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Green Synthesis of Silver Nanoparticles Using Leaf Extract of Al-Rawag tree (Moringa oleifera Lamarck) Cultivated in Iraq and Efficacy the Antimicrobial activity.

Author: Esam J. Al-Kalifawi
Journal: Mesopotamia Environmental Journal بيئة وادي الرافدين ISSN: 24102598 Year: 2016 Issue: Special Issue A Pages: 39-48
Publisher: Babylon University جامعة بابل

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Abstract

In the present study, environment friendly and cost effective silver nanoparticles were synthesized using the leaves extract of Al-Rawag tree cultivated in Iraq as the reducing and capping agent. The nanoparticless were characterized using UV-visble, FT-IR, XRD, and SEM methods. The surface plasmon resonance peaks in absorption spectra for silver colloidal solution showed an absorption peak at 430 nm in a UV-visible spectrum. The functional biomolecules such as carboxyl groups present in the seaweed responsible for the silver nanoparticles formation were characterized by FT-IR. The XRD results suggested that the crystallization of the bio-organic phase occurs on the surface of the silver nanoparticles or vice versa. The broadening of peaks in the XRD patterns was attributed to particle size effects and the average particles size about 30 nm which was calculated by using the Dubai-Scherrer equation. The silver nanoparticles synthesized by the help of Al-Rawag leaves extracts were scanned using SEM. It reveals that a silver nanoparticle seems to be spherical in morphology.The results shows that silver nanoparticles synthesized by leavesextract of Al-Rawag tree has effective antibacterial activities on the test isolates as indicated by the diameter of their zone of inhibition. The inhibition zone was 14 mm for Enterobactercloacae and Escherichia coli, 20 mm for Klebsiella pneumonia, 16 mm for Proteus mirabilis, Bacillus sp. and Streptococcus spp.12 mm for Pseudomonas aeruginosa, 18 mm for Staphylococcus aureus. The antimicrobial activity of leavesextract of Al-Rawag tree has effect against tested isolates less than Silver nanoparticles synthesis by it.The study revealed that the silver nanoparticles synthesis by using leaves extract of Al-Rawag tree could be as a therapeutic agent for human microbial infections.

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