research centers


Search results: Found 51

Listing 1 - 10 of 51 << page
of 6
>>
Sort by

Article
Antibacterial Activity Of Meropenem loaded to Chitosan Matrix
الفعالية الضد مايكروبية للنكومايسين والجنتا مايسين المتحررة من سبائك الكيتوسان والكيتوسان جلاتين

Author: Hanaa Jaafer jabaar AL –kabee هناء جعفر جبار الكعبي
Journal: Kufa Journal For Veterinary Medical Sciences مجلة الكوفة للعلوم الطبية البيطرية ISSN: 20779798 Year: 2014 Volume: 5 Issue: 1 Pages: 137-145
Publisher: University of Kufa جامعة الكوفة

Loading...
Loading...
Abstract

The antimicrobial activity of Meropenem released from chitosan matrix against gram positive and gram negative bacteria were studied. The inhibition zone diameter were determined After(24,48)hrs of incubation using agar diffusion assay . The results showed that both matrices were very active to deliver the antibiotic .there are significant increasing p<0.05 in inhibition zone after 48 hrs compared with 24 hrs of incubation. In 100 and 200mg of chitosan loaded with meropenem Also there is significant p<0.05 increasing in the antibiotic delivery in 200mg chitosan matrix. This study suggest to use such matrices in drug delivery system for local bioavailability of compound antibiotic against gram positive and gram negative bacteria at the same time which is very important in the treatment of some bacterial infections.

درست الفعالية الضد ميكروبية المتحررمن سبيكة الكيتوسان والكيتوسان جلاتين ضد البكتريا الموجبة والسالبة لصبغة غرام.استخدمت طريقة الانتشار بالاكار وحدد قطر منطقة التثبيط بعد (24و48) ساعة من الحضن . اظهرت النتائج ان كلا السبيكتين كانت فعالة في تحرر المضادات الحيوية . وظهرت زيادة معنوية في تحرركلا المضادين الحيويين ضمن مستوى احتمال p < 0.05 بعد 48 ساعة من الحضن مقارنة ب 24 ساعة من الحضن. ولوحظ وجود زيادة غير معنوية في تحرر المضادين في سبيكة الكيتوسان جلاتين. تقترح هده الدراسة استخدام مثل هده السبائك في انظمة التحرر الدوائي للوفرة الحيويةالموقعية (الموجهة) للمضادات الحيوية المركبة ضد البكتريا السالبة والموجبة لصبغة غرام والتي تكون مهمة في معالجة بعض الاصابات البكتيرية.

Keywords

meropenem --- chitosan.


Article
LOCALLY MADE CHITOSAN COVALENTLY LINKED TO INDOMETHACIN: A NOVEL APPROACH TO FORMULATE SUSTAINED RELEASE INDOMETHACIN CAPSULE IN BASRAH
الكايتوسان المصنع محليا" والمرتبط تساهميا" الى الاندوميثاسين: طريقة مبتكرة لتصنيع كبسول الاندوميتاسين ذو التحرير المستمر في البصرة

Loading...
Loading...
Abstract

ABSTRACTThe sustained release capsules are pharmaceutical dosage form used for the treatment of chronic rather than acutediseases and are useful because of its convenience and less side effects. This work describes an attempt to formulateindomethacin sustained release capsule using indomethacin covalently linked to chitosan. Chitosan was prepared byalkaline -N-deacetylation of chitin, which was isolated from the shells of local shrimps. The yield of chitosan wasfound to be 80%. This polymer was found to be white crystal, odorless and the IR spectroscopy of it was similar to astandard reference. Chitosan has been covalently linked to indomethacin and the ratio of drug to polymer was foundto be 5:1. The chemical interaction between the drug and the polymer was proved by IR studies. In vitro release atdifferent pH were performed and the release of indomethacin in the prepared sustained release capsule were testedand compared with conventional indomethacin capsule INDOMIN-25mg, and a commercial sustained release capsuleINDO-75-SR. The release of indomethacin in our product was completed within 12 hours, while the conventionalINDOMIN capsule and commercial INDO-75-SR capsule released within 2.5 and 5 hours respectively. In conclusion acheap, locally made chitosan can be used in formulation of sustained release capsules by covalently linked to drugscontaining carboxyl group (like indomethacin) or can be modified to have a carboxyl group.

فرع الادوية/كلية الطب/جامعة البصرةان محفظات (كبسولات) التحرير المستمر هي اشكال دوائية صلبة تستعمل لمعالجة الحالات المزمنة بدلا" من الحادة لأنها ملائمة وقليلة التاثيرات الجانبية. هذه الدراسة تمثل محاولة لاعداد محفظة للتحرير المستمر باستعمال الإندوميثاسين المرتبط بواسطة اّصرة تساهمية مع الكايتوسان. تم تحضير الكايتوسان بواسطة عملية النزع القلوي لأستيل الكايتين والذي تم عزله من قشور الربيان المحلي ووجد ان حصيلة الكايتوسان كانت ثمانون بالمائة (80%). هذا المكثور (البوليمر) كان ابيضا" وبلوريا" وعديم الرائحة وطيفه بالأاشعة تحت الحمراء يشبه مرجعا" قياسيا". لقد تم ربط الكايتوسان بالإندوميثاسين بواسطة الآصرة التساهمية ووجد ان نسبة الدواء للمكثور هي واحد إلى خمسة (1:5). هذه النسبة لوحظت بعد تكسير الأواصر التساهمية بين الكايتوسان والإندوميثاسين حيث وجد ان الإندوميثاسين يشكل نسبة (18%) وزن/وزن من مقترن الكايتوسان والإندوميثاسين وتم التثبت من التداخل الكيميائي بين العقار والمكثور بواسطة الدراسات الطيفية تحت الحمراء. كما أجريت دراسات التحرير الدوائي خارج الجسم عند مختلف درجات الباء هاء (دالة الحامضية). كما تم إختبار تحرير الإندوميثاسين من محفظة التحرير المستمر المحضرة ومقارنتها مع محفظة الإندوميثاسين الاعتيادية إندومين-25 ملغم (الحكمة-الأردن) ومع محفظة التحرير المستمر التجارية إندو-75- ت م (ش م ب–سوريا) فاستكمل تحرير الإندوميثاسين مع مستحضرنا خلال 12 ساعة بينما كان تحريره من محفظة الأندومين الاعتيادية ومحفظة الأندو 75 – ت م التجارية هو 2.5 و 5 ساعات على التوالي. واستنتاجا لذلك فأن الكايتوسن المحضر محليا والرخيص الثمن يمكن إستعماله في تحضير المحفظات ذات التحرير المستمر بواسطة الترابط بالاواصر التساهمية مع الادوية الحاوية على مجموعة الكاربوكسيل والادوية الاخرى التي يمكن تحويرها لتحتوي على مجموعة الكاربوكسيل. المجلة الطبية لجامعة البصرة ص6-14


Article
EFFECT OF CHITOSAN SHEETS ON WOUND HEALING
تاثير رقائق الكايتوسان على شفاء الجروح

Loading...
Loading...
Abstract

The study was aimed to evaluate the effect ofchitosan sheets on wound healing process andits activity as a wound dressing materials. Accordingly chitosan sheet was isolated and preparedfrom the exoskeleton of the native shrimps in Basrah Province. Twenty-four male rabbits wereused and two full-thickness circular cuts (2cm in diameter) were made on the dorsal aspect ofeach rabbit. The healing process was evaluated macroscopically by evaluation of the properties ofchitosan sheets on wound in terms of (adherence, absorption, and fluid accumulation) on differentintervals (i.e.lSt,3’d,7‘h,and 15"‘ post wounding day). On the other hand the contraction rate in testedliand control wounds were evaluated during different intervals (i.e. at lS‘,3' ,7‘ ,and I5“ post woundingday).The healing process was evaluated microscopically in terms of (infiltration of neutrophils andmacrophage infiltration, new blood vessels and fibroblast proliferation and Re—epithelialization).. The result of macroscopic evaluation showed that chitosan sheets were firmly adherent to' . the wound with underlying mild fluid accumulation during the first three post wounding day. At, the same time the sheets started to disappear and completely absorbed at 7' day after woundbreation. The result of the effect of the chitosan sheet on wound contraction demonstrated that thebontraction rate of tested wounds was significantly higher than in control wounds through thelperiod of experiment. In the treated group complete wound closure with contraction rate of 100%was reached at 103‘ post wounding day while the control wounds failed to close completely till the’ lend of experiment at 15‘ day. The results of microscopic evaluation of wound healing process weredemonstrated significant increase (p<0.05) infiltration of neutrophils in test wound at the first post‘wounding day then decrease and completely disappear while persist in control wound. Infiltrationof macrophages significantly increased (p<0.05) in tested wound during the period of experiment.§The fibrovascular granulation tissue and Re-epithelialization significantly more obvious in testediwound than in control wound through the period of experiment (p<0.050).

Keywords

Chitosan --- Epithelization --- wounds


Article
EFFECT OF CHITOSAN SHEET ON INFLAMMATION IN RABBITS
تاثير رقائق الكيتوسان على العملية الالتهابية في الارانب

Loading...
Loading...
Abstract

This study was conducted to investigate the effect of chitosan sheets onA inflammation. Accordingly chitosan sheet was isolated and prepared from theexoskeleton of the native shrimps in Basrah Province. The inflammation was induced andevaluated by polyvinyl sponge to determine the events which occur during theinflammatory process during different intervals in the presence or absence of chitosan‘ sheets .For this purpose 24 male rabbits were used and two linear skin incisions (2 cm)were made on the dorsal aspect in both sides of each rabbit. The inflammatory process atl,3,7&l5 day post wounding was evaluated macroscopically in terms of hyperemia,suppuration, dryness, and adhesion of margins and microscopically in terms of infiltrationof inflammatory cells specially neutrophils and macrophages, fibrovascular granulationtissue. _. The ‘results of macroscopic evaluation revealed that '-It first post wounding day thetest wound was significantly (p<0.05) more hyperemic than control wounds followed bya significant decrease in the severity of hyperemia in test wounds at ls‘, 3rd, 7"‘, and 15"‘post wounding days (p<0.05). The test wounds was more dry with less suppuration to_ words the end of the experiment than control wounds (p<0.05). The adhesive contactbetween the margin of the wounds was significantly higher in test wounds than in controlwounds through out the period of experiment (p<0.05).EFFECT OF CHITOSAN SHEET ON INFLAMMATION INRABBITSA Ala Al-Deen H. J awad*, J asim M. Al-Diab** and ManalKh.Ibraheem****Department of physiology,College of Veterinary Medicine, University of Basrah,Basrah,Iraq.**Department of pathology,College of Medicine, University of Basrah,Basrah,Iraq.‘ ' ***College of Pharmacy, University of Basrah,Basrah,lraq. ‘(Received 22 November 2005,Accepted 13 January 2006)Kcywords;glnflammation,Chitosan,hyperemic.. ABSTRACTThis study was conducted to investigate the effect of chitosan sheets onA inflammation. Accordingly chitosan sheet was isolated and prepared from theexoskeleton of the native shrimps in Basrah Province. The inflammation was induced andevaluated by polyvinyl sponge to determine the events which occur during theinflammatory process during different intervals in the presence or absence of chitosan‘ sheets .For this purpose 24 male rabbits were used and two linear skin incisions (2 cm)were made on the dorsal aspect in both sides of each rabbit. The inflammatory process atl,3,7&l5 day post wounding was evaluated macroscopically in terms of hyperemia,suppuration, dryness, and adhesion of margins and microscopically in terms of infiltrationof inflammatory cells specially neutrophils and macrophages, fibrovascular granulationtissue. _. The ‘results of macroscopic evaluation revealed that '-It first post wounding day thetest wound was significantly (p<0.05) more hyperemic than control wounds followed bya significant decrease in the severity of hyperemia in test wounds at ls‘, 3rd, 7"‘, and 15"‘post wounding days (p<0.05). The test wounds was more dry with less suppuration to_ words the end of the experiment than control wounds (p<0.05). The adhesive contactbetween the margin of the wounds was significantly higher in test wounds than in controlwounds through out the period of experiment (p<0.05).

Keywords

glnflammation --- Chitosan --- hyperemic


Article
GENOTOXICITY OF DIAZINON IN MALE ALBINO RATS FED ON DIET SUPPLEMENT WITH CHITOSAN

Author: Jihad A. Ahmed* Mohammed J. Alwan
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: Print:18138497 E; 24108456 Year: 2014 Volume: 13 Issue: 2 Pages: 151-163
Publisher: Basrah University جامعة البصرة

Loading...
Loading...
Abstract

In order to determine the genotoxic effects of diazinon and the role of chitosan to neutralize these effects, our study performed in (24) male rats(Rattus norvegicus) were divided into four groups and treated for (60) days as following, group (A) treated with normal saline and served as control, group (B) treated with [(1/10LD50) 3.8mg/kg. bw] of diazinon, group (C) treated with [(1/10LD50) 3.8mg/kg. bw] of diazinon and fed on diet supplement containing (1gram/1kg ration) chitosan, group (D) fed on diet supplement containing (1gram/1kg ration) chitosan only. The genotoxic effect of diazinon was evaluated by using the micronucleus assay showed increasing of micronucleated polychromatic erythrocytes were (11.6%) ingroup B, while (7%) in group C . The chromosomal aberration showed increase of presence of chromosomal aberration in group B was (7.5±1.04), while in the group C showed mild elevation in (3.25±0.8). The polymorphism of GSTM1 and GSTT1 genes showed highly incidence of both genes polymorphism in group B was (66.6%) while group C was (50%) . we concluded that diazinon is genotoxic pesticide and chitosan ameliorate it effects.


Article
EVALUATION OF THE ROLE OF CHITOSAN IN THE IMMUNOPATHOLOGY OF OSTEOMYELITIS IN DIABETIC RABBITS
تقییم دور الكایتوسان في المناعة المرضیة لألتھاب العظم في الأرانب المصابة بداء السكري

Author: Zainab Abdulkareem Maktoof* , Jihad Abdulameer Ahmed زینب عبد الكریم مكطوف* ,جھاد عبد الأمیر أحم
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: Print:18138497 E; 24108456 Year: 2018 Volume: 17 Issue: 2 Pages: 51-69
Publisher: Basrah University جامعة البصرة

Loading...
Loading...
Abstract

This study was conducted to investigate the beneficial effects of chitosan in theimmunopathology of osteomyelitis in diabetic rabbits; therefore, the experimentaldesign was carried out on 40 rabbits. They were divided into 5 groups each of 8animals, diabetes mellitus was induced in rabbits , then infected by Staphylococcusaureus and treated as following: First group (G1) was induced diabetes mellitus thenimmunized by whole sonicated S. aureus antigens (WSSAG) and inducedexperimentally osteomyelitis. The second group (G2) was induced diabetes mellitus,then immunized by (WSSAG) and induced experimentally osteomyelitis and fed ondiet containing chitosan. Third group (G3) was induced diabetes mellitus, and inducedexperimentally osteomyelitis only. Fourth group (G4) was induction of diabetesmellitus, and induced experimentally osteomyelitis and fed on diet containingchitosan. Fifth group (G5) was induced experimentally osteomyelitis only withoutdiabetes mellitus induction. Then at day 28th - 30th post immunization, skin test wasperformed to each of the immunized groups, and at day 30th the antibodies titer wasmeasured by passive hemagglutination assay and phagocytic activity, then the animalswere sacrificed and the treated bone taken for histopathological examination. In thepresent study , a significant increase was noted in the value of skin thickness of G2 at48 and 72 hrs PI.

ھذه الدراسة تمت للبحث في التأثیرات المفیدة لمادة الكایتوسان على المناعة المرضیة لألتھاب العظم التجریبيفي الأرانب المصابة بداء السكري، لذلك اقیمت التجربة على ٤٠أرنب .قسمت الأرانب الى خمس مجامیع ،٨حیوانات لكل مجموعة.تم أستحداث السكري في الأرانب ثم اصیبت بعدوى المكورات الذھبیة العنقودیة وتممعاملتھا كما یلي:المجموعة الاولى:تم استحداث السكري ثم تمنیعھا بواسطة المستضد الكلي المتكسر للمكورات الذھبیة العنقودیةوبعدھا تم اصابتھا بعدوى العظم التجریبیة. المجموعة الثانیة: تم استحداث السكري ثم تمنیعھا بواسطة المستضدالكلي المتكسر للمكورات الذھبیة العنقودیة وبعدھا تم اصابتھا بعدوى العظم التجریبیة وتمت معالجتھا بمادةالكایتوسان.المحموعة الثالثة:تم استحداث السكري واصابتھا بعدوى العظم التجریبیة.المجموعة الرابعة: تماستحداث السكري واصابتھا بعدوى العظم التجریبیة وتم علاجھا بمادة الكایتوسان. المجموعة الخامسة: تمتاستحداث اصابة العظم التجریبیة بدون اصابتھا بداء السكري.في الیوم ٣٠_٢٨تم عمل اختبار سمك الجلد في المجامیع الممنعة وفي الیوم ٣٠تم اختبار مستوى الاجسامالمضادة في الدم بواسطة اختبار التراص الدموي السلبي والفعالیة البلعمیة.ثم تمت التضحیة بالحیوانات و اخذتمقاطع من العظم المصاب للفحص النسیجي المرضي.ھذه الدراسة اظھرت زیادة معنویة في سمك الجلد في المجموعة الثانیة بعد ٧٢ساعة من الاصابة.كذلك زیادةمعنویة في مستوى الاجسام المضادة في المجموعة الثانیة. اظھرت النتائج ایضا ان المجموعة الثانیة استغرقتوقت اقل للتخلص من الكاربون في الدم.الدراسة النسیجیة المرضیة اظھرت ان في المجموعة الثانیة لاتوجد تغییرات مرضیة في سطح السمحاق اوالعظم اضافة الى وجود خلایا بانیة للعظم على سطح العظم.اما في المجامیع الاخرى كان ھناك تغییرات مثلوجود خلایا التھابیة وانقسام النسیج اللیفي في المجامیع الاولى ،الثالثة والرابعة.النتائج اظھرت ایضا تموت للعظم،نزف ، تجمع الخلایا الالتھابیة وتلیف في المجموعة الخامسة.بالاخذ بھذه النتائج ،اظھر الكایتوسان تأثیراتمفیدة على شفاء العظم في الارانب المصابة بالسكري بعد اصابتھا بعدوى المكورات الذھبیة العنقودیة

Keywords

Chitosan --- WSSAG --- Osteomyelitis


Article
Formulation and Evaluation of Acyclovir Microspheres
Formulation and Evaluation of Acyclovir Microspheres

Authors: Naresh K Naresh K --- Mounika K Mounika K --- Pavani S Pavani S
Journal: Iraqi Journal of Pharmaceutical Sciences المجلة العراقية للعلوم الصيدلانية ISSN: 16833597 Year: 2018 Volume: 27 Issue: 1 Pages: 1-7
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

The present study is to formulate and evaluate Acyclovir (ACV) microspheres using natural polymers like chitosan and sodium alginate. ACV is a DNA polymerase inhibitor used in treating herpes simplex virus infection and zoster varicella infections. Acyclovir is a suitable candidate for sustained-release (SR) administration as a result of its dosage regimen twice or thrice a day and relatively short plasma half-life (approximately 2 to 4 hours). Microspheres of ACV were prepared by an ionic dilution method using chitosan and sodium alginate as polymers. The prepared ACV microspheres were then subjected to FTIR, SEM, particle size, % yield, entrapment efficiency, in vitro dissolution studies and release kinetics mechanism. The FTIR spectra’s revealed that, there was no interaction between polymer and ACV. ACV microspheres were spherical in nature, which was confirmed by SEM. The particle size of microspheres was in the range of 23.8µm to 39.4µm. 72.9% drug entrapment efficiency was obtained in the formulation F3 (1:3 ratio) with a high concentration of calcium chloride (4% w/v). The in vitro performance of ACV microspheres showed sustained release depending on the polymer concentration and concentration of calcium chloride. The release data was best fitted with zero order kinetics and Korsemeyer-Peppas release mechanism and diffusion exponent ‘n’ value of was found to be Non-Fickian.

The present study is to formulate and evaluate Acyclovir (ACV) microspheres using natural polymers like chitosan and sodium alginate. ACV is a DNA polymerase inhibitor used in treating herpes simplex virus infection and zoster varicella infections. Acyclovir is a suitable candidate for sustained-release (SR) administration as a result of its dosage regimen twice or thrice a day and relatively short plasma half-life (approximately 2 to 4 hours). Microspheres of ACV were prepared by an ionic dilution method using chitosan and sodium alginate as polymers. The prepared ACV microspheres were then subjected to FTIR, SEM, particle size, % yield, entrapment efficiency, in vitro dissolution studies and release kinetics mechanism. The FTIR spectra’s revealed that, there was no interaction between polymer and ACV. ACV microspheres were spherical in nature, which was confirmed by SEM. The particle size of microspheres was in the range of 23.8µm to 39.4µm. 72.9% drug entrapment efficiency was obtained in the formulation F3 (1:3 ratio) with a high concentration of calcium chloride (4% w/v). The in vitro performance of ACV microspheres showed sustained release depending on the polymer concentration and concentration of calcium chloride. The release data was best fitted with zero order kinetics and Korsemeyer-Peppas release mechanism and diffusion exponent ‘n’ value of was found to be Non-Fickian.


Article
EFFECT OF CHITOSAN AND DEXTRIN COMBINATION ON EXPERIMENTALLY-INDUCED THERMAL INJURY IN RABBITS

Authors: Noor A.M. Hassan نور احمد محمد حسن --- Abdulkareem H. Abd عبد الكريم حميد عبد --- Bahaa F. Hussein بهاء فخري حسين
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2016 Volume: 14 Issue: 2 Pages: 125-130
Publisher: Al-Nahrain University جامعة النهرين

Loading...
Loading...
Abstract

Background:Burn is a major health problem, life threatening with a high mortality and morbidity; Chitosan and its formulations are used as a topical dressing in wounds and burns management due to its nontoxic, hemostatic, healing stimulant, antimicrobial, biocompatible and biodegradable properties as well as its vehicle use to deliver biopharmaceuticals, antimicrobials and growth factors into tissue.Objective:To evaluate the effects of chitosan-dextrin combination on induced burn in rabbits.Method:Forty domestic male rabbits, weighing 1250-1750 kg were divided into five groups, each of eight animals: AH group: apparently healthy rabbits, BWT group: left with no treatment, AR group: treated with Aqua Rosea, AG-S group: treated with silver sulfadiazine cream and CH-D group: treated with chitosan – dextrin combination; all animals (except AH group) wereinduced burn and treatedtopically on burned area once daily for 28days. Tissue levels ofvascular endothelial cell growth factor (VEGF), tumor necrosis factor alpha (TNF-α) and skin histological examination.Results: Histopathological evaluation showed enhances inflammatory response, vascularization, granulation tissue formation, and collage deposition due to the appropriate regulation of TNF-α and VEGF.Conclusion:Topical use of chitosan – dextrin combinationshowed effective and enhance wound healing activities.Key words:Thermal injury, burn, chitosan, dextrin.

Keywords

Thermal injury --- burn --- chitosan --- dextrin.


Article
Folated-nanocarrier for curcumin drug delivery in breast cancer therapy
حامل النانوي الفولیتي لتحویل داء الکورکومین في علاج سرطان الثدي

Author: Sharafaldin Al-musawi
Journal: Engineering and Technology Journal مجلة الهندسة والتكنولوجيا ISSN: 16816900 24120758 Year: 2015 Volume: 33 Issue: 9 Part (B) Scientific Pages: 1643-1654
Publisher: University of Technology الجامعة التكنولوجية

Loading...
Loading...
Abstract

Among the potent anticancer agents, curcumin(CU) has been found to be very efficacious against many different types of cancer cells. Chitosan-coated magnetic nanoparticles (CS MNPs) were prepared and utilized as a nano-carrier for loading of curcumin(FA-CS-CU-MNPs) througha reverse microemulsionmethod. This nanoformulation was evaluated against breast cancer cell lines in the in vitro conditions.Both shape and size properties were studied by zeta sizer, AFMand FESEM and the cell internalization ability of prepared nanoparticles was determined by fluorescence microscopy. It was found that the synthesized FA-CS-CU- MNPs were spherical in shape with an average size of 90±15 nm, low aggregation and good magnetic responsive properties. Meanwhile, the high drug loading efficiency (~73%) was remarkable. These FA-CS-CU- MNPs also demonstrated sustained release of CU at 37 ◦C in different buffer solutions. Afterwards the suitable dose and therapeutic effects of (FA-CS-CU- MNPs) for both breast cancer and normal cell lines were evaluated by MTT assay.The result showed that FA-CS-CU- MNPs retainedsignificant antitumor activities, no adverse effects was detected for normal cells.Additionally, it was observed that the FITC-labeled FA-CS-CU- MNPs could effectively enter into the cancer cells and induced cell apoptosis.

من بين العوامل القوية المضادة للسرطان، قد أثبت بأن مادة الكركمين (CU) تكون فعالة جدا ضد العديد من أنواع مختلفة من الخلايا السرطانية. قد أعدت و استعملت مغلفة الکايتوزان النانوية المغناطيسية (CS MNPs) باعتبارها الناقل النانوي لتحميل الكوركمين (FA-CS-CU- MNPs) من خلال طريقة مايکرو امولشن (microemulsion) العكسي. تم تقييم هذا الفرمول النانوي ضد خلايا سرطان الثدي في الظروف المختبرية (in vitro conditions). وقد درس كلا من الشکل و خصائص الحجم باستخدام أجهزة تحليل الحجم الحبيبي و مجهر الإلکتروني الماسح و أيضا تم تحديد قدرة استيعاب الخلية لدخول جسيمات متناهية الصغر بإستخدام مجهر الفلورسنتي. قد تبين أن المركب النانوي المصنع، قد يکون كروي الشكل، ذات حجم متوسط 15± 90 نانومتر، قليل التجمع و يملک خصائص مغناطيسية جيدة وفي الوقت نفسه، يملک كفائة عالية و ملحوظة لتحميل الأدوية (73٪). هذا الفرمول النانوي أيضا قد يحرر الدواء في C◦37 في محاليل بافرية مختلفة.بعد ذلك تم تقييم الجرعة المناسبة التأثيرات العلاجية لدي الجزيئية المرکبة بالدواء لكل من خلايا السرطانية للثدي و الخلايا العادية بإستخدام تقنية فحصassay MTT.و أظهرت النتائجبأن الجزيئية النانوية المرکبة بالدواءتملک تأثير ملحوظ في مجال الأنشطةالمضادة للورم، بحيث لم يتم الكشف عن أي آثار سلبية على الخلايا الطبيعية. بالإضافة إلى ذلك، لوحظ أن الجزيئية النانوية المرکبة بالدواءو مادة FITCتدخل بشكل فعال في الخلايا السرطانية و تسبب موت المبرمج لها.کلمات المرشدة: النظام النانوي، جزيئية النانوية، کرکومين، کايتوسان، سرطان.

Keywords

Cancer --- Chitosan --- Curcumin --- Nanoparticle --- Nanosystem


Article
Extraction of Chitosan from the shrimp shell and study physicochemical and functional properties
استخلاص الكايتوسان من قشور الروبيان ودراسة خواصه الفيزيوكيميائية والوظيفية

Loading...
Loading...
Abstract

The study included the preparation Chitosan extracted from shrimp shells ( Penaeus semisulcatus) by remove of acetyl groub from chitin, studied the physico-chemical and functional properties, which included (yield, moisture, ash, protein and solubility) which amounted to (15.0, 5.50, 0.20, 1.15 and 98.90)%, respectively and reached molecular weight (17.782)kDa,determination the degree of deacetylation of Chitosan by technology (FTIR) (98.50)%, as was the viscosity of Chitosan record (74.11) cP, as measured X-ray diffraction (XRD) Chitosan it was noted the presence of two sharp peaks at the corner (2θ) 9.50 and 20.08 degree, ,use the scanner electron microscope (SEM) to see morphological Chitosan record and the results showed the heterogeneity and lack of smoothness of the surface, while the profile of the thermal gravimetric analysis (TGA) for Chitosan record that there are two phases of thermal decomposition ,where the first phase began at 108.49ᵒ c while second phase at 308.56 ᵒc .

تم استخلاص الكايتوسان بالطريقة الكيميائية بازالة مجاميع الاستيل من الكايتين االمستخلص من قشور الروبيان بالاسم العلميPenaeus semisulcatus درست خواصه الفيزيوكيميائية والوظيفية التي تضمنت (الحاصل, الرطوبة, الرماد ,البروتين والذائبية ) والتي بلغت ( 15.0, 5.50, 0.20 , 1.15و 98.90) % على التوالي وبلغ الوزن الجزيئي 17.782)) كيلودالتون, اما درجة ازالة مجاميع الاستيل بتقنية FTIR فبلغت (98.50 )% فيماكانت لزوجته (74.11) سنتي بويز, كما قيس انحراف الاشعة السينية للكايتوسان المحضر ولوحظ وجود قمتين حادتين عند الزاوية (2θ) 9.50 و 20.08 درجة, أستعمل المجهر الالكتروني الماسح (SEM) لمعرفة مورفولوجي الكايتوسان , كما وجدت مرحلتين من التحلل الحراري الوزني حيث بدأت المرحلة الاولى عند 108.49ᵒم والمرحلة الثانية عند 308.56 ᵒم

Listing 1 - 10 of 51 << page
of 6
>>
Sort by
Narrow your search

Resource type

article (51)


Language

English (36)

Arabic and English (8)

Arabic (6)


Year
From To Submit

2019 (5)

2018 (6)

2017 (6)

2016 (11)

2015 (8)

More...