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Article
HISTOCHEMICAL CHANGES ON SMALL INTESTINE MUCOSA INFECTED WITH GIARDIASIS IN WISTER ALBINO RATS
التغيرات الكيميائية النسيجية في الأمعاء الدقيقة المصابة بداء الجيارديات في الجرذان البيضاء

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Abstract

Brush border enzymes activity were decreased in group infected with giardiasis comparing with the control group, probably represent a direct effect of this parasite on the brush border of the enterocytes. The present study indicated that mucosal enzymes (alkaline phosphatase and lipase) levels were altered in infected rats after (1-2) weeks post infection compared to control group. concentration of both enzymes were increased during infection and this phenomenon was taken as mucosal marker for malabsorption.

سجلت النتائج حصول نقصان في الفعالية الانزيمية لانزيمات الحافة الحرة في الحيوانات المصابة بداء الجيارديات مقارنة مع مجموعة السيطرة.اشارت الدراسة الحالية الى ان مستويات انزيمات الطبقة المخاطية ( انزيم الفوسفاتيز القاعدي و اللايبيز ) تتغاير في الحيوانات المصابة خلال الاصابة تركيز كلا الانزيمين يزداد خلال (1-2) اسبوع بعد الاصابة وهذه الظاهرة اخذت كمؤشر على سوء الامتصاص .


Article
Separation with Estimation of High Molecular Weight Isoenzyme in Breast Cancer Patients
فصل وتقدير متناظر انزيم الفوسفاتيز القاعدي عالي الوزن الجزيئي في مرضى سرطان الثدي

Author: Dr. Sameeah Ibraheem Khamees
Journal: journal of the college of basic education مجلة كلية التربية الاساسية ISSN: 18157467(print) 27068536(online) Year: 2013 Volume: 19 Issue: 80 / علمي Pages: 837-842
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Total alkaline phosphatase (ALP) was measured in two groups of breast cancer patients, group 1 consisted of 27 patients with axillary metastases, group 2 consists of 4 patients with liver metastases .In addition , a group of control subjects was included . The results showed that total serum ALP was especially increased in group 2. ALP was purified from sera of patients with breast cancer by gel filtration on a sepharose CL-6B(106+30)cm and high molecular weight alkaline phosphatase isoenzyme (HALP) was isolated at pH 7.4.

تم قياس فعالية انزيم الفوسفاتيز القاعدي في مجموعتين من مرضى سرطان الثدي تتألف المجموعة الاولى من المرضى الذين تم انتشار المرض لديهم الى منطقة الابط والمجموعة الاخرى تتألف من مرضى سرطان الثدي الذين تم انتشار المرض لديهم الى منطقة الكبد ، بالاضافة الى ذالك تم قياس فعالية انزيم الفوسفاتيز القاعدي في الاشخاص الاصحاء . اضهرت النتائج ان فعالية انزيم الفوسفاتيز القاعدي قد زادت بصورة ملحوظة في المجموعة الثانية من مرضى سرطان الثدي . تم تنقية انزيم الفوسفاتيز القاعدي الموجود في مصل المرضى المصابين بسرطان الثدي بواسطة تقنية الترشيح الهلامي وتم عزل متناظر انزيم الفوسفاتيز القاعدي عالي الوزن الجزيئي وقياس فعاليته .


Article
The ability of Ca(OH)2 to facilitate bone formation by measuring the alkaline phosphatase level (Experimental study)

Author: Harith H Kaskos
Journal: Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان ISSN: 18121217 Year: 2006 Volume: 6 Issue: 2 Pages: 130-135
Publisher: Mosul University جامعة الموصل

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Abstract

Aim: To investigate the early events of bone regeneration of Ca(OH)2 by studying biochemistry levelof alkaline phosphatase (ALP) as guide for bone regeneration. Materials and Methods: The mandible of eight dogs was prepared by hole of 0.5×0.5 cm in depth and diameter in the apical to the molar teeth. This hole packed with Ca(OH)2 powder mixed with distilled water to become as a paste. Samples of blood were collected pre–operatively considered as control groups [measuring level of serum ALP in experimental animals before implanted Ca(OH)2], then at the 7, 10 and 15 day postoperatively considered as treated groups [measuring alteration of level of serum ALP in experimental animals after implanted Ca(OH)2] for biochemical analysis of the level of serum ALP. Results: There was a significant difference at the day 10 following the operation between the control groups and the treatedgroups, while there was no significant difference at the day 7,and day 15 postoperatively between the control and the treated groups. Also the results showed that there was a high significant difference between the different postoperative days. Conclusion: The (CaOH)2 powder mixed with distilled water have the ability to accelerate bone regeneration when it is placed in bone defect comparing to thatdefect filled with nothing.


Article
STUDYING THE EFFECTS OF SOME COMPOUNDS AND IONS ON Escherichia coli ALKALINE PHOSPHATASE ACTIVITY
دراسة تأثير بعض المركبات والأيونات على فعالية أنزيم الفوسفاتيز القاعديمن بكتيريا القولون

Author: KHALID .F.AL-RAWI خالد فاروق
Journal: Journal of university of Anbar for Pure science مجلة جامعة الانبار للعلوم الصرفة ISSN: ISSN: 19918941 Year: 2007 Volume: 1 Issue: 3 Pages: 58-69
Publisher: University of Anbar جامعة الانبار

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Abstract

The effect of some compounds and ions such as EDTA,Urea,KCN,2-Mercaptoethanol,BeCl2 and L-Phenyl alanine on the activity of Alkaline phosphatase extracted from Escherichia coli has been studied by using p-NitroPhenyl Phosphate as a substrate at a different concentrations (1.5„e10-1,3.0„e10-2,4.5„e10-3) M.,and the alkaline glycine regulating solution pH=10.5.Inhibition constant Ki and type of inhibition(competitive or noncompetitive)using Dixon plot for competitive & noncompetitive inhibition as well as the percentage of inhibition have all been calculated & I50 for different compounds.

KCN و Urea و EDTA تم دراسة تأثير بعض المركبات والأيونات مثلعلى فعالية أنزيم الفوسفاتيز القاعدي L-Phenyl alanine و BeCl 2- و 2 Mercaptoethanol وباستخدام Escherichia coli المستخلص من بكتريا الاشيريشيا القولون, 2-10 × 3 , 1-10 × كمادة أساس وبتراكيز مختلفة ( 1.5 p-NitroPhenylPhosphate(p-NPP)Ki وتم حساب ثابت التثبيط .pH = 3-10 ) مولاري والمحلول المنظم الكلايسين القاعدي 10.5 × 4.5ونوعية التثبيط (تنافسي أو غير تنافسي) باستخدام معادلة دكسون للتثبيط التنافسي والتثبيط الغير تنافسيI وكذلك تم حساب النسبة المئوية للتثبيط، كما تم حساب الثابت الذي يثبط 50 % من فعالية الأنزيم 502-10 ) مولاري . × للمركبات أعلاه بتراكيز مختلفة وباستخدام تركيز ثابت من المادة الأساس ( 3


Article
The effect of fluoride on osteoblast cell population after in vivo administration

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Abstract

Background: The osteoblast is characterized by its ability to synthesize a welldefined mineralized collagenous matrix, regulate the remodeling process bysynthesizing local hormone, specific molecules and alkaline phosphataseenzyme .The aim of this study was to evaluate the effect of Sodium Fluorideadministration in vivo on osteoblast cell.Materials and methods: Fourteen pregnant Sprague- Dawley rats were used, 8 ofthem received 100ppm Fluoride, others did not received and considered ascontrols. Bone biopsies from neonatal rat were studied histologically andhistochemically.Results: Active proliferation of osteoblast and their progenitor cells with activelocalization of alkaline phosphatase in neonatal jaw bone treated with SodiumFluoride.Conclusion: under the condition of the present study, the Sodium Fluoride can beused successfully to enhance bone formation.


Article
Inhibitory Effect Of Oxadiazooles And Thiadiazoles In Vitro On Serum Alkaline Phosphatase Enzyme Of Pregnant Woman

Authors: Tawfeeq F. R. AL-Auqbi توفيق العقابي --- Noor tha'ir Tahir Al-Khalidi نور ثائر الخالدي
Journal: Al-Mustansiriyah Journal for Pharmaceutical Sciences مجلة المستنصرية للعلوم الصيدلانية ISSN: 18150993 Year: 2012 Volume: 11 Issue: 1 Pages: 1-8
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Abstract:To assess the inhibitory effect of oxadiazole and thiadiazole derivativeson serum alkaline phosphatase level of pregnant in vitro. A cross-sectional studyon 34 pregnant women was carried out at Al-Kadhumia Teaching Hospital. The mechanism of Inhibitory effect of Oxadiazoles and Thiadiazoles derivatives in vitro on alkaline phosphatase enzyme of pregnant women due to thedephosphorylation reaction which block the ALP activity.Key Words: Oxadiazoles, Thiadiazoles, serum alkaline phosphatase

الخلاصة:لغرض تقییم التأثیر المثبط لمركبات الأوكسیدیازول والثیادیازول على مستویات أنزیم الفوسفیتیزالقاعدي في مصل الدم للنساء الحوامل مختبریا. صممت الدراسه بشكل دراسه مقطعیه على 34 امرأهحامل في مستشفى ألكاظمیه التعلیمي. تبین من الدراسه ان آلیه التثبیط لمركبات الاوكسادیازولوالثیادیازول لأنزیم الفوسفتیز القاعدي مختبریا تحدث نتیجة لتفاعل أزاله الفسفره والتي تعیق فعالیه أنزیمالفوسفتیز القاعدي.


Article
morphometry of the postnatal development of rat hippocampal capillaries

Authors: Anam rasheed al-salihi --- Akram abood Jaffer --- nahidh Abdul-shaheed Al- Jaberey
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2006 Volume: 48 Issue: 4 Pages: 445-453
Publisher: Baghdad University جامعة بغداد

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Article
Assessment of Alkaline Phosphatase, Salivary Flow Rate and Salivary Potential of Hydrogen in Relation to Severity of Chronic Periodontitis

Authors: Saif S. Saliem سيف سهام سليم --- Hussein A. Mousa حسين موسى
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2016 Volume: 28 Issue: 3 Pages: 126-131
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: The cells of periodontium contain many intracellular enzymes like (alkaline phosphatase ALP) that arereleased outside into the saliva and gingival crevicular fluid (GCF) after destruction of periodontal tissue. The aim ofstudy was to determine the activity of this enzyme in saliva and its relation to the salivary flow rate, PH and clinicalperiodontal parameters in patients with chronic periodontitis.Subject, Materials and methods: Sample population consist of 75 individuals ;divided into four groups , the first group(15):control subject, the second group (20):mild chronic periodontitis, the third group(20) moderate chronicperiodontitis and the fourth group (20) sever chronic periodontitis, Measurements of plaque index (PLI), gingival index(GI), bleeding on probing (BOP), probing pocket depth (PPD) and clinical attachment level (CAL), only male wereincluded and saliva was collected from them and subjected to biochemical analysis of the alkaline phosphataseenzyme (ALP), and also measurement of salivary flow rate(FR) and PH.Results: Statistical analysis of the results revealed the presence of a highly significant difference in the enzymaticactivity between healthy and chronic periodontitis subjects (mild, moderate, severe) with positive correlationbetween the activity of this enzyme and the clinical periodontal parameters, and negative correlation between thisenzyme and Salivary flow rate and pH.Conclusion: From this study it can be concluded that a number of markers show promise as sensitive measures ofdisease and the effectiveness of therapy. At this time enzymes such as alkaline phosphatase ALP, is goodbiochemical markers of screening chronic periodontitis. Also ALP can be used as a monitor for healthy individualsand patients with different periodontal diseases. Furthermore, analysis of saliva may offer a cost effective approachto assessment in controlling progression of chronic periodontitis in large populations


Article
Effects of light smoking on salivary levels of alkaline phosphatase and osteocalcin in chronic periodontitis patients

Authors: Lubaba A. Abdul Ameer لبابة عبد الامير --- Basima Gh. Ali باسمة غفوري علي
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2015 Volume: 27 Issue: 2 Pages: 110-114
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Chronic periodontitis is an inflammatory disease that affects the supporting tissues of the teeth and it’scommon among adults. Smoking is an important risk factor for periodontitis induces alveolar bone loss. Alkalinephosphatase enzyme is involved in the destruction of the human periodontium. It is produced by many cells such aspolymorphonuclear leukocytes, osteoblasts, macrophages and fibroblasts within the area of the periodontium andgingival crevice. Osteocalcin is one of the most abundant matrix proteins found in bones and the only matrix proteinsynthesized exclusively there. Smaller Osteocalcin fragments are found in areas of bone remodeling and are actuallydegradation products of the bone matrix.The purpose of this study was to evaluatethe effect of smoking on thesalivary alkaline phosphatase and Osteocalcin in subjects with chronic periodontitis compared to control subjects.Materials and Methods: Five ml of unstimulated whole saliva samples and full-mouth clinical periodontal recordings(plaque index, gingival index, bleeding on probing, probing pocket depth and clinical attachment level) wereobtained from study groups (25 light smokers and 33 non-smokerssubjects, both with chronic periodontitis) andcontrol groups (8 light smokers and 13 non-smokers subjects, both with healthy periodontium). All subjects weresystemically healthy males, with age range (30-50) years. Salivary Alkaline phosphatase and Osteocalcin levels weredetermined by Colorimetric and Enzyme-linked Immunosorbent Assays, respectively.Results: Smoker chronic periodontitis patients revealed non-significant differences in clinical periodontal parameterswith non-smoker counterparts (P o.o5) in terms of Plaque index, Probing pocket depth and Clinical attachment loss,with slight increase in plaque index value in smoker chronic periodontitis group(1.42±0.46) than non-smoker chronicperiodontitis group, while there were highly significant differences in terms of Gingival index and Bleeding onprobing(P ≤ 0.01).Osteocalcin levels were lower in smoker chronic periodontitis group (0.13±0.20) than non-smokerchronic periodontitis group (1.09±2.26) with significant difference (0.05 ≥ P > 0.01). Mean of Alkaline phosphataselevel was lower in smoker chronic periodontitis (11.14±4.53) than non-smoker chronic periodontitis (11.45±4.17) with anon-significant difference, while there was a significant difference inAlkaline phosphatase concentrations betweensmoker and non-smoker control groups.There were non-significant differences between smoker chronic periodontitisand smoker control groups in terms of Osteocalcin and Alkaline phosphatase concentrations. There were nonsignificantdifferences between non-smoker chronic periodontitis and non-smoker control groups in terms ofOsteocalcin and Alkaline phosphatase concentrations.Conclusion: Within the limits of this study, it may be suggested that suppression of salivary Osteocalcin levels bysmoking and slight increase in alkaline phosphatase in smokers groups, may explain the deleterious effects ofsmoking on periodontal health status.


Article
The Effect of Orthodontic Force on Salivary Levels of Alkaline Phosphatase Enzyme

Authors: Suha Ali Abdul Ameer سهى علي عبد الامير --- Akram Faisal Alhuwaizi اكرم فيصل الحويزي
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2015 Volume: 27 Issue: 4 Pages: 175-179
Publisher: Baghdad University جامعة بغداد

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Background: Orthodontic tooth movement is characterized by tissue reactions, which consist of an inflammatoryresponse in periodontal ligament and followed by bone remodeling in the periodontium depending on the forcesapplied. These processes trigger the secretion of various proteins and enzymes into the saliva.The purpose of thisstudy was to evaluate the activity of alkaline phosphatase (ALP) in saliva during orthodontic tooth movement usingdifferent magnitude of continuous orthodontic forces.Materials and Methods: Thirty orthodontic patients (12 males and 18 females) aged 17-23 years with class II division Imalocclusion all requiring bilateral maxillary first premolar extractions were randomly divided into three groupsaccording to the magnitude of the force application (40, 60 and 80gm). A sectional fixed appliance was bondedand designed to give labial force to the maxillary first premolar for three weeks. Unstimulated saliva was collectedfrom the patients before force application, then 1 hour after force application, followed by 1 day, 7 days, 14 daysand 21 days.Salivary levels of ALP were measured using spectrophotometer and compared with the baseline level.Results: The results revealed that ALP enzymelevel increased with increasing magnitude of orthodontic force (from 40to 80gm). This was statistically insignificant after 1 hour and 1 day of force application, but significant after 7, 14 and21 days.The ALP level significantly increased from baseline after 7 days of force application and peaked at 21 daysfor all the three force levels.Conclusions: The ALP level reflect the biological activity that takes place in the periodontium during orthodontictooth movement, and therefore they can be used as a diagnostic tool for monitoring of correct orthodontic toothmovement in clinical practice

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