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Article
Molecular Detection of Some A.hydrophila Toxins and its Antibiotics Resistance Pattern Isolated From Chicken Feces in Thi-Qar Province (Iraq)

Authors: Muslim Dhaher Musa --- Waffa Abdulelah Ahmed2
Journal: Kufa Journal For Veterinary Medical Sciences مجلة الكوفة للعلوم الطبية البيطرية ISSN: 20779798 Year: 2017 Volume: 8 Issue: 1 Pages: 167-180
Publisher: University of Kufa جامعة الكوفة

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Abstract

The high isolation rate of virulent A.hydrophila from chicken represent an important public health concern especially when these bacteria exhibit increased antimicrobial resistance to medically important antibiotics, so this study was conducted to isolate A.hydrophila from chicken feces and investigate the presence of some toxins genes and determine their antibiotics resistance profile. Chicken feces were collected from different regions, throughout Thi-Qar province south of Iraq, in period extended from July 2015 to January 2016. A.hydrophila isolates were identified by morphological, biochemical and API20E. These isolated were subjected to PCR assay for confirmation, targeting 16S RNA-23SRNA Intergenic Spacers Region and for detection of important virulence genes including hemolytic toxin Aerolysin (Aero), Heat labile enterotoxin (Alt) and Heat stable enterotoxin (Ast). Twelve A.hydrophial were isolated and identified to species level among 23 Aeromonas spp isolated from chicken fecal samples with overall incidence rate (52.6%). Screening for virulence genes revealed that 10/12 (83.4%) were positive for Aerolysin ( Aero) gene and 9/12( 75%) for Heat labile enterotoxin (Alt ) gene, while none of these isolates were positive for Heat stable enterotoxin (Ast) gene 0/12(0%).The most prevalent genotype was (Aer+ Alt+ Ast_). Antibiogram against 19 antibiotics revealed that, all isolates in showed absolute susceptibility(100%) to Gentamycin, Oflaxacin, Amikacin, Norfloxacin, Imipnem Ciprofloacin. However, multidrug resistance recorded in all isolates, 2 isolates (16.7%) were resistant to eight antibiotics including , Clindamycin, Cephalothin , Vancomycin, Ticacillin-clavulnoc acid, Ceftazidime, Cefoxitin, Trimethoprim-sulfamethaxazon, Azithromycin ,with multidrug resistance index (0.42) , and 5/12 (41.7%) were resistance to seven antibiotics (58.4%) with MDRI( 0.36), four isolates( 33.4%) were resistant to six antibiotics with MDRI ( 0.31), while only one isolates 1/12(8.4%) were resistant to five antibiotic with MDRI (0.26). The present study showed that detection of pathogenic A.hydrophila harboring important virulence genes Aerolysin and Alt with resistance to many clinically important antibiotics is a good indication that chicken feces constitute important source for pathogenic Ahydrophila infecting human that come in contact with chicken and spread of multidrug resistance organisms in environment .


Article
Antibiogram Profiles of Bacterial Isolates from Intensive Care Units in Mosul Teaching Hospitals
أنماط التحسس للمضادات الحيوية للجراثيم المعزولة من وحدات العناية المركزة في مستشفيات الموصل التعليمية

Authors: Zainab A. Al-Jawad زينب عبد الاله الجوادي --- Haitham M. Al-Habib هيثم محمد خليل الحبيب
Journal: Rafidain journal of science مجلة علوم الرافدين ISSN: 16089391 Year: 2012 Volume: 23 Issue: 1E Pages: 52-59
Publisher: Mosul University جامعة الموصل

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Abstract

The study was conducted to identify the types and frequencies of bacterial isolates from patients with Intensive Care Units-acquired infections, and to determine their antibiogram profile. One hundred and fifty four clinical samples were collected from 101 patients who developed clinical suggestion of new infections of urinary tract, lower respiratory tract or wound following the 48 hours of their admission to the Intensive Care Units. All samples were cultured on different culture media, and the isolated microorganisms were identified by the conventional bacteriological methods. The antibiogram profile of selected antibacterial agents was tested. The total number of the bacterial isolates was 69 miroorganisms. Gram-negative bacteria were more frequently encountered (82.6%) than the Gram-positive ones(17.4%) among all samples. The predominant Gram-negative isolate was E. coli (23.2%), followed by Pseudomonas spp. (21.7%), K. pneumoniae (14.5%), Proteus spp. (11.6%)., Acinetobacter spp. (8.7%), and E.aerogenes (2.9%). The predominant Gram-positive bacterial isolate was S.aureus (7.2%), followed by E. faecalis (5.8%), and S.epidermidis (4.3%). The most effective antimicrobial agents were amikacin, ciprofloxacin, vancomycin, gentamicin, chloramphenicol, and azithromycin.

أجريت هذه الدراسة لتحديد أنواع و تكرار الجراثيم المعزولة من المرضى الذين يعانون من أخماج مكتسبة في وحدات العناية المركزة مع فحص حساسيتها للمضادات الحيوية. إذ تم جمع 154عينة من 101 مريض أشتبه بأن لديهم اخماج في الجهاز البولي او الجهاز التنفسي او الجروح بعد مرور 48 ساعة على رقودهم في وحدات العناية المركزة. زرعت العينات على أوساط زرعية مختلفة وتم تشخيصها باستخدام الفحوصات البكتريولوجية التقليدية. وقد تم اختبار حساسية الجراثيم المعزولة لعدد من أنواع المضادات الحيوية. أظهرت النتائج إن العدد الكلي للجراثيم المعزولة هو 69 جرثومة. و قد كانت الجراثيم السالبة لصبغة كرام (82.6%) الأكثر تكرارا من الجراثيم الموجبة لصبغة كرام (17.4%) من بين جميع العينات. كانت جرثومة E.coli هي الأكثر شيوعا من بين الجراثيم السالبة لصبغة كرام (2. 23%)، تلتها Pseudomonas spp. (7. 21%)، K. pneumoniae(14.5%)، Proteus spp.(11.6%)،Acinetobacter spp. (8.7%) و E.aerogenes (2.9%). وكانت جرثومة S.aureus (7.2%) هي الأكثر شيوعا من بين الجراثيم الموجبة لصبغة كرام، تبعتها E.faecalis (5.8%) و S.epidermidis (4.3%). أظهرت الدراسة أن المضادات الحيوية الأكثر فعالية هي اميكاسين وسيبروفلوكساسين و فانكومايسين وجينتامايسين وكلورامفينيكول وازيثرومايسين.

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