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Impact of in vitro Preparation with Citrullus Colocynthis on DNA Normality of Human Asthenozoospermic Semen

Authors: Hind A Abdul-Ghafoor --- Rushdie S AL-Hadithy --- Mohammad 0. Selman --- Saad S. Al-Dujaily
Journal: Iraqi Journal of Embryos and Infertility Researches المجلة العراقية لبحوث الأجنة والعقم ISSN: 22180265 Year: 2015 Volume: 5 Issue: 1 Pages: 36-45
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background:Citrullus colocynthis (CC) is an herbal medicine used in the treatment of a wide range of diseases. Recently, It has been studied on in vitro activation of mice sperms with no research concern the effect of this plant on the DNA normality following in vitro preparation of human semen samples.Objectives: The present study was aimed to examine the effect of adding CC in the medium used for in vitro sperm activation of asthenospermic men and related factors and to found out the effect of CC on the DNA status following the activation.Materials and Methods: Citrullus colocynthis aqueous extract (0.01mg/ml culture medium) was used for in vitro sperm activation . One hundred and ten semen samples were divided into two groups, the first group activated in vitro by adding CC extract to the culture medium Ham's F-12 (the treated group) and, the second group sperm activated in vitro with Ham's F-12 alone ( the control group).Results: In vitro activation of sperms with aqueous extract of CC has shown positive effect on sperm concentration, sperm motility, and grade activity of progressive forward movement. There was a significant (P<0.05) increase in certain sperm function parameters of asthenozoospermic in treated group compared with the control group .The study showed that the DNA fragmentation resulted from treated group were lower than that of control group.Conclusions: According to the results of present study, adding aqueous extract of CC to the human sperms activation culture medium leads to an improvement in certain sperm function parameters of asthenozoospermic men with no harmful effect on the human sperms DNA with decrease in DNA denaturation.


Article
Prevalence of Antisperm Antibodies in Asthenospermic Infertile Male
دور الأجسام المناعية المضادة للحيامن في عقم الرجال

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Abstract

Recently there has been a lot of discussion about the role that the immune system plays in infertility. The immune system generally works to protect the body from foreign cells and bacteria. Sometimes though problems within the immune system prevent it from working. Many couples facing infertility issues may actually have immune system dysfunction which is preventing them from conceiving .luckily new. tests are now available to pinpoint these immune factors in infertility properly. prospective study was carried out in the infertility clinic of Azadi teaching hospital in Kirkuk city from January 2007 to January 2009.The study included 155 male with primary or secondary infertility with abnormal seminal fluid analysis specifically decreased motility of the sperms (asthenospermia), their ages ranged from(18-55)years. The entire patients included in the study have been proved to be infertile as they fail to conceive after a year of regular intercourse without contraception. All patients were subjected to a special questionnaire used for analytical purposes; both blood and the seminal fluid samples of all were examined for presence of antisperm antibodies.the study showed that 75 patients (48.4%) out of 155 patients showed a positive antisperm antibody (ASA ) which is statistically significance (P value 0.04), 55 patients (73.3% ) from those with positive results showed ASA in their serum, while 20 patients (26.6%) of them showed ASA in their seminal fluids which showed is also statistically significance (P value 0.03)in conclusion the study shows that an antisperm antibody has an impact on asthenospermia and infertility.

حدوث الأجسام المناعية ضد الحيامن في الرجال المصابين بالعقم مع بطئ حركة الحيامن لديهم. أجريت هذه الدراسة في عيادة العقم في مستشفى ازادى التعليمي في مدينة كركوك للفترة من كانون الثاني 2007-كانون الثاني 2009, شملت الدراسة 155 رجلا من الذين كانوا يعانون من عقم أولى أو ثانوي وكانت لديهم ضعف وقلة في حركة الحيامن أعمارهم يتراوح بين 18-55 سنة, بعد إجراء الفحص السرير عليهم تم البحث عن وجود الأجسام المناعية للحيامن لديهم في كل من السائل المنوي و الدم وكانت النتائج إن 75 رجلا (%48.8 ) من الذين كانوا يعانون من ضعف في حركة الحيامن ظهرت لديهم أجسام مناعية وان 55 رجلا من 75 (73.3%) منهم ظهرت الأجسام المناعية لديهم في الدم و 20 منهم (26.6%) ظهرت الأجسام المناعية لديهم في السائل المنوي وهذه النتائج مهمة إحصائيا . أظهرت الدراسة أن للأجسام المناعية تأثير على حركة الحيامن وبالتالي على العقم لدى الرجال و توصى بإجراء التحري عن الأجسام المناعية في الظروف المثالية على كافة تحاليل السائل المنوي أما في حال تعذرها فيجب أن يجرى على الأقل على اؤلك الرجال الذين لديهم احتمالية امتلاكهم للأجسام المناعية.

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