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Article
ESTIMATION CELL MEDIATED IMMUNITY IN SOME IRAQI PATIENTS WITH TYPE 1 AND 2 DIABETES MELLITUS
قياس المناعة الخلوية في بعض المرضى العراقيين المصابين بالنوع الأول والثاني من مرض السكري

Author: Mohammed A. Saleh محمد عبد الدايم صالح
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2012 Volume: 53 Issue: 2 Pages: 292-297
Publisher: Baghdad University جامعة بغداد

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Abstract

Cell-mediated immunity was estimated in diabetic patients by evaluation a level of CD4 and CD8 in type 1 and 2 diabetic patients. Of the 33 patients with type 1 diabetes mellitus 17 were males and 16 were females, as well as the type 2 diabetes mellitus patients were made up of 17 males and 16 females. Both groups attend the outpatient Clinic in the National Diabetes Center, Al-Mustansiriya University. Besides that, the results of this study recorded that the mean of age among patients with type 1 diabetes mellitus was 23.42 ± 4.25 y whereas mean of age among second group was 51.27± 7.83 y with highly significant difference was noticed between them. In addition, the result of this study indicated that duration of disease among type1diabetic patients was 7.15± 2.55 y while 11.45± 6.49 y among second group with highly significant difference was noticed between them. For CD4 lymphocytes, there was a highly significant decrease (P<0.001) in the percentage means of the CD4 cells in type2 diabetic patients, as compared with type 1 diabetic patients. For CD8 lymphocytes, there was a decrease in the percentage of these lymphocyte in the peripheral blood of the type 2 diabetic patients, as compared with type 1 diabetic patients with highly significant importance was recorded between them. In conclusion, this study proved that impaired cell mediated immunity is prevalent in type 2diabetic patients, thus the change level of cytotoxic T-lymphocytes (CD8) and helper T- lymphocytes (CD4) might be responsible for depressed immune response in patients with type 2 diabetes

تم قياس المناعة الخلوية في مرضى السكري بواسطة تقييم مستوىCD4 وCD8 في مرضى السكري النوع الأول والثاني .مامجموعه 33 مريض مصابين بمرض السكري النوع الأول والمتضمنة 17 ذكر و16 أنثى وبالإضافة إلى مرضى السكري النوع الثاني والمتكونة من 17 ذكر و16 أنثى.مجموعتي المرضى كانوا من المراجعين للعيادة الطبية الخارجية في المركز الوطني السكري التابع للجامعة المستنصرية، إلى جانب ذلك سجلت نتائج هذه الدراسة أن معدل العمر بالنسبة لمرضى السكري النوع الأول كان 23.42± 4.35 سنة وبينما معدل العمر بالنسبة للمجموعة الثانية كان 51.27 ± 7.83 سنة مع وجود فرق معنوي عالي بين المجموعتين وبالإضافة إلى ذلك فان نتائج الدراسة أظهرت أن متوسط فترة الإصابة بالمرض لمرضى السكري النوع الأول كانت 7.15 ± 2.55 سنة بينما كانت 11.45 ± 6.49 سنة بالنسبة للمجموعة الثانية مع وجود فرق معنوي عالي بين المجموعتين. فيما يتعلق بالخلايا اللمفاوية نوعCD4 تم تسجيل انخفاض معنوي عالي (P<0.001) في معدل هذه الخلايا في مرضى السكري النوع الثاني بالمقارنة مع مرضى السكري النوع الأول وإما يتعلق بالخلايا اللمفاوية نوعCD8 تم تسجيل نقص في نسبة هذه الخلايا في دم في مرضى السكري النوع الثاني بالمقارنة مع مرضى السكري النوع الأول مع وجود فرق معنوي عالي مهم بين المجموعتين . كنتيجة نهائية فأن هذه الدراسة أثبتت بشكل واضح وجود خلل في المناعة الخلوية في مرضى السكري النوع الثاني، لذلك فأن تغيير مستوى الخلايا اللمفاوية السمية CD8 والخلايا اللمفاوية المساعدة CD4 ربما تكون مسئولة عن نقص الاستجابة المناعية في المرضى المصابين بمرض السكري النوع الثاني


Article
Immunological aspects of cystic echinococcosis in Erbil
الجوانب المناعية لداء المشوكات الكيسي في أربيل

Author: Hadi M. A. Al-Sakee هادي الساقي
Journal: Zanco Journal of Medical Sciences مجلة زانكو للعلوم الطبية ISSN: 19955588/19955596 Year: 2011 Volume: 15 Issue: 2 Pages: 1-9
Publisher: Hawler Medical Univeristy جامعة هولير الطبية

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Abstract

Background and objectives: Echinococcus granulosus exists as a complex of different strains that differ in a wide variety of criteria that impact on the epidemiology, immunology, pathology and control of hydatid disease. This study was undertaken to investigate both humoral and cellular immune responses that are developed against hydatid cysts in Erbil.Methods: Thirty patients (9 males and 21 female) with surgically confirmed cystic echinococcosis and 10 apparently healthy individuals were included in this study. IgG ELISA was performed to asses humoral immune responses. CD4/ CD8 ratio, eosinophil count and lymphocyte transformation response were done to asses the cellular immune responses. The level of IFN-γ and TNF-α was also assayed.Results: The sensitivity of ELISA to detect anti-hydatid antibodies was shown to be 83.33%. CD4 / CD8 ratio was significantly (P< 0.001) decreased in patients with cystic echinococcosis as compared to normal control group, while eosinophil count (P< 0.001), lymphocyte transformation response (P< 0.001) and IFN-γ level (P< 0.01) were significantly increased. In contrast the level of TNFα was non- significantly changed in echinococcosis patients.Conclusion: The current study showed that the local strain of Echinococcus granulosus induces both cellular and humoral immune responses, and the number of peripheral blood CD8 T cells was significantly increased in cystic echinococcosis patients. However, hyporesponsivness to hydatid specific antigens has not been induced.


Article
ALTERATION IN THE PHENOTYPE OF PERIPHERAL BLOOD T LYMPHOCYTE IN PATIENTS WITH IDIOPATHIC PRETERM LABOUR
التغيرات المظهرية على الخلايا اللمفاوية التائية للولادات المبتسرة التلقائية مجهولة الأسباب

Authors: Thoraya Hosaam Al-din ثريا حسام الدين --- Nidhal Abdul-Muhymen نضال عبد المهيمن --- Maha M. Al-bayati مها البياتي
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2008 Volume: 6 Issue: 1 Pages: 60-64
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Objective: The current study aimed to detect some changes occurred on the surface of T lymphocytes manifested by CD3, CD4 and CD8 molecules that may have a role in patients with idiopathic preterm labour. Setting: Al-Kadhimyia Teaching Hospital / Department of Obstetrics & Gynecology for a period of one year (March 2002-March 2003).Study design: Thirty patients with idiopathic preterm labour were enrolled in this study in addition to thirty healthy pregnant women as a control group. Blood samples were taken from both groups, lymphocytes were separated and immunofluorescent labeled by monoclonal antibodies to CD3, CD4 and CD8 surface markers. Results: Patients have a significant low percentage of these surface markers in comparison with control subject.Conclusion: The above findings confirm the suppression of cellular immunity in patients with idiopathic preterm labour.

خلفية الدراسة: وجد في الأبحاث الحديثة الخلايا اللمفاوية التي يعبر على سطحها مستضدات مختلفة لها دور أساسي في إحداث الولادات المبكرة. هدف الدراسة: هدفت الدراسة الحالية التحري عن بعض التغيرات على سطح الخلايا اللمفاوية متمثلة بمجاميع التمايزCD8,CD4,CD3 التي من الممكن أن يكون لها دور في إحداث الولادات المبتسرة مجهولة الأسباب .المواد و طريقة العمل: لتحقيق هذا الهدف فقد شملت الدراسة (30) سيدة في حالة ولادة مبتسرة تلقائية مجهولة الأسباب بالإضافة إلى (30) سيدة حامل سليمة لفترات حمل مقاربة لتلك الفترة البالغة للمجموعة الأولى ودون أية بينة لولادة مبكرة كمجموعة سيطرة. تم اخذ عينات دم من كل سيدة حيث فصلت الخلايا اللمفاوية من عينات الدم واجري لها حساب دقيق ثم حضرت سلايدات فلورسينية (وميضية ) خاصة لتجمعات التمايز الثالثة والرابعة والثامنة ثم اجري الفحص الوميضي المناعي المباشر لهذه الخلايا. النتـــائج: تمت مقارنة نسبة كل تجمع تمايزي لمفاوي بين المجموعتين فوجدنا نسبة اقل من كل من التجمع التمايزي الثالث والرابع والثامن في المجموعة الأولى مقارنة بمجموعة السيطرة وكان هذا الفرق ذا دلالة إحصائية كبيرة الأستنتاجات:أكدت النتائج الأثباط المناعي الخلوي لدى السيدات اللاتي لديهن ولادات مبتسرة.


Article
Functional and Developmental Analysis of CD4+CD25+Regulatory T Cells Under the Influence of Streptococcal MProtein in Rheumatic Heart Disease

Author: Zaman I. L. Al-Kaabi
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2012 Volume: 11 Issue: 1 Pages: 76-81
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRUCT:BACKGROUND:CD4+CD25+ regulatory T cells are known to suppress the immune response in general, these cellswere studied in the presence of streptococcal M protein which has an important role in thepathogenesis of rheumatic heart disease.OBJECTIVE:The purpose of this study was to determine the role of streptococcal M protein in naturallyoccurring CD4+CD25+ regulatory T cells (nTregs) function and development in rheumatic heartdisease Iraqi patients.METHODS:Streptococcus pyogenes was isolated for subsequent M protein extraction. Also, peripheral bloodnTregs and CD4+ T cells were isolated by using Magnetic Cell Separation System (MACS). Tissueculture system for isolated cells was performed in the presence and absence of M proteinstimulation. Cell count was performed, also, TNF-α, and IL-4 were determined in culturesupernatant using ELISA system.RESULTS:It was found a highly significant positive association between the number of the cellularproliferation for both nTregs and CD4+ T cells with or without streptococcal M protein stimulationin isolated cell culture systems (p < 0.01), but, there found a highly significant negative correlationbetween the mean number of nTregs and CD4+ T cells in mixed culture system in the absence ofM protein (r = -0.995). whereas, in the presence of M protein, there was a positive non-significantassociation between the mean number of both nTregs and CD4+ T cells (r = 0.353) (p > 0.05).Results obtained from ELISA test revealed that M protein-stimulated CD4+ T cells produced IL-4in very little amounts (< 4 pg/ml) in all cultures of samples and there was no significant differenceamong them. Whereas, TNF-α was produced in higher concentrations in the culture supernatantswhen compared with IL-4.CONCLUSION:Streptococcal M protein has an important role in increasing the proliferation of both CD4+CD25+regulatory T cells and CD4+ T cells, but the newer generation of CD4+CD25+ regulatory T cells inthe presence of M protein has lower suppressive activity against CD4+ T cells.


Article
CIRCULATING-PERIPHERAL BLOOD NATURALLY OCCURRING CD4+CD25+ REGULATORY T CELLS AND CD4+ T CELLS IN CHRONIC RHEUMATIC HEART DISEASE

Authors: Zaman I.L. Al-Kaabi زمن ابراهيم الكعبي --- Nidhal A.M. Mohammed نضال عبد المهيمن محمد
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2011 Volume: 9 Issue: 1 Pages: 24-33
Publisher: Al-Nahrain University جامعة النهرين

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Background: The development of autoimmune disease involves a breakdown in the mechanisms that control T cell tolerance to self antigens, these mechanisms are many and complex, and they integrate as immunoregulation. Among the cells that might be responsible for this regulation is a specific type of T cells which has the ability to downregulate the differentiation of helper cells or antigen specific effector cells. The main subset of these suppressor T cells is the naturally occurring CD4+CD25+ regulatory T cells (n Tregs) which are the most important and they derived as a functionally mature population from the thymus.Objective: The purpose of this study was to determine the correlation between the numbers of circulating CD4+CD25+ regulatory T cells (nTregs) and CD4+ T cells in chronic rheumatic heart disease patients.Methods: Peripheral blood samples were taken from 48 Iraqi patients with chronic rheumatic heart disease (CRHD). Lymphocytes were isolated from the peripheral blood, nTregs and CD4+ T cells; also, cell numbers were detected by using immunofluorescence technique.Results: In general, nTregs were found in lower numbers in the peripheral blood of CRHD patients in different study groups than in healthy control group, whereas, CD4+ T cells were found in higher numbers in some of patients than controls. Also, our results revealed that there was a significant negative correlation between naturally occurring CD4+CD25+ regulatory T cells and CD4+ T cells in all study groups.Conclusions: Our finding confirmed that there is a significant correlation between circulating nTregs and CD4+ T cells in chronic rheumatic heart disease.Key words: CD4+CD25+ regulatory T cells, CD4+ T cells, chronic rheumatic heart disease.


Article
Evaluation Of the Immunopatho Role Of Interleukins IL17, IL21,and CD4+,CD8+ T cells In Patients With Type 1 Diabetes In The City of Baquba
تقييم الدور المناعي لل CD4+ ,IL-21 ,IL-17, CD8+ في مرضى داء السكري النمط الاول في مدينة بعقوبة

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Background: Diabetes Mellitus (DM) is a heterogeneous group of high blood sugar hyperglycemia and produces insulin, insulin action , or both. Diabetes is caused by the inability of beta cells in the pancreas. The production of these insulin cells is little or no. This is known as type 1 mellitus diabetes (Insulin-dependent T1DM) Insulin-dependent diabetes mellitus.Objective:To evaluate the immune role of some of the immunological indicators of patients with type 1 diabetes by identifying the IL17 and IL21 concentrations, also The CD4 + and CD8 + T cells were identified by the Sandwich ELISA test.Patients and Methods: Blood samples were collected from 70 patients with type 1 diabetes (diabetes mellitus type 1) after diagnosis and 20 samples of healthy people were collected as a control group, an the study has been done during the period between October 2016 and May 2017.Results: The results of IL17 level showed a decrease of IL17 in patients with type 1 diabetes 114.31 ± 103.78 pg / ml compared to control group126.54±81.48pg / ml with no statistically significant difference (p> 0.05),and the results of IL21 level showed an crease of IL-21 in patients with type 1 diabetes , 209.40 ± 294.78 pg / ml compared with healthy 189.54 ± 274.82 pg / ml with no significant difference.The results showed a decrease in the concentration of CD4 + T cells in the group of patients 5.18±4.59 pg / ml compared to the healthy group whose concentration level was 5.52 ± 3.47 pg / ml.Also the results showed a high concentration of CD8+toxic T cells in the group of patients as it was 5.54 ± 7.39 pg / ml compared to the healthy group. The concentration of toxic T cells was 3.74 ± 3.57 pg / ml. There was no significant difference between the study groups for CD4 + and CD8 +.Conclusion: A number of pro-inflammatory cytokines (IL17) and inflammatory cytokines (IL21) as well as cellular immunity of CD4+ and CD8+have an important role in the development of type 1 diabetes in children and affect the immune response in the body through immunodeficiency.

خلفية الدراسة: أُجريت هذه الدراسة في وحدة السكري والتي كانت ضمن أستشارية مستشفى البتول للولادة والاطفال في مدينة بعقوبة خلال المدة من17/10/2016 م الى 17/5/2017 م.اهداف الدراسة: هدفت هذه الى تقييم الدور المناعي الاِمراضي لبعض المؤشرات المناعية للمرضى المصابين بداء السكري النمط الاول عن طريق تحديد تراكيز البين الأبيضاضي السابع عشر (17-IL) , والبين الابيضاضي الحادي والعشرين (( IL-21 , و أيضاﹰ تم قياس مستوى جزيئات (CD4+) وكذلك (CD8+) الذائبة والتي من خلالها تم الاستدلال على مستوى الخلايا التائية المساعدة T-helper cells والخلايا التائية السُمية T-cytoxic cellsحيث تم تحديدها كمياً بأستخدام اختبار الادمصاص للأضداد المناعية المرتبط بالأنزيم Sandwich ELISA test.المرضى والطرائق: تم جمع 70 عينة دم من مرضى السكري النمط الاول ( Diabetes mellitus type 1) بعد التشخيص من قبل الطبيب الأخصائي في وحدة السكري في استشارية مستشفى البتول للولادة والاطفال, بحسب المعايير المعتمدة من قبل منظمة الصحة العالمية لتشخيص مرضى السكري النوع الاول وتم أخذ 20 عينة دم من أشخاص أصحاء ظاهرياﹰ تم أعتمادها كمجموعة سيطرة. النتائج: تم تحديد مستوى تركيز الحركيات الخلوية التي تم قياسها في هذه الدراسة حيث اظهرت النتائج بالنسبة لمستوى تركيز البين الابيضاضي السابع عشر IL-17 انخفاضاً لدى المرضى المصابين بداء السكر من النوع الاول 103.78) ± 114.31 ) pg/ml مقارنة بمجموعة السيطرة ( 81.48 ±126.54 ) pg/ml مع عدم وجود فرق معنوي ذو دلالة احصائية.كما أوضحت النتائج أرتفاعاً في مستوى تركيز البين ابيضاضي الحادي والعشرون IL-21 لدى مجموعة المرضى (78294.± (209.40 pg/ml مقارنة بمجموعة الأصحاء ( 274.82 ±(189.54 pg/ml مع عدم وجود فرق معنوي. تم دراسة تأثير مستوى جزيئات الخلايا التائية المساعدة والسمية الذائبة والتي لها دور كبير في مرض السكري النوع الاول ( Diabetes mellitus type 1) حيث اوضحت النتائج انخفاضاً في مستوى تركيز جزيئات الخلايا التائية المساعدة الذائبة CD4+ وكان تركيزها اقل في مجموعة المرضى اذ بلغ 4.59) ±(5.18 pg/ml مقارنة بمجموعة الاصحاء التي كان مستوى تركيزها اعلى 3.47) ±5.52( pg/ml مع عدم وجود فرق معنوي ذو دلالة احصائية.الاستنتاجات: بالنسبة لمستوى تركيز جزيئات الخلايا التائية السمية الذائبة CD8+ اظهرت النتائج ارتفاع مستوى تركيزها في مجموعة المرضى اذ كان 7.39)± 5.54) pg/ml مقارنة بمجموعة الاصحاء اذ كان مستوى تركيز جزيئات الخلايا التائية السمية الذائبة (573. ± 743.) pg/ml ولا يوجد اي فرق معنوي بين مجموعتي الدراسة.


Article
Depleted Uranium Effects on Immunophenotyphing of Human Lymphocytes in Southern Iraq
تأثير اليورانيوم المنضب على التنميط المناعي للخلايا اللمفاوية للدم المحيطي للأشخاص القاطنين جنوب العراق

Author: Rafid Abdul-Wahid رافد عبد الواحد عبد الكريم
Journal: IRAQI JOURNALOF COMMUNITY MEDICINE المجلة العراقية لطب المجتمع ISSN: 16845382 Year: 2009 Volume: 22 Issue: 4 Pages: 249-253
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Background: Several studies demonstrated the toxicity of depleted uranium (DU) to the function of immune cells; the results indicated that higher concentrations of DU were able to alter macrophage functionality in vitro in a concentration-dependent manner, which led to significant decrease to the T-cell proliferation. The results demonstrated that a short-term, in addition that high-concentration DU exposure was able to perturb rapidly the interaction between macrophages and T cells, and immune function. Objective: to investigate the effects of DU radiation on the human immune system by using phenotyping of peripheral blood lymphocytes of volunteers exposed at least 15 years to depleted uranium radiation from the bombing after gulf war in their residential area. Patients & Methods: Five milliliter of peripheral blood were taken from the arm of the 50 volunteers aged between 25 and 65,(means years 35.25 ± 3.3 years) for at least 15 years exposed to Depleted Uranium (DU) weaponry in their residential area in a district in northern Al- Basra City after the gulf war. Fifty age matched males, apparently healthy, and unexposed from Baghdad city away from the Depleted Uranium contaminated area served as a control group. Phenotyping of peripheral blood lymphocytes had been done by direct immunofluorescent microscopy using anti –CD 3(for T-cells detection), anti-CD4 (for T- helper-cells), anti CD8 (for T- cytotoxic /suppressor cells), anti CD21 (for B-cells) and anti CD56 (for natural killer cells). Results: A statistically significant reduction of Peripheral Blood lymphocytes (PBL) percentage bearing CD3, CD4, CD8, CD19, CD56 (P<0.01) between the exposed volunteers and control group. The mean percentage of CD4+/CD8+ ratio in exposed volunteers group was significantly (P<0.01) lowered in comparison to control group . Conclusion: Through this study we demonstrated that the chronic exposure to depleted uranium radiation can damage the cellular immune system and reduced immune cell number which impaired immune functionKeywords: depleted uranium, CD4, CD8, CD21, CD56, Lymphocytes, phenotyping,

تم اجراء هذه الدراسة المناعية على 20عينة دم اختيارهم بشكل عشوائي متعرضين لليورانيوم المنضب المتولد من القصف بعد حرب الخليج لمدة لاتقل عن 15 عاما في مناطق سكناهم حيث تم اختيار النماذج من منطقة الرميلة شمال مدينة البصرة كذلك تم اختيار 10 اشخا ص غير متعرضين كمجموعة سيطرة من بغداد .تضمنت الدراسة تحديد المعلمات المناعية لخلايا الدم اللمفاوية المحيطية باستعمال اختبار الوميض المناعي المباشر ( imunofluorescence ) حيث تم في هذه الدراسة استخدام الأجسام المضادة للخلايا المفاوية التائية التي تحمل المعلم المناعي CD3,CD4,CD8,CD19,CD56 كذلك تم الكشف عن النسبة المئوية لCD4/CD8 حيث اظهرت النتائج وجود انخفاض معنوي واضح في الخلايا التي تحمل المعلم المناعي (CD3, CD4,CD8 CD19 ,CD 56) عند المقارنة مع النسبة المئوية لتلك الخلايا لدى مجموعة الغير متعرضين ( السيطرة) .كما اضهرت النتائج وجود انخفاضا معنويا واضحا في النسبة المئوية CD4/CD8 عند المقارنة بين مجموعة المتعرضين مع مجموعة السيطرة . .مما ادى الى الأستنتاج ان لليورانيوم المنضب تأثيرا سلبيا واضحا على الانواع المختلفة للخلايا المناعية للا شخاص المتعرضين لليورانيوم المنضب في تلك المنطقة بعد حرب الخليج مما ادى الى اضعاف الجهاز المناعي لدى مجموعة المتعرضين لليورانيوم المنضب

Keywords

depleted uranium --- CD4 --- CD8 --- CD21 --- CD56 --- Lymphocytes --- phenotyping


Article
Differential infiltration of CD4, CD8 and macrophage in oral squamous cell carcinoma (Immnoistochemical study)

Authors: Ahmed A. Ali احمد علي --- Riyadh O. Alkaisi رياض القيسي
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2012 Volume: 24 Issue: 3 Pages: 54-58
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Oral Squamous cell carcinoma (OSCC) is by far the most common malignant neoplasm of the oralcavity. It is an aggressive and lethal malignancy. The oral squamous cell carcinoma microenvironments containmany immune cells and their secretory products. Cell-mediated immunity and the innate immune system mayinteract with cancer cells and plays an important role in immune responses against cancer, CD8 cytotoxic Tlymphocytes (CTLs) are key effectors cells in antitumor immunity, while CD4 T cell have cardinal role in orchestratingantibody production and the activation of CD8 T cells and macrophages to exhibit antitumor functions. This suggeststhat immune system-related mechanisms have an effect on the development and spread of malignant diseases inhumansMaterials and methods: Thirty formalin-fixed, paraffin- embedded blocks of oral squamous cell carcinoma wereincluded in this study. H&E stain was done for each block for reassessment of histological examination. The expressionof CD4, CD8 and macrophages were detected by immunehistochemistry.Results: Immunehistochemical mean expression level of CD4, CD8 and macrophage in OSCC was (54.67) %, (51.08)% and (55.93) % respectively. Non significant correlation was obtained among the three studied infiltrates withclinicopathological findings of OSCC and with each other.Conclusion: Immunohistochemical expression of CD4, CD8 and macrophage infiltrates were observed in all studyingsamples of oral squamous cell carcinoma, however, statistically non significant correlation was found between themean expression level of these infiltrates with all clinicopathological findings of OSCC and with each other.Increasing expression level of CD4, CD8 and macrophage infiltration in all studied cases of OSCC suggest theirimportant role in oral carcinogenesis, however further studies with larger samples needed to identify their exactcorrelation with clinicopathological features of tumor


Article
The Impact of Tuberculosis on Immunomodulatory Markers: A Clinical Study

Author: Rafal Jaleel Al-Saigh
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2017 Volume: 14 Issue: 3 Pages: 567 -576
Publisher: Babylon University جامعة بابل

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Abstract

This study aims to investigate the levels of immunomodulatory markers like interluekin-6 (IL-6), tumor necrotizing factor-α (TNF-α), cell differentiation-4 (CD4) and CD8 levels in those patients with active tuberculosis (TB) disease in comparison with control group.41 Adults diagnosed with TB were included in comparison to 32 healthy individuals at Babylon health center for pulmonary diseases and TB. Descriptive data for patients and control group were collected by well-trained researcher following a structured questionnaire. In parallel, peripheral blood collected to determineIL-6, TNF-α, CD4 and CD8. Then the assessment for the association between clinical and descriptive data and immunomodulatory markers levels was investigated statistically.The majority of TB patients were males (56%) and 71% were resident in rural areas;47% of them were living in middle socioeconomic state, moreover, 47% of TB cases had diabetes, furthermore, 51% had chronic obstructive pulmonary diseases, 12% had hypertension and 39% of them had chronic anemia with 47% smokers with no significant difference versus control. Following to that, there was highly increased in IL-6 and TNF-α levels in TB patients versus control (P<0.001), with low CD4 level versus control (P<0.001). While there was no significant change shown in CD8 levels versus control and this might highly be correlated with 30% of abnormal liver function tests among TB patients. A high proportion of TB patients have low CD4 level mostly associated with active disease. Moreover, the increase of IL-6 and TNF-α levels suggests a inverse impact on CD4 level which closely associated with the outcome of the disease.

Keywords

Tuberculosis --- Immunomodulatory markers --- IL-6 --- TNF-α --- CD4 --- CD8.


Article
Study of HLA-class II Serotyping and cellular immunity CD4+T, CD8+T cells in Iraqi patients with Rheumatoid arthritis
دراسة التنميط المصلي لكريات الدم البيضاء و المناعة الخلوية للخلايا التائية المساعدة والسمية لدى المرضى العراقيين المصابين بالتهاب المفاصل الرثوي

Authors: Hanaa N. Abdulla هناء ناجي عبدالله --- Amina N. Al-Thuwan امنه نعمة الثويني --- Mohammed Ibrahim Nadir محمد ابراهين نادر
Journal: Al-Nisour Journal for Medical Sciences مجلة النسور للعلوم الطبية ISSN: 26640562 26640554 Year: 2019 Volume: 1 Issue: 1 Pages: 98-107
Publisher: Al-Nisour University College كلية النسور الجامعة

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Abstract

Rheumatoid arthritis is a complex polygenic disease whose environmental and genetic factors contribute to both of the predisposition and developing of disease. The current study was conducted to determine the frequency of human leukocyte antigen (HLA-DR, -DQ) and correlate to this variation with disease severity and detected on the role of cellular immune response in pathogenesis of rheumatoid arthritis. Fifty blood samples were collected from RA patients and 50 healthy control group with no history of inflammatory arthritis. The HLA class II (DR, DQ) estimated serologically by using micro lymphocytotoxicity test. The result indicated that a highly significant frequencies of HLA-DR4 and DR53 antigens were observed in RA as compared to healthy group (P<0.001). Our results revealed that there are significant higher frequencies of HLA-DQ3 antigen (P<0.001) in RA group as compared to control groups. Additionally, the percentage of CD8+ has been decreased in patients in comparison with healthy group, whereas the percentage of CD4+ T-cell has been slightly increased in patients in comparison with healthy control group with nonsignificant differences. The results of this study proved that nonsignificant correlation which was observed between cellular immune response (CD4 and CD8) among patients.

يعد التهاب المفاصل الرثوي مرض اضطراب مناعي ذاتي وتساهم العوامل البيئية والوراثية مساهمة شديدة في الاستعداد للإصابة بالمرض. اجريت الدراسة الحالية للتحري عن مستضدات الكرية البيضاء البشري النمط الثاني( HLA ,وعلاقتها في اختلاف شدة المرض والتحري عن دور الاستجابة المناعية الخلوية في Class-II- HLA-DR ,-DQ) عينة من الاشخاص 50 عينة دم من مرضى التهاب المفاصل الرثوي و 50 التسبب في التهاب المفاصل الرثوي. جمعت الاصحاء ظاهريا. تم قياس تنميط مستضدات الكريات البيضاء مصليا( ( باستخدام فحص سمية الخلايا HLA-DR,-DQ ). وأظهرت النتائج بأن هنالك زيادة معنوية في تكرار مستضدات الكريات البيضاء Lymphocytotoxicity اللمفاوية ( HLA DR4البشرية ( ( -) لدى مرضى التهاب المفاصل الرثوي مقارنة بالأصحاء DR53,HLA. فضلا عن P<0.001) وجود زيادة في تكرار مستضدات الكريات البيضاء البشرية (وبفارق عالي المعنوية في مرضى التهاب HLA-DQ3) المفاصل الرثوي مقارنة بالأصحاء( CD8+T . بالإضافة الى ذلك, انخفاض نسبة الخلايا التائية السمية ( P<0.001) ) لدى مرضى التهاب المفاصل الرثوي مقارنة مع مجموعة الأصحاء ، بينما اظهرت النتائج ارتفاعا في نسبة الخلايا cells CD4+Tcell التائية المساعدة ( ) لدى المرضى مقارنة بمجموعة الأصحاء. تؤكد نتائج الدراسة الحالية عدم وجود علاقة s معنوية بين الخلايا( .(CD4+T and CD8+T cells

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