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Anti leishmanial Activity of Methanolic extract of Juniperus excelsa berries and Acacia nilotica
التأثير المضاد للشمانيا للمستخلصات الكحولية لنباتي العرعر juniperus excels berries والصمغ العربي Acacia nilotica

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Abstract

This study was conducted from December 2012 to May 2013. Samples were taken from the lesions of patients attending Tikrit Teaching Hospital and diagnosed clinically by professional doctors in dermatological department in the hospital clinically identified as Leishmania sp. according to lesion characteristics form. Parasites Isolates were harvested at stationary phase of growth injected intradermaly of 40 BALB/c mice for each plant. There were significant differences (P<0.001) in numbers of parasite cells in most concentrations used for methanolic extract of Juniperus excelsa berries compared with the control group without agent, and the statistically analysis showed that there were no significant differences (P>0.05) in the numbers of parasite cells in all concentrations used for methanolic extract of Acacia nilotica compared with the control group without agent. Also there were significant decrease (P<0.01) in number of parasite cells in control group treated with Pentostam compared with control group without agent

اجريت هذه الدراسة للفترة من كانون الاول 2012 – ايار 2013, وتم اخذ العينات من مناطق الافة للمرضى الوافدين لمستشفى تكريت التعليمي وشخصت الافة سريريا" بواسطة الطبيب المختص في قسم الجلدية على انها Leishmania sp حسب خصائص الافة او المنطقة المتضررة . تم عزل الطفيلي عند طور الثبات للنمو وحقن الطفيلي تحت الجلد في 40 فأرا" من نوع BALB السويسرية لكل نبات. لوحظ وجود فروقات معنوية (P<0.001) في اعداد الطفيلي في معظم التراكيز المستخدمة من المستخلص الكحولي لنبات Juniperus excels berries مقارنة مع مجموعة السيطرة المعاملة بالطفيلي فقط , كما اظهر التحليل الاحصائي عدم وجود فروقات معنوية (p> 0.05) في اعداد الطفيلي جميع التراكيز التي استخدمت للمستخلص الكحولي Acacia nilotica مقارنة مع مجموعة السيطرة المعاملة بالطفيلي فقط. اظهرت الدراسات ايضا" نقصان معنوي (p<0,01) في اعداد الطفيلي المعالج بالعقار بينتوسام Pentosatam مقارنة مع مجموعة السيطرة المعاملة بالطفيلي فقط.


Article
Hemolytic Activity of Acacia nilotica & Achillea millefolium on Human Red Blood Cells (in vitro Study)

Author: Faten Rashid Hameed
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2017 Volume: 14 Issue: 4 Pages: 738 -742
Publisher: Babylon University جامعة بابل

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Abstract

In this study, five concentrations of methanolic extraction of Acacia nilotica (10, 20, 50, 100, 250 mg) and six concentrations of Achillea millefolium (25, 50, 75, 100, 150, 200 mg) were used to study the extent of their effect on the damage of Red blood cells walls, the study were tested for replicates of blood samples for each concentration matched by controlled blood samples which were not used with the alcohol extract. The absorbance was obtained using a spectral device of a wavelength of 540 nm. The results indicated that there was a marked increase in red blood cell decomposition with the increasing of the concentration for both plants extractions.

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