research centers


Search results: Found 5

Listing 1 - 5 of 5
Sort by

Article
Association of Androgen Receptor Gene Polymorphisms at three SNPs and their Haplotypes with Severe Oligozoospermia Risk in Iraqi Patients

Author: Mustafa F. Khadhim, Ismail A. Abdul-Hassan
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2017 Volume: 16 Issue: 1 Pages: 16-28
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

Infertility in otherwise healthy males be caused by a variety of disorders. These include acquired, as well as heritable conditions. Genetic causes of male infertility are of special interest to reproductive biology, because these disorders can be passed on to the offspring, single nucleotide polymorphisms are considered as one of reason of male infertility Androgens are critical steroid hormones that determine the expression of the male phenotype. Their actions are mediated by a single androgen receptor (AR) which, upon ligand binding, translocate to the nucleus to regulate the expression of androgen-responsive genes. Mutations in androgen receptor gene may lead to a disturbance in the function of the androgen receptor which, in turn, can lead to several forms of infertility. The aim of this study was to determine the frequency of rs962458(SNP1), rs6152(SNP2) and rs2361634(SNP3)and test their associations with severe oligozoospermia risk .This study was conducted using 50 severe oligozoospermia patients and 50 apparently healthy subjects (Control). The frequency of GA genotype of SNP2 was significantly (p≤ 0.05) increased in patients versus control (10 and 0%, respectively, OR=0.561, X2=4.329),while the frequency of GG genotype of SNP2 was significantly (p≤ 0.01) decreased in patients versus control(84 and 100%, respectively, OR=0.871, X2=6.524). The results of TAA and TAG haplotypes were not significant. The frequency of TAG/TAG haplotype combination was significantly (p≤ 0.01) decreased in patients versus control(78 and 100%, respectively, OR=1.072, X2=8.35),while TAG/TAA haplotype combination frequency was significantly (p≤ 0.05)increased in patients versus control(14 and 0%,respectively, OR=0.664,X2=4.69). In conclusion, both GA genotype of rs6152A>G and TAG/TAA haplotype combination may be correlated with severe oligozoospermia risk in Iraqi patients.

Keywords

SNP --- Androgen receptor --- PCR --- Infertility.


Article
Effect Of Castration Methods On Gene Expression Of Androgen Receptor Gene In Skeletal Muscles Of Awassi Sheep
تأثير طريقة الاخصاء على التعبير الجيني لجين مستقبلات الاندروجين في العضلات الهيكلية لأغنام العواسي

Loading...
Loading...
Abstract

The effect of castration on skeletal muscle development in awassi sheep were studied at the gene expressed for androgen receptor. The result showed that live weigh for epididymectomy rams was heavier than control and orchidectomy rams, respectively. There was significant difference (p < .05). By using the (2^ΔCT) with reference gene method, the findings documented that there was higher up-regulated in mRNA expression for androgen receptor in splenius muscles cells for epididymectomy rams compare with interact rams. In contrast, there was down-regulated for mRNA expression for androgen receptor cells for orchidectomy rams. In conclusion the continuous producing of testes hormones lead to growth of skeletal muscles of wether's significant increase the growth of skeletal muscles of wethers by epididymectomy companied with other rams.

تم دراسة تأثير نوع الأخصاء على تطور العضلات الهيكلية في اغنام العواسي من خلال تعيين التعبير الجيني لجين مستقبلات الاندروجين, حيث أظهرت النتائج بان الوزن الحي للاكباش المخصية بطريقة ازالة جزء من البربخ كان وزنها اعلى من الاكباش في مجموعة السيطرة والاكباش المخصية بطريقة ازالة الخصية حيث انه كات هناك فرق معنوي (p < .05)كما واثبتت الدراسة بان هناك مستوى عالي للتعبير الجيني للحامض النووي الرايبوزي الناقل لمستقبلات الاندروجين في خلايا العضلة العنقية للاكباش المخصية بطريقة ازالة جزء من البربخ مقارنة مع الاكباش السليمة على العكس من ذلك فأن النتائج اظهرت أن التعبير الجيني لجين مستقبلات الاندروجين كان واطئ لنفس الخلايا في الاكباش المخصية بطريقة ازالة الخصية, من هنا نستنتج بأن استمرارية الخصية بانتاج الهرمونات يؤدي الى زيادة معنوية في نمو العضلات الهيكلية للاكباش المخصية بطريقة ازالة جزء من البربخ مقارنة مع بقية الاكباش الاخرى.


Article
Relation of elevated androgen to lipid profile in patients with polycystic ovary syndrome
العلاقة بين زيادة الهرمون الذكري واختلال مظهر الدهون في المريضات المصابات بمتلازمة تكيس المبايض المتعدد

Author: Sawsan Mohammad د. سوسن محمد
Journal: IRAQI JOURNALOF COMMUNITY MEDICINE المجلة العراقية لطب المجتمع ISSN: 16845382 Year: 2015 Volume: 28 Issue: 4 Pages: 207-211
Publisher: Al-Mustansyriah University الجامعة المستنصرية

Loading...
Loading...
Abstract

Background: Polycystic ovary syndrome is considered to be the most prevalent endocrinopathy resulting from anovulation and affects 5-10% of women. Both insulin resistance and hyperandrogenemia predispose polycystic ovary syndrome patients to atherogenic lipid profile. It is not clear however what role hyperandrogenism has on the development of the metabolic syndrome.Objective: To investigate the effect of elevated androgen level in patients with polycystic ovary syndrome on lipid profile.Methods: Between October 2012 and October 2013, forty polycystic ovary syndrome patients and forty healthy controls were recruited into this cross sectional study where the following parameters were determined for PCOS patients and compared to healthy control: Serum Follicle stimulating hormone , Luteinizing hormone level and testosterone levels. Serum lipid profile (total cholesterol, triglyceride, high density lipoprotein and low density lipoprotein) were measured by spectrophotometry by enzymatic colorimetric method. Linear regression analysis was used to evaluate the association between the lipid parameters and the testosterone level.Results: This study has showed that the luteinizing hormone (LH), Follicle stimulating hormone (FSH), and testosterone are significantly different between the two groups(8.4±1.16 vs. 7.02±1.8; p=0.002, 4.43±1.3 vs. 5.4±1.24; p=0.04, 2.104±1.161 vs. 0.82±0.32; p=0.001 respectively). Regarding lipid profile, Polycystic ovary syndrome patient display lower high density lipoproteins level (40.3143±7.5994 vs. 45.7272±11.348; p<0.001) and higher triglyceride level (168.6±34.6401 vs. 129.625±45.4125; p<0.001). In linear regression analysis total cholesterol was positively associated with testosterone level and High density lipoprotein cholesterol was negatively associated with testosterone level(regression coefficient = 0.313 for total cholesterol, r=0.409 for High density lipoprotein-cholesterol). No association was observed between Low density lipoprotein-cholesterol or triglyceride levels with testosterone level.Conclusion: Our results suggest that the characteristic hyperandrogenemia in women with PCOS were associated with dyslipidemia.

متلازمة تكيس المبايض المتعدد من اكثر الامراض شيوعا,وينتج من انعدام التبييض ويصيب 5-10 بالمئةمن النساء.مقاومة الانسولين وارتفاع الهرمون الذكري يعرض النساء المصابات بمرض تكيس المبايض المتعدد الى اختلال في مظهر الدهون,لكن تأثير ارتفاع الهرمون الذكري على مظهر الدهون ما زال غير واضح تماماغرض البحث: هو دراسة تاثير ارتفاع الهرمون الذكري على مظهر الدهون في المريضات المصابات بمرض تكيس المبايض المتعدد طريقة العمل : تم اختيار اربعين مريضة مصابة بمتلازمة تكيس المبايض المتعدد وتم مقارنتهن مع اربعين امراة غير مصابة. وتم قياس هرمون اللوتيني وهرمون المحفز للحويصلات والهرمون الذكري اضافة الى قياس مستوى الدهون بطريقة مقارنة الاطياف الضوئية.وتم مقارنتهن بنساء غير مصابات بمتلازمة تكيس المبايض المتعدد. النتائج: بينت هذه الدراسة انه يوجد فرق معنوي بين الهرمون اللوتيني وهرمون المحفز للحويصلات والهرمون الذكري بين المجموعتين(8.4±1.16 vs. 7.02±1.8; p=0.002, 4.43±1.3 vs. 5.4±1.24; p=0.04, 2.104±1.161 vs. 0.82±0.32; p=0.001 ( كذلك بينت هذه الدراسة عدم وجود فرق معنوي بين معامل الكتلة الجسدية والكوليسترول والدهون واطئة الكثافة وبينت وجود فرق معنوي (زيادة) في الدهون الثلاثية(168.6±34.6401 vs. 129.625±45.4125; p<0.001). ونقصان في الدهون عالية الكثافة(40.3143±7.5994 vs. 45.7272±11.348; p<0.001). وعند استخدام تحليل الارتداد الخطي وجدنا علاقة بين الهرمون الذكري و الدهون عالية الكثافة وعلاقة بين الهرمون الذكري والكوليسترول. الاستنتاج: وجد أن زيادة الهرمون الذكري يرتبط مع اختلال مظهر الدهون في المريضات المصابات بمرض تكيس المبايض المتعدد.


Article
Effect of Body Mass Index on Abnormal Ovarian Secretion Hormones among Iraqi Women with Polycystic Ovarian Syndrome (PCOS)

Authors: Alaa H. Badr --- Alaa H. Jawad --- Rania M. L. Faraj
Journal: Al-Nahrain Journal of Science مجلة النهرين للعلوم ISSN: (print)26635453,(online)26635461 Year: 2019 Volume: 22 Issue: 1 Pages: 40-45
Publisher: Al-Nahrain University جامعة النهرين

Loading...
Loading...
Abstract

Polycystic ovarian syndrome (PCOS) is the most common and complex endocrine disorder affecting women in the reproductive age. The etiology of this syndrome is not completely known, so there is a developing proof that supports genetic basis, PCOS has a strong familial preference. This study was carried out to investigate the effect of body mass index (BMI) on ovarian hormones in women with PCOS and healthy women. Eighty-three women aged (17-40) years were divided into two groups for testing (BMI less than 25 and more than 25). Hormonal study of Luteinizing hormone (LH), Androgen, Estradiol (E2) was done for each patient, the results of these hormones in patients compared with controls in both BMI groups with E2/T ratio showed a significant increase in patients women compared to controls in (p<0.05), While the results of Follicle stimulating hormone (FSH) and Total Testosterone (TT) showed an increase with not statistically significant. it has been concluded that the increase in BMI doesn’t show to have an opposite effect on FSH, Androgen, E2, and TT levels but the result of LH shows a highly significant increase in PCOS groups compared to controls when BMI˃25.

Keywords

Polycystic ovarian syndrome --- LH --- E2 --- FSH --- TT --- BMI --- ovarian hormones --- Androgen.


Article
Immunohistochemical Expression of Androgen Receptor (AR) and its Relationship with Clinicpathological Parameters in Breast Cancer
تعبير المناعة النسيجية الكيميائية لمستقبلات الاندروجين وعلاقته بالمعلمات الاكلينيكية في سرطان الثدي

Author: Wafaa Fadhil Hamad وفاء فاضل حامد
Journal: Al-Nisour Journal for Medical Sciences مجلة النسور للعلوم الطبية ISSN: 26640562 26640554 Year: 2019 Volume: 1 Issue: 2 Pages: 250-262
Publisher: Al-Nisour University College كلية النسور الجامعة

Loading...
Loading...
Abstract

Breast carcinoma is one of the most common malignant and the leading cause of cancer related death among women all around the world. Gene expression profiling characterized 4 major groups of BC, which classified patients into Luminal A, Luminal B, HER-2/neu enriched, and Triple Negative BC (TNBC). Among the members of the steroid receptor superfamily the role of estrogen and progesterone receptors (ER and PR) and HER2 are play a role in breast cancer as both predictive and prognostic markers and management of therapy. Androgen Receptor (AR) is a steroid nuclear receptor involved in complex signaling pathways that are thought to play a role in cell proliferation. Greater than70% of human breast cancers expresses the androgen receptor (AR) and varies significantly among molecular subtypes of breast cancer and its contribution to the progression of disease may differ depending on the stage. In this study, we aimed to examine the expression pattern of AR and its association with clinicopathological parameters and IHC markers.Methods: Immunohistochemistry (IHC) was performed on breast cancers using antibodies against androgen receptor (AR), esterogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor type-2 (HER2). The levels of expression were scored semiquantitatively.Results: AR positivity was noted in Thirty-four of the cases, whereas the other thirteen cases were negative. AR was significantly related to tumors histological type (p= 0.002), tumor grade (p= 0.002) and positive PR/HER2 status (p= 0.04). No statistical difference was demonstrated in AR expression in relation to tumor size (p= 0.12), lymph node status (p= 0.68) and expression of ER (p= 0.23). Conclusion: breast carcinomas express AR positivity more than ER, PR and HER2 status. In addition, the expression of AR correlated to lower tumor grade which could serve as a good prognostic factor and potential therapeutic target. However, this finding will need to be confirmed by large cohort studies.

يعد سرطان الثدي السرطان الاكثر شيوعا الذي يصيب النساء في مختلف انحاء العالم .وقد أجريت الدراسة على47 عينة من نساء مصابات بسرطان الثدي وكانت عبارة عن أنسجة مثبتة بالفورمالين ومطمورة بالبرافين على هيئة قوالب . استخدم طريقة كيمياء النسيج المناعية لتقييم تعبير المستقبلات لكل من HER-2 و الاستروجين و البروجيستيرون وكذلك Androgen لإيجاد العلاقة بين نتائج كيمياء المناعة النسيجية ومؤشرات التقدم لسرطان الثدي المتمثلة بعمر المريض ، حجم الورم ،النوع النسيجي وكذلك درجتة. حيث اظهرت النتائج بان 43 حالة من 47 اعطت التعبير الهرموني الموجب للمستقبل Androgen . كم أظهرت النتائج وجود فروقات معنوية مرتبطة بين مستوى التعبير للمستقبل Androgen مع نوع ودرجة الورم وكذلك كلا من البروجستيرون و HER-2 وبالمقابل فان تعبير Androgen الموجب يعد بمثابة نذير جيد وكهدف علاجي محتمل.

Listing 1 - 5 of 5
Sort by
Narrow your search

Resource type

article (5)


Language

English (3)

Arabic and English (2)


Year
From To Submit

2019 (2)

2017 (1)

2015 (2)