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Article
Is Chlamydia Pneumonia An Independent Risk Factor In Ischemic Heart Disease

Authors: Eman Sh. AL-Obeidy --- Eman Sh. AL-Obeidy
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2010 Volume: 9 Issue: 2 Pages: 151-154
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGROUND:Immune system may interplay between Chlamydia pneumoniae infection and ischemic heart disease (IHD).Major histocompatibility genes regulate innate and adaptive immunity..OBJECTIVE:This study was established to shed light on the possible association between ischemic heart disease (IHD) with Chlamydia pneumoniae infection and HLA antigens.PATIENTS AND METHODS:Microlymphocytotoxicity assay has been applied for HLA-typing of 150 blood samples of 100 IHD patients and 50 healthy normal controls, In addition enzyme linked immunosorbant assay (ELISA) used to detedct C pnuminiae IgA and IgG.RESULTS:An increased frequency of HLA-A*2, B*35 alleland HLA-A*2-B*35 haplotype was observed for patients group versus control group with P-value (0.0001, 0.05, and 0.001) respectively.CONCLUSION:This finding demonstrated that the HLA-B*35 positive haplotypes confer the C.pneumoniae-related risk for IHD. HLA-DR3,DR4 and DR7 might play a role in AIH susceptibility


Article
Molecular Diagnosis of Chlamydia pneumoniae in Coronary Heart disease Patients in Diyala City

Author: Hanaa N. Abdullah1 , Mohammed Abduldaim Salih2 , Esra F. Al-Azawi3
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2017 Volume: 16 Issue: 4 Pages: 79-88
Publisher: Baghdad University جامعة بغداد

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Abstract

Chlamydia pneumoniae has been highly associated with & implicated in the procoagulatory & inflammatory component of atherosclerosis. The present study has been designed to detect C. pneumoniae DNA and C. pneumoniae IgG and IgM antibodies among Iraqi patients with coronary heart disease and the association between the bacterium and risk factors. The study involved 71 patients diagnosed with (angina, myocardial infarction and atherosclerosis) and 30 healthy controls. Out from 71 patients, 28.2% were smoking, 26.8% were diabetic and 45.1% were hypertensive. Detection of anti- C. pneumoniae IgM and IgG antibodies was done by Enzyme-Linked Immunosorbent assay. C. pneumoniae IgG antibodies were detected in 57.7% of patients and 14.6% of healthy controls, while seropositivity of C.pneumoniae IgM antibodies were detected in 9.9% patients. DNA were extracted from blood samples and PCR was used for detection of the C. pneumoniae genes. Thirty DNA samples of the coronary heart disease patients were amplified by PCR using three genes 16SrRNA with 194bp and outer membrane protein (OMP) and were included as internal with 239bp and outer with 499bp both specific gene for C. pneumoniae. The results of all genes revealed that 10 patients (33.3%) were positive , while it was negative for all control group.

Keywords

C. pneumoniae --- CHD --- Molecular detection --- PCR.

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