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Article
The role of Human Cytomegalovirus infection in Iraqi brain tumor patients

Author: Elham Mudallal Amer, Ali. H .M.Ali Alkhfaji, Zuhar N .Hamad
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2017 Volume: 16 Issue: 4 Pages: 15-25
Publisher: Baghdad University جامعة بغداد

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Abstract

A total of (40) astrocytoma patients tissues were enrolled in the present study which include (20) normal surrounding areas from similar tissues used as a control group. The fourty cases represented by formalin fixed paraffin embedded brain tumor tissues blocks and these blocks were collected from the archives of histopathology laboratories at Neurosurgery Teaching Hospital in Baghdad. During the period 2014 to 2015. In our study, the astrocytoma patients were classified into four groups according to their grades (I, II, III, IV). A retrospective study of (40) paraffin embedded samples which were previously diagnosed as brain tumors along with normal unaffected tissues or tissue surrounding the tumors as control were selected from different Histopathology laboratories. All the slides of the paraffin-embedded samples were re-examined and specific sections were selected to be prepared for the techniques (CISH). The Paraffin-embedded samples were sectioned to several sections with (3-4 mm) thickens on charged slides Using Chromogenic insitu hybridization procedure (CISH technique) was used to detect the HCMV on the embedded tissues by light microscope. All grade have positive results for HCMV nucleic acids but the higher percentage (100%) was present in high grades astrocytoma grades (IV).

Keywords

Cytomegalovirus --- Brain tumor --- CISH --- HCMV --- PCR.


Article
Assessment of Co-Infection of Human Cytomegalovirus DNA and Epstein Barr-Virus (ZEBRA-Genes) in Tissues of Ovarian Tumors

Author: Ruqaya M. J. Awadh1 , Shakir H. Mohammed Al-Alwany2
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2019 Volume: 18 Issue: 2 Pages: 95-105
Publisher: Baghdad University جامعة بغداد

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Abstract

Infection by Epstein Barr-Virus (EBV) begins with a short replication phase. The virus remains in a latent phase, only entering the lytic phase in response to a cascade of transcriptional signals. These signals are triggered by the ZEBRA protein (BZFL1) along with Rta (BRLF1). Human cytomegalovirus (HCMV) is responsible for a lifelong persistent infection which ranged from 50% to 90% in adult population, and is related to either socioeconomic status or geographic location. HCMV infection might lead to buildup tumor cells via the protection of certain tumor cells from apoptosis and modulating angiogenesis. The study was designed to determine ZEBRA-EBV gene and DNA-HCMV infections in tissues from ovarian tumors. An 150 ovarian tissues were examined for ZEBRA-EBV gene with DNA-HCMV. Those samples belonged to (45) patients diagnosed with ovarian cancer ; (45) from benign ovarian tumors and (20) patients with borderline ovarian tumors as well as (20) apparently normal ovarian tissues. The detection of ZEBRA-EBV gene and DNA-HCMV were done by chromogenic In situ hybridization (CISH). The positive results of ZEBRA - EBV -CISH detection in malignant ovarian tumors, where 64.4% (29 out of 45 tissues) showed positive signals. While ,in the benign ovarian tumors group were 37.8 % (17 out of 45 tissues) ,followed by borderline ovarian tumors & the apparently healthy ovarian control tissues were 30% (6 out of 20 cases) and 7.5% (3 out of 40 cases), respectively. The present study shows the positive results of HCMV-CISH detection in malignant ovarian tumors, where 55.6% (25 out of 45 cases) showed positive signals, while, 44.4% negative signals which represented 20 out of 45 cases in this group. While, in the benign ovarian tumor group was 35.6% (16 out of 45 cases). Negative signals which in benign group represented 29 out of 45 cases constituted 64.4% .Whereas ,the positive results in borderline ovarian tumor group were 40% (8 out of 20 tissues), followed by the apparently healthy ovarian control tissues was 12.5% (5 out of 40 tissues). We concluded from this study, ZEBRA -EBV genes as well as HCMV-DNA positive signals in malignant, borderline and benign tumors tissues ,they suggest an important role for these viruses in the development of ovarian tumors in Iraqi patients.

Keywords

Ovarian Tumors --- ZEBRA –EBV --- HCMV --- CISH.


Article
Role of Topoisomerase II Alpha Gene Status on Selection of Anthracycline Therapy in Triple Negative Breast Cancer

Author: Mohammed Fawzi Abdulmehdi Alqanbar
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2017 Volume: 14 Issue: 1 Pages: 189 -197
Publisher: Babylon University جامعة بابل

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Abstract

Triple negative breast carcinoma [TNC], is peculiar to have hormone receptors negative [ER and PR] and lose HER2 receptor reactivity by immunostaining. Generally, TNC tumors carry a bad prognosis. Little is to be perceived about TOP2A gene amplification in triple negative breast cancer. This information may be of importance for the expectation of the influence of therapy. Thus the objective of this study is to evaluate topoisomerase II alpha gene status in triple negative breast cancer. A cross sectional study was done, from July 2014 to February 2015 in Oncology hospital of Baghdad Medical Complex. Only 28 patients with triple negative breast cancer were included [ER-, PR-, her2/neu-] other molecular types of breast cancer were excluded. All 28 cases were successfully hybridized using dual-color chromogenic in situ hybridization [DC-CISH][Zyto- Vision] probe kit for determination of topoisomerase II alpha gene status Patients mean age was [47.7 ± 8.8 SD]. Tumor stage was intermediate [stage II] in [57%] while 36% of cases were in stage III. Tumor grade was 46% for grade II and 54% for grade III. Regarding Ki-67, 54% of cases express Ki-67 more than 14% in comparison to 46% of them the expression was less than 14%.Regarding topoisomerase II alpha gene amplification, gene amplification was noticed in single case only as [3.6%] and it was low amplification. So TNBC is always associated with insignificant alterations of topoisomerase 2 alpha gene and thus may predicts poor response to anthracycline therapy in this molecular group of breast cancer.


Article
The Role of PTEN Gene Deletion In Prostate Cancer In Relation To Proliferative Capability of Malignant Cells

Authors: Nabeel Tawfeeq Kammuna --- Kaswr Mosa Al-Taraihi --- Asaad Abdullhamza Al-janabi
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2016 Volume: 13 Issue: 3 Pages: 701 -711
Publisher: Babylon University جامعة بابل

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Abstract

PTEN gene is a tumor suppressor gene located in 10q23,3 that encode dual- specificity protein and lipid phospatase with tensinhomologe. The PTEN protein signals adjust cell splitting up and express cells to enter normal cell death way. defeat of PTEN leads to over –foundation of AKt, which in circle , is related with unrestrained cell production. In this cross section study, we examined 50 paraffin-embedded blocks belongs to 50 patients with proved prostate cancer, All slides subjected to IHC KI67 Ab ( which is a parameter of proliferative activity of malignant cells), and to 1 molecular study of PTEN gene in all tissues by using CISH technique. The association between PTEN gene status examined by CISH technique with KI67 score performed by IHC technique , is highly significant, in that we get 71.43% (25/35) of no deletion cases had KI67 score ≤10% of cells, while in cases with PTEN gene deletion , we had 73.33% (11/15) of cases carry KI67 score >10% of cells.

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