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Article
Study in The Inhibitory Effect of Disinfectants and Antiseptic Traded From Local Market in Nineveh The Province of on Some Pathogenic Bacteria

Author: Bushra D. Hamad
Journal: JOURNAL OF EDUCATION AND SCIENCE مجلة التربية والعلم ISSN: 1812125X Year: 2019 Volume: 28 Issue: 2 Pages: 103-112
Publisher: Mosul University جامعة الموصل

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Abstract

This study deals with the comparison of the effect of some disinfectants used in Hospitals from local market of Nineveh province from different origins, on some Gram negative and Gram positive bacteria isolated from burns and wounds. These disinfectants includes : Dettol (septol) ,Hepatine , Povidine-iodine , Hand sterilizer all of them from local , Arabic and international origins. Results showed a clear resistance of the studied bacteria , and differences in bacterial sensitivity toward disinfectants depends on its origins and bacterial species .It was clear that the disinfectant (hepatine) was the best disinfectant in inhibiting growth , the danish origin was the best of them then Jordanian origin, the second disinfectant (dettol) of which the Jordanian origin was also the best, then (povidine-iodine) of which the Emirates origin was the best and then (hand sterilizer gel) of France origin was best.

Keywords

Antiseptic --- Disinfectants --- Hepatine


Article
Assessment of the Efficiency for some Disinfectants Against the Microorganisms Isolated From the Wards of Newborn and Premature Babies in Baghdad Teaching Hospital
تقييم كفاءة بعض المطهرات ضد الجراثيم العزولة من ردهات الاطفال الخدج وحديثي الولادة في مستشفى بغداد التعليمي

Author: yasamen H ali ياسمين حسن علي
Journal: nursing national Iraqi specility المجلة العراقية الوطنية للعلوم التمريضية ISSN: 18122388 Year: 2015 Volume: 28 Issue: 1 Pages: 112-121
Publisher: Baghdad University جامعة بغداد

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Abstract

Objective : To assess the efficiency for some disinfectants against the microorganisms isolated from the wards of newborn and premature babies in Educational Baghdad Hospital .Methodology :This study had done from 182014 untile 192014, we had selected three types of disinfectants ( Incidine , Bleach and Microbac Forte )which were used for disinfection in the wards of newborn babies at Educational Baghdad Hospital to assess their effect against the microorganisms isolated from these wards and study the mixed affect of these disinfectants againt same microorganisms .Results : The results of the present study showed that there is affect of the different concentrations of the used disinfectants against the microorganisms isolated from the wards of the newborn and premature babies , also there is a synergism affect of microbac forte with sodium hypochlorite and incidine , as well as , there is an antagonism affect of sodium hypochlorite with incidine a comparision if they used alone .Recommendation : The present study had been suggested to use the highly concentrations of Microbac forte , Incidine and sodium hypochlorite to sterilize the wards of newborn and premature babies , also the study had been suggested to use the mixed solutions of microbac forte with incidine and sodium hypochlorite to disinfect the same wards


Article
The effect of storage time and disinfection method on the activity of some dental stone disinfectants

Authors: Enas M. Tarik ايناس طارق --- Shatha S. Al-Ameer شذى الامير
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2005 Volume: 17 Issue: 3 Pages: 8-12
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: It has been recommended that all materials returning from the laboratory be disinfected before placement in the patient’s mouth. Various adverse reactions have been reported when using disinfectant solutions with impression materials. Therefore, disinfection of dental cast may be effective in preventing cross infection. This study was carried out to evaluate the effect of storage time on the antimicrobial activity of chlorhexidine, sodium hypochlorite, and madacide-1 solutions and to test the effect of spray, immersion and incorporation techniques on their activities.Materials and Methods: Stone specimens were prepared in cylindrical blocks, under aseptic conditions, containing the three tested solutions applied by spray, immersion and incorporation methods. These specimens were stored for (1½h, 1 day, 3days, 7days, and 10days) and then tested by using disk-diffusion plate method to estimate the release of disinfectant solutions against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa,and Candida albicans. The zones of inhibition were measured with a ruler (in mm) after incubation, which was for 24h at 37°C aerobically.Results: The results showed that all microorganisms were sensitive to chlorhexidine and sodium hypochlorite while E.coli and P. aeruginosa were resistant to madacide. Also it was shown that chlorhexidine was more effective than madacide and sodium hypochlorite as a long acting disinfectant while sodium hypochlorite was the most effective as a short acting one.Conclusion: Immersion method had more inhibitory effect than the other methods representing (60 %) of all the tested specimens followed by incorporation method (25%) and the least percentage for spray method (15%).


Article
The effect of addition of calcium hypochlorite disinfectant on some physical and mechanical properties of dental stone

Authors: Shorouq M. Abass شروق عباس --- Ibrahim K. Ibrahim
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2012 Volume: 24 Issue: special issue 1 Pages: 36-43
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: The potentially damaging effects of the immersion technique, the difficulty in covering the entiresurface of the cast with the spray disinfecting solution, and the inability to assume that every impression presented tothe laboratory has been disinfected has led to the need for incorporating a disinfectant directly into the calciumsulfate hemihydrate. This study was designed to evaluate the effect of the addition of Calcium Hypochoritedisinfectant on some physical and mechanical properties of the dental stone.Materials and methods: Calcium Hypochlorite disinfectant in aqueous solution in different concentrations 0.5, 1.0, 1.5and 2.0% was added to type III dental stone. Setting time, setting expansion, reproduction of details, compatibilitywith impression materials and consistency are physical properties which were evaluated. The compressive strengthand surface hardness as mechanical properties were also determined.Results: The results of this study showed that the addition of 1 % of calcium hypochlorite improved some of thephysical and mechanical properties of dental stone (Setting Expansion, Compressive Strength and Surface Hardness)while its adversely affected the other properties (Setting Time)Conclusions: The 1 % of calcium hypochlorite disinfectant solution could be used to disinfect dental cast with theleast effect when compared with the other concentrations of calcium hypochlorite


Article
Isolation and identification of fungi from two hospitals in Baghdad city and effect of disinfectants on some fungi
عزل وتشخيص الفطريات من مستشفييين في مدينة بغداد وتاثير المطهرات على بعض الفطريات

Authors: Sadiq R. Mohammed صادق رحيم محمد --- Mona H. AL-Jibouri منى حمودي الجبوري
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2015 Volume: 56 Issue: 1C Pages: 673-682
Publisher: Baghdad University جامعة بغداد

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Abstract

Swabs from two hospitals at Baghdad city were collected from the period 28-October-2013 to 31-March-2014, to found out the prevalence and distribution of fungi. These isolates were collected from hospitals' environment and most of the items used for hospital work in order to detect the nosocomial pathogens persistent. All fungal isolates were identified depending on the morphological and microscopic examinations as well as biochemical tests. Out of 220 environmental samples, 146 fungal isolates was obtained, Aspergillus flavus was more frequency and occurrence 23.29% and 15.45% respectively, followed Aspergillus niger was 15.57% and 10.45% then Penecillium spp. was 13.27% and 8.64%. In Al -Yarmouk Teaching Hospital more places contamination was burns 75%, followed dialysis 72.72% then RCU units 69.23%, while more places contamination in Imam Ali General Hospital was wards 70%, followed burns and Kitchen 58.33% then operation hall 57.14%. Determining the minimum inhibitory concentration for five types of disinfectants which are used in hospitals, the MIC values of disinfectants against fungal isolate were Chlorhexidin 7.5%, Chloroxylenol 1.25%, Formaldehyde 1.25%, Povidine iodine 10% and Sodium hypochlorite 7.5%. Chloroxylenol had significantly the most effective compound on fungi, followed formaldehyde then Sodium hipochlorite. This study found that hospitals contain different fungal species and genus, antiseptics and disinfectants have an effect on the fungus if it used correctly and permanent change in the use of antiseptics and disinfectants to avoid resistance of fungi.

تم جمع عزلات فطرية بطريقة اخذ المسحات من مستشفيين في مدينة بغداد للفترة من 28-10-2013 الى 31-3-2014 للتعرف على مدى تلوثها وانتشار وتوزيع الفطريات فيها. تم اخذ العينات البيئية من اماكن مختلفة في المستشفى شملت معظم الوحدات والاجهزة الطبية, للكشف عن تواجد الفطريات التي تسبب عدوى المستشفيات. تم تشخيص الفطريات اعتمادا على المظهر الخارجي والفحص المجهري والاختبارات البايوكيميائية. تم الحصول على 146 عزلة فطرية مختلفة من 220 مسحة المجموع الكلي للعينات البيئية الماخوذة, كان Aspergillus flavus الاكثر ظهورا وترددا بنسبة 23.29% و 15.45% على التوالي, تليها Aspergillus niger بنسبة 15.57% و 10.45% ثم Penecillium spp. بنسبة 13.27% و 8.64%. في مستشفى اليرموك التعليمي كان اكثر الاماكن تلوثا هي وحدة الحروق 75% تليها وحدة غسيل الكلى 72.72% ثم وحدة الانعاش 69.23%, اما في مستشفى الامام علي العام فان الاكثر تلوثا كانت ردهات رقود المرضى 70%, تليها وحدة الحروق والمطبخ 58.33% ثم صالة العمليات 57.14%. كانت قيم التركيز المثبط الادنى Chlorhexidin 7.5% و Chloroxylenol 1.25% و Formaldehyde 1.25% و Povidine iodine 10% و Sodium hypochlorite 7.5%. وجدت هذه الدراسة ان المستشفيات تحتوي على انواع واجناس فطرية مختلفة, وان المطهرات لها تاثيرا على الفطريات فيما لو استخدمت بشكل صحيح, ويجب التغيير المستمر في نوعية المطهرات المستخدمة لتجنب مقاومة الفطريات لهذه المطهرات.


Article
The Effect of Disinfectant on the Microstructure of Dental Stone at Different Time Intervals

Author: Dr. Shorouq Majid Abass B.D.S., M.Sc.* د. شروق ماجد
Journal: MUSTANSIRIA DENTAL JOURNAL مجلة المستنصرية لطب الاسنان ISSN: 18138500 Year: 2007 Volume: 4 Issue: 2 Pages: 199-205
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Gypsum products have been considered to be among the most widely used model and die materials. Modifications of these materials by adding chemicals or salts result in a change in their structure. This study was designed to evaluate the effect of the addition of Calcium Hypochlorite disinfectant on the microstructure of dental stone.Calcium Hypochlorite disinfectant in aqueous solution in different concentrations (0.5, 1.0, 1.5 and 2.0%) was added to type III dental stone. The microstructure of the dental stone samples was examined under the microscope and then photo scoped, pictures were taken at different time intervals (immediately, after 10, 30 min., 1and 2 hours) for all the test groups.The results of this study showed that at different time intervals, from the beginning of mixing of dental stone with Calcium Hypochlorite disinfectant solution, deformation in the microstructure was produced (calcium sulfate dihydrate crystals).Microscopically appearance of dental stone samples mixed with Calcium Hypochlorite disinfectant solution revealed a deformation in size and shape of the calcium sulfate dihydrate crystals when compared with the control group.


Article
An Insight Into Bacterial Profile and Antimicrobial Susceptibility of Burn Wound Infections in Kerbala, Iraq

Authors: Zuhair Hameed Abboud --- Noor Hameed Al-Ghanimi --- Mohanad Mohsin Ahmed
Journal: Karbala Journal of Medicine مجلة كربلاء الطبية ISSN: 19905483 Year: 2014 Volume: 7 Issue: 2 Pages: 2023-2032
Publisher: Kerbala University جامعة كربلاء

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Abstract

background: There is paucity of data on bacterial profiles and antibiotic susceptibility patterns in burns care units in Kerbala, Iraq we sought to undertake this study.Aim of the study: is to have an insight into the bacteriological profile of buns wound infections and evaluate the antimicrobial susceptibility pattern of the organisms isolated to antibiotics and disinfectants.Materials and Methods: This study was conducted prospectively at the Burns Care Unit (BCU) in Al-Hussein Medical City, Holy Kerbala Province, Iraq. Wound swabs were obtained from consecutive 57 burns patients treated in BCU for the period from November 2012 to March 2013. Microbial isolates were identified based on standard microbiological techniques. Antimicrobial susceptibility test was done by Kirby Bauer disc diffusion method, whereas efficacy of 5 most commonly used disinfectant was evaluated by agar-diffusion method.Results and Discussion: A total of 76 isolates were recovered. Pseudomonas aeruginosa was found to be the most common isolate (53.94%), followed by E.cloacae (25%), E.coli (11.84%), K.Pneumoniae (3.94%), S.aureus (3.94%), A. baumannii (1.31%). Majority of the bacterial isolates were multiple-drug resistant. Generally, Imipenem was the most effective antimicrobial agents. The best disinfectant was Sekusept Forte® whereas the least effective disinfectants were Povidone-Iodine and Chloroxylenol®.Conclusions: Pseudomonas was the predominant cause of burns infections and majority of isolates were multiple-drug resistant. These indicate the need for strict hygienic measurement to protect the burns patients from opportunistic pathogens.


Article
Effect of disinfectant agents on certain physical and mechanical properties of type IV dental stone

Authors: Hanan Abdul- Rahman Khalaf حنان عبد الرحمن خلف --- Mithaq Radhi Mohammed ميثاق راضي محمد
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2014 Volume: 26 Issue: 1 Pages: 24-31
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Dental stone casts come into contact with impression materials and becomes susceptible to cross contamination from saliva and blood. This study was done to evaluate the physical and mechanical properties of dental stone type IV after treatments with various disinfecting agents and regimes (methods).Materials and Methods: Type IV dental stone and different types of disinfecting agents were used and divided into seven groups: G1: dental stone without disinfection (control group), G2: dental stone mixed with silver nitrate powder 0.5% , G3: dental stone mixed with silver nitrate powder 1%, G4: dental stone mixed with copper sulfate powder 0.5%, G5: dental stone mixed with copper sulfate powder 1% ,G6: dental stone immersed in propanol 70% and G7: dental stone immersed in ethanol 70%.Setting time, linear setting expansion, surface detail reproduction, compressive strength of type IV dental stone as well as compatibility with auto mixing addition silicone impression material were evaluated. The statistical analysis were conducted by ANOVA test followed by LSD test (p<0.05), also chi square test was used.Results: The compressive strength, linear setting expansion, surface detail reproduction and compatibility of stone specimens was affected to a higher extent by mixing with silver nitrate powder 1%, copper sulfate powder 1% while treating the stone specimens with the disinfecting powders at low concentrations as well as immersion of stone specimens in either ethanol or propanol for 15 minutes produce less effect on the previous tested properties. Conclusion: Silver nitrate 0.5%, copper sulfate 0.5% powders as well as 15 minutes immersion in 70% ethanol or 70% propanol did not promote adverse alterations in most of evaluated properties of type IV dental stone


Article
A Study of sensitive S. aureus, E.coli and Ps. aeruginosa for chlorhexidin and sodium hypochlorite in Ramadi Teaching Hospital
دراسة حساسية بكترياS.aureus و E.coli و Ps.aeruginosaالمعزولة من بيئة مستشفى الرمادي التعليمي لمطهريﱠ الكلورهيكسيدين وهايبوكلورات الصوديوم

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Abstract

420 Samples were collected from some environmental locations in Ramadi Teaching Hospital included Floors, Walls, Air Windows, Doors and Sinks, 41 Isolates for S. aureus, 24 Isolates for E.coli and 22 Isolates for Ps. aeruginosa were diagnosed and Sensitive of 12 isolates for these bacteria toward Chlorhexidine and Sodium Hypochlorite were tested through determination MICs and MBCs and killing Times by used Broth Dilution Method, Chlorhexidine were high efficacy from Sodium Hypochlorite toward S. aureus E. coli and Ps. aeruginosa, MICs were range between 4-256 µg/ml for Chlorhexidine and 128-3000 µg/ml for Sodium Hypochlorite, MBCs were range between 8-512 µg/ml for Chlorhexidine and 256-4000 µg/ml for Sodium Hypochlorite and Killing Time for three isolates after exposed to MBCs were range between 10-15 min for Chlorhexidine and between 20-35 for Sodium Hypochlorite, In the finally S.aureus bacteria was the high sensitively for both disinfectants followed by E. coli but Ps. aeruginosa was the high resistance for both disinfectants

جمعت 420 عينة من مواقع بيئية مختلفة في مستشفى الرمادي التعليمي شملت الأرضيات والجدران والهواء والأبواب والنوافذ والمغاسل شخصت منها 41 عزلة لبكتريا S.aureus و24 عزلة لبكترياE.coli و22 عزلة لبكتريا Ps.aeruginosaوأختبرت حساسية 12 عزلة منتخبة (أربع عزلات لكل نوع) من الأنواع الثلاث للبكتريا تجاه مطهريﱠ الكلورهيكسيدين وهايبوكلورات الصوديوم من خلال تحديد التراكيز المثبطة الدنياMinimum Inhibition Concentrations (MICs) والتراكيز القاتلة الدنياMinimum Bacericidal Concentrations (MBCs) من خلال استخدام طريقة التخفيف بالمرق Broth Dilution Method ثمﱠ حدد زمن القتل للمطهرين المستخدمين في الدراسة تجاه العزلات المنتخبة. بينت النتائج أن مطهر الكلورهيكسيدين (الهيبتين) كان أعلى كفاءة من هايبوكلورات الصوديوم (القاصر) تجاه بكتريا S.aureus،E.coli و aeruginosa Ps. ﺇذ تراوحت قيم MICs للهيبتين ما بين (4-256) مايكروغرام/ مل فيما تراوحت قيم MICs لهايبوكلورات الصوديوم ما بين (128-3000) مايكروغرام/ مل، كما أظهرت النتائج أن قيم MBCs للكلورهيكسيدين تراوحت ما بين (8-512) مايكروغرام/ مل فيما كانت قيم MBCs لهايبوكلورات الصوديوم قد تراوحت ما بين (256-4000) مايكروغرام/ مل، وسجلت النتائج ان زمن القتل للمطهرات الكيميائية المستخدمة تجاه ثلاث عزلات للبكتريا المنتخبة بعد تعريضها للتراكيز القاتلة الدنيا للمطهرين قد تراوح ما بين ما بين (10-15) دقيقة للكلورهيكسيدين وما بين (20-35) دقيقة تجاه هايبوكلورات الصوديوم كما بينت النتائج أن بكترياaureus .S كانت الأكثر حساسية تجاه كلا المطهرين تلتها E.coli فيما كانت بكتريا aeruginosa Ps. الأكثر مقاومة


Article
Isolation and diagnosis of Propionibacteriun acnes from individuals suffer from acne and determination of MIC for common disinfectants against it
عزل وتشخيص بكتريا Propionibacterium acnes من مرضى يعانون من حب الشباب وتحديد التركيز المثبط الأدنى لبعض المطهرات الشائعة الاستعمال ضدها

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Abstract

The study included 175 samples of individuals suffer from acne included 73 Comedones and 102 samples Pustules of 159 patients ,Samples were collected from patients auditors of the Advisory of Dermatology of the Kirkuk General Hospital and Azadi teaching Hospital, between the ages of (12-32) year. Scrubbed 52 isolation belonging to the bacterium Propionibacterium acnes after diagnostic tests, the results showed that the percentage of the presence of these bacteria were more in comedone than in pastule as 32 isolated them isolated from comedone and 20 isolates of pastule, studied the value of the concentration of minimum inhibitory antiseptic( MIC) for Dettol, iodine, minudes and Sekulyse, ranging value against bacterial isolates P.acnes between (1.25-2.5%) (1%), (0.00312-0.05%) and (0.00156-0.0125%), respectively.

شملت الدراسة 175 عينة من أشخاص يعانون من حب الشباب تضمنت 73 عينة فدام Comedones و102 عينة بثور Pustules من 159 مريضا, جمعت عينات من المرضى المراجعين لشعبة الاستشارية الجلدية لمستشفى كركوك العام ومستشفى آزادي التعليمي ,تراوحت أعمارهم بين (12-32) سنة. نقيت 52 عزلة عائدة لبكتريا Propionibacterium acnes بعد إجراء اختبارات التشخيصية عليها أظهرت النتائج بان نسبة مئوية لتواجد هذه البكتريا كانت اكثر في الفدام مما هو في البثور حيث ان 32 بنسبة عزلة منها عزلت من البثور بنسبة (61.5%) و20 عزلة من البثور وبنسبة ((38.5%, درست قيمة تركيز المثبط الأدنى MIC لمطهرات الديتول, الايودين, المينوداس والسكيولايس, حيث تراوحت قيمتها ضد عزلات بكتريا P.acnes بين (2.5-1.25%),(1%), (0.05-0.00312%) و(0.0125-0.00156%) على التوالي.

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