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Article
SYNERGISTIC EFFECTS OF ETHYLENE DIAMINE TETRA ACETIC ACID AND IMIPENEM AGAINST Pseudomonas aeruginosa WERE ISOLATED FROM CHEESE IN HILLA PROVINCE.
التأثیر التأزري لأثلین ثنائي امین رباعي حامض الخلیك ) (EDTAوالامبنیم ضد P. aeruginosa المعزولة من الجبن في مناطق مختلفة من مدینة الحلة

Author: Mohammed K. Wali محمد كاظم والي
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: Print:18138497 E; 24108456 Year: 2018 Volume: 17 Issue: 2 Pages: 229-238
Publisher: Basrah University جامعة البصرة

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Abstract

The study was performed to the study synergistic effect of Ethylene diamine tetra acetic acid(EDTA) and Imipenem against Pseudomonas aeruginosa were isolated from cheese at Hillaprovince. Cheese samples 70 were collected randomly from (Retail, supermarkets and dairyshops) in Hilla and were transported to the laboratory.The result revealed (45%) Pseudomonas aeruginous detecte were collected from cheese.These isolates were tested for disk diffusion method for susceptibility of imipenem. Eightisolates were resistant , and (84.3%) of Pseudomonas aeruginosa strain were sensitive (84.3%)to EDTA alone.Also, the result showed of Synergistic EDTA- IMP disc diffusion against (8) isolate ofPseudomonas aeruginosa revealed (87.5%) isolates were sensitive. However; the The resultshowed significant (P < 0.05) of susceptibility to (8) P. aeruginosa isolates to imipenem with andwithout EDTA. In conclusion, according to the present study can use oxidant agent inhibition ofgrowth bacteria specific P. aeruginosa such as ethylene diamene tetra acidic acid (EDTA) andcan used in preserving food specific sold cheese.

اجریت الدراسة الحالیة لدراسة تأثیر التأزري لأثلین ثنائي امین رباعي حامض الخلیك و الامبنیم ضد Pseudomonas aeruginosaالمعزولة من الجبن في مناطق مختلفة من مدینة الحلة. ٧٠عینة من الجبن اخذت بصورة مختلفة من الحلة)السوبر ماركت، المحلات والباعة المتجولین( ونقلت الى المختبر لغرض التحلیل.اثناء فترة البحث وجد ) ٣٢ (%٤٥من مستعمرات Pseudomonas aeruginosaعزلت من الجبن .وھذه العزلاتاختبرت بواسطة الاقراص الانتشار لغرض قیاس حساسیتھا الى الامبنیم ،بینت بأن ) (8عزلات مقاومة الى الامبنیم ، بینمااوضحت النتائج بان) ٢٧ (%٨٤.٣من Pseudomonas aeruginosaحساسة ب لأثلین ثنائي امین رباعي حامضالخلیك ).(EDTAایضا وضحت النتائج بأن تأزریة اثلین ثنائي امین رباعي حامض الخلیك والامبنیم ) (EDTA- IMPبواسطة اقراصالانتشار ضد ) (٨عزلات Psedumonas aeruginosaبینت (% ٨٧.٥) ٧كانت حساسة. الاستنتاجات ، وفقا للدراسةالحالیة یمكن استخدام تثبیط العامل المؤكسد للبكتیریا نمو محددة P. aeruginosaمثل اثلین ثنائي امین رباعي حامض الخلیك)(EDTAویمكن استخدامھا في الحفاظ على الجبن المباع بالأغذیة المحددة


Article
Response of Local rice cultivar to Zinc and Boron application
استجابة الرز المحلي لاضافات من الزنك و البورون

Authors: Akram O. Esmail اكرم عثمان اسماعيل --- Ghafoor A. Mam Rasul غفور احمد مام رسول --- Aram A. Mohammed أرام عباس محمد
Journal: Baghdad Science Journal مجلة بغداد للعلوم ISSN: 20788665 24117986 Year: 2011 Volume: 8 Issue: 2 Pages: 289-294
Publisher: Baghdad University جامعة بغداد

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Abstract

Five levels of Zn-EDTA fertilizer and foliar application of boron were used to study the local rice response through studying of some vegetative and reproductive growth characters, by conducting two field experiments at Kanipanka Agricultural Research Station during the summer season of 2004 by using RCBD with three replications. Significant differences were found in studied characters, there were increase in the number of days from seeding to 50% flowering (94.330-96.233) days, from 50% flowering to physiological maturity (37.50-38.28) days, plant height (82.50-91.423) cm and LAI (5.441-7.525). Reproductive characters such as number of grains panicle-1 (74.11-85.88), number of panicles m-2 (321.00-426.083), biological yield (8166.166-11082.600) kg ha-1 and yield which increased from 3101.333 to 3862.166 kg ha-1 as Zn level increased from fo to f5, but in exp. No 2 although final yield increased from 3100.333 to 3791.500 kg ha-1, but there was increasing in sterility% from 15 for fo to 27 for f5 with boron foliar application. Results of the study indicate to positive response of local rice cultivars to zinc application due to the lack in zinc availability in sulaimani region.

تم استخدام خمسة مستويات مختلفة من سماد الزنك المخلبي مع رشة مضافة من البورون بهدف دراسة و استجابة الرز المحلي عن طريق دراسة بعض صفات النمو الخضري و النمو الثمري و ذلك بتطبيق تجربتين حقليتين في موقع كانيبانكة خلال موسم الصيف 2004 باستخدام تصميم القطع العشوائية الكاملة و بثلاثة مكررات, تم الحصول على اختلافات معنوية في الصفات المدروسة في عدد الايام من الزراعة الى 50% تزهير من (94,33-96,33 ) يوم, و من 50% تزهير الى النضج الفسلجي من (037,5-58,28)يوم ,و ارتفاع النبات من (82,5-91,423)سم و دليل المساحة الورقية من (5,441 – 7,525), و صفات النمو الثمري مثل عدد الحبوب في الدالية (74,11-85,18)حبة/ دالية, عدد الداليات/ م2 (321=426,083)دالية/ م2 ,الحاصل البايولوجي من(8166,166- 11082,6)كغم/ هكتار , و الحاصل النهائي من(3101,333- 3862,166) كغم/ هكتار, مع ارتفاع مستويات الزنك المضافة, اما بالنسبة الى التجربة الثانية فبالرغم من ارتفاع معنوي في الحاصل النهائي من 3100,333 الى 003791,5 كغم/ هكتار و لكن هناك ارتفاع في النسبة المئوية لعدم الخصب من 15% الى 27%, النتائج تؤشر الى استجابة موجبة للرز المحلي لاضافة السماد الزنك المخلبي والى نقص في جاهزية عنصر الزنك في ترب منطقة السليمانية.

Keywords

EDTA --- Zn --- B --- local rice


Article
ROLE OF LEAF NUTRITION BY MANGANES AND BORON IN WATER STREES FOR MAIZE ( Zea mays L.) 1.Grain yield and water use efficiency.
دور التغذية الورقية بالمنغنيز والبورون في ظروف الإجهاد المائي لنبات الذرة الصفراء 1. صفات الحاصل وكفاءة استعمال الماء. Zea mays L.

Author: يوسف محمد أبو ضاحي*
Journal: Diyala Journal of Agricultural Sciences مجلة ديالى للعلوم الزراعية ISSN: 20739524 Year: 2013 Volume: 5 Issue: 1 Pages: 239-250
Publisher: Diyala University جامعة ديالى

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Abstract

This experiment was conducted in field of Diyala Agricultural directorin Baquba in spring season 2011 to study the water stress by using foliarapplication concentrations of each boron and Manganese on weight of1000seed, Grain yield, protein content and WUE.The experiment was designed as RCBD with three concentration of sprayingManganes (0,25,50)mgMn.L-1added as Mn-EDTA(13%Mn) and used threeconcentration of spraying Boron (0,2,4) mgB.L-1 used Boric acid (17.4%B) andthree period of irrigation after(25,50,75%) of available water . Folair fertilizerwere applied at three time during of plant growth, to use Maize cultivar 5012 .Results showed the following: weight of 1000seed, Grain yield, and WUEThe increased with the increase in Manganese and Boron concentrationsvarieties differ significantly between Manganese levels and water stress levelsin most of characters. weight of 1000seed increased with the interactionbetween water stress levels x Boron levels . Also the most of characters weresignificantly influenced by interaction between moisture levels x Manganeseand boron concentrations . The use of nutrient application can improve plantperformance under water stress and can be used as away to increase water useefficiency especially under water shortage conditions.

نفذت هذه الدراسة في حقل التجارب التابع لمشتل مديرية زراعة ديالى / بعقوبة خلالالموسم الربيعي 2011 بهدف إيجاد السبل الكفيلة لمواجهة شحة المياه التي يشهدها العراق وكثير مندول العالم باستخدام التغذية المعدنية وفهم بعض التأثيرات والتكيفات الفسلجية للجفاف . زرعت بذورالذرة الصفراء ( صنف 5012 ) . نفذت الدراسة وفق تصميم القطاعات العشوائية الكاملة بثلاثةلتر- 1 ترش . Mn مكررات اشتملت معاملات التجربة على رش المنغنيز بتراكيز 50، 25، 0 ملغموكذلك تم استخدام ثلاثة تراكيز للرش ( Mn % على النبات على شكل المنغنيز المخلبي ( 13وشد مائي ( B% لتر- 1 تضاف على شكل حامض البوريك ( 17.4 .B بالبورون هي 4، 2، 0 ملغممختلف بإضافة الماء عند استنزاف 75 ، 50 ، 25 % من الماء الجاهز. تم الرش بثلاث دفعات بعد80 ، 60 ، 40 يوما من الإنبات .دلت النتائج على ظهور تأثير معنوي في حالة الرش بالتراكيز العالية للمنغنيز و البورون أورشهما معا على وزن 1000 حبة وحاصل الحبوب وكفاءة استعمال الماء .تفوق مستوى التداخللتر- 1 ) ) على مستوى المقارنة لصفة 1000 حبة وحاصل الحبوب وكفاءة .Mn 50 ملغم + W3 )56.38 % على الترتيب . تفوق مستوى ، % 56.38 ، % استعمال الماء بنسبة زيادة 18.10لتر- 1 ) )على مستوى المقارنة لصفة 1000 حبة بنسبة زيادة 7.09 .B 4 ملغم + W التداخل( 3لتر- 1) على مستوى .B 4 ملغم + لتر- 1 .Mn 50 ملغم + W % .تفوق مستوى التداخل ( 371.80 % على الترتيب. ، % المقارنة لصفة 1000 حبة وكفاءة استعمال الماء بنسبة زيادة 26.90إن إضافة المغذيات بكميات مناسبة ممكن أن يحسن من أداء النبات تحت إجهاد رطوبي وممكن أنيستخدم كتقنية لزيادة تحمل النبات للعطش

Keywords

Mn-EDTA --- Boric acid --- WUE .


Article
Study of Some Physical and Chemical Properties of Staphylolysin Enzyme purified from Pseudomonas aeruginosa
دراسة بعض الخصائص الفيزيائية والكيميائية لأنزيم الستافيلولايسين Staphylolysin المنقى من بكترياالـ Pseudomonas aeruginosa

Authors: May T. Flaih مي طالب فليح --- Shayma'a S. Najim شيماء سهيل نجم
Journal: Baghdad Science Journal مجلة بغداد للعلوم ISSN: 20788665 24117986 Year: 2014 Volume: 11 Issue: 2 عدد خاص بالمؤتمر النسوي الثاني Pages: 819-828
Publisher: Baghdad University جامعة بغداد

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Abstract

Some of the characters of the Staphylolysin A and D enzymes purified from Pseudomonas aeruginosa P16 and P5 respectively were studied, the molecular weights of Staphylolysin A and D were 20.417 kilo dalton and 23.988 kilo Dalton respectively by SDS- polyacryl amide gel electrophoresis. The optimum pH for staphylolysin A activity was found to be 8 which gives higher activity reaches 150 unit/ml, and for enzyme stability was 7.5-8.5 in which the enzyme nearly retained its full activity, while it was 9.5 for staphylolysin D that gives higher activity of 16 unit/ml,and 8.5-9.5 for enzyme stability in which the enzyme nearly retained its full activity, Maximum activity of two enzymes was obtained at 40C in which the specific activity for staphylolysin A and D were 140 and 16.4 unit/ml, and the two enzymes remained approximately without change at 25-40C for one hour. When the effects of some materials on Staphylolysin A&D activity were studied, the results showed that both sodium chloride & potassium chloride at 1 & 5 mM had the activator effect on enzymatic activity compared with its control where the staphylolysin A and D retained 105% ,108% and 102%, 104% of their activity respectively when treated with sodium chloride, while they retained 110%, 114% and 133%, 118% of their activity respectively when treated with potassium chloride. The enzymatic activity for both enzymes were inhibited when treated with ferric , mercury and zinc chloride at variable ratios, Staphylolysin A kept 73% and 7% of its initial activity respectively when treated with 5mM of ferric chloride and mercury chloride respectively and it kept only 9% of its initial activity when treated with 0.1mM Zinc chloride . Staphylolysin D kept 45% and 13% of it is initial activity respectively when treated with 5mM of ferric chloride and mercury chloride respectively and it kept only 23% of its initial activity when treated with 0.1mM Zinc chloride while enzymatic activity for both enzymes were not affected when treated with EDTA at l0mM and phenyl methyl sulphonyl fluoride (PMSF) at 0.4mM.These results referred to that Staphylolysin A and D are Zn -metallo endopeptidase .

درست بعض خصائص انزيم الستافيلولايسينStaphylolysin )) A وD المنقى من بكترياP5, P16 Pseudomonas aeruginosa على التوالي فكان الوزن الجزيئي 20,417 كيلو دالتون و 23,988 كيلودالتون للستافيلولايسين D,A على التوالي عند تقديرهما بطريقة الترحيل الكهربائي في هلام SDS- PAGE. لوحظ ان الرقم الهيدروجيني الامثل لفعالية الستافيلولايسين A هو 8 اذا اعطى فعالية بلغت 150 وحدة / مل, ولثبات الانزيم 7.5-8.5 اذ احتفظ الانزيم بكامل فعاليته تقريبا, فيما كان الرقم الهيدروجيني الامثل لفعالية الستافيلولايسين D هو 9.5 إذ اعطى اعلى فعالية بلغت 16 وحدة /مل، ولثبات الانزيم 9.5-8.5 إذ احتفظ الانزيم بكامل فعاليته تقريباً، وظهرت اقصى فعالية للانزيمين عند درجة حرارة 40م اذ بلغت الفعالية النوعية للستافيلولايسن A 140 وحدة / مل وللستافيلولايسين 16.4 D وحدة/ مل , واحتفظ كلا الانزيمين بكامل فعاليتهما تقريبا عند حضنها بدرجة حرارة 25-40 م لمدة ساعة . عند دراسة تاثير بعض المواد في الفعالية الانزيمية الستافيلولايسين Staphylolysin )) D,A كان لكلوريد الصوديوم وكلوريد البوتاسيوم بتركيز1و5 ملي مولار تأثير منشط للفعالية الانزيمية قياسا بالسيطرة اذ احتفظ انزيم الستافيلولايسينD,A بـــ %105، %108 و 102%، 104% من فعاليتهما على التوالي عند معاملتهما بكلوريد الصوديوم بينما احتفظ كلا الانزيمين بـ 110%%114 , و133% ،118% من فعاليتهما على التوالي عند معاملتهما بكلوريد البوتاسيوم. ثبطت الفعالية الانزيمية لكلا الانزيمين عند معاملتهما بكلوريدات الحديد والزئبق والخارصين وبنسب متفاوتة، إذ احتفظ انزيم الستافيلولايسينA بـــ 73% و 7% من فعاليته الابتدائية على التوالي عند معاملته بـ 5 ملي مولار من كلوريد الحديد والزئبق على التوالي، و9% فقط من فعاليته عند معاملته بـ 0.1 ملي مولار من كلوريد الخارصين, فيما احتفظ انزيم الستافيلولايسين D بــــ 45% و 13% من فعاليته فقط عند معاملته بــ 5 ملي مولار من كلوريد الحديد والزئبق على التوالي، 23% فقط من فعاليته عند معاملته بـــ 0.1 ملي مولار من كلوريد الخارصين , بينما احتفظ كلا الانزيمين بكامل فعاليتهما عند المعاملة بمادة EDTA بتركيز 10 ملي مولار , وفينيل مثيل سلفونيل فلورايد (PMSF ) بتركيز 0.4 ملي مولار .هذه النتائج تشير الى ان انزيمي الستافيلولايسين من الانزيمات المعدنية (Zn -metallo endopeptidase) .


Article
The Antibacterial Effect of QMix, a Novel Root Canal Irrigant (Ex vivo Study)

Authors: Abdul Khaliq Q Al-Sheik Abidal --- Ghada Y Abdul-Rahman --- Ali W Tawfeeq
Journal: Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان ISSN: 18121217 Year: 2013 Volume: 13 Issue: 27 Pages: 537-546
Publisher: Mosul University جامعة الموصل

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Abstract

To evaluate the antibacterial effect of a novel root canal irrigant, QMix, by evaluating its effect against E. faecalis and comparing it to 17% EDTA and 2% Chlorhexidine digluconate. Materials and Methods: Minimum Inhibitory Concentration (MIC), Minimum bactericidal concentration (MBC) and direct exposure test were the techniques used. In the MIC and MBC technique, the irrigants (QMix, 17% EDTA, 2% CHX) were serially diluted in BHI broth and 0.2 mL of the tested bacterial suspensions was added. Results were obtained on the basis of turbidity and growth on agar plates. In the direct exposure test, Enterococcus faecalis were exposed to QMix, 2% Chlorhexidinedigluconate and 17% EDTA for 5 seconds, 30 seconds and 3 minutes. Following exposure, samples were taken and serially diluted and incubated anaerobically on E. faecalis selective media for 24 hours to count the resistance of the bacteria. Results: In the MIC and MBC technique, CHX showed to be more effective against E. faecalis than both QMix and EDTA, as lower dilutions were required to inhibit growth of both bacteria. Ethylene diamine tetra acid (EDTA) was the least effective. In the direct exposure test, QMix was more effective than CHX and EDTA as it was the only solution to be able to kill all bacteria. Few E. faecalis cells remained even after exposure of bacteria to 3 minutes of EDTA and CHX. There was significant difference between QMix and both EDTA and CHX in killing of E. feacalis at 5 seconds exposure (p<0.05). QMix killed more than 95% of bacteria, whereas CHX and EDTA killed fewer than 20% (p<0.05). There was no statistical significance between the irrigant solutions at 3 minutes of exposure. Conclusions: In the MIC and MBC techniques, Chlorhexidinedigluconate was found superior to both QMix and EDTA. In the Direct Exposure Test, QMix showed the best performance as it was the only irrigant solution to kill all E. faecalis cells, as well as killing more than 95% of all bacteria at 5 seconds exposure

Keywords

Anibacterial effect --- QMix --- EDTA --- CHX


Article
The effect of various endodontic irrigants on the sealing ability of Biodentine and other root perforation repair materials (In vitro study)

Authors: Ahmed K. Al-Zubaidi احمد الزبيدي --- Abdul Karim J. Al-Azzawi عبد الكريم جاسم العزاوي
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2014 Volume: 26 Issue: 3 Pages: 1-8
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: This in vitro study was carried out to evaluate the effect of various endodontic irrigants (sodiumhypochlorite, ethylene diaminetetracetic acid and normal saline) on sealing ability of (Biodentine, mineral trioxideaggregate, and amalgam) used to repair furcal perforations.Material and methods: One hundred and twenty extracted human molars with divergent roots were used in thisstudy. A standard root canal access cavity was prepared in each tooth and furcal perforation was made and wasstandardized by using k file size 100 instrument to get a perforation of (1.32mm) in diameter .The teeth were randomlydivided in to three groups of 40 teeth according to the type of material used to repair the perforations (Group A: Thefurcal perforations were repaired with Biodentine, Group B: The furcal perforations were repaired with MTA ,GroupC: The furcal perforations were repaired with Amalgam). Each group was then subdivided into 4 subgroupsaccording to irrigation regimens applied over the repair site (Subgroup 1: without irrigation, Subgroup 2: the pulpchamber was gently irrigated with 10 mL 5.25% Sodium hypochlorite for 10 minutes, Subgroup 3: Pulp chamber wasgently irrigated with 10 mL 17% Ethylene Diaminetetracetic acid for 10 minutes, Subgroup 4: pulp chamber wasgently irrigated with 10 mL normal saline for 10 minutes. Each tooth was coated with two layers of nail varnish andthen sticky wax except 1 to 2 mm around the perforation site. Each tooth was placed in glass vial containing 3 ml ofbuffered Methylene blue dye at (37°C, pH 7) and kept in an incubator for 72 hour at 100% humidity. After dyeapplication, the teeth were washed in running water for 5 min. Each tooth was sectioned longitudinally in abuccolingual direction.Results: The results showed that group A has least mean of dye penetration and the difference was highly significantwith group C and non-significant with group B.Saline and NaOCl increase the sealing of all groups while EDTAsignificantly increased the dye penetration of Biodentine and MTA respectively.Conclusions: Biodentine has the best sealing ability of the tested materials while amalgam showed the highest dyepenetration of all tested materials. Saline and NaOCl increase the sealing ability of Biodentine and MTA where asEDTA decreased the sealing efficacy of MTA and Biodentine

الھدف من ھذه الدراسة كان لتقییم تأثیر مواد مختلفة لغسل قنوات الجذور (محلول الاثیلین دایمین تیترا استك اسد , محلول الھایبوكلوراید و المحلول الملحي العادي ) على قدرة الختملمواد (البایودنتین , مادة تجمع المعدن ثلاثي الاوكسد و الاملكم ) المستعملة في ترمیم ثقب مفترق الجذور .استخدمت في ھذه الدراسة مئة و عشرون ضرس سفلیة حدیثة القلع , متباعدة الجذور .تم تحضیر قناه الجذر القیاسیة لكل سن ثم عمل انثقاب لمفترق الجذر و تم معایرتة بحیث یصبحمقیاس 100 , قسمت الاضراس عشوائیا الى ثلات مجموعات تحوي على اربعین رحى استنادا لنوع المادة المستعملة في ترمیم انثقاب k بقطر ( 1,32 ملم ) بواسطة استخدام مبردمفترق الجذر كالاتي :مجموعة أ : رممت انثقابات الجذور بمادة البایودنتین .مجموعة ب : رممت انثقابات الجذور بمادة تجمع المعدن ثلاثي الاوكسد .مجموعة ج : رممت انثقابات الجذور بمادة الاملغمثم تم تقسیم كل مجموعة إلى 4 مجموعات فرعیة وفقا لنظم الري التي تم تطبیقھا على موقع إصلاح مفترق الجذور على النحو التالي:المجموعة الفرعیة الاولى : لم یجري أي ري .المجموعة الفرعیة الثانیة : تم ري غرفة اللب بلطف مع 10 مل من محلول الھایبوكلوراید 5.25 ٪ لمدة 10 دقائق .المجموعة الفرعیة الثالثة :تم ري غرفة اللب ب 10 مل من محلول الاثیلین دایمین تیترا استك اسد لمدة 10 دقائقالمجموعة الفرعیة الرابعة : تم ري غرفة اللب ب 10 مل من محلول الملحي المتعادل لمدة 10 دقائق2 ملم - تركت جمیع الأسنان لتجف لمدة 24 ساعة ثم ملئت الاسنان بالحشوة المؤقتة. كل سن تم تغطیتھ بواسطة طبقتین من مادة طلاء الاظافر تلیھا طبقة من الشمع اللاصق باستثناء 1حول موقع انثقاب مفترق الجذور .وضع كل سن في قارورة زجاجیة تحتوي على 3 مل من صبغة المیثیلین الزرقاء مخزنة في ( 37 درجة مئویة، ودرجة الحموضة 7 ) و حفظ في حاضنة لمدة 72 ساعة وبعد تطبیقالصبغة، تم غسل الأسنان في المیاه الجاریة لمدة 5 دقائق . تم قطع كل رحى طولیا على طول المحور الطولي في الاتجاه الدھلیزي اللساني من خلال الانثقاب باستخدام القرصالماسي . اظھرت النتائج ان المجموعة الاولى المرممة بمادة البایودنتین كان لھا القیمة الوسطیة النسبیة الاوطا لاختراق الصبغة و كان الاختلاف ذو فرق معنوي احصائي عال جدا معالمجموعة الثالثة المرممة بواسطة مادة الاملكم و كان الفرق بین المجموعة الاولى و الثانیة المرممة بمادة تجمع المعدن ثلاثي الاوكسد غیر معنوي .كما اظھرت النتائج ان المحلول الملحي العادي و و محلول الھایبوكلورایت یزید قابلیة الختم جمیع المجموعات في حین محلول محلول الاثیلین دایمین تیترا استل اسد سبب في زیادةكبیرة في اختراق الصبغة من البایودنتین و مادة و تجمع المعدن ثلاثي الاوكسد على التوالي.

Keywords

Biodentine --- MTA --- EDTA --- NaOCl --- root perforations


Article
Smear Layer Removal and Apical Extrusion Evaluation Among Different Irrigating Techniques and Solutions (In vitro Comparative study)

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Abstract

Objectives: The aim of the present study was to compare the effectiveness ofEndovac, Vibringe and needle with different irrigation solutions on removal ofsmear layer and apical extrusion of irrigating solution.Methods: Seventy-two sound human premolar teeth were used and divided into threeexperimental groups (n=24) according to the type of irrigation technique used: Ingroup1 irrigation was performed with Vibringe. In-group 2 irrigation wasperformed with a 30-gauge side-vented irrigation needle. In-group 3 irrigation wasperformed with Endovac. Instrumentation was performed by using the ProTaperfiles. Each main group divided into three sub-groups (n=8) according to irrigationsolution used: In sub-group 1 Irrigation was performed with 5.25% Sodiumhypochlorite (NaOCL). In sub-group 2 Irrigation was performed with 17%Ethylene Diamin Tetra Acidic Acid (EDTA). In sub-group 3 Irrigation wasperformed with 5.25% NaOCL and 17 % EDTA. The amount of extrudedirrigating solution was then measured by subtracting the weight before finalirrigation from the weight after final irrigation using the electronic balance. Thecleanliness of smear layer removal was evaluated using scanning electronmicroscopy.Results: The results showed that the group that resulted in more irrigation extrusionwas as follow from highest to lowest: side-vented >Vibringe> Endovac. Thedifference among all groups was significant. As for cleaning results, smear layercollection in both EndoVac and Vibringe groups were less than side-vented groupand these differences were significant. When the three irrigating solutionscompared the differences among all the groups were significant and combinationof (5.25% NaOCL and 17 % EDTA) resulted in more smear layer removal.Conclusion: In conclusion, the EndoVac irrigation system extruded significantly lessirrigant solution than both the Vibringe and needle irrigation systems. Smear layercollection was least in the apical third regarding the EndoVac irrigation systemand resulted in more Smear layer removal. And combination of NaOCL andEDTA group was more effective on smear layer removal than other two groups.


Article
Prevalence of Pseudomonas aeruginosa producing Metallo-β- lactamases in wounds and burns infections

Authors: Sabah Hasan Rhadi --- Intidhaar N.Abid
Journal: JOURNAL OF THI-QAR SCIENCE مجلة علوم ذي قار ISSN: 19918690 Year: 2017 Volume: 6 Issue: 3 Pages: 58-67
Publisher: Thi-Qar University جامعة ذي قار

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Abstract

Metallo β -lactamases (MBLs) producing Pseudomonas aeruginosa have been detected from clinical isolates in worldwide with increase in emergence in the last years . The spread of MBLs positive isolates in a localized hospital environment causes not only a therapeutic problem but as well as a serious concern for infection control handling , hence , this study was aimed to determine the prevalence of P. aeruginosa producing MBLs isolated from some skin infections (wounds and burns). A total of 57 P. aeruginosa were isolated from wounds and burns infections (24 wound swabs and 33 burn swabs) in Al-Hussain Teaching Hospital .Primary screening of carbapenems resistant isolates appeared that 63% (36 isolates) were resistant to imipenem and meropenem .Among the 36 carbapenems resistant isolates that were tested for production MBLs by phenotypic test (CDT)with EDTA inhibitor (as chelating factor ), 34 (94%) were MBLs positive and 59.6% from 57 isolates were positive to MBLs production . MICs values of MBLs producers were higher in imipenem ((≥16-64) μg/ml)) than that in meropenem (( ≥16-32) μg/ml)) . Out of 34 MBLs producer , 24 (72.7%) isolates were founded in specimens of burn swabs , while the wound swabs specimens registered 41.6% . MBLs producing isolates were also tested for antibiotics susceptibility , all isolates (100%)were sensitive to aztreonam and 70.5% to ciprofloxacin . 100% of isolates were resistant to cefoxitin and amoxycillin – clavulanic acid.


Article
Physical Properties of Gypseous Soil after Gypsum Removal using EDTA Solution

Author: Farouk Majeed Muhauwiss
Journal: Tikrit Journal of Engineering Sciences مجلة تكريت للعلوم الهندسية ISSN: 1813162X 23127589 Year: 2018 Volume: 25 Issue: 2 Pages: 68-73
Publisher: Tikrit University جامعة تكريت

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Abstract

The main structural problem in construction on gypseous soils is due to the melting of the gypsum when exposed to water. This may be creating voids in the soil leading to rearrangement of the soil structure and moving the soil particles to more stable positions. This can cause excessive settlement which directly affects superstructures. This study, investigates the influence of gypsum removing on granular soil classification. Four gypsum soil specimens were taken from Al-Qadisiyah district in Tikrit at different depths from the natural ground surface. The depths adopted were 0.75, 1.10, 2.00 and 3.30 m. The corresponding gypsum content was 42.23%, 32.50%, 8.75% and 19.82%, respectively. The EDTA solution was used to disassemble and remove the gypsum particles by washing using distilled water. The results showed that EDTA solution and washing with distilled water was an effective method to remove gypsum from granular soils. Gypsum ratio was reduced to less than 2% in all tested specimens. The percentage of organic matter was not affected, and the specific gravity of the specimens increased between 2% and 12%. The gypsum removal process affected the granular distribution curves of the soil specimens and led to a decrease in the rate of soil grain diameters. In general, classification process of the soil before and after the washing of gypsum from the soil was not affected.


Article
Effect of sodium hypochlorite, chlorhexidin and EDTA on dentin microhardness
تأثير هيبوكلوريت الصوديوم ، الكلورهيكسيدين و EDTA على متانة العاج

Authors: Basam Kareem Amin --- Sazan Sherdil Saleem --- Diyar Khalid Bakr
Journal: Zanco Journal of Medical Sciences مجلة زانكو للعلوم الطبية ISSN: 19955588/19955596 Year: 2016 Volume: 20 Issue: 1 Pages: 1175-1179
Publisher: Hawler Medical Univeristy جامعة هولير الطبية

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Abstract

Background and objective: The use of chemical irrigants solution in root canal therapy is capable of altering the proportion of organic and inorganic components of root canals. The structural properties of dentin may be changed such as permeability, solubility and microhardness. The aim of this study was to evaluate the effects of root canal irrigants on the microhardness of root canal by using three types of irrigant solutions with different concentration and normal saline used as control group. Methods: Root halves were prepared by longitudinal splitting of the roots of 56 freshly extracted caries free maxillary second premolars and embedded in autopolymerizing acrylic resin, leaving the dentin surface exposed. The root halves were randomly divided to seven groups composed of 16 samples each and treated for five minutes with one of the following irrigants: normal saline (control group), 0.2 % chlorhexidin, 2% chlorhexidin, 2.5% sodium hypochlorite, 5.25%, 5% Ethylene dimetha tetra hydrate EDTA and 17% EDTA. After surface treatment, the dentin microhardness of the root samples were recorded at the mid-root level by using a vicker microhardness tester. The data were statistically analyzed by using one-way analysis of variance, followed by Duncans test with a significant difference test at P ≤0.05. Results: EDTA, sodium hypochlorite, and 2% chlorhexidin significantly decreased the microhardness of root dentin compared with controls (P <0.05), while 0.2% chlorhexidin had no significant effect on the microhardness of root dentin. Conclusion: The irrigant solutions affect the microhardness of the samples except 0.2%.

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