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Functional and Developmental Analysis of CD4+CD25+Regulatory T Cells Under the Influence of Streptococcal MProtein in Rheumatic Heart Disease

Author: Zaman I. L. Al-Kaabi
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2012 Volume: 11 Issue: 1 Pages: 76-81
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRUCT:BACKGROUND:CD4+CD25+ regulatory T cells are known to suppress the immune response in general, these cellswere studied in the presence of streptococcal M protein which has an important role in thepathogenesis of rheumatic heart disease.OBJECTIVE:The purpose of this study was to determine the role of streptococcal M protein in naturallyoccurring CD4+CD25+ regulatory T cells (nTregs) function and development in rheumatic heartdisease Iraqi patients.METHODS:Streptococcus pyogenes was isolated for subsequent M protein extraction. Also, peripheral bloodnTregs and CD4+ T cells were isolated by using Magnetic Cell Separation System (MACS). Tissueculture system for isolated cells was performed in the presence and absence of M proteinstimulation. Cell count was performed, also, TNF-α, and IL-4 were determined in culturesupernatant using ELISA system.RESULTS:It was found a highly significant positive association between the number of the cellularproliferation for both nTregs and CD4+ T cells with or without streptococcal M protein stimulationin isolated cell culture systems (p < 0.01), but, there found a highly significant negative correlationbetween the mean number of nTregs and CD4+ T cells in mixed culture system in the absence ofM protein (r = -0.995). whereas, in the presence of M protein, there was a positive non-significantassociation between the mean number of both nTregs and CD4+ T cells (r = 0.353) (p > 0.05).Results obtained from ELISA test revealed that M protein-stimulated CD4+ T cells produced IL-4in very little amounts (< 4 pg/ml) in all cultures of samples and there was no significant differenceamong them. Whereas, TNF-α was produced in higher concentrations in the culture supernatantswhen compared with IL-4.CONCLUSION:Streptococcal M protein has an important role in increasing the proliferation of both CD4+CD25+regulatory T cells and CD4+ T cells, but the newer generation of CD4+CD25+ regulatory T cells inthe presence of M protein has lower suppressive activity against CD4+ T cells.


Article
CIRCULATING-PERIPHERAL BLOOD NATURALLY OCCURRING CD4+CD25+ REGULATORY T CELLS AND CD4+ T CELLS IN CHRONIC RHEUMATIC HEART DISEASE

Authors: Zaman I.L. Al-Kaabi زمن ابراهيم الكعبي --- Nidhal A.M. Mohammed نضال عبد المهيمن محمد
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2011 Volume: 9 Issue: 1 Pages: 24-33
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background: The development of autoimmune disease involves a breakdown in the mechanisms that control T cell tolerance to self antigens, these mechanisms are many and complex, and they integrate as immunoregulation. Among the cells that might be responsible for this regulation is a specific type of T cells which has the ability to downregulate the differentiation of helper cells or antigen specific effector cells. The main subset of these suppressor T cells is the naturally occurring CD4+CD25+ regulatory T cells (n Tregs) which are the most important and they derived as a functionally mature population from the thymus.Objective: The purpose of this study was to determine the correlation between the numbers of circulating CD4+CD25+ regulatory T cells (nTregs) and CD4+ T cells in chronic rheumatic heart disease patients.Methods: Peripheral blood samples were taken from 48 Iraqi patients with chronic rheumatic heart disease (CRHD). Lymphocytes were isolated from the peripheral blood, nTregs and CD4+ T cells; also, cell numbers were detected by using immunofluorescence technique.Results: In general, nTregs were found in lower numbers in the peripheral blood of CRHD patients in different study groups than in healthy control group, whereas, CD4+ T cells were found in higher numbers in some of patients than controls. Also, our results revealed that there was a significant negative correlation between naturally occurring CD4+CD25+ regulatory T cells and CD4+ T cells in all study groups.Conclusions: Our finding confirmed that there is a significant correlation between circulating nTregs and CD4+ T cells in chronic rheumatic heart disease.Key words: CD4+CD25+ regulatory T cells, CD4+ T cells, chronic rheumatic heart disease.

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