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Article
Toxoplasmosis: Serious disease during pregnancy
داء القطط: مرض خطير عند النساء الحوامل

Authors: Majida J.Drueish ماجده جمعه درويش --- Fitua M.Aziz فتوة منور عزيز
Journal: Baghdad Science Journal مجلة بغداد للعلوم ISSN: 20788665 24117986 Year: 2011 Volume: 8 Issue: 1 Pages: 91-95
Publisher: Baghdad University جامعة بغداد

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Abstract

Toxoplasmosis is an infection caused by Toxoplasma gondii that leads to abortion or hydrocephalus during pregnancy.One hundered and twenty two aborted women were selected for this study. Serum samples were collected form Al-Kadhmia and Kamal Al-Samari Hospitals,and laboratories around Baghdad, and tested for specific IgG and IgM anti-toxoplasma antibodies to confirm toxoplasmosis in those women by using ELISA test.The result recorded that51(41.8%) women had antibodies against Toxoplasma gondii,25(59.5%) women were positive for IgG, and 17(40.5%) women were positive forIgM, while 9(17.6%)women were positive for both.

المقدمة:-أن داء القطط اصابه يسببها الطفيليToxoplasma gondiiوالذي يؤدي للاجهاض أو موه الرأس (hydrocephalus)خلال فترة الحمل.الغرض من هذا البحث :- هو لدراسة وجود الطفيليToxoplasma gondiiبين النساء ولمعرفة علاقته بالاجهاض عند النساء الحوامل. كذلك لدراسة علاقة الاصابة القديمه بالاجهاض.الطريقه والمواد:- لقد تم دراسة وجود الاجسام المضادةIgGوIgMعند 172 امرأة،بينهم 50 امرأة سليمه و 122 امرأة تعرضت للاجهاض،حيث تم فحص عينات المصل من خلال اجراءفحص ELISA.النتائج:- لقد وجد ان 12امرأه سليمه تحتوي على IgG ومن مجموع 122 امرأه تعرضت للاجهاض كانت 51 امرأه تمتلك الاجسام المضادة للطفيليi Toxoplasma gondi حيث كانت 25 امرأه مصابه تمتلك الاجسلم المضادة IgG و17 مصابه تمتلك الاجسام المضادة IgMو 9 مصابات تمتلك الاجسام المضادة IgGو IgM .الاستنتاج:- من المعروف ان داء القطط هو مرض خطير خلال فترة الحمل،فقد اشارت لهذا نتائج البحث من خلال وجود الطفيلي المسبب لهذا المرض بين النساء الحوامل والذي يؤدي الى الاجهاض ، كذلك أن وجود الاجسام المضادةIgG والتي تعني اصابه قديمه وتؤدي هذه الاصابه الى الاجهاض ايضا.


Article
Spectroscopic studies of AFP, Anti AFP antibody and AFP/125I- anti AFP antibody complex.
دراسات طيفية لجزيئة البروتين الجنيني الفا (AFP) جزيئة الضد لـ (AFP) والمعقد بين AFP وجزيئة الضد المعلم باليود المشع 125

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Abstract

The aim of this work is to characterize spectrophotometrically the isolated Alpha fetoprotein from human colorectal tumor homogenates and the molecules of both AFP antibody and the complex of AFP/anti AFP antibody.Gel filtration technique was used to separate 125I-anti AFP antibody bound to human AFP from unbound (free) 125I-anti AFP antibody. The characterization of human-AFP, anti-AFP, and (AFP/Anti-AFP) complex were carried out through the ultraviolet (U.V) spectroscopic studies.Factors affecting the light absorption properties of the molecules under investigation in this work such as pH, solvent polarity (solvent perturbation technique), spectrophotometric pH titration and thermal stability have been studied.The spectrophotometric pH titration for h-AFP, anti AFP, and (AFP/anti-AFP) complex showed that pKa for tyrosine was 9.5, 10.2, and 9.9, while for histidine was 5.7, 6.0, and 5.9 respectively. Spectrophotometric pH titration and several spectral changes were obtained in the presence of different polar and non-polar solvents, like the alteration of max position and intensities of protein spectrum, and the appearance of new chromophores on the surface of protein molecule. These chromophores where embedded in an interior region of the protein in the absence of the solvent.The difference in pH and polarity of the solvents is very important thing to characterize the protein molecules spectrophotometrially because they change the positions and values of molecules λmax in the UV region.

الهدف من هذه الدراسة هو للتوصيف الطيفي لجزيئة البروتين الجنيني الفا (AFP) المنقى من متجانسات اورام القولون والمستقيم البشرية وكذلك توصيف مستضد الـ (AFP) والمعقد المتكون من الجزيئة والمستضد. استخدمت تقنية الترشيح الهلامي لفصل المعقد عن المستضد المعلم باليود المشع 125. تم التوصيف الطيفي في منطقة الاشعة فوق البنفسجية وتم دراسة المؤثرات على امتصاص الضوء وهي الدالة الحامضية وقطبية المذيب ( اي تقنية التشويه بالمذيب) والتسحيح الطيفي والاستقرارية الحرارية بوجود تراكيز مختلفة من ملح (NaCl).اظهر التسحيح الطيفي ان قيم ثوابت التاين لمتخلفات الحامض الاميني التايروسين لـ (AFP) و المستضد والمعقد كانت 9.5 و 10.2 و 9.9 على الترتيب بينما كانت للهستيدين 5.7 و 6.0 و 5.9 على الترتيب.تم ملاحظة التغيرات الطيفية الحاصلة على الجزيئات عند استخدام مذيبات مختلفة اذ تغيرت قيم قمم الامتصاص العظمى (λmax)وكذلك مواقع القمم. كما تم ظهور مجموعات قابلة للامتصاص ( الكروموفورات) نتيجة للاختلاف في قطبية المذيبات ونتيجة للتسحسح الطيفي دلالة على ان الكروموفورات كانت ضمن المناطق الداخلية للبروتين وبتاثير المذيب وصلت للسطح واصبحت معرضة للشعاع الطيفي. الاختلاف في قيم (pH)والقطبية عوامل مهمة جدا لتوصيف جزيئة البروتين طيفيا لما لها من تاثيرات على قيم حزم الامتصاص ومواقعها ضمن منطقة الاشعة فوق البنفسجية.


Article
Preparation of Laboratory Kit for Lipoteichoic acid as One of the Constituents of Gram-Positive Bacterial Cell Wall
تحضير عدة الفحص Kit مختبريا لحامض التيكويك الدهني Lipoteichoic acid، احد مكونات جدار الجراثيم الموجبة لصبغة كرام

Authors: Amera M. M. Al-Rawi أميرة محمود محمد الراوي --- Tuka M. Al-Sawaf تُقى مؤيد بكر الصواف
Journal: Rafidain journal of science مجلة علوم الرافدين ISSN: 16089391 Year: 2012 Volume: 23 Issue: 1A Pages: 1-10
Publisher: Mosul University جامعة الموصل

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Abstract

The study includes the production of antibody for LTA in the rabbit after isolation and purefication from S.aureus and St.viridans by injecting the animals regullary and consecutively with pure LTA. Kit was prepared to investigate the presence of antibodies in sera of injected animals by ELISA, the absorbance of the test samples for each of S.aureus - LTA and St.viridans - LTA were 1.0700 ± 0.0141 and 1.0100 ± 0.0141 respectively, The absorbance of control sample, which represent sera of lab animals injected with distilled water was 0.9300 ± 0.0141.

تضمنت الدراسة إنتاج الأجسام المضادة لـحامض التيكويك الدهني LTA في الأرانب بعد ان تم عزله وتنقيته من جرثومتي S.aureus و St.viridans ، وذلك بحقنها بجرعات منتظمة ومتتالية من معلق LTA النقي بكميات متزايدة تدريجيا من المعلق المحضر وحضرت عدة فحص Kit مختبريا للتحري عن وجود الأجسام المضادة في أمصال الأرانب المحقونة به بتقنية الاليزا ELISA إذ بلغت امتصاصية عينات الاختبار لكل من S.aureus - LTA وSt.viridans – LTA (0.0141 ± 1.0700 و 0.0141 ± 1.0100) على التوالي كما بلغت قيمة امتصاصية عينات السيطرة التي تمثل أمصال الحيوانات المختبرية المحقونة بالماء المقطر (0.0141 ± 0.9300).


Article
Prevalence of Anti-Rh (D) Antibody in Karbala

Author: Mohammed Shnain Ali
Journal: Karbala Journal of Medicine مجلة كربلاء الطبية ISSN: 19905483 Year: 2013 Volume: 6 Issue: 2 Pages: 1644-1647
Publisher: Kerbala University جامعة كربلاء

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background: Neither Rh(D) positive individuals nor Rh (D) negative individualsnormally have anti-Rh(D) antibody in their blood; however, Rh (D) negativeindividuals have the ability to form such antibody when they are transfused withRh(D) positive blood.Objective: To determine the prevalence of anti-Rh (D) antibody in Karbala.Patients and methods: From January 2012 to March 2013, 226 patients (127 males and 99females with age range of 20-49 years) were studied after their consent. For each patient, 4ml of venous blood sample was obtained and investigated as follows:Two ml of blood were transferred to EDTA tube for ABO and Rh(D) grouping using thecommercially available kit (monoclonal anti-A, anti-B and anti-D) by doing the ordinary tilemethod (equal volumes of blood and reagent were mixed on tile looking visually for theagglutination for positive reaction and positive result. Absence of agglutination meansnegative reaction and negative result). The other 2 ml of blood were transferred to plain tube,centrifuged and serum is investigated for anti-Rh(D) antibody in Rh(D) negative individualsby double dilutions of each serum and antibody titration method.Results: Out of 226 individuals, 186 (82.3%) were Rh(D) positive, 40 (17.7%) were Rh(D)negative, and 2 were found to be positive for anti-Rh(D) antibody. The prevalence of anti-Rh(D) antibody is 0.88%.Conclusion: The study showed that the prevalence of anti-Rh(D) antibody in Karbala is0.88%.


Article
Antibody response in Hamsters Immunized against experimental Leishmaniasis
استجابة الأجسام المضاده في حيوانات الهامستر الممنعة ضد الخمج بداء اللشمانيات التجريبي

Authors: Harith S. Al-Warid حارث سعيد الورد --- Fawzia A. Al-Shanawi فوزية احمد الشنوي
Journal: Baghdad Science Journal مجلة بغداد للعلوم ISSN: 20788665 24117986 Year: 2011 Volume: 8 Issue: 1عدد خاص بمؤتمر علوم الحياة Pages: 494-499
Publisher: Baghdad University جامعة بغداد

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Abstract

Direct agglutination test was used to evaluate the immunogenicity of three different antigens inoculated in hamsters as one , two and three doses which were: Group (1) inoculated with autoclaved killed Leishmania tropica , Group (2) inoculated with BCG vaccine alone while Group (3) inoculated with mixed antigens (autoclaved killed Leishmania "AKL"+ BCG).(4) Control animals inoculated with phosphate buffer saline.The maximum level of antibody titers were evaluated in animal inoculated with one , two or three dose of mixed antigens (320 , 640 and 1280) respectively when it compared with animals inoculated with corresponding doses of AKL antigen (80 , 160 and 320) respectively. While the minimum level of antibody titers were observed in animal inoculated with two and three dose of BCG (20 and 40) respectively.Our findings suggest that administration of BCG with AKL could lead to a potentially associated antibody response in animals, as well as, such response may evaluate the immunogenicity of some antigens.

اختبرت في هذه الدراسة القابلية التمنيعية لثلاث مستضدات مختلفة باستخدام فحص التلازن المباشر في اربعة مجاميع من حيوانات الهامستر الذهبي التي حقنت بجرعة وجرعتين وثلاث جرع من هذه المستضدات وكما يلي : المجموعة الأولى حقنت بمستضد طفيلي اللشمانيا المقتولة بحرارة المؤصده , المجموعة الثانية حقنت بمستضد لقاح الـBCG , المجموعة الثالثة حقنت بخليط المستضدين السابقين اما المجموعة الرابعة فقد حقنت بمحلول دارئ الفوسفات الملحي واعتبرت مجموعة سيطرة.اظهرت النتائج ان اعلى معيار للأضداد كان في مجموعة الحيوانات المحقونه بجرعة , جرعتين وثلاث جرع من خليط المستضدين (460,320 و1280 على التوالي), اذا ماقورنت بالحيوانات المحقونه بجرعه , جرعتين وثلاث جرع من مستضد طفيلي اللشمانيا المقتولة بحرارة المؤصده(80, 160و 320 على التوالي (, بينما كان اقل معيار للأجسام المضاده في الحيوانات المحقونة بجرعتين وثلاث جرع من مستضد لقاح الـBCG (40,20) على التوالي , ومن خلال هذه الدراسة نستطيع القول بان مستضد لقاح الـBCG يمكن ان يحفز استجابة الأضداد النوعية , كما يمكن اعتبار استجابة الأضداد مؤشرا جيدا لأختبار القابلية التمنيعية لمستضدات معينة.


Article
“Cytomegalovirus Seroprevelance in Iraqi Pregnant Women”

Authors: Sajed Nader --- Mohammed Ayad**
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2012 Volume: 11 Issue: 3 Pages: 303-307
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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ABSTRACT:BACKGROUND:Cytomegalovirus is the commonest cause of congenital viral infection in the developed and developing countries. It is a symptomatic in 90% of infected females. Forty percent of pregnant females transmit the virus to their fetus. Ten percent of born infants whom gain the virus will get the clinical signs plus its neurological sequelae.OBJECTIVE:To outline the relationship between Cytomegalovirus infection among pregnant women and its influence upon their pregnancy outcome.PATIENTS AND METHODS:A case-control study was carried out was carried out in the teaching laboratories – medical city, Baghdad through a period from June 2010 till March 2011; upon 165 pregnant women whom taken as a patient group. Blood samples were taken from them and Cytomegalovirus-IgM antibodies plus Cytomegalovirus-IgG antibodies levels were measured via Elisa technique in both to evaluate the viral infection if present or not.RESULTS:The usual age whom attended the gynea-obestetric clinics were the age group from 11-19 years old 73 cases (44.24%); Next came the age group from 20-29 years old 66 cases (40.00%), thirdly was the age group from 30-39 years old 20 cases (12.12%), Lastly was the age group from 40-49 years old 6 cases (3.64%). The concentration of CMV-IgG among females whom attended clinics were seropositive in nearly half the included cases 66/165 (40.0%); while the CMV-IgM concentration was within the seronegative limits. Secondly came another group of patients with seronegative limits regarding both the CMV-IgM and CMV-IgG antibodies 56/165 cases (33.94%), next 30/165 cases (18.18%) were seropositive in their results pointing to both CMV-IgM and IgG limits; and this might be a middle point distance between the previous group and the last group were the CMV-IgM was positive and the CMV-IgG concentration was negative 13/165 cases (7.88%).CONCLUSION:Cytomegalovirus-IgG antibodies got important role as a protective agent against gestation abortion, if Cytomegalovirus-IgM antibodies are seropositive alone this is a risky factor to the pregnancy outcome


Article
Comparative study of the Antibody Responses to Streptococcus pyogenes between school Children carriers and patients with Tonsillitis
دراسة مقارنة لاستجابة الاجسام المضادة ضد بكتريا المكورات المسبحية (Streptococcus pyogenes) بين اطفال المدارس الحاملين للبكتريا المصابين بالتهاب اللوزتين.

Authors: Fadwa .E. Abd Al-Kareem فدوى عماد عبد الكريم --- Aruba .KH. Abbas عروبة خالد عباس --- Median .A. Hussein مدين علي حسين
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2014 Volume: 55 Issue: 2 Pages: 403-410
Publisher: Baghdad University جامعة بغداد

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This study is designed to evaluate the immune status of patients and schoolchildren with respect to Streptococcus pyogenes. A prospective study was conducted to investigate antibody against GAS in children patients and asymptomatic healthy carriers in serum samples with tonsillitis and compare antibody response (ASO) between patients and healthy carriers with tonsillitis. Tonsillar swabs were obtained to detect the presence of GAS and blood samples were collected to determine elevated ASO titer in serum. A total of 376 sample patients and asymptomatic healthy carriers were included in this study, 142 (37.7%) samples are GABHS positive, included 80 (56.3%) patients and 62 (43.6%) asymptomatic healthy carriers. The finding of a significant relationship between ASO positive and GABHS carriage and patients indicated that ASOT measurement might be used together with throat culture to identify GABHS carriers and GABHS patients. The value of ASOT varies with the age of the subject, and the most pronounced differences are between the values of preschool age children and school age children. The upper limits of normal values for these groups were as follows: preschool age (100) and school age (170). Recently infected children showed raised ASO antibody titer than the carrier. But this study present elevated of ASOT in asymptomatic healthy carriers indicated recent infection or past infection then this children recovery from disease but remain record titer of antibody.

تم تصميم هذه الدراسة لتقييم الحالة المناعية للمرضى وأطفال المدارس فيما يتعلق ببكترياالعقدية المقيحة. وقد أجريت دراسة للتحقيق من الأجسام المضادة ضد بكتريا المكورات المسبحية المجموعة (أ) في الأطفال المرضى والحاملين للبكتريا في عينات مصل الدم مع التهاب اللوزتين ومقارنة بين استجابة الأجسام المضادة (ASO) بين المرضى والحاملين الصحيين مع التهاب اللوزتين. تم الحصول على مسحات من اللوزتين للكشف عن وجود GABHS وعينات الدم تم جمعها لتحديد ارتفاع ASO عيار في مصل الدم. عينة الدراسة تتضمن 376 عينة من المرضى والحاملين للبكتريا صحيين في هذه الدراسة، 142 (37.7٪) كانت ايجابية GABHS ، وشملت 80 (56.3٪) من المرضى و 62 (43.6٪) حاملين للبكتريا غير عرضيين والباقي من العينة هم سلبية للبكتريا. أشارت النتائج وجود علاقة ذات دلالة إحصائية بين ASO و.GABHS وأن قياس ASOT يمكن استخدامها مع زراعة مسحة اللوزتين لتحديد الحاملين GABHS من المرضىGABHS. قيمة ASOT يختلف مع عمر الاطفال، والاختلافات أكثر وضوحا ما بين القيم من الأطفال في سن ما قبل المدرسة والأطفال في سن المدرسة. وكانت الحدود العليا من القيم العادية لهذه المجموعات كما يلي: سن ما قبل المدرسة (100) وسن المدرسة (170). أظهرت الاصابات المتكررة من الاطفال ارتفاع ASO عيار الأضداد في المصابين عن الحاملين. ولكن في هذه الدراسة الحالية ارتفاع ASOT في الحاملين للبكتريا نتيجة عدوى أخيرة أو عدوى في الماضي ثم شفاء الأطفال من المرض ولكن تبقى عيارة من الأجسام المضادة مسجلة.


Article
The Effect of Silver Nanoparticles on Cellular and Humoral Immunity of Mice in Vivo and in Vitro

Author: Rand M. Abd AL-Rhman ,ShaimR.Ibraheem, Israa AL-Ogaidi
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2016 Volume: 15 Issue: 2 Pages: 21-29
Publisher: Baghdad University جامعة بغداد

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Abstract:This study investigated the immunological adjuvant effect of silver nanoparticles AgNPs which have been prepared and characterized in previous study. The silver nanoparticaleswere synthesized by biological method using green (G) and black (B) tea as reducing agent. AgNPs were 50nm and 30 nm respectively with like spherical in shape. The effect ofAgNPs as adjuvant were tested both in vitro and in vivo. The effect of the concentration2mg/kg ofAgNPs was evaluated in mice by intraperitoneal immunization. In vitro the number of both T and B cells, monocytes and neutrophils populations showed an increase in absolute cell number in 2B and 3G groups in the immunized mice compared to the control group, whereas the relative cell numbers remained constant in eosinophils and basophils between group2 and control. The levels of IL 4 and IL6 showed significant elevation (P≥ 0.05) in the serum groups (2B,3G) of animals which were (23.1 ± 0.80, 32.4 ±1.35) pg/ml for group2 (36.7 ±1.76and 50.5 ± 1.76) pg/ml for group3 compared to those that found in control (12.10 ±.23and 19.5±0.34) pg/ml respectively.So,the inoculation with AgNPs, showed no signification difference at (P ≥ 0.05) in group2B while the concentration of IgGgroup3G showed significant elevation which were (4.9±0.11 and 6.8±0.12) mg/ml respectively as compared with control group (4. ±50.11) mg/ml.Also the results showedan increase in phagocytes of treated mice's blood 36 (32%)and 95 (45%) for group2B and group3G respectively as compared with control 4 (17%). Therefore,we conclude that AgNPs have a significant adjuvant effect and the mechanism of this effect is mainly ascribed to the recruitment and activation of local leukocytes, especially lymphocytes, increase cytokines levels in mice, IgG concentration and phagocytes. The humoral and cellular immunities were significantly enhanced in immunized mice, whichcan be utilized as an effective adjuvant to improve the immunoprotection, the result is beneficial for the future applications, especially in biomedicine.


Article
A Study Of Angiogenesis In Human Colorectal Tumors By Using Anti-Cd34 Antibody

Author: Nabil Salmo
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2007 Volume: 49 Issue: 4 Pages: 425-433
Publisher: Baghdad University جامعة بغداد

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Background: Angiogenesis plays a crucial role in tumorigensis; several reports have described a significant increase in microvessel density (MVD) in colorectal carcinogenesis There are several methods to measure the angiogenesis in neoplasms, but immunohitochemistry seems to be the mainstay of all. This method enable us to measure the tumor microvessel densities highlighted by using antibodies directed against endothelial cell markers like CD31,CD34 or others; then assessment of MVD by manual count of the number of microvessels in what appears to be the most vascular area of the tumor(called the hot spot) using a protocol described by Weidner et al.Automated cellular imaging system is used to analyze immunohistochemically stained slides .studies have shown that the device offers accurate precision and reproducibility of immunostained slide analysis exceeding that possible with manual evaluation which was the prevalent method . Aims of the study: To assess the angiogenesis in normal, adenomatous (benign) and malignant colorectal tissues using CD34 and the microvessels will be measured both manually by hot spot method as the MVD and by the use of computerized image analysis system as fraction area ,we correlate between microvessels density and fraction area with various clinicopathological parameters in colorectal cancer (CRC), and to a compare between the results which obtained from both methods. Methods: Paraffin embedded archival materials from 50 cases including three normal resection (non tumoruos) margins, 12 benign colonic lesions and 35 colonic adenocarcinoma were used . 5mm section were cut and they were stained by anti CD34 antibody Angiogenesis was measured as MVD by two methods : manually by light microscope and by a computer image analysis system (as fraction area). Then the MVD and fraction area were correlated with different clinicopathological parameters. Results : This study demonstrate that there is a statistical difference in MVD and fraction area in both hot spot method and CIAS respectively between benign and malignant tumors . P value < 0.05 in hot spot method and less than 0.001 in CIAS and there was highly significant correlation between MVD and fraction area with the grade . There was significant increase in MVD and fraction area from well differentiated to moderately differentiated and to poorly differentiated .There was no significant correlation between MVD and lymph node involvement by hot spot method but CIAS proved a significant correlation between fraction area and lymph node involvement.Both methods (hot spot and CIAS) proved no significant correlation with age , sex , size of the tumor , site of the tumor , stage of the tumor and the number of lymph node involverment . Conclusions: Assessment of tumor vascularity by counting the microvessels using anti CD34 antibody is useful in quantifying angiogenesis in colorectal adenocarcinoma . Intratumoral microvessels count (by manual and computerized method) is important in the assessment of the biological behavior of CRC. Microvessel count is higher in malignant tumors than benign tumors and it is correlated with the tumor grade and higher MVD is associated with lymph node metastasis . Statistically no significant correlation was found between MVD and age, sex of the patients, tumor size, site, stage, histological type and number of lymph node metastasis . The use of CIAS for assessment of angiogenesis is reliable, reproducible and more precise than the manual method.


Article
The association of anticardiolipin antibody IgM with first trimester recurrent abortions
العلاقة بين الجسم المضاد للدهون من نوع آي جي آم والإسقاطات المتكررة خلال أول ثلاثة اشهر من الحمل

Author: Dr. Mohammad Shnain Ali
Journal: KUFA MEDICAL JOURNAL مجلة الكوفة الطبية ISSN: 1993517X Year: 2010 Volume: 13 Issue: 2 Pages: 157-161
Publisher: University of Kufa جامعة الكوفة

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Abstract

AbstractBackground: Anticardiolipin antibody has a clearly documented association with recurrent abortions in patients with autoimmune diseases; however, the association of Anticardiolipin antibody with first trimester abortions is not clearly documented in patients with no evident autoimmune diseases.Aim: To estimate the prevalence of Anticardiolipin antibody IgM in patients with first trimester recurrent abortions and to find if there is any association between them.Materials and methods: A total number of 90 women were included, 45 had history of recurrent abortions(more than 2 abortions) and 45 were healthy control women with one or more than one delivery and no history of abortions. Blood samples from the 90 women were taken for investigations including blood group, blood sugar, blood urea, complete blood picture, ESR, general urine examination, TORCH test, VDRL test and Anticardiolipin antibody IgM by ELISA technique in which levels higher than 15 MCL unit are considered to be positive, while levels below 15 MCL unit are considered to be negative.Results: Out of 45 patients, 24.4%(n=11) had history of explained recurrent abortions, 9 of them due to toxoplasmosis and 2 of them were due to cytomegaloviral infection. while 75.6%(n=34) had no explained causes. Significantly raised levels of anticardiolipin antibody IgM was present in 11 out of 45 patients i.e 24.4% of patients while IgM levels were normal in 34 out of 45 patients i.e 75.6%. The total number of explained recurrent abortions was 11 (8 out of 11 had significantly high IgM antibody i.e 72.7% while 3 out of 11 had normal IgM levels i.e 27.3%). The total number of unexplained recurrent abortions was 34 ( 3 out of 34 had significantly high anticardiolipin antibody IgM i.e 8.8% while 31 out of 34 patients had normal levels i.e 91.2%) while the antibody was negative (normal) in the control group. Patients with significantly high levels of anticardiolipin antibody who had history of explained recurrent abortions are eight. Seven of them i.e 87.5% had positive antitoxoplasma antibody IgM and one of them i.e 12.5% had positive anticytomegalovirus antibody IgM. Conclusion: Anticardiolipin antibody IgM is not associated with first trimester recurrent abortions.

الخلاصةالخلفية: للجسم المضاد للدهون علاقة مثبتة مع الاسقاطات المتكررة في حالة وجود الامراض المناعية المكتسبة مع ذلك فان العلاقة غير مثبتة في حالة عدم وجود الامراض المناعية المكتسبة. الغرض: معرفة وجود او عدم وجود علاقة بين الجسم المضاد للدهون من نوع آي جي ام والاسقاطات المتكررة خلال اول ثلاثة اشهر من الحمل.المواد والطرق: تمت دراسة 90 حالة(45 من النساء الحوامل ممن لهم اكثر من حالتين من الاسقاطات اول ثلاثة اشهر من الحمل و 45 من النساء ممن لهم اكثر من ولادة طبيعية وليس لديهم أي حالة اسقاط كمجموعة تتمتع بالصحة). تم جمع عينات الدم والادرار لاجراء التحليلات المرضية التالية: فصيلة الدم,نسبة السكر في الدم, يوريا الدم,صورة الدم الكاملة,نسبة ترسيب الكريات الحمراء,تحليل الادرار العام, اختبار تورج, اختبار السفلس, والجسم المضاد للدهون من نوع آي جي ام بطريقة اليزة وتم اعتبار المستوى الاعلى من 15 وحدة ايجابيا والمستوى الاقل او يساوي 15 سلبيا. النتائج: وجدت نسبة 24.4%(عدد 11) ممن لديهم اسقاطات متكررة مفسرة(9 منهم بسبب داء القطط و 2 منهم بسبب السايتوميجالوفايروس)بينما نسبة 75,6%(عدد 34) ممن ليس لديهم اسباب لتفسير الاسقاطات المتكررة. وجد ان مستويات الجسم المضاد للدهون من نوع آي جي ام بطريقة اليزة عالية احصائيا لدى 11 من مجموع 45 مريضة أي 24.4%(8 مرضى ممن لديهم اسقاطات متكررة أي 72.8% و 3 مرضى فقط ممن لديهم اسقاطات غير مفسرة الاسباب أي 8.8%)الاستنتاج: لا توجد علاقة بين الجسم المضاد للدهون من نوع آي جي ام والاسقاطات المتكررة خلال اول ثلاثة اشهر من الحمل في حالة عدم الاصابة بالامراض المناعية.

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