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Molecular Study of Aeromonasspp. Isolated from Clinical and Environmental Samples

Author: Safaa M. Salman
Journal: journal of al-qadisiyah for pure science(quarterly) مجلة القادسية للعلوم الصرفة (فصلية). ISSN: 19972490 Year: 2015 Volume: 2 Issue: 20 Pages: 45-55
Publisher: Al-Qadisiyah University جامعة القادسية

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Abstract

Receved :7/4/2015 Accepted : 9/6/2015 and Azhar N. HussienAL-Qadisiya University / Collegeof Education / Biology DepartmentE-Mails: AbstractA total of 259 specimen from different clinical sources from patients of AL-Diwanyia'shospitals and environmental sources ( water) were collected. Biochemical and morphologicalcharacterization tests besides the use of API 20 E and Vitek System and Polymerase ChainReaction (PCR)Technique which used 16S rDNA geneshowed that seventeen isolates wereidentified as Aeromonas spp. These Isolates distributed as : 8 isolates belong to A. hydrophila, 4 isolates belong to A. sobria , 4 isolates belong to A. caviae and one isolate belong to A.salmonicida . PCR was used for detecting virulence factors such as exotoxin genes :hemolysin (hly A) , aerolysin(aer A) and cytolytic enterotoxin (aer) , results showed that77.7% and 62.5% of environmental and clinical isolates had hly A while 44.4 % and 50 %542015 ﺔﻨﺳ 2 ﺩﺪﻌﻟﺍ 20 ﺪﻠﺠﻤﻟﺍ ﺔﻓﺮﺼﻟﺍ ﻡﻮﻠﻌﻠﻟ ﺔﻴﺳﺩﺎﻘﻟﺍ ﺔﻠﺠﻣhad aer A , aer gene just found in clinical isolates in rate 25% while didn't found inenvironmental isolates .The same technique (PCR)was also used for detecting resistancegenes of beta - lactam antibiotic : blaCMY , blaTEM , blaCTX-M , and blaSHV genes which used inthis study . The results showed that 11.76% had blaCMYgene , 5.88% had blaTEM gene and17.64% had blaCTX-M gene of all isolates , while blaSHV gene didn't found in all the types ofisolates which isolated from clinical and environmental sources .

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