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Article
Gallstones associated with bacterial incidence
حصى المرارة المرتبطة بالاخماج الجرثومية

Author: A.Y.Abd-Alkareem عبير يوسف عبد الكريم
Journal: Tikrit Journal of Pure Science مجلة تكريت للعلوم الصرفة ISSN: 18131662 Year: 2011 Volume: 16 Issue: 4 Pages: 19-22
Publisher: Tikrit University جامعة تكريت

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Abstract

Objective: To study the correlation between the gallstones accumulation and bacterial incidence . Patients and methods : This study was carried out for the period July 2009 to February 2010 in Ramadi General hospital in Ramadi City. Forty patients were diagnosed clinically as having gallstones were submitted to surgical operation .Stones were collected for culture, all the information study patients were recorded. Results : 40 stones were collected , male to female ratio was 1:2.8 , age ranged between (30-60) years old. The stones were diagnosed based on the morphology, size and color , cholesterol stones were found in 25(62.5%) and pigment stones in 15(37.5%). 70% appeared no growth of pathogenic microorganism while 30% of these stones show bacterial growth of the following bacteria, Escherichia coli 12(48 (%, Klebsiella pneumoniae 4 (16% ) , Pseudomonas aeruginosa 3 )12 %), Salmonella enterica enterica (S.Typhi) 4 (16%) and Staphylococcus aureus 2 (8%) . Conclusion : It was concluded that bacteria actually constitute a substantial portion of the stone and are more than just a remote source of ß-glucuronidase.

الهدف: دراسة العلاقة بين الخماج الجرثومية وتكوين حصى المرارة.المرضى وطرق العمل: أجريت الدراسة بين تموز2009 إلى شباط 2010في مستشفى الرمادي العام في مدينة الرمادي على 40 مريضا شخصت لديهم حصاة المرارة واخضعوا للعملية الجراحية ،اجري الزرع البكتيري لجميع الحصوات ،سجلت جميع العوامل ذات العلاقة بالمرضى . أ لنتائج:جمعت 40 حصى ،نسبة الرجال الى النساء(2,8:1) وكانت أعمار المرضى تتراوح بين30-60 سنة شخصت نوعين من الحصى بالاعتماد على المظهر الخارجي والحجم واللون هما حصى الكوليستيرول25(62,5%) وحصى الصبغة 15(37,5%) .أوضحت النتائج ان70% من الحصى كانت سالبة للزرع الجرثومي و30% كانت موجبة للزرع الجرثومي.وشملت الأنواع التالية: Escherichia coli 12(48%) , Klebsiella pneumoniae 4(16 %) , S. Typhi 4 (16%), Pseudomonas aeruginosa 3(12%) , Staphylococcus aureus 2 (8%) .الاستنتاج: استنتج من هذه الدراسة إن البكتريا تشكل جزء أساسي في تكوين حصى المرارة وهي أكثر من أن تكون مجرد مصدر محفز لإنزيم β-glucuronidase

Keywords

Gallstones --- Bacteria


Article
SYNTHESIS, CHARACTERIZATION AND STUDY THE EFFECT OF (3,5-DIMETHYL-1H-PYRAZOL-4- YL) MERCURY (II) CHLORIDE ON GROWTH INHIBITION OF SOME BACTERIA ,YEAST AND SOME FUNGI (IN VITRO).
تحضير وتشخيص ودراسة الأثير المثبط للمركب 5,3-ثنائي مثيل -1H-بايروزول-4-يل) كلوريد الزئبق على نمو بعض البكتيريا والخمائر وبعض الفطريات.

Author: Eman.T.Ali أيمان طارق علي
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: Print:18138497 E; 24108456 Year: 2008 Volume: 7 Issue: 2 Pages: 65-73
Publisher: Basrah University جامعة البصرة

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Abstract

The reaction of 3,5-dimethyl-1H-pyrazole with mercury (II)acetate in absolute methanol gave (3,5-dimethyl-1H-pyrazol-4-yl) mercury(II) chloride (DMPMC), which characterized by microanalysis, 1H, and 13C-NMR and IR. It was found that 0.22M of (DMPMC) dissolved in ethanol/water added to Muller-Hinton Agar medium and Sabourauds-Dextrose agar medium (SDA) respectively, inhibited the growth of some gram negative bacteria [Escherichia-coli, Klebsiella-aerogenes and Pseudomonas-aeruginosa], also gram positive bacteria [Staphylococcus-aureus, Streptococcus-pyogenes] and Candida-albicans as well as some fungi [Aspergillus-flavus, Aspergillus-fumigatus and Aspergillus-niger]. Higher concentrations of (DMPMC) solution in to the media inhibited growth of bacteria yeast and fungi under studies more strongly. The minimal inhibitory concentration (MIC)and the cytotoxicity of (DMPMC) were studied against human being blood , it was found that it has no haemolysis at different concentrations in vitro

تضمن البحث زئبقية المركب 5,3- ثنائي مثيل بايروزول بواسطة خلات الزئبق وتم تشخيص المركب باستخدام مطيافية الأشعة تحت الحمراء وطيف الرنين النووي المغناطيسي للبروتون وطيف الرنين النووي المغناطيسي للكاربون-13 بالأضافه إلى التحليل الدقيق للعناصر والتي أكدت صحة الصيغة المقترحة للمركب. حيث وجد إن 0.22M من المركب (DMPMC) المذاب في مزيج من الأيثانول والماء ثبط نمو البكتيريا سالبة الغرام( Pseudomonas aeruginosa E.coli, Klebsiella aerogene) والبكتيريا موجبة الغرام (Staphy.aureus Strept.pyogenes) والخميرة Candida albicans بالأضافه إلى تثبيط نمو بعض الفطريات -Aspergillus flaves, Aspergillus niger, Aspergillus fumigatus كما وجد إن التراكيز العالية من المركب (DMPMC) تؤدي إلى تثبيط النمو بشكل اكبر. تمت دراسة التركيز الأدنى المثبط وكذلك دراسة السمية لدم الإنسان ووجد انه لايوجد تحلل لكريات الدم الحمراء عند تراكيز مختلفة من المركب . وتعتبر هذه الدراسة هي الخطوة الأولى لدراسة هذا المركب الجديد والذي من الممكن الأستفاده منه كدواء في المستقبل.


Article
Prevalence of bacterial infection and their sensitivity in patients undergoing an infertility eval

Author: Wafaa Hazim Saleh وفاء حازم صالح
Journal: Al-Kindy College Medical Journal مجلة كلية الطب الكندي ISSN: 18109543 Year: 2010 Volume: 6 Issue: 1 Pages: 33-38
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Infection with sexually transmitted diseases is broad and includes bacterial, viral and protozoa infection. Large number of infected people goes untreated because of symptomatic or unrecognized infections.Patients and methods: Forty five patients was complaining from infertility (primary or secondary), consulting Kammal El-Sammari Hospital for infertility from May - 2008 to February - 2009. Control group consisted of twenty fertile women that consulting private clinic for checking. Four swabs were taken from each woman in two groups. Two swabs were taken from posterior fornix of the vagina (High vaginal swab) and the last two were taken from endocervical canal. First swab (vagina and cervix) was examined directly under light microscope (wet mount) and stained by Gram stain. The other swab was cultured on Blood and Chocolate agar.Results: The patients group consisted from forty-five female patients, their aged ranged from (22-45 years), (X= 32.9). Direct examination (wet mount) and Gram staining of high vaginal swab showed significant increased in leukocyte (pus cells) and epithelial cells in infertile group than normal one. The isolated bacteria from culture of high vaginal swab were Streptococcus agalactiae (group B streptococci) which was significantly increased than fertile group. This bacteria was sensitive to Cephaloxtin and Cephotaxime and resistant to PenicillinConclusions: The isolated bacteria from culture of high vaginal swab were Streptococcus agalactiae (group B streptococci). This bacteria was sensitive to Cephaloxtin and Cephotaxime and resistant to Penicillin .

Keywords

:Infertility --- bacteria --- antibiotic


Article
Assessment of the Effects of Pregnancy-Induced Hypertension uponPregnant Women and Their Pregnancy Outcome in Erbil City

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Abstract

Objective: To determine the possible role of cockroaches as a transmitter of medically important microorganisms, pathogenic organisms were isolated and identified from external and guts of cockroaches trapped at hospitals and houses. Methods: One hundred and fifty two cockroaches were collected from different parts of hospital and houses around it. They were collected for isolation and identification of bacteria and parasites from external surface and internal digestive tract of cockroaches. Statistical analysis was done by using t- test. Results: This study show that high rates of cockroaches show contamination of medically important bacteria and parasites. The species of the pathogenic agents was determinate and the number and percentages of infected cockroaches was recorded. Conclusion : And for this, cockroaches act as a potential transmitter of medically important pathogenic and may cause of nosocomial infection in both hospitals and houses. Therefore, control of cockroaches will substantially minimize the spread of infectious diseases in our environment.

Keywords

cockroaches --- Bacteria --- Parasites.


Article
Causative Organisms and Risk Factors In Bacterial Meningitis in Al-Elwia Childhood Hospital - Baghdad

Author: Ban Abdul Hameed بان عبد الحميد
Journal: Al-Kindy College Medical Journal مجلة كلية الطب الكندي ISSN: 18109543 Year: 2011 Volume: 7 Issue: 2 Pages: 59-68
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Childhood meningitis is a major cause of morbidity and mortality, Hemophilus influenza b (Hib) is the most common cause in many countries, especially below 5 years and before the development of conjugated Hib vaccine, it is followed by Streptococcus Pneumonia, and then N. meningitides, in addition to other microorganisms. Objective: To identify the causative organisms of bacterial meningitis and to identify the factors predisposing significantly to the incidence of bacterial meningitis. Method: This cross sectional , study was done in Al-Elwia Pediatric Hospital during the period 1st of January 2007 to 30th of June 2007.Eighty four patients with presumptive diagnosis of meningitis were included in this study, from the age of 2 months -12 years, History about some risk factors were taken in details. Analysis of cerebrospinal Fluid (CSF) with Gram stains & cultures were done in all cases. Results: The number of cases of meningitis was 50 (20 proved meningitis & 30 partially treated meningitis), while 34 patients are found to have no meningitis. Streptococcus pneumoniae was identified in 45%, Hemophilus Influenza b in 20%, while Nisseria meningitides 5%, other organisms include Escherichia coli, Staphylococcus aureus, Klebsiella, & salmonella. Many factors affecting the occurrence of bacterial meningitis & these include: age, sex, residence, body weight and home overcrowding. Conclusions: Streptococcus pneumoniae was found to be the predominant microorganism causing bacterial meningitis in children aged 2 months-12 years, followed by Hemophilus influenza b, while N. meningitides were one of the rare bacteria which had been identified. E. coli was found to be the major cause in cases of ventriculo-peritoneal (VP) shunt meningitis.


Article
Effect of Magnetic field on the growth of Escherichia coli and Staphylococcus aureus

Authors: Samir.H.Nasher --- Amal.A.Hussein
Journal: Engineering and Technology Journal مجلة الهندسة والتكنولوجيا ISSN: 16816900 24120758 Year: 2008 Volume: 26 Issue: 4 Pages: 458-461
Publisher: University of Technology الجامعة التكنولوجية

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Abstract

Magnetic fields with two densities (1200 , 3200)gauss experminted on twokinds of bacteria Escherichia coli and Staphylococcus aureus in Nutrient brothmedia . The field subjected for (1,2,3) weeks, and the growth monitored usingoptical density (OD) method.The results shows greatest effects of 3200 gauss than 1200 gauss on bacteriagrowth comparing with control sample after third week. The OD resulte of bacteriaEscherichia coli (0.3) with 3200 gauss and (6) with 1200 gauss comparing withcontrol sample(175.4) and bacteria Staphylococcus aureus (1) with 3200 gauss,and (10) with 1200 gauss comparing with control sample (174.4).

لمعرفة gauss (3200 , تمت تجربة المجال المغناطيسي بشدتين مختلفتين ( 1200Staphylococcus و Escherichia coli تأثيرهما على نمو نوعين من البكتيريا المرضيةوقد اجري النمو في ثلاث فترات مختلفة .Nutrient broth في وسط المرق المغذي aureus. Optical density 3) اسابيع. بعدها فحص النمو بطريقة قياس الكثافة الضوئية ، 1،2)كان اكبر من الشدة 1200 gauss اظهرت النتائج ان تأثير شدة المجال المغناطيسي 3200على نمو نوعي البكتريا والشدتان اثرتا على النمو بصورة كبيرة قياسًا بالسيطرة لاسيما gauss0.3 ) عند )Escherichia coli بعد الاسبوع الثالث حيث وصلت الكثافة الضوئية لنمو بكترياقياسًا بنموذج السيطرة ( 175.4 ) ولبكتريا gauss و( 6) عند الشدة 1200 gauss الشدة 3200والى ( 10 ) مع gauss 1) على التوالي مع شدة المجال 3200 )Staphylococcus aureus.( قياسًا بنموذج السيطرة ( 174.4 gauss الشدة 1200


Article
ISOLATION AND IDENTIFICATION OF MUTAN'S STREPTOCOCCI BACTERIA FROM HUMAN DENTAL PLAQUE SAMPLES

Author: Nada H.A. Al-Mudallal, Essam F.A. Al-Jumaily
Journal: Al-Nahrain Journal of Science مجلة النهرين للعلوم ISSN: (print)26635453,(online)26635461 Year: 2008 Volume: 11 Issue: 3 Pages: 98-105
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Fifty plaque samples were collected from teeth. Forty five samples were considered to be positive bacterial isolates about (104 bacteria/ml) using selective Ms-agar (Mitis-Salivarius agar) medium. Thirty isolates were considered to be related to the genus Streptococcus and specially to the mutans streptococci of various group; S. sobrinus (serotype D, G), S. mutans (serotype C, F), S. cricetus (serotype A) and S. rattus (serotype B) with percentages of (39.29%), (30.30%), (18.18%) and (3.03%), respectively depending on biochemical and Lancefield grouping identification systems.

عزلت خمسين عينة من الطبقة المغطية لسطح السن. خمس وأربعون عينة أظهرت نموا ً إيجابيا ً للبكتريا حوالي ( 410 بكتريا/مل) على سطح الوسط المخصص للنمو (Milis-Salivarius agar medium). ثلاثون عزلة إعتبرت عائدة لبكتريا Streptococcus وخاصة للمكورات الفموية والتي تباينت بين الأنواع S . sobrinus (serotype D,G) و S. mutans (serotype C,F) و S. cricetus (serotype A) و S. rattus (serotype B) وبنسب (39.39%) و(30.30%) و(18.18%) و(3.03%) تباعا ً والتي تم تشخيصها إعتمادا ً على الطرق البايوكيميائية وخصوصية عالق ال (Latex) المضاد والخاص بالمجاميع (A وB وC وD وF وG) المتعددة السكريدات (المستضاد) والموجودة على الجدار الخارجي لهذه البكتريا (Lancefied group) أو (Serotype group).

Keywords

Mutan’s --- streptococci --- bacteria


Article
Isolation of aerobic and anaerobic bacteria from suspected enterotoxaemia cases in lambs
عزل الجراثيم الهوائية و اللاهوائية من حالات التسمم المعوي المحتملة في الحملان

Author: N. S. Mechael نبال سامي ميخائيل
Journal: Iraqi Journal of Veterinary Sciences المجلة العراقية للعلوم البيطرية ISSN: 16073894 Year: 2012 Volume: 26 Issue: 1 Pages: 29-32
Publisher: Mosul University جامعة الموصل

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Abstract

Ninety cases of clinically diagnosed enterotoxemia infection in lambs at AL-Hamdaniya region where studied for isolation of aerobic and anaerobic bacterial causes, faecal samples were collected from all suspected cases during January- June 2008, the results show that 41.6% of the isolates were Cl. perfringens as pure single isolates, while mixed infection of Cl. perfringens with each of Enterococci and staphylococcus in percentage of 26.04%, 20.83% respectively, also mixed infection of Cl. septicum with each of Staphylococcus and E.coli were isolated at the percentage of 5.2%, 6.25% respectively. Highest bacterial isolation was from the faecal samples collected during April. McIntosh jar method show isolation of pure culture of anaerobic bacteria (Cl. perfringens), while Candle jar method show detection of 56 isolates in mixed cultures of aerobic and anaerobic bacteria.

تم دراسة تسعون حالة اصابة بالتسمم المعوي مشخصة سريريا في الحملان في منطقة الحمدانية لعزل الجراثيم الهوائية واللاهوائية المسببة لتلك الاصابات وقد تم جمع نماذج براز من جميع الحالات المشكوك بها خلال الفترة من كانون الثاني لغاية حزيران 2008، اشارت النتائج الى عزل جراثيم لاهوائية (مطثيات بيرفرنجس) بنسبة 41,6% بشكل مفرد، بينما عزلت جراثيم اخرى من اصابات مختلطة من جراثيم مطثيات بيرفرنجس مع كل من جراثيم انتيروكوكس و جراثيم المكورات العنقودية بنسبة 26,04%، 20,83% على التوالي، وجراثيم المطثيات الانتانية مع كل من جراثيم المكورات العنقودية وجراثيم الاشيريكيا القولونية بنسبة 5,2% و 6,2% على التوالي. وكانت اعلى نسبة عزل للجراثيم من نماذج البراز التي جمعت خلال شهر نيسان. وقد اظهرت طريقة حضن ماكنتوش عزل نقي للجراثيم اللاهوائية لمطثيات بيرفرنجس، بينما امكن الكشف بطريقة ناقوس الشمعة عن 56 عزلة من اصابات مشتركة لجراثيم هوائية واخرى لاهوائية.


Article
A Study on Humoral Immunity and Oral Bacterial Diversity in Patients with Trichomonas Tenax Infection

Authors: Batool M. Mahdi --- Waffa Hazim --- Bassma Maki --- Ali Ghalib M. Mahdi --- et al.
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2009 Volume: 8 Issue: 4 Pages: 339-343
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGROUND:Trichomonas tenax (T tenax) is a protozoan that inhabit the oral cavity of poor oral hygiene. It is found in the dental caries, tartar and periodontal area. It feed on the normal flora of the mouth. It had been found to cause pulmonary infection and respiratory diseases in immunocompromised patients and patients wi advanced cancer.thOBJECTIVE:To determine the humoral immune response to Trichomonas tenax and studying if there is any relation with specific bacteria.METHODS:Forty patients who consult Al-Kindy Teaching hospital –maxillofacial and dental department from June-2008 to January -2009.Two gingival swabs were taken from those patients, one examined directly for the presence of T tenax by light microscope and other swab was cultured on Blood, Chocolate and MacConkey's agar for isolation of oral bacteria. Blood was collected from patients for estimation of serum IgG, IgA, IgM, C3 and C4 levels by using radial immune diffusion method.RESULTS:The study group consists of forty individuals, their ages range from 6-65 years. Male more than female (22:18), thirty of them was smoker, 25% of them were positive for T tenax. The types of bacteria that were isolated are a normal flora of the mouth like Streptococcus viridans. In spite of the level of Immunoglobulins and complement in both groups lie within normal values, there was a significant increased in serum IgM level and significant decreased in serum IgG, IgA, C3 and C4 level.CONCLUSION:There was increased in the prevalence of T tenax infection due to low social class and low oral hygiene .There was no specific bacteria that was confected with it. Lastly, increased in T tenax infection when there is an immune suppression as in advanced cancer patients and on radiotherapy and or chemotherapy.


Article
EVALUALATION OF ANTIMICROBIAL ACTIVITY OF FRESH RHIZOMES EXTRACTION FROM ZINGIBER OFFICINAL
تقدير الفعالية المضادة للميكروبات لمستخلص جذور الزنجبيل Zingiber officinal

Author: Hanaa Khlil Ibrahim هناء خليل إبراهيم
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: Print:18138497 E; 24108456 Year: 2010 Volume: 9 Issue: 2 Pages: 1-8
Publisher: Basrah University جامعة البصرة

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Abstract

Investigation of antibacterial activity of ethanolic , methanolic and ginger oil were carried out in this study on Gram positive and Gram negative pathogenic bacteria . The study exhibited these bacteria have variable susceptibities against these extracts and depend on type of bacteria . The notes was shown the highest effect and wide diameter of growth inhibition zone against Escherichia coli .

لقد تم دراسة الفعالية المضادة للميكروبات لمستخلصات الايثانول والميثانول والمستخلص الزيتي لجذور الزنجبيل. وقد اختبرت بعض انواع من البكتريا الموجبة والسالبة. حيث بينت الدراسة ان هذه البكتريا لها حساسية مختلفة اتجاه هذه المستخلصات وحسب نوع البكتريا وقد لوحظ ان التاثير الاكبر للمستخلصات كان على جرثومة . Escherichia coli

Keywords

ethanolic --- zingibeeaceae --- bacteria

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