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EXPRESSION OF CERTAIN ACTIVATION MARKERS, CD45RA, CD45RO AND CD11b ON THE SURFACE OF PERIPHERAL BLOOD LYMPHOCYTES ISOLATED FROM PATIENT WITH IDIOPATHIC PRETERM LABOUR
ظهور بعض من معلمات التنشيط على سطح الخلايا اللمفاوية للدم المحيطي والمعزولة من مريضات الولادات المبتسرة التلقائية مجهولة الاسباب.

Authors: Thoraya Hosaam Al-din ثريا حسام الدين --- Maha M. Al-Bayati مها محمد البياتي --- Nidhal Abdul Muhymen نضال عبد المهيمن
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2010 Volume: 8 Issue: 3 Pages: 20-24
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background: preterm labor (PL) is remaining the leading cause of non-anomalous prei natal mortalities.Objective: is to determine the association of PL on the expression of certain activation markers on the surface of peripheral blood lymphocytes (PBLs).Patients and methods: Thirty patients with idiopathic pre term labour (IPL) (group A) in addition to 30 healthy pregnant women of comparable gestational age groups (group B) were enrolled in this study. Blood samples were taken from both groups and lymphocytes were separated and stained with fluorescent labeled monoclonal antibodies against CD45RA, CD45RO and CD11b.Results: results indicated that there were a significant increase in the percentage of CD45RA in group A and reduction in the percentage of both CD45RO and CD11b in the same group.Conclusions: patients with IPL have a less tendency of the activity of lymphocytes.Key words: Idiopathic premature labour, activation markers, CD45RA, CD45RO, CD11b.

خلفية الدراسة: تضل الولادات المبتسرة المسبب الاساس للوفيات قبل الولادة. هدف الدراسة: تحديد مسؤولية الولادة المبتسرة على التعبير عن بعض معلمات التنشيط على سطح الخلايا اللمفاوية للدم المحيطي.المرضى وطرائق العمل: اخضعت للدراسة 30 مريضة تعاني من الولادة المبتسرة(المجموعة أ) بالاضافة إلى 30 إمراة حامل بنفس مدة الحمل (مجموعة ب ).أُخذَت عينات دم من كل النساء وفصلت الخلايا اللمفاوية بعدها صبغت هذه الخلايا بالاجسام المضادة وحيدة النسلية الخاصة بالمعلمات CD45RA,CD45RO إضافة إلى المعلم CD11b .النتائــج: أوضحت النتائج أن هناك زيادة معنوية بنسبة الخلايا التي تحمل المعلم CD45RAفي المجموعة(أ) وإختزال في نسبة التعبير عن المعلمين CD11b وCD45ROفي تلك المجموعة.الاستنتاجات: في الولادات المبتسرة هناك قلة في نشاط الخلايا اللمفاوية بدلالة التعبير عن بعض معلمات التنشيط على سطح الخلايا اللمفاوية. مفتاح الكلمات: الولادات المبتسرة , معلمات التنشيط , CD45RA,CD45RO, CD11b.


Article
NEUTROPHIL ACTIVATION THROUGH THE EXPRESSION OF CD11a, CD11b AND CD11c AND ITS ROLE WITH COMPLEMENT C3 AND C4 LEVELS IN PATIENTS WITH PRE-ECLAMPCIA

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Abstract

BackgroundThe leukocyte integrin that plays a major role in neutrophil activities is CD11b. In addition to mediating neutrophil adherence to endothelial cells, CD11b binds to the complement component iC3b and directs phagocytosis and intracellular lysis of microorganisms.ObjectiveTo determine whether neutrophil activation, through the increased surface expression of the cell surface markers CD11a, CD11b, CD11c have a correlation with the values of complement components C3 and C4 in pregnant women with pre-eclampsia.MethodsThis study was conducted at the AlKadhemiya Teaching Hospital. Patients were 60 pregnant women in labour subdivided into three groups:Group A: 20 pregnant women with severe pre-eclampsia.Group B: 20 pregnant women with mild-moderate pre-eclampsia.Group C: 20 normotensive pregnant women (control group).We performed the following laboratory measurements for all groups: total white blood cell WBC count, cell surface expression of CD11a, CD11b, CD11c by direct immunofluorescent technique, and serum complement C3 and C4 levels by radioimmunodiffusion method (RID).ResultsThere was a significant difference in neutrophil activation as detected by the cell surface expression of CD11a, CD11b, CD11c being higher in the pre-eclamptic group than the control group. The incidence of neutrophil activation was significantly higher among patients in group A compared to the other groups. There was a significant difference in the serum level of C3 and C4 in the pre-eclamptic group being higher in group A and B than group C.ConclusionsNo correlation was found between the markers of neutrophil activation (CD11a, CD11b, CD11c) and the serum levels of (C3, C4) in the pre-eclamptic group. The incidence of adverse maternal and neonatal outcome is significantly higher among patients in group A compared to the other two groups.Key wordsPreeclampsia, complement serum level, CD11a, CD11b, CD11C, neutrophil.

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