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Article
Effect of two types and two concentrations of cryoprotectants on ovine oocytes morphology and viability post-vitrification

Authors: Muhammad –Baqir M-R Fakhrildin --- Raghad H. A. Al-Moussawi.
Journal: Iraqi Journal of Embryos and Infertility Researches المجلة العراقية لبحوث الأجنة والعقم ISSN: eISSN: 26166984 / pISSN: 22180265 Year: 2013 Volume: 3 Issue: 6 Pages: 32-37
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background:Oocyte vitrificationisapromisingtechnique.Thechoiceofappropriatetypesandconcentrationsofcryoprotectants is essential for the success of the oocytes vitrification.Objective:This study aimed to investigate the effect of vitrificationonviabilityandmorphologyofoocytesandto compare the effect of several cryoprotectants on the viability and morphology of oocytes during vitrificationandpost-thawing.Methods:The sheep ovaries were collected from the local abattoir. Only normal and viable oocytes were included in this study. By using cryotop, immature oocytes that were viable with normal morphology were vitrifiedwith 15% DMSO and 15% EG supplemented with 0.0 M, 0.25M, or 0.5 M of either sucrose or trehalose as control and treated groups. Oocytes viability and morphology were assessed post-aspiration and post-thawing.Results:From the results of the present study, the percentage of post-thawing normal and viable oocyte reported with the use of 0.5M trehalose and EG in vitrificationsolution(VS)significantly)P<0.05)higherthanthepercentage of post-thawing normal and viable oocyte reported with use of 0.25 M trehalose and EG.Conclusion:Vitrificationissimpletechniqueandeasytoperformbutitneedssomeexperiencetopreventanyoocyte loss during vitrificationandthawingprocessing.Theuseof0.5Mofeithersucroseortrehalosein vitrificationsolutionimprovesthepercentageofpost-thawingviableandnormaloocytes.


Article
Effect of cryopreservation protoco1sin relation to human sperm parameters

Authors: Muhammad -Baqir M-R. Fukhrildin --- Anam R.AL-Salih --- Shaub Y. Hussain
Journal: Iraqi Journal of Embryos and Infertility Researches المجلة العراقية لبحوث الأجنة والعقم ISSN: eISSN: 26166984 / pISSN: 22180265 Year: 2014 Volume: 4 Issue: 1 Pages: 9-12
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Backgrou ndThe semen cryopreservation has an important role in the male fertility preservation. Certainly, patients who are prone to become infertile due to surgica l or medical treatments such as chemo- or radiotherapy for cancer treatment. In fact, semen cryostorage seems to be the only proven method that may offer these couples a chance of having children inthe future.Objecti vesThis study was aimed to investigate the effects of cryopreservat ion protocols on human sperm parameters. MethodsSixty semen samples with range of age (19-35) years were included in this study. Sperm parameters, were assessed pre- or post-cryopreservation. Cryopreservation protocols were done using SMART medium with either 15% dimethy l sulfoxide(DMSO) or 15% glycerol alone (as control group) or supplemented with either 0.25M or 0.5M of sucrose for treated groups. Crude data were statistica lly analyzedReo;ultsGenerally, the results of the present study showed that the sperm parameters were significantly deteriorated post-thawing (P<0.05) as compared to pre­ cryopreservation . Bothtreated groups (G2 and G3) showed best results as compared to G1 (control groups). In contrast, treated group (G2) with 0.25 M of sucrose appeared better results than the as G3 group that treated with 0.5M of sucroseConclusionSperm parameters including concentration, motility, and morphology were deteriorated after cryopreservat ion. Using 0.25M sucrose reduced the impact of cryopreservation.


Article
Cryopreservation of goat semen using Soy milk as alternative of egg yolk
تجميد السائل المنوي للماعز باستخدام حليب الصويا كبديل لصفار البيض

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Abstract

This study was carried out at Research Station, and laboratory, Animal Production Dept., Agric. College, Al-Muthanna Univ., during /2016 to 2017 to improve the quality of cryopreservation semen goats by identifying the best concentration of soy milk and comparing it to Egg yolk, besides glycerol 7% was used as a check treatment. The results showed a significant superiority( P≤0.05) for the TFCSM (Consists of tristtmen 3.63 g/100 ml, fructose 0.5 g/100 ml and citric acid 1.99 g/100 ml+5% soy milk) and difference in cry protectants results. G7% with TFCSM showed significant superiority( P≤0.05) in the percentage of progressive motility Type A, the non-progressive motility type D, Agglutination, live sperm with averages 18.88, 45.44, 3.86 and 76.50, respectively. The EG5% with TFCSM showed significant superiority( P≤0.05) in the percentage of progressive motility type A and B, the non-progressive motility type C and D and the percentage of normal sperm, with averages 17.76, 20.15, 15.82, 45.64 and 88.51, respectively.

اجريت هذه الدراسة في محطة الابحاث الثانية التابعة لكلية الزراعة/جامعة المثنى ومختبر الدراسات العليا التابع لقسم الانتاج الحيواني /كلية الزراعة/جامعة المثنى للمدة من2102016 ولغاية 9/7/2017 بهدف تحسين نوعية السائل المنوي المجمد للماعز الافغاني, لمقارنة مخفف حليب الصويا بمخفف صفار البيض التقليدي, للتأكد من إمكانية استبدال الجليسيرول بموانع تجميد ذات وزن جزيئي منخفض مثل الاثلين كلاي كول إذ استخدم الجليسيرول كمعاملة مقارنة بتركيز 7% واستخدم ثلاث تراكيز مختلفة من الاثلين كلاي كول (3, 5 و7% على التوالي) ومقارنة تداخل كل واحد من موانع التجميد على مخفف حليب الصويا وصفار البيض. وجد تفوق معنوي P≤0.05)) لمخفف الTFCSM (تكون من الTris 3.63غم/100مل, fructose 0.5غم/100 مل وcitric acid 1.99 غم/100مل+5% حليب الصويا) و بتباين نتائج موانع التجميد اذ اظهر ال%G7 مع الTFCSM تفوق معنويP≤0.05)) في النسبة المئوية للحركة التقدمية من النوع A, الحركة غير التقدمية من النوع D, تكتل النطف, النطف الحية اذ كانت المتوسطات 18.88, 45.44, 3.86 و 76.50 على التوالي. أما الEG5% مع الTFCSM فاظهر تفوقآ معنويآ P≤0.05)) في النسبة المئوية للحركة التقدمية من النوع A و B والحركة غير التقدمية من النوع C وD والنسبة المئوية للنطف الطبيعية اذ كانت المتوسطات 17.76, 20.15, 15.82, 45.64 و 88.51 على التوالي.


Article
Quality enhancement of cryopreserved spermatozoa of sutchi catfish (Pangasianodon hypophthalmus) with honey addition
تحسين جودة الحيوانات المنوية المجمدة لسمك تشوسي السلور باضافة العسل

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Abstract

Sutchi Catfish is one of the important fish commodities in Indonesia. Unfortunately, its seasonal spawning pattern causes limited supply. Cryopreservation is a solution to solve limited supply since it can store the spermatozoa in low temperature so that physiological, biological and morphological functions still remain. Improving the quality of cryopreservation is important to increase the success of Sutchi Catfish aquaculture. Adding honey in cryopreservation process is expected to increase the quality of spermatozoa since it contains with sugars as a source of spermatozoa’s energy. This study tried to compare the effectivity of honey in cryopreservation process with no addition. The treatments used in this study were T1 (0% honey), T2 (0.2% of honey), T3 (0.4% of honey), T4 (0.6% of honey) and T5 (0.8% of honey). 30 days after stored, the spermatozoa were checked their motility, viability, abnormality, fertility and hatching rate. This study showed that honey addition could increase the motility significantly (P<0.01) to 23.14% better than control. The viability increased significantly (P<0.01) to 23.17% better than control. The abnormality test did not show significant difference between honey addition and control although the abnormality value in control was the highest (10.75%). The fertilization rate increased significantly (P<0.01) to 28.85% better than control. The hatching rate increased significantly (P<0.01) to 29.78% better than control. The success of all test indicated that the addition of honey in cryopreservation process of spermatozoa could be performed on Sutchi Catfish to increase its production even though the limited spawning pattern.

تشوسيالسلور هو إحدى السلع السمكية الهامة في اندونيسيا. ولكن لديه الأنماط الإنجابية الموسمية التي تؤدي إلى محدوديته. والحفظ بالتبريد هو الحل للتعامل مع محدودية امدادات ذلك السمك لأنه يحفظ الحيوانات المنوية في درجات حرارة منخفضةٌ جدًّا حتّى يعمل الاحتفاظ الفسيولوجية والبيولوجية والمورفولوجية. يعتبر تحسين نوعية الحيوانات المنوية أمرا ضروريا لتحسين الزراعة الناجحة لتشوسي السلور. ومن المتوقع أن تحسن إضافة العسل في عملية الحفظ بالتبريد نوعية الحيوانات المنوية لأن العسل يحتوي على السكر الذي يستخدم لطاقة الحيوانات المنوية. يحاول هذا البحث على مقارنة فعالية إضافة العسل مع عدم إضافة العسل على عملية الحفظ بالتبريد. تمت الإضافة في هذه الدراسة T1 (0٪ العسل)، T2 (0,2٪ العسل)، T3 (0,4٪ العسل)، T4 (0,6٪ العسل)، T5(0,8٪ العسل). بعد ثلاثين يوما من حفظ، تتم مراقبة الحيوانات المنوية على الحركة، والسلامة، والشذوذ، والتسميد ونسبة الفقس. وتشير هذه الدراسة إلى نتيجة إضافة العسل في تحسين القدرة على الحركة بشكل ملحوظ (ف <0,01) ليصبح 23,14% أفضل من مجموعة السيطرة. وتزيد السلامة بشكل ملحوظ (ف <0,01) ليصبح 23,17 ٪ أفضل من مجموعة السيطرة. ولم يظهر اختبار الشذوذ فرقا كبيرا بين إضافة العسل ومجموعة السيطرة بالرغم من قيمة الشذوذ في عنصر التحكم هي الأعلى (10,75٪). يزيد التسميد بشكل كبير (ف <0,01) ليصبح 28,85 ٪ أفضل من مجموعة السيطرة. وتزيد نسبة القفس بشكل ملحوظ (ف <0,01) ليصبح 29,78 ٪ أفضل من مجموعة السيطرة. ويشير نجاح جميع الاختبارات إلى أن إضافة العسل على عملية الحفظ بالتبريد يمكن تطبيقها في الحيوانات المنوية ليزيد إنتاج سمك تشوسي السلور رغم وجود أنماط التكاثر المحدودة.


Article
The Effects of different type of cryopreservation on DNA in testicular tissue of mice

Author: Hakeem JK
Journal: Muthanna Medical Journal مجلة المثنى الطبية ISSN: 2226146x Year: 2018 Volume: 5 Issue: 2 Pages: 115-134
Publisher: Al-Muthanna University جامعة المثنى

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Abstract

Cryopreservation is a process where cells or whole tissues are preserved by cooling to low sub-zero temperatures, such as (typically) -80°C or -196°C and this process use extensively in programs of in vitro fertilization (IVF). This study is an attempt to evaluate DNA fragmentation in the cell after cryopreservation/thawing cycle when using different types of cryoprotectant (CPA), sixty mature fertile male mice were used in the current study, the mean age of these mice were ten weeks. Fifteen of them were considered as control group and the rest (forty five) as cryopreserved group, this group was divided into three subgroup according to the type of cryoprotectant (glycerol, 1, 2 propanediol and dimethylsulfoxide), each subgroup composed from fifteen mice. The testes were cryopreserved for six weeks then DNA fragmentation assay was done by single cell gel electrophoresis (comet assay). Comet results of the present study showed a highly significant (P<0.0001) increase in DNA damage in the cryopreserved testis (33.26%, 38.8% and 30.6% represent cryoprotectant glycerol, 1, 2 propanediol and dimethylsulfoxide respectively) compared with control group (23.06%) after six weeks of cryopreservation. From the results of the present study, it was concluded that there was increase the levels of DNA fragmentation in the testicular tissue after cryopreservation /thawing cycle differs according to the type of cryoprotectant, Dimethylsulfoxide cryoprotectant provides good protection to the testicular tissue and DNA in cryopreservation /thawing cycle than glycerol and 1, 2 propanediol.


Article
Trehalose and ascorbic acid improves the Cryopreservation of umbilical Cord Blood hematopoietic stem Cells (CD34+) with low Concentrations of Dimethylsulfoxide
التريهالوز وحامض الاسكوربيك يحسنان الخزن بالتجميد لخلايا دم الحبل السري الجذعية + CD34 مع التراكيز القليلة لـ Dimethylsulfoxide

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Cryopreservation of umbilical cord hematopoietic stem cells (HSCs) is essential step in stem cell transplantation. TheDimethylsulfoxide (DMSO) mostly used as a cryoprotective agent, associated with toxic effects to stem cells. Inorder to minimize the effects of DMSO, low concentrations of DMSO with additional cryoprotectants should used. In thisstudy mononuclear cells (MNCs) isolated by ficoll from umbilical cord blood (UCB), which contain hematopoietic stemcells (CD34+), were used for cryopreservation.Cryopreservation of UCB-derived MNCs was done for period of one month by using uncontrolled-rate freezingtechnique at -196˚C in liquid nitrogen. Cryopreservation solution was used which consisted of minimum essentialmedium (MEM) and 20% fetal calf serum (FCS) supplemented with the cryoprotectant dimethylsulfoxide (DMSO) intwo concentrations 2.5% and 5% DMSO, lower than usually used 10%, with and without 25μg/ml trehalose or 80μg/mlascorbic acid to improve cryopreservation process. The addition of trehalose and ascorbic acid improved cryopreservationprocess in comparison with control. Addition of 5% DMSO alone and with additives showed a better result than 2.5%DMSO alone or with additives for cryopreserving CD34+ cells as indicated by immunocytochemistry.Washing out DMSO also affected the count and viability of MNCs. These results indicated that it could use lowconcentrations of DMSO in cryopreservation of HSCs by association with trehalose and ascorbic acid.

عادة Dimethylsulfoxide (DMSO) الخزن بالتجميد لخلايا الحبل السري الجذعية خطوة اساسية في نقل الخلايا الجذعية. اليستخدم تراكيز اقل DMSO يستخدم كعامل حماية من التجميد يرافقه بعض السمية للخلايا الجذعية. لاجل تقليل تأثير المنه مع مواد تحمي من التجميد. في هذه الدراسة تم عزل الخلايا وحدة النواة والحاوبة على الخلايا الجذعية باستخدام الفيكول لغرض˚ التجميد. تم النجميد لخلايا وحيدة النواة والمعزولة من دم الحبل السري لمدة شهر باستخدام تقنية التجميد الممباشر بدرجة - 196 م% و 20 % من سيرم جنين الابقار بالاضفة الى تراكيز اقل من 10 MEM بالنايتروجين السائل. يتألف محلول الخزن بالتجميد من وسطوهي 2.5 % و 5% مع و بدون 25 مايكروغرام/مل من التريهالوز أو 80 مايكروغرام/ مل من حامض الاسكوربيك لتحسين DMSO منعملية الخزن بالتجميد. ان اضافة التريهالوز وحامض الاسكوربيك حسن عملية التجميد بالقارنة بالسيطرة.لوحده او مع الاضافات اظهرت نتائج افضل من تركيز 2.5 % لوحده او مع الاضافات للخلايا الجذعية المخزونة DMSO % ان اضافة 5أثر على عدد وحيوية الخلايا الوحيدة النواة. هذه النتائج اكدت DMSO بالتجميد كما اثبت بتقنية الكيمياء المناعية. غسل الفي الخزن بالتجميد للخلايا الجذعية باضافة التريهالوز وحامض الاسكوربيك. DMSO امكانية استخدام تراكيز اقل من


Article
The effects of Trehalose on the semen cryopreservation of Awasse ram
تأثير استخدام الـTrehalose في تجميد السائل المنوي للكباش العواسي

Authors: H. K. J. Al-Saab حازم كسار جاسر الصعب --- A. A. Hubi عبد الكريم عبد الرضا هوبي
Journal: Al-Anbar Journal of Veterinary Sciences مجلة الانبار للعلوم البيطرية ISSN: 19996527 Year: 2013 Volume: 6 Issue: 1 Pages: 14-20
Publisher: University of Fallujah جامعة الفلوجة

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Abstract

The purpose of this study was to determine the influence of Trehalose added to the freezing media on the semen cytological parameters post-thawing (motility, viability, fertility). The experiments were done on 32 ejaculates collected by artificial vagina from four Awassi rams aged 2.5-3 years as 2 ejaculate/ ram/ week. After collecting, the samples were pooling and diluted (1:20) in medium based on Tris in which Trehalose were added (100 mM/ml) or in medium without Trehalose (control). The diluted semen was cooled at 4Cº, placed in fine 0.25 ml French straws and then stored in liquid nitrogen, Thawing of the straws were done to evaluated the semen and to know the viability of sperm with 1-6 hours after thawing. invitro fertilization (IVF) used to evaluated the cryopreservation sperms. The results indicated that, there were a significant effect (p<0.05) for Trehalose (100 mM/ml) on the motility of sperms after thawing, which were 33.13±0.91 and 10.63±1.48% for treatment and control group respectively. There was no effect for the Treatments (Trehalose 100 mM/ml) on the invitro fertilization of sperms after thawing, which were 15±2.00 and 16.31±2.99% for both groups respectively.

أُجريت هذه التجربة لغرض تحسين حيوية النطف للكباش بعد التجميد وقد استخدم 4 كباش من سلالة العواسي التركي، بأعمار تتراوح ما بين 2.5 –3.0 سنة، اجري تجميع السائل المنوي (Pooling) بعد عملية الجمع وتم تخفيفه بنسبة 1: 20 بمخفف الـ(Tris) ومن ثم تقسيمه إلى جزأين الجزء الأول اضيف إليه Trehalose بنسبة 100 ملي مول/ مل والجزء الثاني استخدم كمجموعة سيطرة بعد ذلك اجريت عملية تجميد السائل المنوي المخفف بالنايتروجين السائل. أُجريت عملية الإسالة وفحص الحركة الفردية للنطف وقابليتها على البقاء لأطول مدة (1، 2، 3، 4، 5 ولغاية 6 ساعات) في درجة حرارة 37°م بعد شهر وشهرين وثلاثة أشهر من إجراء عملية التجميد، ولغرض اختبار قابلية نطف الكباش المحفوظة بالتجميد بالمخففات السابقة على الإخصاب تم استخدامها بالإخصاب الخارجي. أظهرت النتائج تأثيراً معنوياً (P<0.05) للمعاملة في الحركة الفردية للنطف بعد التجميد إذ بلغت33.13 ± 0.91 و10.63 ± 1.48% لمجموعتي المعاملة والسيطرة على التوالي. فيما لم يظهر تأثير للمعاملة على نسبة الإخصاب الخارجي للنطف بعد التجميد إذ بلغت للمجموعتين أعلاه 15.00±2.00 و16.31± 2.99% على التوالي.


Article
EXTRACTIUON AND LYOPHILIZATION OF THE LOW DENSITY OF LIPOPROTEIN INSTEAD OF EGG YOLK ON SOME SEMEN PARAMETERS OF AWASSI RAMS
استخلاص وتجفيد البروتينات الدهنية واطئة الكثافة واستخدامها محل صفار البيض في مخففات Tris وتأثيرها على بعض صفات النطف للكباش العواسي عند الحفظ بالتبريد على درجة حرارة 5م°

Authors: H. R. B. Al-Subaihawi حوراء رسن بردي الصبيحاوي --- H. K. J. Al-Saab حازم كسار جاسر الصعب
Journal: Iraqi Journal of Agricultural Science مجلة العلوم الزراعية العراقية ISSN: 00750530/24100862 Year: 2016 Volume: 47 Issue: 1 Pages: 350-360
Publisher: Baghdad University جامعة بغداد

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Abstract

This study was conducted to explain the possibility of lyophilization of low– density lipoprotein (LDL) that is extracted from the egg yolk to be used instead of the egg yolk in an extender semen that belong to the Awassi sheep and their effect on the characteristics of the semen after five days of cryopreservation, as well as to get low-density Lipoprotein, steriled and lyophilized (powder) lipoprotein that can be stored for a long time to used when needed. The study was conducted in the from July 2015 to February 2016. The first stage started from July until August 2015 the (LDL) was extracted from fresh egg. The second stage in August 2015. the liquid (LDL) has been lyophilized and turn into powder that is packed in vials, steriled vials and then was stored in 0°C. The third stage was conducted from January to February 2016 in the college of Agriculture/ University of Baghdad. the semen was collected from four of the Awassi rams of the age (2– 4) years and the samples was collected by using artificial vagina. After that the sample was divided on the experimental treatments evenly 1ml/ transaction by using extender Tris. The dilution ratio was 1: 10. The effect of the different concentrations had been studied of the LDL on the characteristic of semen and three concentration of the LDL were used (3.2, 4.8 and 6.2%) for the transactions T1, T2,T3 respectively and the control treatment (20% EY).The effect of the lyophilized LDL was studied on some characteristic of sperm when cryopreserved under the temperature 5°c for five days. The Results of the experiment to varying differences between transactions in sperm characteristics as treatment outperformed T3 (P <0.05) in the individual movement in the 2nd and 3rd days and did not notice a significant difference on the other days, while a significant difference in the percentage of live sperm among the treatments and control were observed viability of keeping LDL of sperm higher was than it is in the control treatment of the plasma membrane as treatment outperformed T2 and T3 (P <0.05) on the treatment of control on the 3rd, 4th and 5th of conservation was also excel themselves transactions in susceptibility saved for acrosome on different days of conservation compared with the control treatment as it overtook treatment T2 in the first two days and did not notice a significant difference on the 3rd and 4th among the treatments and control excelled treatments T2 and T3 (P <0.05) in 5th day of conservation to the control, we conclude from the foregoing that it is possible freeze-drying LDL and save the freeze and use it in time of need can also be used in lyophilized LDL thinners semen because of its positive impact in keeping sperm has been observed that the best concentration of sperm in maintaining the properties is 6.4% LDL compared with egg yolk and other transactions.

اجريت هذه الدراسة لغرض بيان امكانية تجفيد البروتينات الدهنية واطئة الكثافة(LDL) المستخلصة من صفار البيض واستخدامها بدل صفار البيض في مخفف السائل المنوي للأغنام العواسي وتأثيرها على صفات السائل المنوي عند الحفظ بالتبريد لمدة خمسة أيام فضلاَ عن الحصول على بروتينات دهنية واطئة الكثافة معقمة ومجفدة (مسحوق) يمكن خزنها لفترات طويلة واستخدامها عند الحاجة , اجريت هذه الدراسة للمدة من تموز 2015 الى شباط 2016 وهي على ثلاث مراحل, المرحلة الاولى من تموز الى اب 2015 وفيها تم استخلاص (LDL) من صفار البيض الطازج, المرحلة الثانية في اب 2015 وفيها تم تجفيد (LDL) السائل وتحويله الى مسحوق وتعبئته في علب معقمة وبعدها تم خزنه بالتجميد بدرجة صفر مْ, المرحلة الثالثة في كلية الزراعة/جامعة بغداد من كانون الثاني وحتى شباط تم جمع السائل المنوي من اربعة كباش عواسي باعمار تتراوح (2 -4) سنة تم فيها جمع عينات السائل المنوي بواسطة المهبل الاصطناعي, وقسمت العينة على معاملات التجربة بالتساوي 1مل/معاملة باستخدام مخفف Tris وكانت نسبة التخفيف 1:10 وتم دراسة تأثير التراكيز المختلفة لـ LDL على صفات السائل المنوي. وقد استخدم ثلاث تراكيزLDL (3.2 و4.8 و6.2 %) للمعاملاتT1 وT2 وT3 على التوالي والسيطرة (20% صفار البيض), تم دراسة تأثير LDL المجفد على بعض خصائص النطف عند الحفظ بالتبريد على درجة حرارة °5 م ولمدة خمسة أيام متتالية, اشارت نتائج التجربة الى تفاوت الفروق بين المعاملات في خصائص النطف إذ تفوقت المعاملة T3 معنويا (P<0.05)في الحركة الفردية في اليومين الثاني والثالث ولم نلاحظ فرق معنوي في الايام الاخرى للتبريد في حين لم يلاحظ فرق معنوي في النسبة المئوية للنطف الحية بين المعاملات والسيطرة وقد كانت قابلية حفظLDL للنطف اعلى مما هو عليه في معاملة السيطرة للغشاء البلازمي اذ تفوقت المعاملة T2 وT3 معنويا (P<0.05) على معاملة السيطرة في الأيام الثالث والرابع والخامس للحفظ كذلك كان التفوق للمعاملات نفسها في قابلية حفظها للأكروسوم في أيام مختلفة من الحفظ مقارنة مع معاملة السيطرة اذ تفوقت المعاملة T2 في اليومين الاول والثاني ولم نلاحظ فرق معنوي في اليوم الثالث والرابع بين المعاملات والسيطرة وتفوقت المعاملتين T2 وT3 معنويا (P<0.05) في اليوم الخامس من الحفظ على مجموعة السيطرة, نستنتج مما تقدم انه بالامكان تجفيد LDL وحفظه بالتجميد واستخدامه وقت الحاجة كما يمكن استخدام LDL المجفد في مخففات السائل المنوي لما له من تاثير ايجابي في حفظ النطف وقد لوحظ ان افضل تركيز في المحافظة على خصائص النطف هو 6.4% LDLمقارنة مع صفار البيض والمعاملات الاخرى.


Article
The Role of Honey Supplementation to Cryopreservation Solution on Human Sperm Parameters and DNA Integrity during Cryopreservation

Author: Rana A. R. Al-Saadi
Journal: Iraqi Journal of Embryos and Infertility Researches المجلة العراقية لبحوث الأجنة والعقم ISSN: eISSN: 26166984 / pISSN: 22180265 Year: 2016 Volume: 6 Issue: 1 Pages: 13-21
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background: Semen cryopreservation is a useful tool for preserving fertility for men involved in ART program who have been diagnosed with cancer and will undergo chemotherapy, radiotherapy or testicular surgery. Objective: The objective of this study to evaluate the effect of honey bee supplemented to cryoprotectant medium on post-thaw sperm DNA integrity ,morphology and round cells. Subjects ,Materials and Methods: Thirty semen samples were collected from 30 infertile patients. After assessment of semen analysis, semen samples were divided into 3 aliquots (0.7ml for each sample) and mixed with 1 ml of cryopreservation solution (G1,control) alone, enriched with 5% honey bee(G2) with 10% honey bee (G3) for cryopreservation. Current study used the acridine orange test to investigate the effect of honey bee in two concentration (5%, 10%) on the integrity of sperm chromatin structure pre- and post cryopreservation. Cryopreservation was done at -196 Co in liquid nitrogen and thawing was performed after six months. Direct swim up technique was used for in vitro sperm preparation post- thawing, sperm parameters were assessed and data were statistically analyzed pre- and post- thawing. Results: The results show there was a significant(P<0.05) increase in the percentage of morphologically normal sperm for all groups post-thawing, particularly for G3 group that was significantly (P<0.05) increased as compared to G1 and G2 groups, In contrast, non-significant differences (P>0.05) were observed between G1 and G2 groups. Sperm DNA fragmentation percentage (%) was significantly(P<0.05) increased in G1 post thawing without any additives as compared to pre-cryopreservation , where as in G2 and G3 sperm DNA fragmentation were increased but not significantly as post-cryopreservation groups. Also the results show that there were no significant differences between G2 and G3 whereas G3 gives better result as compared to G1 with significant differences. Round cells counts for all groups of post cryopreservation were significantly(P>0.05) decreased as compared to pre-cryopreservation. Non significant (P>0.05) differences in the round cells counts among all the groups of post -thawing in spite of G3 group has the lowest mean. Conclusions: From these results we concluded that supplementation of 10% of honey bee to the freezing cryoprotectant medium gives best protection to the morphology and DNA integrity of human sperm.


Article
Although Late; but the First, an Iraqi Success in Human Embryo Cryopreservation Using Vitrification and the Factors Affecting the Pregnancy Rate: Cross-Sectional Study

Authors: Thuraya H. Abdulla --- Ula M. Al-Kawaz --- Ali I. Rahim
Journal: Iraqi Journal of Embryos and Infertility Researches المجلة العراقية لبحوث الأجنة والعقم ISSN: eISSN: 26166984 / pISSN: 22180265 Year: 2018 Volume: 8 Issue: 1 Pages: 10-21
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background: it is important to study a history of the science to appreciate thepast as a motive for hard work in the present for better future. In the other hand,the factors affecting pregnancy rate of frozen embryos are yet to be clarified atdifferent embryonic developmental stages.Objectives: to record a brief history of embryo cryopreservation in Iraq and tostudy many clinical and embryological factors that might affect the pregnancyrate.Subjects, materials and Method: a cross-sectional study of many clinical and embryologicalvariable, where120 frozen-thawed embryo transfer cycles enrolled inthe study at the period from January 2017 till July 2018. The variables were statisticallyanalyzed first by one factor analysis comparing between pregnant andnon-pregnant cases; then by multivariate logistic regression analysis to illustratemain factors without a possible confounder effect.Results: In one factor analysis, the following variables showed a significantlyhigh effect on pregnancy rate (P value <0.001) which are women’s age, women’sweight, number of retrieved oocytes, mature oocytes and good quality embryos,developmental stage, E2 level, and endometrial thickness. While in multivariatelogistic regression analysis the women’s weight, the number of retrieved oocytesand good quality embryos were the main factors.Conclusions: it is judiciously to consider these factors while managing infertilecouples with embryo cryopreservation programs especially the modifiable factors.

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