بطريقة اليزة وتم اعتبار المستوى الاعلى من 15 وحدة ايجابيا والمستوى الاقل او يساوي 15 سلبيا. النتائج: وجدت نسبة 24.4%(عدد 11) ممن لديهم اسقاطات متكررة مفسرة(9 منهم بسبب داء القطط و 2 منهم بسبب السايتوميجالوفايروس)بينما نسبة 75,6%(عدد 34) ممن ليس لديهم اسباب لتفسير الاسقاطات المتكررة. وجد ان مستويات الجسم المضاد للدهون من نوع آي جي ام بطريقة اليزة عالية احصائيا لدى 11 من مجموع 45 مريضة أي 24.4%(8 مرضى ممن لديهم اسقاطات متكررة أي 72.8% و 3 مرضى فقط ممن لديهم اسقاطات غير مفسرة الاسباب أي 8.8%)الاستنتاج: لا توجد علاقة بين الجسم المضاد للدهون من نوع آي جي ام والاسقاطات المتكررة خلال اول ثلاثة اشهر من الحمل في حالة عدم الاصابة بالامراض المناعية.


Article
DNA Fragmentation in Male Couples of Females with Recurrent Spontaneous Abortions (RSA)
تكسر الدنا في ذكور الزوجات اللاتي تعاني من الاجهاض التلقائي المتكرر الغير معروفة الاسباب

Author: Hazim Ismaeel Al-Ahmed حازم اسماعيل الاحمد
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2016 Volume: 57 Issue: 1B Pages: 391-396
Publisher: Baghdad University جامعة بغداد

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Abstract

Comet assay Single cell electrophoresis assay (SCGE) is a very sensitive method to determine DNA damage caused by exposure to mutagenic and carcinogenic and environmental agents that effect couples infertility. The aim of project was to evaluate DNA damage in males couples of females with recurrent spontaneous abortions. Forty males couples of females with unexplained recurrent spontaneous abortions and 25 healthy fertile males (control group) were evaluated for semen quality by conventional semen parameters, and alkaline comet assay was applied for the two groups. The results showed that patients male couples of females with RSA had a significant higher DNA damage than in the control group. It was concluded that patients male couples of females with RSA had high score of comet assay which suffers from RSA.

تعد تحليل المذنب (تحليل الترحيل الكهربائي لخلية منفردة) طريقة حساسة جدا لتحديد الدنا المتكسر بسبب التعرض للمواد المسرطنة والعوامل البيئية الاخرى والتي تؤثر على الخصوبة. كان الهدف من هذه الدراسة تقييم الدنا المتكسر في ذكور الزوجات اللاتي عانين من الاجهاض التلقائي المتكرر.شملت الدراسة 40 ذكرا لزوجات عانين من الاجهاض التلقائي المتكرر و 25 ذكرا اصحاء وخصبين كمجموعة سيطرة، وتم تقييم السائل المنوي لهم باستخدام الطريقة الاعتيادية لتقييم السائل المنوي. وتم استخدام طريقة تحليل المذنب القاعدية لجميع المجاميع، حيث اظهرت النتائج وجود ارتفاع معنوي في نسبة الدنا المتكسر في مجموعة الاجهاض التلقائي المتكرر مقارنة مع مجموعة السيطرة. واستنتج من نتائج هذه بان نسبة تحطم الدنا لدى ذكور الزوجات اللاتي عانين من الاجهاض التلقائي المتكرر اعلى من الطبيعيين.


Article
ELFA and IFAT Techniques to Detect Chlamydial Infections in Baghdad Women and Its Effect on the Immunoglobulins Level.

Authors: Khamael M. Kadaem* MSc --- Raad K. Al-Husseinei --- Lamya A. AL-Janabi --- Mun'im M. Fathei
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 / 24108057 Year: 2009 Volume: 51 Issue: 2 Pages: 192-197
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Chlamydia trachomatis is one of the most common human pathogens and considered asone of the causative agents of STDs. This organism cause acute and recurrent pelvic infections andinfertility.Patients and Methods: Two hundred and seventy three females were included in the present study,attending infertility department, AL-Elwiya hospital, AL-Jarah private hospital, central public healthlaboratory and STDs clinic to whom IFAT, ELAF and immunoglobulins concentration were done.Results: Females were divided into three age groups <20; 20-39 and ≥40 years. Single and repeatedabortions were 44.9%, 55.1% respectively. Primary and secondary infertility were 55.6% and 44.4%.Higher abortions rate were in the age group 20-39 years which represents 31.5% and 41.6% in singleand repeated abortions. Primary and secondary infertility were high in the same age group whichrepresents 42.6% and 33.3% of the total investigated females, also the multipartners within the sameage group constitutes 56.7%.Chlamydial infections detected by IFAT technique constitute 12.8% whichwere represented as 14.6%, 11.1%, 36.7% and 5% in abortions, infertility, multipartners and fertilitywith no abortion groups respectively. While by ELFA technique the percentages were 12.4%, 9.3%,30% and 4% in the same mentioned groups.Immunoglobulins mean value in females with chlamydialinfections wee as follows IgG = 2102.1 mg/dl; IgA = 317.9 mg/dl and IgM = 272.5 mg/dl. Which weremore than the normal values of the immunoglobulins.Conclusions: Chlamydial infections were distributed largely among multipartners than other abortedfemales. High abortions were noted in age group 20-39 years. IFAT technique was more reliable thanELFA technique to detect chlamydial infections.IgG and IgM concentrations were higher than normal concentrations, while IgA remains normal.

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