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Article
Immunohistochemical expression of D2-40, VEGF and PCNA as biological markers of lymphangiogenesis, angiogenesis and proliferation in pleomorphic adenoma of salivary gland origin

Authors: Hiba Jasim Rassol هبة جاسم رسول --- Kadhim Al-Soudani كاظم السوداني
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2013 Volume: 25 Issue: special issue 1 Pages: 53-58
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Pleomorphic adenoma is the most common benign salivary gland tumor and shows a pronouncedmorphological complexity and diversity; for this The immunoprofiles and clinical course of PA differed according tocellular differentiation. Therefore, it is important to assess potential biomarkers in diagnostic and therapeutic trials. Thisstudy evaluates the immunohistochemical expression of D2-40, VEGF and PCNA as markers of lymphangiogenesis,angiogenesis and proliferation of PA and their correlation with clinicpathological parameters and with each other.Materials and Methods: Twenty five formalin – fixed, paraffin – embedded tissue blocks were included in this study.After histopathological reassessment of haematoxylin & eosin stained sections for each block, animmunohistochemical staining was performed using anti D2-40, anti VEGF and anti PCNA monoclonal antibodies.Results: Positive immunohistochemical of D2-40, VEGF and PCNA was found in (100%), (92%) and (88%) of the casesrespectively. No statistically significant correlation neither among the markers immunoexpression nor with theclinicopathological finding, except a statistical significant correlation was found between (D2-40andVEGF)expression with the histopathological presentation of the cases.Conclusion: D2-40, VEGF and PCNA immunoexpression showed significant correlation with respect to thehistopathological presentation of the cases. While no significant correlation seen regarding the expression ofaforementioned markers with each other, suggests that each marker might affect on tumor behavior independently

Keywords

Pleomorphic adenoma --- D2-40 --- VEGF --- PCNA


Article
Assessment Of Vascular And Lymphatic Vessels Density In Benign Vascular Lesions Using CD34 And D2-40 Immunohistochemical Markers

Authors: Jawaher M. Tater --- Bashar H. Abdullah
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2017 Volume: 29 Issue: 2 Pages: 61-64
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Vascular tumors and malformations, comprising a broad category of lesions often referred to asvascular anomalies. Hemangioma, represents a variety of vascular lesions (both malformations and tumor), whilelobular capillary hemangioma is a common vascular lesion of the skin and mucous membranes that occurs mainly inchildren and young adults. Lymphangiomas are malformations of the lymphatic system. At the level of lightmicroscopy the small lymphatics vessels may be similar to capillaries and sometimes are only tentatively identified bythe nature of their contents or by immunohistochemical staining procedure. This study aimed to assess the vascularand lymphatic vessels density in benign vascular lesions using CD34 and D2-40 immunohistochemical markers.Materials and Methods: Twenty two formalin-fixed paraffin-embedded tissue blocks of Hemangioma/vascularmalformation, thirty of lobular capillary hemangioma and another twenty of lymphangioma.Results: Lymphatic vessel density expressed by D2-40 immunomarker was found in all cases with mean (24.01±14.74)in lymphangioma ,for lobular capillary hemangioma it was (12.67±6.66) and for hemangioma was (9.77±6.82)where as the mean of microvessel density count measured by CD34 immunomarker was (49.87±31.97) for lobularcapillary hemangioma , in hemangioma it was (37.42±23.40) and (25.90±12.23 ) for lymphangioma.Conclusions: All vascular lesions are a mixture of blood and lymphatic vessels with different proportions,hemangioma shows high percentage of blood vessels and lymphangioma shows high percentage of lymphaticvessels.


Article
Immunohistochemical Expression of MMP2, VEGF and D2-40 as Biological Markers of Local Invasion Potential, Angiogenesis and Lymphangiogenesis in Oral Squamous Cell Carcinoma and Verrucous Carcinoma

Authors: Roqaia G. Baqer رقية باقر --- Bashar H. Abdullah بشار عبد الله
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2016 Volume: 28 Issue: 3 Pages: 59-64
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Verrucous carcinoma (VSCC) is considered as a rare well differentiated carcinoma variant of SCC withno metastatic potential. The aim of this study is to evaluate the Immunohistochemical expression MMP-2, VEGF andD2-40 expression in OSCC and VSCC.Materials and methods: Thirty formalin fixed paraffin embedded tissue blocks of OSCC and another twelve VSCCwere collected and Four micrometer thick sections were cut from each block and mounted on positively chargedslides and stained immunohistochemically with monoclonal antibodies to MMP-2, VEGF and D2-40.Results: there is no statistical difference between SCC and VSCC regarding the immunoexpression of MMP-2 andVEGF. While the lymphatic vessels density were higher in SCC than VSCCConclusions: OSCC and VSCC bear similar local invasion and angiogenesis potentials when quantified with MMP-2and VEGF immunostaining respectively whereas OSCC have higher lymphatic vessels density than their VSCC asevaluated with D2-40 immunoexpression


Article
Lymphangiogenesis Using D2-40 in Oral Squamous Cell and Mucoepidermoid Carcinoma and its Correlation with Clinicopathological parameters

Author: Mustafa Gh. Taher
Journal: Diyala Journal of Medicine مجلة ديالى الطبية ISSN: 97642219 Year: 2014 Volume: 7 Issue: 1 Pages: 66-75
Publisher: Diyala University جامعة ديالى

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Abstract

Background: Oral squamous cell carcinoma (OSCC) and Mucoepidermoid carcinoma (MEC) are the most common malignant tumor of the oral cavity and salivary gland respectively, the extent of lymph node (LN) metastasis by lymphangiogenesis is a major determinant for the staging and the prognosis of these malignancies and often guides therapeutic decisions. Objective: To correlate the lymphangiogenesis of OSCC and MEC with their clinicopathological parameters. Material and Methods: Fifteen oral squamous cell carcinoma and eighteen mucoepidermoid carcinoma tissue samples collected during the period from 2008 till 2014. Age, gender, site of tumors and stage were reviewed. Lymphangiogenesis obtained by Immunohistochemical findings using D2-40 immunomarker. Results: All cases of OSCC and MEC exhibited positive immunostaining for lymphangiogenic marker. There is a correlation between OSCC and MEC regarding the age group and site of tumors (P=0.001), while gender, lymphatic vessel density (LVD) and TNM stage have no correlation with OSCC and MEC. Lymphatic vessel density and showed no relation with stage of OSCC and MEC.Conclusion: No statistical correlation was found between LVD which expressed by mean of lymphangiogenesis using D2-40 immunomarker and OSCC MEC stage.

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