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Article
Inhibitory Effect of Lactobacilli Filtrate on Klebsiella Pneumoniae Biofilm

Author: Harith J.F. Al-Mathkhury
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2012 Volume: 11 Issue: 2 Pages: 168-179
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGROUND:The formation of biofilms by K. pneumoniae is thought to protect the bacteria from antibiotics since these entities are highly resistant to such antimicrobial agents.OBJECTIVE:Investigating the role of Lactobacillus spp. supernatant in eliminating K. pneumoniae biofilms.METHODS:Three hundred and twelve specimens of urine, wounds and sputum were collected from patients attending five hospitals in Baghdad. All specimens were identified using biochemical tests and Api 20 E system. The ability of K. pneumoniae isolates to produce biofilm were evaluated using crystal violet staining technique in pre-sterilized 96-well polystyrene microtiter plates. The effect of MIC of Amikacin and Gentamicin was assessed on biofilm. The ability of acid and alkaline supernatants of Lactobacillus acidophilus and L. fermentum to inhibit planktonic as well as biofilm of K. pneumoniae K14, K15, K17 and K19 isolates were separately tested.RESULTS:Twenty two (39.29%) K. pneumoniae isolates were considered as high biofilm producers. Moreover, K. pnumoniae isolates; K14, K15, K17 and K19 produced the thickest biofilm. All biofilms treated with Amikacin and Gentamicin developed a presence of live cells when cultured on plate count agar. Neither neutralized supernatant of Lactobacillus acidophilus (Lb1) and L. fermentum (Lb2) nor sterile MRS broth has an effect on planktonic K. pneumoniae K14, K15, K17 and K19 isolates. Yet, results showed that lactobacilli acid supernatant developed a marked inhibitory effect. Significant differences (P<0.05) were found in O.D. and viable count between pre and post treatment of K. pneumoniae K14, K15, K17 and K19 biofilms with acid supernatants of Lb1 or Lb2. Unlike the Amikacin and Gentamicin treatment which left live bacterial cells, Lactobacillus supernatant left no live cells except for two cases; Lb1 against K17 and Lb2 against K15.CONCLUSION:Lactobacillus supernatant is a potent antimicrobial agent against K. pneumoniae biofilms


Article
Inhibitory Effect of Lactobacilli Filtrate on Klebsiella Pneumoniae Biofilm

Author: Harith J.F. Al-Mathkhury
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2012 Volume: 11 Issue: 2 Pages: 168-179
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGROUND:The formation of biofilms by K. pneumoniae is thought to protect the bacteria from antibiotics since these entities are highly resistant to such antimicrobial agents.OBJECTIVE:Investigating the role of Lactobacillus spp. supernatant in eliminating K. pneumoniae biofilms.METHODS:Three hundred and twelve specimens of urine, wounds and sputum were collected from patients attending five hospitals in Baghdad. All specimens were identified using biochemical tests and Api 20 E system. The ability of K. pneumoniae isolates to produce biofilm were evaluated using crystal violet staining technique in pre-sterilized 96-well polystyrene microtiter plates. The effect of MIC of Amikacin and Gentamicin was assessed on biofilm. The ability of acid and alkaline supernatants of Lactobacillus acidophilus and L. fermentum to inhibit planktonic as well as biofilm of K. pneumoniae K14, K15, K17 and K19 isolates were separately tested.RESULTS:Twenty two (39.29%) K. pneumoniae isolates were considered as high biofilm producers. Moreover, K. pnumoniae isolates; K14, K15, K17 and K19 produced the thickest biofilm. All biofilms treated with Amikacin and Gentamicin developed a presence of live cells when cultured on plate count agar. Neither neutralized supernatant of Lactobacillus acidophilus (Lb1) and L. fermentum (Lb2) nor sterile MRS broth has an effect on planktonic K. pneumoniae K14, K15, K17 and K19 isolates. Yet, results showed that lactobacilli acid supernatant developed a marked inhibitory effect. Significant differences (P<0.05) were found in O.D. and viable count between pre and post treatment of K. pneumoniae K14, K15, K17 and K19 biofilms with acid supernatants of Lb1 or Lb2. Unlike the Amikacin and Gentamicin treatment which left live bacterial cells, Lactobacillus supernatant left no live cells except for two cases; Lb1 against K17 and Lb2 against K15.CONCLUSION:Lactobacillus supernatant is a potent antimicrobial agent against K. pneumoniae biofilms


Article
Correlation between virulence factor and biofilm formation in Proteus spp.
العلاقة بين عوامل الضراوه وتكوين الغشاء الحياتي في جنس Proteus spp.

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Abstract

Urinary Tract Infection is an infection that caused by the members of the genus Proteus that depends mainly on the availability of virulence factors ;Various virulence factors including biofilm, swarming migration , polysaccharide ,heamolysin,protease, DNase, urease production weredetermined for 45Proteus isolates that obtained from clinical specimens of Urinry Tract Infection patient . The distribution of virulence factors was showed variation among the testedisolates and strain specific in most cases. All Proteus isolates showed 45 (100%)biofilm , polysaccharide andSwarming capabilities with different extents. High ureaseproduction was demonstrated in most isolates 40 (88.8%);In addition, they were abling to produce protease,DNase and heamolysin, in 30(66.6%) , 28 (62.2%) and26(57.7%) respectively. The result of susceptibility test for Proteus spp. against seven antibiotics commonly used to treat UTI infection caused by Proteus spp. ; P.mirabilis were 90% sensitive for Meropenem , 80% to Ciprofloxacin , 60% for both Cephalothin , Nitrofurantion , 50% , 40% and 30% for Amikicin, gentamicin and ceftriaxone respectively ,butP.vulgaris isolates were sensitive to 80% for Meropenem ,70% Ciprofloxacin and Nitrofurantion and sensitive to 60%,50% ,40 % and 30 % for Cephalothin, gentamicinAmikacinand ceftriaxone respectively.(This studyaimed to detect and evaluate the presence of some virulence factors in Proteus species isolates causing Urinry Tract Infection .

التهاب المسالك البولية هو التهاب يصيب جزء من المسالك البولية والناجمة عن الاصابه بجنسProteus spp.حيث يعتمد بشكل رئيسي على توافرعواملالفوعة . حددت عوامل امراضية مختلفة والمتضمنة الغشاء الحياتي والانتشار السطحيوانتاج السكريات المتعدد وقابليه انتاج انزيم اليوريز والدينيز والبروتيز والهييمولايسين ل 45 عزلة تم الحصول علية من مرضى التهاب المسالك البولية. لقد اظهرت النتائج تباينا في توزيع عوامل الفوعه بين العزلات المختبرة وهي خاصيه متعلقة بالسلاله في معظم الاحيان. اظهرت معظم العزلات(100%) 45 قابليات مختلفه على انتاج الغشاء الحياتي والسكريات المتعدده والانتشار السطحي, كانت العزلات قادره على انتاج انزيم اليوريزبنسبة عالية ( 88.8) 40 عزلة وبالاضافه الى قدرتها على انتاج انزيم البروتيزوالدنايز والهيمولايسبن بنسب 30(66.6%) ,28 (62.2%) و26(57.7%) . اظهرت نتائج اختبار الحساسيه لعزلات spp. Proteus ضد سبع مضادات حياتيه شائعه الاستعمال لمعالجه الاصابه بالتهاب المجاري البوليه الناتجه من spp. Proteus حيثاظهرت العزلات العائده لProteus mirabilis بانها حساسه للMeropenem وCiprofloxacinبنسبه 90%و80% والCephalothin وNitrofurantion بنسبه 60% وللAmikicin بنسبه 50%لل gentamicin و ceftriaxone بنسبه 40% و30% على التوالي اما بالنسبه لعزلات Proteus vulgaris فانها كانت حساسه بنسبه 80% Meropenem و70 % لكل من Ciprofloxacin وNitrofurantion وولل Amikacinو Cephalothin وgentamicin و ceftriaxone بنسبه 60%,50%,40% و30% على التوالي .(الهدف من الدراسة هوكشف وتقييم وجود عوامل الفوعة في عزلات محلية تابعة الى جنسProteus spp. تسبب الاصابه في المسالك البوليه ).

Keywords

Biofilm --- Swarming --- DNase.


Article
The Effect of some Variables on the Quantity of Exopolysaccharide Produced by Klebsiella pneumoniae
تأثير بعض المتغيرات على كمية متعدد السكريات الخارجي المنتج من Klebsiella pneumoniae

Author: Sumaya A. Al- Hamdoni سمية عدنان الحمدوني
Journal: Rafidain journal of science مجلة علوم الرافدين ISSN: 16089391 Year: 2018 Volume: 27 Issue: 3E Pages: 47-57
Publisher: Mosul University جامعة الموصل

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Abstract

The current study attempted to compare the production of exopolysaccharide (EPS) by a clinical isolate Klebsiella pneumoniae under different selected conditions using the colorimetric method, phenol-sulfuric acid. The results revealed that the chemically defined medium was the most efficient for the production of free bacterial exopolysaccharide. The type and concentration of carbon source were of critical role in determining the amount of the yield EPS. The elevated fructose concentration furnished the highest level while lactose promoted the lowest yield. The pH ranges between 6- 8 of the medium promote the maximum production; lower and higher than this range the production will decrease. In the batch culture vessel used in the current study, a bacterium inoculum of 3-5% (v/v) produced a valuable amount of EPS after three days of incubation

حاولت الدراسة الحالية مقارنة انتاج متعدد السكريات الخارجي من قبل العزلة السريرية Klebsiella pneumoniae تحت ظروف مختلفة باستخدام طريقة التقدير اللونية phenol-sulfuric acid. بينت النتائج ان الوسط الزرعي chemically defined medium كان الاكثر كفاءة لإنتاج متعدد السكريات الخارجي الحر. وان نوع وتركيز مصدر الكربون كان ذا دور أساسي في تحديد كمية منتج متعدد السكريات الخارجي. التراكيز المتزايدة من الفركتوز أعطت التركيز الأعلى بينما اللاكتوز اعطى التركيز الاقل من المنتج. مديات pH بين 6-8 للوسط الزرعي مهدت للإنتاج الأمثل، بينما عند المديات الاقل او الاعلى فان الانتاج قل. في وعاء الزرع المغلق المستخدم في الدراسة الحالية فان لقاح البكتريا 3-5 % (حجم / حجم) انتج كمية وفيرة من متعدد السكريات الخارجي بعد ثلاثة ايام من التحضين.


Article
Biofilm Shows Independency form Hemolysin Genes Arsenal in Methicillin Resistant Staphylococcus Aureus

Authors: Nihad Taha Mohammed Jaddoa --- Harith Jabbar Fahad Al-Mathkhury
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2018 Volume: 59 Issue: 4C Pages: 2184-2194
Publisher: Baghdad University جامعة بغداد

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Abstract

Normally, bacteria exposed to antibiotics at sub minimal inhibitory concentrations (MIC) inside the host. Therefore, the current study aimed to comprehend the association among hemolysins, biofilm, as well as gentamicin resistance in local MRSA isolates. Around 35 Staphylococcus aureus locally isolated from different clinical specimens were employed in this study. Methicillin resistance was detected via cefoxitin disk diffusion and mecA amplification methods. MIC of gentamicin was estimated by broth microdilution method. Hemolysin genes involving hla, hlb, hld, and hlg were determined using multiplex polymerase chain reaction (PCR) technique. Microtiter plate method was employed for biofilm assessment in the presence and absence of gentamicin at sub MIC. Moreover, atomic force microscopy technique was employed for confirming the effect of gentamicin on biofilm. The present findings revealed that methicillin resistant S. aureus (MRSA) constituted, nearly, 94.29% (33 isolates) of all S. aureus isolates. Around 12 (36.36%), four (12.12%), and 17 (51.51%) isolates were gentamicin-sensitive, intermediate, and resistant to gentamicin, respectively. hla and hld were located in 32 out 33 MRSA isolates. All MRSA isolates succeeded in forming biofilm; however, three (0.09%), 23 (69.69%), and seven (21.21%) isolates formed weak,moderate, andstrong biofilm, respectively. Gentamicin at sub MIC reduced the intensity of biofilm and the AFM confirmed this finding. In conclusion, very weak correlation linked the biofilm formation capacity and isolate MIC. On the other hand, possession of hemolysin genes seems has no correlation with biofilm formation. Nevertheless, gentamicin at sub MIC reduced the intensity of MRSA biofilm.

Keywords

MRSA --- hemolysin --- biofilm --- gentamicin


Article
DETECTION OF INTRACELLULAR ADHESION GENE (icaA and icaD)AND BIOFILM FORMATION STAPHYLOCOCCUS AUREUS ISOLATES FROM MASTITIS MILK OF SHEEP AND GOAT
الكشف عن جینات الالتصاق الداخل خلویة وتكوین الغشاء الحیوي في جرثومة المكورات العنقودیھ المعزولة من حالات التھاب الضرع في الأغنام والماعز

Author: Hasan I. Idbeis ; Mohammed H. Khudor حسن كریم ادبیس ،محمد حسن خضر
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: Print:18138497 E; 24108456 Year: 2019 Volume: 18 Issue: 2 Pages: 306-327
Publisher: Basrah University جامعة البصرة

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Abstract

In the present study, a total of 150 mastitis milk samples were collected from sheep andgoat (75 for each one) and were analyzed for the presence of S.aureus.. The obtainedresults indicate that this bacterium observed in 20% of these samples (21.33% from sheepand 18.66% from goat) .The study of antibiotic susceptibility test to 9 different antibioticsshowed that S. aureus was 100% resistant to penicillin and 100% sensitive tovancomycin, gentamycin , clarithromycin and chloramphenicol . whereas for cefoxitin(alternative to methicillin) resistance was 47%.There were a variable sensitivitypercentage for the rest of antibiotics: Tetracycline (70%), Ciprofloxacin (80%) ,Clindamycin (83%). The biofilm-forming ability of S. aureus was evaluated viamicrotiter plates and the result revealed that, all the studied isolates were either moderatebiofilm producer or weak biofilm producer while the non-biofilm producer and strongbiofilm producer were not detected among the tested isolate.The relationship betweenbiofilm formation and resistance to methicillin showed there as no significant differences(P>0.05) in the percentage of weak and moderate biofilm producers between MRSA andMSSA isolates. PCR analysis was applied to DNA extracted from S.aureus isolates frommilk samples .The results of PCR assay revealed that all S.aureus isolates gave positiveresults for both icaA and icaD genes (100%) with Product size 151 and 211 bp,respectively.Results of this study indicate that biofilm producing S.aureus have a major role player onthe occurrence of mastitis .In addition, there was high prevalence of MRSA isolates(47%) in mastitic milk at the study area


Article
Antibacterial and Antibiofilm Activity of Zinc Oxide Nanoparticles on Pseudomonas aeruginosa Isolates from Diabetic Foot Infections Grade 1
الفعالیة المضادة للبكتریا ولتكوین الغشاء الحیوي لدقائق أوكسید الزنك النانوي على بكتریا الزوائف الزنجاریة المعزولة من إصابات القدم السكري من الدرجة الثانیة

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Abstract:The aim of this study was to investigate antibacterial activities and disruption of biofilmstructure by zinc oxide nanoparticles (ZnO NP). Pseudomonas aeruginosa was chosen as anindicator of pathogenic because its natural resistance to antibiotics and its ability to formbiofilm on surfaces makes the cells impervious to therapeutic concentrations.Twenty one isolate were taken from patients suffering from diabetic foot ulcer grads 2infections who attended from AL-Kindy Teaching Hospital in Iraq. Different concentrationsof zinc oxide (NP) had been used (25-20000) μg/ml.The results showed that the high concentrations (500-20000) μg /ml were lethal tobacteria also the minimum inhibitory concentration (MIC) and sub (MIC) of zinc oxide npwas determined in this study. Some of the isolates were inhibit in concentration 100 μg/mland others inhibit in concentration 75 μg/ml and according to these result the sub–MIC were75 μg/ml to some isolates and 50 μg/ml to others, these concentration were inhibitor biofilmproduction.Our study indicates that zinc oxide nanoparticles could potentially be an antibacterialreagent to treat diseases caused by bacteria.

الخلاصة:Pseudomonas الھدف من ھذه الدراسة ھو للتحقیق من فعالیة اوكسید الزنك النانوي وثاثیره على بكتریابسبب قابلیتھا العالیة Pseudomonas aeruginosa وعلى قابلیتھا لانتاج الغشاء الحیوي. تم اختیار aeruginosaلمقاومة المضادات الحیویة وكذلك قدرتھا العالیة على انتاج الغشاء الحیوي.جمعت إحدى وعشرون عزلة من مرضى قدم السكري نوع الثاني من مستشفى الكندي التعلیمي في العراق، حیث20000 ) میكروغرام/مل. - استخدمت تركیزات مختلفة من أوكسید الزنك النانوي والتي كانت ( 2520000 ) میكروغرام/مل كانت قاتلة للبكتیریا وكان الحد الأدنى - أظھرت النتائج أن التراكیز العالیة ( 500من أوكسید الزنك النانوي لبعض العزلات 100 میكروغرام/مل و 75 میكروغرام/مل للاخرى. (MIC) للتراكیزالمثبطةكانت 75 میكروغرام/مل لبعض العزلات و 50 میكروغرام/مل للاخرى، حیث كانت ھذه التراكیز sub-MIC أما نتائجمثبطة لانتاج الغشاء الحیوي. تشیرھذه الدراسة إلى أن جزیئات أوكسید الزنك النانوي من المحتمل أن تستخدم كمضادحیوي وكذلك تستخدم لمعالجة الأمراضالتي تسببھا البكتیریا.


Article
Study of Virulence factor of Acinetobacter baumannii and detection of bap gene

Author: Hadi R. R. AL-Taati , Zahraa J. Jameel , Raghda A. Taha
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2018 Volume: 17 Issue: 1 Pages: 37-43
Publisher: Baghdad University جامعة بغداد

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Abstract

A Total of 150 clinical specimens from different clinical sources including burns, wounds, and urinary tract infections in Baquba General Teaching Hospital for the period from September 2016 to May 2017. 14 isolate were identified as A.baumannii. Sensitivity of 14 clinical isolates were tested against 14 antibiotics. and the result showed all isolate was MDR. All Acinetobacter isolates were investigated for the production of virulence factors which include the ability for the biofilms formation by microtiter plate method, and detection of efflux pumps. 92% of the isolates were biofilms former, and 85.7% of the isolates possess the efflux pumps. also investigate for bap gene responsible for biofilm formation.


Article
Capability of Lactobacillus acidophilus supernatant to inhibit production of lipase from methicillin-resistant Staphylococcus aureus
على تثبیط انتاج اللایبیز من العنقودیات الذهبیة المقاومة Lactobacillus acidophilus مقدرة طافي للمیثیسیلین.

Authors: Harith Jabbar Fahad حارث جبار فهد --- Hala Muayad Radeef حلا مؤید ردیف
Journal: Journal of university of Anbar for Pure science مجلة جامعة الانبار للعلوم الصرفة ISSN: ISSN:PISSN: 19918941/EISSN: 27066703 Year: 2011 Volume: 5 Issue: 2 Pages: 1-5
Publisher: University of Anbar جامعة الانبار

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The ability of acid supernatants of Lactobacillus acidophilus isolated from a vinegar weretested against methicillin resistant Staphylococcus aureus (MRSA) with lipolytic activity isolated from acnepimple. Results revealed a significant effect of cell free supernatant on MRSA isolates in comparison withcontrol. Furthermore, subinhibitory concentrations of acid supernatants found to be very effective ininhibiting the production of lipase from biofilm and planktonic cells of MRSA isolates. What's more, acidsupernatants of L. acidophilus showed inhibiting effect on the crude lipase in egg yolk agar plates

المعزولة من الخل ضد العنقودیات الذهبیة المقاومة Lactobacillus acidophilus الخلاصة:اختبرت قدرة الطافي الحامضي لبكتریامقارنة مع MRSA المحللة للدهون المعزولة من حب الشباب. اظهرت النتائج تاثی ا ر معنویا للطافي في عزلات (MRSA) للمیثیسیلنالسیطرة. كما وجد ان الت ا ركیز تحت المثبطة للطافي الحامضي كانت مؤثرة جدا في تثبیط انتاج اللایبیز من الخلایا الهائمة و الغشاء الحیاتيفضلا عن ذلك امتد التأثیر التثبیطي الى اللایبیز الخام في اطباق مح البیض. .MRSA لعزلات


Article
Study the effect of Lysostaphin, on methicillin resistant Staphylococcus aureus( MRSA) biofilm formation
دراسة تاثير اللايسوستافين على قابلية المكورات العنقودية الذهبية المقاومة للمثسلين على تكوين البايوفلم( MRSA)

Authors: Elaf B. Nori AL-Sheikh ايلاف باسم نوري الشيخ --- Hana S. Yosif هناء سليم يوسف
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2014 Volume: 55 Issue: 1 Pages: 93-100
Publisher: Baghdad University جامعة بغداد

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Abstract

This study included collection of 100 specimens from patients in AL-Kindy Teaching Hospital and teaching laboratories of Medical City Hospitals in Baghdad during the period from August to December 2012 ,these specimens differed in their sources which included 19 nasal swab, 16 wound swab,27 burn swab, 7 pus, 15 sputum, 10 corneal swab and 6 urine . Only 38 (38%) isolates was identified as Staphylococcus. In this study, 29 isolates (76.3%) were coagulase-positive (COPS), while only 9 isolates(23.6%) were coagulase negative (CONS), from total 38 isolates of Staphylococci. The distribution of Methicillin resistance among Staphylococcus spp. was investigated by disc diffusion method. In this study, 21 isolates (55.26%) showed resistant to the Methicillin while 17 isolates (44.73%) were sensitive. The ability to produce slime layer by MRSA isolates was also investigated and the results showed that all isolates of MRSA was produced slime layer when tested by tube method, but the amounts of adherent materials were differ among the isolates. However, the results by Congo red agar method showed that 57% of MRSA isolates produced strong slime layer and 43% of MRSA obtained negative result. Similarly the ability of MRSA to produce biofilm by tissue culture plate (TCP) was investigated and the results indicated that MRSA isolates showed highly and strong biofilm formation, and the OD value of biofilm formation ranged between 0.262 - 0.311. Additionally the OD value of biofilm formation significantly increased in addition of 1% glucose to the media. Statistical analysis showed slightly effect of lysostaphin under (5.625µg/ml MIC) on biofilm formation ability of (MRSA S3) optical density was reduced to (0.312 nm) in comparable with control group (0.389nm).

شملت هذه الدراسة جمع 100عينة سريريه من المرضى الراقدين في مستشفى الكندي التعليمي و المختبرات التعليمية لمدينة الطب في بغداد للفترة من حزيران ولغاية كانون الاول 2012.هذه العينات توزعت بين مسحات( للأنف (19), للجروح (16), الحروق (27) ,التقيحات (7), القشع (15 ), القرنية (10) ,والادرار(6). وتم تحديد 38 عزلة (38٪) تعود الى جنس .S.aureusوجد ان 29 عزلة (76.3٪) منها كانت موجبة لفحص اللأنزايم المخثر للبلازما Coagulase-Positive Staphylococci] (COPS)[ في حين اظهرت 9(23.6٪) نتيجة سالبة اتجاه هذا الفحص Coagulase-Negative] Staphylococci(CONS) .[ تم التحري عن مقاومة العزلات للمضادات الحيوية بواسطة طريقة الانتشار القرص على الاكار الصلب وقد اظهرت الدراسة ان (55.26٪) من العزلات كانت مقاومة للمثسلين في حين ان (44.73٪) من العزلات كانت حساسة تجاه هذا المضاد. وقد تم التحري عن قابلية العزلات MRSAعلى إنتاج الطبقة اللزجة وأظهرت النتائج أن جميع العزلات MRSAلها القابلية على انتاج الطبقة اللزجة عند اختباره بواسطة طريقةالانابيب باختلاف كمية المواد الملتصقة بين العزلات . ومع ذلك، أظهرت النتائج وفقا لطريقة اكار الاحمر الكونغو أن 57٪ من MRSA كانت لها قابلية قوية على تكوين الطبقة اللزجة و 43٪ من MRSA اعطت نتيجة سالبة . وبالمثل تم التحري عن قابلية MRSA على انتاج البايوفلم بواسطة الطبق الزرعي النسيجي (TCP) وأشارت النتائج إلى أن العزلات MRSA لها القابلية العالية والقوية على تكوين البايوفلم وقيمة OD من تشكيل بايوفلم تراوحت بين(0.262-0.311). بالإضافة إلى ذلك اظهرت زيادة كبيرة في قيمة OD لتكوين بايوفلم عند إضافة 1٪من الكلوكوزالى الوسط. أظهر التحليل الإحصائي تاثير قليل للايسوستافين تحت تركيز (5.625مايكروغرام/مل) على قابلية (MRSA -S3) على تكوين البايوفلم حيث انخفضت قراءة (OD)إلى (0.312 نانومتر) مقارنة بمجموعة السيطرة (0.389نانومتر ).

Keywords

lysostaphin --- S.aureus --- biofilm --- MRSA

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Year
From To Submit

2020 (2)

2019 (11)

2018 (21)

2017 (10)

2016 (7)

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