research centers


Search results: Found 33

Listing 1 - 10 of 33 << page
of 4
>>
Sort by

Article
Exfoliative Cytological Changes in Peritoneal Fluid fromPatients on Peritoneal Dialysis

Authors: Liqaa R.Mousa --- Mutaz A.Al-Qazzaz --- Yarub I.Kattab
Journal: Al-Kindy College Medical Journal مجلة كلية الطب الكندي ISSN: 18109543 Year: 2008 Volume: 4 Issue: 2 Pages: 1-4
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

Background: Ambulatory peritoneal dialysis introduced by Popvich et al (13) in 1978 , consists of a four to five hours lavage of peritoneal cavity with 2000 ml of glucose solution .It remains a useful method for treating patients with end stage renal failure till renal transplantation becomes possible.Objectives: The aim of the study is to evaluate the value of cytological changes of mesothelial cells in dialysate patients.Methods: Within one year period, 32 cytological peritoneal fluid samples were collected from patients with end stage renal failure regardless of the underlying causes, admitted to the dialyzing unit in Kadimya Teaching Hospital. Smears were prepared and fixed in 95 % ethyl alcohol and then stained with H & E stain to be interpreted by the same pathologist.Results: Thirty two samples of peritoneal fluid were obtained from patients in peritoneal dialysis with a mean age of 54.8 years and male to female ratio of about 1.9: 1.Twenty two had short term dialysis were compared with 10 patients with long term dialysis. Gross examination of the samples revealed clear yellow fluid. Macroscopical examination showed no evidence of inflammatory cells with increased exfoliation, cellularity and three dimensional mesothelial cellular clustering pattern with increased nuclear size. No statistical significances were found in the changes seen in cytological smears between both groups but remarkable nuclear changes were shown in both of them. Conclusion: This study demonstrated that peritoneal dialysis of any duration can induce significant atypical changes in mesothelial cells. The pathologist needs to be aware of these changes and to include peritoneal dialysis in the list of other benign conditions that cause reactive mesothelial atypia.


Article
Urine Cytology in Patients withLong Standing Dialysis

Authors: Liqaa R Mousa --- Mutaz A.Al-Qazzaz --- Yarub I Kattab
Journal: Al-Kindy College Medical Journal مجلة كلية الطب الكندي ISSN: 18109543 Year: 2007 Volume: 4 Issue: 1 Pages: 1-4
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

Background: Dialysis is in common use to treat patients with end stage renal failure .However longstanding dialysis harboring some cellular changes in various body fluids. This study was conducted in order to detect these changes in urine.
Objective: The study was conducted to detect cellular changes in urine of patients with longstanding dialysis.
Method: Fifty-three urine samples were examined cytologically obtained from patients with longstanding dialysis during 6 months period. Freshly voided midstream urine samples were taken . Samples were centrifuged and 2 to 3 drops of sediments were smeared on 2 glass slides and fixed in 95% ethyl alcohol then stained with Hand E stain to be evaluated.
Results: Gross physical examination of all urine samples were neither purulent nor hemorrhagic .Microscopically there was increased exfoliation of urothelial cells except in 9 cases. No significant cytological atypia were seen in all urine samples. Excessive exfoliation in the absence of significant inflammation, hemorrhage and cytological atypia compared with control group.
Conclusion: The study revealed that some cytological changes do occur in the urothelial cells of patients with longstanding dialysis. These changes need further attention and study to disclose their causes.
Key words: Urine cytology, dialysis.

Keywords

Urine cytology --- dialysis


Article
5- EVALUATION OF ENDOSCOPY BASED METHODS (HISTOPATHOLOGY, CYTOLOGY AND UREASE TEST) FOR THE DETECTION OF HELICOBACTER PYLORI

Authors: Sawsan S Al-Haroon --- Sarkis K Strak --- Rafid Adil Abood
Journal: Basrah Journal of Surgery مجلة البصرة الجراحية ISSN: 16833589 / ONLINE 2409501X Year: 2009 Volume: 15 Issue: 1 Pages: 21-24
Publisher: Basrah University جامعة البصرة

Loading...
Loading...
Abstract

This study aimed to assess the accuracy of histopathology, brush cytology, andurease test in the diagnosis of Helicobacter pylori and to evaluate the effect of testduration on the sensitivity and specificity of positive urease test for the detection of H.pylori.Fifty patients [25 patients with gastritis group A and 25 patients with duodenal ulcergroup B] selected from those attending endoscopy unit for dyspeptic symptoms, wereenrolled in the study. Four endoscopic biopsies were taken from each patient. Onebiopsy from each of antrum and body were obtained for urease test (Urease test wasread at 30 min, 1, 4 and 24 hour after biopsy insertion into the reagent), and onebiopsy from each of antrum and body were used for histopathological examination.Antral brush cytology was taken also from each patient. The patients were consideredH. pylori positive when minimum concordances of 2 out of 3 tests (Histopathology,brush cytology, and urease test) were positive.Fourteen patients were positive for H. pylori in group A, in comparison to seventeenpatients in group B. The sensitivities of the histopathological examination, brushcytology, and urease test at 24 hours in group (A) were 58%, 79%, and 93%respectively. Corresponding figures for the specificity were 100%, 91%, and 46%respectively. While in group (B) the sensitivities were 82%, 82%, and 100% and thespecificities were 100%, 100%, and 88% respectively.It is concluded that among the invasive methods, the association of the urease testwith brush cytology constituted the best choice for confirming the diagnosis of H.pylori, due to the high sensitivity of the urease test and high specificity of brushcytology.


Article
An appraisal of urine cytology in the diagnosis of transitional cell carcinoma of the urinary bladder

Author: Tariq Mohamed Zeki * Msc (path) د. طارق محمد زكي
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2007 Volume: 49 Issue: 2 Pages: 261-264
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

Summary:Background: Urine cytology has an acceptable sensitivity, & specificity that justifies its use in the current diagnostic protocol.In Iraq transitional cell carcinoma (TCC) constitute about 62.1 % of bladder cancer (which ranks the third according to the results of Iraqi Cancer Registry 1995-1997). Urine cytology used in the primarydiagnosis, follow up, and in the screening programs of asymptomatic but high-risk patients.Patients and methods: This study was conducted on 93 patients, diagnosed or clinically suspected to have TCC of the urinary bladder attending to the Urological department in AL- Kadhimiya Teaching Hospital, AL-Yarmouk Teaching Hospital, and Baghdad Medical City. During the period from July 2004to July 2005. 76 were males & 17 were females.Urine cytological examination was done to those patients using two types of stain (hematoxylin and eosin, and papanicoloau stain) to detect malignant cells with the identification of the degree of differentiation, if possible, the cytological findings were correlated with the histopathological findings. And the cytological findings compared regarding each type of stain.Results:. Mean age of the patients was 59 year. Male:female ratio was 4.4:1. Main clinical presentation was hematuria (45.8%) of the total.Urine cytological examination has a sensitivity of 87.7%, specificity of 100% with an overall accuracy of 78% in the detection of TCC of urinary bladder. The main chief complaint of patients with TCC was haematuriawitch constitute 45.8% of the total. The percentage of high¬grade cases in urine cytology was higher than that in histopathology.the percentage of cases of TCC diagnosed by papanicoloau stain was more than that diagnosed by H & E (73.24% by pap stain versus 70.42% by H&E).Conclusion: Urine cytology has an acceptable sensitivity, specificity and an overall accuracy in the diagnosis of TCC of urinary bladder, and it offers a good chance for determination of the grade of the tumor especially in high grade tumor. Pap stain was better in the diagnosis of TCC.Keywords: Urine cytology, bladder cancer,


Article
THE ROLE OF ESTROGEN AND PROGESTERONE ON VAGINAL CYTOLOGY DURING PROLIFERATIVE AND SECRETARY PHASES OF MENSTRUAL CYCLE IN WOMEN WITH POLYCYSTIC OVARIAN SYNDROME

Authors: Huda R. Kareem هدى رشيد كريم --- Haider A. Jaafer حيدر عبدالرسول جعفر --- Zainab H. Hashim زينب حسن هاشم
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2017 Volume: 15 Issue: 1 Pages: 78-87
Publisher: Al-Nahrain University جامعة النهرين

Loading...
Loading...
Abstract

Background:In the course of cyclic hormonal fluctuation, cytological changes in the vaginal epithelium can be correlated with the phases of the menstrual cycle, this can be utilized in diagnosis of hormonal status in women with pathological cycles as in polycystic ovarian syndrome (PCOS). Objective:To evaluate the effects of estrogen (E2) and progesterone hormones in PCOS on the vaginal cytology in different phases of menstrual cycle, and its relation to ovulation period.Methods:A non-random sample of one hundred women were enrolled in this study, divided into two groups; 50 women free from PCOS, and 50 were PCOS according to the criteria of Rotterdam 2003. All were underwent hormonal assay including estrogen and progesterone on 2nd and 21st day of the cycle respectively, vaginal smears cytology on the 2nd day, 14th and 21st day of the cycle, stained with H&E, the (parabasal, squamous, leukocytes) were identified and counted, ultrasound examination was done on 14th day of the cycle, that divide the sample into ovulatory and an ovulatory subgroups accordingly.Results:At the 2nd day of the cycle, there was a significant reduction in squamous cell count, highly significant increase in leukocyte, E2 hormone level increased markedly, with a significant negative correlation to leukocytes cells in patients' groups at P≤0.05. Squamous cells of vaginal smear attained more acidophilic cytoplasm and increase in nuclear pyknosis. At the 14th day of the cycle, a significant increase in squamous cells count at P≤0.05, cornified squamous cells predominantly seen associated with low ovulation rates of 44%. Ovulation showed significant reduction in parabasal cells count in patients group at P≤0.05. At 21st day of the cycle, progesterone level was reduced with a significant reduction in squamous cells, parabasal cells and leukocytes counts in patients, with no significant correlation between progesterone and vaginal cytology cells, squamous cells consisted of mature cornified cells that seen predominantly in vaginal smear.Conclusion:Hormonal changes in PCOS presented mainly by the effect of elevated E2 hormone, that associated with increase in squamous cells count and cornification, with reduction in leukocytes and parabasal cells toward mid cycle. Progesterone hormone exert no significant effect on vaginal cytology in PCOS.Keywords: PCOS, vaginal cytology, ovulation

Keywords

PCOS --- vaginal cytology --- ovulation


Article
An evaluation of methods of inducing sputumproduction in patient with suspected lung cancer

Author: Maha Shakir Hassan
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2006 Volume: 48 Issue: 4 Pages: 383-386
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

Background : the major focus of respiratory cytology is the diagnosis of lung cancer , carcinoma of the lung is now reported to be the most commonly diagnosed non- Cutaneous malignancy in the world. Iraq has faced the increase in incidence of this lethal type of cancer. Sputum cytology is a convenient method of screening and diagnosing primary epithelial tumor of the lung which is of many types include fresh smear ,Sacccomanno smear, and mailing container method.
Methods : Sputum cytological study was done on 50 patients suspected to have pulmonary carcinoma prepared by fresh smear method ,Saccomanno method ,and mailing container method.One, two,or three samples taken from each patient.Slides were prepared and stained by H and E stain and examined thoroughly .The accuracy specificity, and sensitivity was found for each method for comparison.
Result and Conclusion : A careful and sometimes frequent sputum samples is very essential in the diagnosis of lung cancer .Preservation and fixation methods are found to facilitate more accurate diagnosis especially in areas far from hospital.


Article
Diagnostic Approach of Atypical Cells in Effusion Cytology Using Computerized Image Analysis

Author: Zainab A J Al-Obaidi
Journal: Al-Kindy College Medical Journal مجلة كلية الطب الكندي ISSN: 18109543 Year: 2007 Volume: 4 Issue: 1 Pages: 60-62
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

Background: Cytology is one of the important diagnostic tests done on effusion fluid. It can detect malignant cells in up to 60% of malignant cases.
The most important benign cell present in these effusions is the mesothelial cell.
Mesothelial atypia can be striking andmay simulate metastatic carcinoma. Many clinical conditions may produce such a reactive atypical cells as in anemia,SLE, liver cirrhosis and many other conditions.
Recently many studies showed the value of computerized image analysis in differentiating atypical cells from malignant adenocarcinoma cells in effusion smears. Other studies support the reliability of the quantitative analysisand morphometric features and proved that they are objective prognostic indices.
Methods: Sixty three cases of pleural and peritoneal smears, previously reported as benign (19) cases, malignant (21) cases or atypical (23) cases, were retrieved from the files. In each of these smears; nuclear area, perimeter, and roundness coefficient of 80-100 cell were determined at x400 magnification by the use of image analysis system.
Statistical analysis was performed using analysis of variance and Tukey's HSD test.
Results: The mean values of nuclear roundness, nuclear perimeter and nuclear area vary between the three groups (benign, atypical and malignant cells) by using analysis of variance (p > 0.01).
The value of nuclear roundness, perimeter and area did not differ significantly between benign and atypical cells (Tukey’s test: p<0.01).
On the other hand, the value of nuclear roundness, perimeter and area showed a significant difference between malignant and atypical cells(Tukey's test: p> 0.01).
Conclusion: In conclusion, our data suggest that cytomorphometry performed on effusion smear cells may provide important information for the differentiation of atypical cells from malignant cells, in which the values of atypical cells are closer to those of benign cells during the examination of pleural and peritoneal smears by the use of image analysis system.
Key words: Effusion cytology, Atypical cells, Cytomorphometry


Article
Cytological and bacterial analysis of transtracheal aspirate from stray cats in AL-Qadisiyah province/ Iraq

Author: Muthanna Hadi Hussain
Journal: Al-Qadisiyah Journal of Veterinary Medicine Sciences مجلة القادسية لعلوم الطب البيطري ISSN: 18185746 23134429 Year: 2017 Volume: 16 Issue: 1 Pages: 79-81
Publisher: Al-Qadisiyah University جامعة القادسية

Loading...
Loading...
Abstract

This study was conducted to count the cytological parameters; total protein (TP), white blood cells count WBCs and differential (WBCs); from transtracheal aspirate (TTA) and microbial detection from thirty cats in Al-Qadisiyah province. Both genders were involved and the cats ranged in 1-3 years old. The mean total protein measured by the spectrophotometer was (89±0.04) mg/dl, mean WBCs was (925±0.7) cell/µl and the mean differential WBCs record 61% alveolar macrophages, 31% eosinophils and 8% neutrophils. Microbial investigation has revealed that respiratory tract of cats have no flora and we could not observe any respiratory infection in the captured cats; it may be related to the physiological and immunological adaptation of the respiratory system through the presence of the alveolar macrophage with a high percentage concerning other WBCs. There was no obvious regard to gender or age on these limitations.

Keywords

cytology --- transtracheal --- total protein --- cat


Article
PITFALLS OF THYROID CYTOLOGY IN DUHOK-IRAQ
الاخطاء الخلوية للغدة الدرقية في دهوك - العراق

Loading...
Loading...
Abstract

Background: The current study was undertaken to determine the validity of thyroid fine needle aspiration cytology (FNAC) in Duhok-Iraq to clarify its traded diagnostic errors locally and broadly.Patients and Methods: All thyroid cytologic and histologic cases referred to Duhok Pathology Centers, between January 2013 and December 2016, were enrolled in this study. Cytologic findings were compared with their corresponding final histologic results. The validity parameters of cytology were assessed and cases showing cytologic and histologic non-conformance were re-evaluated to highlight the dependant cytologic pitfalls used locally and in the literature.Results: Of 553 thyroid biopsies with 81.6% benign and 18.4% malignant, only 125 cases had preoperative diagnostic cytology and subsequent histologic final diagnoses. Of these, apart from 2 unsatisfactory cases, only 6 (4.9%) cytologic reports were proved not to be matched with their corresponding histologic results. The remaining 117 (95.1%) cases showed complete agreement between the two evaluation tests. Malignancy was predicted by cytology in 82.9%with a sensitivity of 94.3% and specificity of 95.5%. All the 6 unmatched cases were aspirated blindly with no image guide, 4 were false positive resulting in 3.2%false positive rate and 89.2% positive predictive value. The remaining unmatched 2 cases were false negative cytologies that gave 1.6% false negative rate and 97.7% negative predictive value. Cytologically, 5 (83.3%) unmatched smears, 4 false positive and 1 false negative, appeared in smears of lymphocyte-rich thyroid lesions, particularly Hashimoto’s. The false positive pitfalls comprised 2 over diagnosis of hypercellular smears showing some features of papillary carcinoma, 1 overestimation of the large cells with nuclear atypia as follicular carcinoma and 1 over diagnosis of lymphoid hyperplasia as lymphoma. On the other hand, low cellular smears with unclear atypical lymphoid cells underdiagnosed low grade MALT lymphoma and unobvious cytologic criteria missed the diagnosis of papillary carcinoma.Conclusions: Lymphocyte-rich thyroid smears should be interpreted by experienced cytopathologists in the context of clinical, radiological and cytologic findings as suchcases may give certain cytomorphologic pitfalls that may decrease the cytologic validity. In suspicious cases, further tests should be justified to overcome the limitations and pitfalls of features when applied alone.

الخلفية والأهداف: أجريت الدراسة الحالية لبيان مدى ملائمة خزعة بالإبرة الدقيقة للخلايا الغدة الدرقية (FNAC) في دهوك العراق لتوضيح الأخطاء التشخيصية المتداولة محليا وعلى النطاق العالمي.المواضيع و طرق البحث: في هذه الدراسة تم تسجيل جميع حالات الغدة الدرقية التي فُحِصَتْ نسيجيا وخلويا بين فترة كانون الثاني 2013 الى كانون الاول 2016 في مركز دهوك للأمراض. وتمت مقارنة النتائج الخلوية مع النتائج النسيجية المقابلة لها. وتم تقيم مدى كفائة المعلمات الخلوية، الحالات اظهرت عدم توافق الخلوي والنسيجي وقد تم اعادة تقيم الكفائة المعلمات الخلوية لتسليط الضوء على الاخطاء الخلوية محليا وفي عالميا.النتائج: من مجموع 553 خزعة من الغدة الدرقية من ضمنها 81.6 ٪ حميدة و 18.4 ٪ خبيثة ، فقط 125 حالة اجري لها تشخيص خلوي قبل الجراحة والتشخيص النهائي النسيجية اللاحقة. من جميع الحالات ، كانت هناك حالاتان غير مقبولة، فقط 6 (4.9 ٪) تقارير خلوية لا تتطابق مع نتائجها النسيجية. وأظهرت 117 حالة المتبقية (95.1 ٪) اتفاق كامل بين اختباريين التقييم. تم التنبؤ بالخلايا الخبيثة بواسطة الفحص الخلوي في 82.9 ٪ مع دقة 94.3 ٪ وخصوصية 95.5 ٪. تم خزع جميع الحالات الستة التي لا تطابق الحالات الاخرى لها على نحو عشوائي دون وجود دليل صور، وكانت 4 حالات إيجابية كاذبة 3.2 ٪ معدل إيجابي كاذب و 89.2 ٪ قيمة تنبؤية إيجابية.الحالتين المتبقيتين 2 الغير مطابقة اعطتت نتيجة خلوية كاذبة بمعدل 1.6 ٪ سلبية كاذبة مع 97.7 ٪ القيمة التنبؤية السلبية. خلويًا ، ظهرت 5 مسحات (83.3٪) غير مطابقة ، حيث ان 4 مسحات منها إيجابية خاطئة و 1 سلبية خاطئة في مسحات الآفات الدرقية الغنية باللمفاويات ، وخاصة هاشيموتوس. خلل التشخيص الايبجابي الخاطء تالفت من حالتين وكانت في تشخيص لطاخات مفرطة الخلايا تظهر بعض ملامح سرطان حليمي، وإفراط في تقدير الخلايا الكبيرة مع عدم الاتساق النووي مثل سرطان جريبي و حالة تشخيص تضخم اللمفاوية مثل سرطان الغدد الليمفاوية. من ناحية أخرى ، فإن المسحات الخلوية المنخفضة ذات الخلايا اللمفاوية غير النمطية الغير الواضحة ناقص تشخيص سرطان الغدد الليمفاوية منخفضة الدرجة MALT ومعايير خلوية غير واضحة فشلت في تشخيص سرطان الورم الحليمي.الاستنتاجات: ينبغي تفسير مسحات الغدة الدرقية الغنية باللمفاويات من قبل أخصائيي الأمراض الخلوية ذوي الخبرة في سياق النتائج السريرية والإشعاعية والخلوية لأن مثل هذه الحالات قد تعطي بعض الاخطاء السيتومورفولوجية التي قد تقلل من مصداقيتها. في الحالات المشبوهة ، يجب تكرار الاختبارات الاخرى للتغلب على الاخطاء ومخاطر الميزات عند تطبيقها بمفردها.

Keywords

Pitfalls --- Thyroid Cytology --- Duhok --- Iraq.


Article
The value of fine needle aspiration cytology in the diagnosis of oral and jaw lesions in patients with plasma cell dyscrasias

Author: Bashar H. Abdullah بشار عبد الله
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2005 Volume: 17 Issue: 2 Pages: 41-44
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

Background: Fine needle aspiration cytology (FNAC) has been found to be very useful for the diagnosis of lesions as multiple myeloma (MM) and plasmacytomas in different parts of the body. The usefulness of such procedure has not yet been verified in the oral and maxillofacial region. This study was conducted to verify the value of FNAC in the diagnosis of oral and maxillofacial lesions in patients with plasma cell dyscrasias.Patients and methods: After clinical and radiological examination, FNAC was done by the use of 10 cc syringes with 22-23 gauge needles on 11 patients with lesions affecting the maxillofacial region. The smears stained with Leishman’s stain, to be examined microscopically.Results: Twenty eight patients were examined throughout 1.5 years period, 11 had soft tissue/or bone lesions affecting the maxillofacial region. 9 patients with MM and 2 had plasmacytoma. Of 9 patients with MM, the mandible was involved in 5 patients, 3 lesions affected the maxilla, and while in one patient the maxilla and mandible were both affected. Soft tissue lesions were seen in 6 patients. The results of FNAC showed that all lesions were due to involvement with myeloma cell infiltrates, with one exception of 2 osteolytic lesions which were due to odontogenic infection.Conclusion: FNAC is a very useful and safe procedure to diagnose different types of lesions affecting the oral cavity in patients with plasma cell dyscriasis

Listing 1 - 10 of 33 << page
of 4
>>
Sort by
Narrow your search

Resource type

article (33)


Language

English (31)

Arabic (1)

Arabic and English (1)


Year
From To Submit

2019 (1)

2018 (1)

2017 (6)

2016 (3)

2014 (3)

More...