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Article
Comparative study of irradiation of blood by MS9 and Try pan blue methods on blood storage
دراسة مقارنة لتشعيع الدم بطريقتين لحفظ الدم باستعمال MS9 او باستعمال Tray Pan blue

Author: Fatehiya F. Hasan فتحيه فتحي حسن
Journal: IRAQI JOURNALOF COMMUNITY MEDICINE المجلة العراقية لطب المجتمع ISSN: 16845382 Year: 2011 Volume: 24 Issue: 1 Pages: 56-60
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Background: Transfusion-associated graft-versus-host disease (TA-GVHD) is a rare, but usually fatal, complication of transfusion. The risk associated with an individual transfusion depends on the number and viability of contaminating lymphocytes, the susceptibility of the patient's immune system to their engraftment and the degree of immunological disparity between donor and patient. The mainstay of prevention is gamma irradiation, which inactivates T lymphocytes whilst preserving the function of other blood cells. Objective: Comparison between the two methods of assay of MS9 and Try Pan blue assay to study the effect of radiation on blood storage Materials & Methods: Nine pints of blood had been taken from Notional Center for blood transfusion. From these pints, 255 samples were taken for test, 156 samples of blood exposed to high doses of gamma radiation of 15, 18, 20, 22, 24, 26, 28, and 30Gy. This radiation, obtained from 60Co available in Hospital of Radiotherapy and Nuclear Medicine in Baghdad, and the other 99 samples were used as a control. Blood Components are count by try pan blue in a way that nine pints of blood were utilized and from each pint several samples were used, eight of them were exposed to radiation of doses 15, 18, 20, 22, 24, 26, 28, and 30Gy, assay of try pan blue was used for counting the No. of red blood cells & lymphocyte cells whenever testing. The other pint used as a control sample before and after irradiation immediately.Results: From the results the assays by Try pan blue to count the viability of (lymphocyte cells and RBCs) was more accurate than assay by heamocounter MS9 (Haemocounter MS9 was used for automatic counts of blood cells) to know the period of the blood storage. Keywords: Irradiation, MS9, Try pan blue, Blood storage

Keywords

Irradiation --- MS9 --- Try pan blue --- Blood storage


Article
Effect of blood storage on certain hematological parameters
تأثير تخزين الدم في الدم بعض المعلمات

Authors: Bashar M. Saleh احمد يحى دلال، بشرى محمد --- Ahmed Y. Dallal Bashi
Journal: The Medical Journal of Tikrit مجلة تكريت الطبية ISSN: 16831813 Year: 2009 Volume: 1 Issue: 151 Pages: 171-180
Publisher: Tikrit University جامعة تكريت

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Abstract

When blood was stored outside the body some hematological and biochemical changes will takes place resulting in reduced red blood cells survival which is important drawback when transfused into the circulation of a recipient. This study was done to determine certain hematochemical effects on blood when stored during different periods of time (at 7 storage periods (from zero time up to 35 days) in both sexes using CPDA1 solution as preservative. Fifty blood donors (25 males and 25 females) who were attending the Central Blood Bank, Al-Zahrawi Hospital, Mosul (IRAQ) during the period from 1st October 2002 to 31st March 2003. A blood sample consisted of 50 ml was taken from each blood bag and this was divided into 7 portions, each contained about 7 ml of blood added into plain tubes. Blood in one of these tubes was analyzed immediately. The other six tubes were analyzed later on at intervals of 3 days, 1, 2, 3, 4, and 5 weeks. The blood samples were analyzed for hemoglobin (Hb), plasma (Hb), packed cell volume percentage (PCV %), methemoglobin and sulfhemoglobin. The results of this study showed that there was a significant decrease (P<0.05) in Hb, packed cell volume, methemoglobin and sulfhemoglobin while there was a significant increase (P<0.05) in plasma Hb. Moreover, when the Hematochemical parameters of donor blood samples of males is compared with that of female donors' blood sample at the same periods of storage no significant differences were noted between them. The possible effects of stored blood that it may undergoes unavoidable hematochemical changes that lead to decrease active desirable substances such as hemoglobin and viable red blood cells. So blood transfusion is preferable during less than 7 days of storage Moreover the Hematochemical parameters of donor blood samples of males was compared with that of female donors blood sample at the same periods of storage and no significant differences was noted between them

عندما تم تخزينها في الدم خارج الجسم بعض التغيرات الدموية والبيوكيميائية سوف يحدث مما أدى إلى انخفاض خلايا الدم الحمراء البقاء على قيد الحياة الذي هو العائق المهم عند نقله إلى الدورة الدموية للمستلم. أجريت هذه الدراسة لتحديد تأثيرات معينة على hematochemical الدم عند تخزينها خلال فترات زمنية مختلفة (في فترات تخزين 7 (صفر من الزمن تصل إلى 35 يوما) في كلا الجنسين باستخدام CPDA1 حل مثل المواد الحافظة. المتبرعين بالدم والخمسون (25 ذكرا و 25 أنثى ) الذين كانوا يحضرون بنك الدم المركزي، مستشفى الزهراوي، والموصل (العراق) خلال الفترة من 1 أكتوبر 2002 إلى 31 مارس 2003. عينة من الدم وتألفت من 50 مل واتخذ من كل كيس من الدم، وقسمت هذه إلى 7 أجزاء ، وأضاف كل الواردة حوالي 7 مل من الدم في أنابيب عادي. لقد تم تحليل الدم في واحدة من هذه الأنابيب على الفور. وقد تم تحليل الستة الأخرى الأنابيب في وقت لاحق على فترات من 3 أيام، 1، 2، 3، 4، و 5 أسابيع. و وقد تم تحليل عينات من الدم للهيموغلوبين (HB)، والبلازما (HB)، حجم الخلية معبأة نسبة (PCV٪)، وميتهيموغلوبين سلفهيموغلوبين. نتائج هذه الدراسة أظهرت أن هناك انخفاضا كبيرا (P <0.05) في الهيموغلوبين، خلية معبأة حجم، وميتهيموغلوبين سلفهيموغلوبين في حين كانت هناك زيادة معنوية (P <0.05) في الهيموغلوبين البلازما. وعلاوة على ذلك، عندما تتم مقارنة المعلمات Hematochemical من عينات الدم من الجهات المانحة من الذكور مع أن من عينة دم المتبرعين من الإناث في نفس الفترات من تخزين كبيرة لا ولوحظت الخلافات بينهما. الآثار المحتملة لتخزين دم أنه قد يخضع لتغيرات hematochemical لا مفر منه أن يؤدي إلى خفض المواد مرغوب فيه نشط مثل الهيموجلوبين وقابلة للحياة خلايا الدم الحمراء، لذلك نقل الدم هو الأفضل خلال أقل من 7 أيام من التخزين وعلاوة على ذلك hematochemical وبالمقارنة مع معلمات من عينات الدم من الجهات المانحة من الذكور مع الإناث من أن عينة دم المتبرعين في نفس الفترات من التخزين، ولوحظ عدم وجود فروق كبيرة بينهما


Article
8.EFFECTS OF DIFFERENT DOSES OF GAMMA RAYS AND ASCORBIC ACID CONCENTRATION ON HUMAN RBCS FOR CONSERVATION PURPOSE

Authors: Kharman A. Faraj كرمان اكرم فرج --- Samera H. Abdullah سميرة حسن عبدالله --- Sameen F. Muhammad سمين فاضل محمد
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2019 Volume: 17 Issue: 1 Pages: 50-56
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background:Blood preservation and the development of sterile collection sets made possible the developments in blood components preparation, storage and transfusion that we have in today's blood banks and transfusion services.Objective:To investigate the effects of gamma irradiation, ascorbic acid and the combined effect of both on the lifespan of erythrocytes through determining red blood cells hemolysis for conserving it as long as possible without any change in erythrocytes biophysical properties.Methods:The blood was drawn from 10 healthy (5 males and 5 females) volunteers. Sample has been irradiated using 137Cs source. Different concentrations of ascorbic acid were used as an anti-oxidative agent for erythrocytes in blood suspension samples. A spectrometer was used for recording the data.Results:The results showed that 25% of RBCs hemolysis occurred after irradiation with 5Gy of gamma ray during 5th week of storage time while in un-irradiated sample 33.8% of RBCs hemolysis occurred during the 5th week. 25% of RBCs hemolysis for 10 μM of ascorbic acid concentration started after 7th week while for control started after 4th week. The minimum rates of RBCs hemolysis observed in the samples which pre-treated with (7 and 10) μM concentrations of ascorbic acid then irradiated with 1 Gy.Conclusion:The results indicated that irradiation of human blood with a certain doses of gamma ray, treated with small concentration of ascorbic acid or both, the two factors together can protect the blood from hemolysis for a longer time and the minimum rate of red blood cells hemolysis was observed for 10 μM ascorbic acid concentration then irradiation to 1 Gy of gamma ray.Keywords;Gamma ray, ascorbic acid, blood storage, red blood cells, oxidative damageCitation:Faraj KA, Abdullah SH, Muhammad SF. Effects of different doses of gamma rays and ascorbic acid concentration on human RBCs for conservation purpose. Iraqi JMS. 2019; 17(1): 50-56. doi: 10.22578/IJMS.17.1.8

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