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Article
Detection of siRNA-Directed DNA Methylation and Gene Silencing in Response to Salinity Stress

Author: Hatem K. Abbas, Mike Wilkinson, Penny Tricker
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2017 Volume: 16 Issue: 2 Pages: 89-110
Publisher: Baghdad University جامعة بغداد

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Abstract

Small RNAs are able to organise a lot of biological machineries in organisms. Different types of sRNAs play divergent roles in the regulation of gene expression in plants, including RNA-directed DNA methylation (RdDM), which results from double-stranded RNAs and is often capable of directing DNA cytosine methylation at target sequences. Cytosine methylation forms the basis of an epigenetic regulatory system that has been shown to be involved in abiotic and biotic stress responses of plants. In this study applied a direct delivery method to introduce SOS1-siRNA (small interfering RNA) and HKT1-siRNA into Arabidopsis thaliana to test their ability to direct DNA methylation within promoters of the AtSOS1 and AtHKT1 genes in response to salt stress during the growth period. There was clear evidence of the siRNAs’ uptake and that the AtSOS1 promoter was methylated in the presence of HKT1-siRNA when treated with salt. In addition, lethal effects were observed on the phenotype, which suggested that AtSOS1 and AtHKT1 genes’ expressions had been affected. Direct siRNA delivery was therefore used successfully and it caused significant effects on the phenotype of plants. This method has the potential to allow targeted regulation of gene expression in response to stress.


Article
Study the Effect of Arabidopsis thaliana Extract on Reducing Blood Glucose Level in Diabetic White Albino Mice
دراسة تأثير مستخلص نبات اذان الفار Arabidopsis thaliana في خفض مستوى الكلوكوز في دم الفئران البيضاء المصابة بالسكر

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Abstract

This study was designed to evaluate the effect of aqueous extract of Arabidopsis thaliana seeds on reducing glucose level for white albino mice. Twenty adults mice were used, divided randomly into four groups (five mice per each group). The first group (normal mice) was administrated with 0.1 ml of distilled water as a control, the second group (normal mice) was administrated with 0.1 ml of the plant extract, whereas the third and fourth groups (diabetic mice) were administrated with single dose of alloxan (150 mg/kg of the body weight) to induce diabetes, and the fourth group was administrated with 0.1 ml of the plant extract for 10 days, then blood glucose level was measured for all of the experimental animals (diabetic and non diabetic). Results showed clear increasing in glucose levels in the diabetic mice, while significant reduction was recorded in glucose levels of the normal mice that was treated with the plant extract as compared with the control group. These results indicate that Arabidopsis thaliana seeds aqueous extract possesses a hypoglycemic effect.

صممت هذه الدراسة لتقييم تأثير المستخلص المائي لبذور نبات اذان الفار Arabidopsis thaliana على مستوى السكر في دم الفئران البيضاء. استعملت في هذه التجربة 20 فأرة في مرحلة النضوج, قسمت عشوائيآ الى اربعة مجاميع (5 فئران لكل مجموعة). جرعت المجموعة الاولى 0,1 مللتر من الماء المقطر, المجموعة الثانية جرعت 0,1 مللتر من مستخلص آذان الفأر ( تركيز200 ملغم/ كغم من وزن الفأر), والمجموعة الثالثة والرابعة حقنت بجرعة مفردة من الالوكسان ( 150 ملغم / كغم .من وزن الفأر ) لأستحداث مرض السكري وقد جرعت المجموعة الرابعة 0,1 مللتر من مستخلص المائي ولمدة 10 ايام. بعدها تم قياس مستوى الكلوكوز بدم الحيوانات المختبرة (المصاية والسليمة). بينت النتائج زيادة واضحة في مستوى الكلوكوز في مجموعة الفئران المعاملة (المصابة), ونقصان واضح في مجموعة الفئران المصابة والتي جرعت بالمستخلص المائي للنبات, وكذلك نقصان في مستوى الكلوكوز للفئران السليمة المجرعة بالمستخلص بالمقارنة مع المجموعة الطبيعية (السيطرة). تشير هذه النتائج الى ان المستخلص المائي لبذور آذان الفار تمتلك تاثير خافض للسكر.

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