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An Epidemiological and Diagnostic Study of Toxoplasma gondii by Serological and Molecular Methods Using nPCR Technique in Pregnant and Abortive Women in Mosul and its Environ
دراسة وبائية وتشخيصية لطفيلي المقوسات الكونيدية Toxoplasma gondii بالطرق المصلية و الجزيئية بتقنية nPCR في النساء الحوامل والمجهضات في مدينة الموصل وضواحيهادراسة وبائية وتشخيصية لطفيلي المقوسات الكونيدية Toxoplasma gondii بالطرق المصلية و الجزيئية بتقنية nPCR في النساء الحوامل والمجهضات في مدينة الموصل وضواحيها

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Abstract

The study examined 120 samples of blood and serum of pregnant women who miscarriaged in Al Salam Educational Hospital to investigate their incidence of Toxoplasma gondii since 1/5/2013 to 1/5/2014, using latex test and The Enzyme -Linked Immunosorbent Assay and the Enzyme-Linked Fluorescence Assay, in addition to Toxoplasma gondii (B1) gene by the Nested polymerase chain reaction technique. The results of the study showed that the best technique for acute T.gondii was nested PCR with 36.6% followed by ELISA 31.6%, ELFA 26.6% and Latex 18.3%. ELISA and ELFA tested high sensitivity to anti-IgM (85% and 73%, respectively), based on the results of the nPCR test as a positive control group, Where as latex test revealed a sensitivity of 50%. The acute infection in Mosul and its suburbs was 36.6%. The results showed no significant differences in different age groups, the housing area has an impact on the incidence rates in the suburbs and villages of Mosul, 42%, while in the city 32.8 %, infection rates rise, with the lower educational level of women. The friction of women with animals and cats raise the infection rate to 53.8%, while the percentage dropped to 23.5% in women who did not treat or contact with animals and cats.

تضمنت الدراسة فحص 120 عينة من دماء ومصل النساء الحوامل والمجهضات المراجعات إلى مستشفى السلام التعليمي للتحري عن إصابتهن بداء المقوسات الكونيدية Toxoplasma gondii وذالك للمدة بين 1/5/2013 إلى غاية 1/5/2014، باستخدام اختبار التلازن Latex واختبار الامتزاز المناعي المرتبط بالإنزيمThe Enzyme-Linked Immunosorbent Assay واختبار التألق المرتبط بإنزيم The Enzyme-Linked Fluorescent Assay بالإضافة إلى اختبار التحري عن مجين طفيلي المقوسات الكوندية T.gondii (B1) بتقنية تفاعل البولمر المتسلسل Nested polymerase chain reaction. أوضحت نتائج الدراسة ان أفضل تقنية للتحري عن الإصابة الحادة بداء المقوسات كانت بتقنية تفاعل البوليمر المتسلسل (nested PCR) وبنسبة 36.6% تلتها تقنية ELISA 31.6% ، ELFA 26.6% وLatex 18.3%. أعطى كل من اختباري ELISA و ELFA نسب حساسية عالية للكشف عن ضد (IgM) بنسبة 85% ، 73% على التوالي بالاعتماد على نتائج اختبار الـ nPCR كمجموعة سيطرة موجبة وهذا ما أفتقر له اختبار الـ Latex بحساسية 50%، إذ لوحظت الاصابة الحادة في مدينة الموصل وضواحيها بنسبة 36.6 %، ولم تظهر النتائج فروقاً معنوية لانتشار طفيلي المقوسات الكوندية في الفئات العمرية المختلفة، أن لمنطقة السكن تأثيرا على نسب الإصابة في ضواحي وقرى الموصل 42% بينما في المدينة 32.8%، تبدأ نسب الإصابة بالارتفاع كلما أنخفض المستوى التعليمي في النساء، أن احتكاك النساء بالحيوانات و القطط رفع نسبة الإصابة إلى 53.8% بينما انخفضت النسبة إلى 23.5% في النساء اللاتي لم يكن لهن تعامل او اتصال بالحيوانات والقطط.


Article
ELFA and IFAT Techniques to Detect Chlamydial Infections in Baghdad Women and Its Effect on the Immunoglobulins Level.

Authors: Khamael M. Kadaem* MSc --- Raad K. Al-Husseinei --- Lamya A. AL-Janabi --- Mun'im M. Fathei
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2009 Volume: 51 Issue: 2 Pages: 192-197
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Chlamydia trachomatis is one of the most common human pathogens and considered asone of the causative agents of STDs. This organism cause acute and recurrent pelvic infections andinfertility.Patients and Methods: Two hundred and seventy three females were included in the present study,attending infertility department, AL-Elwiya hospital, AL-Jarah private hospital, central public healthlaboratory and STDs clinic to whom IFAT, ELAF and immunoglobulins concentration were done.Results: Females were divided into three age groups <20; 20-39 and ≥40 years. Single and repeatedabortions were 44.9%, 55.1% respectively. Primary and secondary infertility were 55.6% and 44.4%.Higher abortions rate were in the age group 20-39 years which represents 31.5% and 41.6% in singleand repeated abortions. Primary and secondary infertility were high in the same age group whichrepresents 42.6% and 33.3% of the total investigated females, also the multipartners within the sameage group constitutes 56.7%.Chlamydial infections detected by IFAT technique constitute 12.8% whichwere represented as 14.6%, 11.1%, 36.7% and 5% in abortions, infertility, multipartners and fertilitywith no abortion groups respectively. While by ELFA technique the percentages were 12.4%, 9.3%,30% and 4% in the same mentioned groups.Immunoglobulins mean value in females with chlamydialinfections wee as follows IgG = 2102.1 mg/dl; IgA = 317.9 mg/dl and IgM = 272.5 mg/dl. Which weremore than the normal values of the immunoglobulins.Conclusions: Chlamydial infections were distributed largely among multipartners than other abortedfemales. High abortions were noted in age group 20-39 years. IFAT technique was more reliable thanELFA technique to detect chlamydial infections.IgG and IgM concentrations were higher than normal concentrations, while IgA remains normal.

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