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Article
SEROEPIDEMIOLOGICAL STUDY OF OVINE Q FEVER IN BASRA PROVINCE ,IRAQ
. دراسة وبائية مصلية للتحري عن حمى كيو في الضأن في محافظة البصرة,العراق

Author: Mohanad Hasan Lafta , Rahman Kadhum Muhsen
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: 18138497 Year: 2016 Volume: 15 Issue: 1 Pages: 241-248
Publisher: Basrah University جامعة البصرة

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Abstract

Indirect ELISA test was used to detect specific antibodies against Coxiella burnetti in sheep in different regions of Basra province.184 local sheep breeds were randomly selected of different ages and of both sexes were used in this study, including 98 pregnant ewes, 55 non-pregnant ewes, 8 aborted ewes ,7 ewes of still births, moreover 112 lower than 2 years,more than 2 years 72and 13 rams. Results showed, that the percentage of infection detected by indirect ELISA were 41.84%. It have been concluded that C.burnetti were infected ewes of different status in Basra province, therefor periodic investigation of suspected animals must adviced. were a significant between, areas, age, groups pregnancy status and still birth ewes .incontrast non significant different were detected among gender , abortion .

Keywords

ELISA test --- ELISA test --- Sheep


Article
PREVALENCE OF Q- FEVER IN SMALL RUMINANTS IN AL-QASSIM CITY
انتشارحمى كيو في المجترات الصغيرة في مدينة القاسم

Author: Qassim Haleem Kshash قاسم حليم كشاش
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: 18138497 Year: 2012 Volume: 11 Issue: 1 Pages: 342-348
Publisher: Basrah University جامعة البصرة

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Abstract

The present study was carried out on 500 of small ruminants ( sheep and goats ) to determine the prevalence of Coxielosis in small ruminants in AL-Qassim city by using ELISA test .The results showed 16% of small ruminants in AL-Qassim city were seropositive to coxiellosis . Infection in sheep ( 19.4% ) was higher than in goat (8% ) .The persent studywas concluded that coxiellosis did not affected by sex and age of examined animals.


Article
Purification of Exotoxin A from Locally Isolate of
تنقية السم الخارجي من نوع A المنتج من قبل العزلة المحلية لبكتريا

Author: Sara K. Nafea, Subhi J. Hamza, Maha F. Majeed
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2013 Volume: 12 Issue: 1 Pages: 68-76
Publisher: Baghdad University جامعة بغداد

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Abstract

Twenty-five isolates of Pseudomonas aeruginosa were isolated from wounds, burns, inflammation of the urinary tract(UTI), sputum and ear infections. These isolates were found producing exotoxin A as indicated by ELISA test. Isolates from different infections (burn, wounds and UTI) were selected according to their productivity. An amount of 0.045, 0.064 and 0.040 mg/ml protein which represent and 23.31, 29.73 and 19.01 ng/ml exotoxin A were produced respectively. Extoxoxin A was partially purified using precipitation by 90% ammonium sulphate followed by ion exchange chromatography with DEAE-cellulose then gel filtration chromatography by sepharose 4B was using to detection the molecular weight for the purified toxin was estimated as 65 KD.

تم إختبار 25 عزلة من بكتريا Pseudomonas aeruginosaالمعزولة محلياً من الجروح, الحروق, التهابات المسالك البولية, القشع والتهابات الأذن. تم الكشف عن قابلية هذه العزلات على إنتاج السم الخارجي من نوع A بأستعمال نظام ELISA, وقد تم إختيار هذه العزلات من الجروح, الحروق والتهابات المسالك البولية وفقا لأنتاجيتها إذ بلغت 0.045, 0.064 و 0.040 ملغم/مل للبروتين و 29.73, 23.31 و19.01 نانوغرام/مل للسم الخارجي نوع A على التوالي. تمت التنقية للسم الخارجي بواسطة الترسيب بكبريتات الامونيوم بنسبة إشباع90% تليها التنقية بالمبادل ألايونيDEAE-cellulose , والتنقية بالهلام من نوع Sepharose 4B وتعيين الوزن الجزيئي للسم الخارجي الذي قدر بـ 65 كيلودالتون.


Article
Serological Study Of Toxocariasis In Salah Al-Din Province
دراسة مصلية لداء السهميات Toxocariasis في محافظة صلاح الدين

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Abstract

The present study included the examination of 372 blood samples from outdoor patient to private general hospitals and civil labratory in Salahuddin province. Both sexes ranged in age from 1-61 years during the period from 1 st July. 2012 to 1 st July. 2013 were examined for infection by toxocariasis using ELISA (IgG TES - ELISA). The results showed that the percentage of ELISA positive serum samples was 6.98 % and the highest number of laboratory positive results was in the age group 11-20 years (9.72%). The study showed significant differences in the relationship between age , sex , pets dogs , educational level , and location has recorded the highest percentages was recorded in the age group of 11-20 years by using T-tsest & Duncun Multiple Range in the significance level of P > 0.05 , and the results were as follows : the proportion of infection male 6.58 % less than females 8.37 % , acquisitions 6.12 % of dogs and not peting dogs 7.55 % , the highest rate of infection of 12.82 % in the non-educated , the highest infection rate of 13.55 % , 10.63 % , 9.67 % recorded in the rural areas in Baiji and saniah hand and Amerli spend in Douz , respectively .

شملت الدراسة فحص 372 عينة دم من المراجعين للمستشفيات العامة والمختبرات الأهلية في محافظة صلاح الدين من كلا الجنسين تفاوتت أعمارهم بين 1- 61 سنة خلال المدة من 2012/7/1 إلى 2013/7/1 للتحري عن الخمج بداء السهميات Toxocariasis باستعمال اختبار الاليزا (ELISA IgG TEST-) . وبينت نتائج فحص الاليزا إن عدد عينات المصل الموجبة كانت 6.98% وأعلى عددا من الخمجين كان ضمن الفئة العمرية 11-20 سنة . و بينت الدراسة وجود فروق معنوية في العلاقة بين العمر ، الجنس، اقتناء الكلاب، المستوى التعليمي، وموقع السكن وقد سجلت أعلى النسب ضمن الفئة العمرية من 11-20 سنة (9.72%) ، وكانت النتائج كما يلي: كانت نسبة خمج الذكور 6.58% مقارنة مع الإناث 8.37% ،اقتناء الكلاب 6.12% مقارنة بعدم اقتناء الكلاب 7.55% ، أعلى نسبة خمج 12.82% في غير المتعلمين ، أعلى نسبة خمج 13.55 %، 10.63 %، 9.67 % سجلت في المناطق الريفية في قضاء بيجي و ناحية الصينية وناحية آمرلي في قضاء الطوز ، على التوالي .


Article
Detection viral load of Parvovirus B19 in patients with Chronic Renal Failure

Authors: Sattar G. Ajeel --- Huda D. hathal --- Jawad K. mnate
Journal: Mustansiriya Medical Journal مجلة المستنصرية الطبية ISSN: 20701128 22274081 Year: 2016 Volume: 15 Issue: 3 Pages: 40-44
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Background : Because of Parvovirus B19 that occur in patients with chronic renal failure ; Parvovirus B19 was found to be associated with or might be an aggravation factor of renal failure , we tried to find out the rate of occurrence of this virus in dialysis and non dialysis patients. Objective: The aim of the study was to investigate viral load for Parvovirus B19 was play an important role in the etiology of chronic renal failure patients Subjects and methods :A cross sectional study included Serum samples were collected from 50 dialysis patients and 50 without dialysis patients 50 normal subjects as control, and subjected for ELISA technique for detection of parvovirus IgG antibody, and multiplex Real time PCR for detection virus and viral load of this virus Results: Thirteen (26%) out of these 50 dialysis patients were positive by RT-PCR and, 16% (8/50) were positive by ELISA, while none of the controls was positive neither by RT-PCR nor by ELISA. The results of this study showed highly significant differences (p<0.001) on comparing between the median viral load in patients who had dialysis (2.38x106) copies / ml of Parvovirus B19 DNA and those who without dialysis(1.73x104) copies/ml of Parvovirus B19 DNA. Also the results of B19 by real time PCR analysis showed a significant difference (p<0.001) in median viral load in patients who had acute infection(2.87x106) copies/ml of Parvovirus B19 DNA ,than those who had chronic infection (2.29x105) copies/ml of Parvovirus B19 DNA , Conclusions Parvovirus B19 could be an important co-factor that play a role in CKD, which was higher rate in dialysis patients.


Article
Prevalence of Toxoplasma gondii among immunocompromised patients (heamodialysis and cancer) in the province of Thi-Qar-Iraq

Authors: Bassad A.AL-Aboody --- Noor Kadhim Matar AL-Rekaby
Journal: JOURNAL OF THI-QAR SCIENCE مجلة علوم ذي قار ISSN: 19918690 Year: 2017 Volume: 6 Issue: 3 Pages: 101-106
Publisher: Thi-Qar University جامعة ذي قار

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Abstract

The current study was conducted in Thi-Qar province to investigate of Toxoplasma gondii prevalence among renal dialysis and cancer patients by using anti-Toxoplasmosis IgG and IgM by ELISA also the study determined of some factors that may affected the prevalence of infection, such as age, sex, marital status, place of residence and a number of chemical dosing that exposed to it cancer patient or the number of times the dialysis for renal dialysis patients. The results of the current study showed that the prevalence of toxoplasmosis in Thi-Qar province in both renal dialysis and cancer patients and control group were 28% and 36% and 24% respectively. recorded results of Enzyme Linked Immunosorbent Assay ELISA test results for a positive antibody IgG in both renal dialysis and cancer patients and control group 25% , 30% , 22% respectively. As for the IgM antibody study did not record any positive result in both categories of the study (dialysis and cancer) only control group was 2%. The result of IgG & IgM together in renal dialysis and cancer and control group 3%, 6%, 0% respectively.The study found that the increase in the number of times the dialysis increased incidence of infection prevalence it was 25.53% for patients who conducted the dialysis treatment more than six times and 16.66% for patients who have less than six times.For factor chemical dosing show high infection rates in patients who have been exposed to more than one dose of a chemical 37.63% As for those who were not exposed to any chemical dose was ratios have 14.29% .


Article
The Role of Elisa test in the diagnosis of helicobacter pylori infection
دور اختبار إليزا في تشخيص إصابة هيليكوباكتر بيلوري

Authors: Nassir E. Mohsun ناصر نصيف محسن --- Raji H. AL-Hadithi --- Suhaila saadallah
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2011 Volume: 53 Issue: 3 Pages: 311-313
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Helicobacter pylori represents the major etiologic agent of gastritis, gastric and duodenal ulcer disease and can cause gastric cancer. Diagnostic testing for Helicobacter pylori can be divided into invasive and non-invasive techniques based upon the need for endoscopy. Serological test is one of the non – invasive tests although measuring these antibodies is not reliable method of diagnosis but may be used in certain condition.Objectives: To evaluate serum IgG antibodies against Helicobacter pylori by ELISA technique.Patients and Methods: The current study consisted of 115 patients (74 males, 41 females) attending The Gastrointestinal tract Center and Gastroscopy department in Baghdad Medical City and was subjected to gastroscopy, during the period from November 2004 to May 2005. Those how were examined for serum IgG against Helicobacter pylori by ELISA technique were compared with 10 apparently healthy individuals representing the control group. Results: By using the serological method (ELISA) 85 patients out of the 115 showed positive results (73.91%), however 7 out of the 10 individuals representing the control group were serologically positive (70%).Conclusion: Positive IgG antibody test for Helicobacter pylori indicates a marker for infection rather than an indicator for active infection.

خلفية: هيليكوباكتر بيلوري يمثل عامل سببي الرئيسي لالتهاب المعدة، والمعدة والإثنى عشر مرض القرحة، ويمكن أن يسبب سرطان الجهاز الهضمي. ويمكن تقسيم الاختبارات التشخيصية للهيليكوباكتر بيلوري إلى التقنيات الغازية وغير الغازية على أساس الحاجة إلى التنظير. اختبار الأمصال هي واحدة من غير - الاختبارات الغازية على الرغم من أن قياس هذه الأجسام المضادة ليست طريقة يمكن الاعتماد عليها في التشخيص ولكن يمكن استخدامها في حالة معينة. الأهداف: لتقييم مصل الأجسام المضادة ضد هيليكوباكتر بيلوري بواسطة تقنية ELISA. المرضى والطرق: وتتألف الدراسة الحالية من 115 مريض (74 ذكور، 41 إناث) الذين حضروا الجهاز الهضمي والمسالك مركز قسم الجهاز الهضمي في المدينة الطبية في بغداد وتعرض لتنظير المعدة، خلال الفترة من نوفمبر 2004 الى مايو 2005. وتمت مقارنة هؤلاء كيف تم فحص لمصل ضد مفتش الملوية البوابية بواسطة تقنية ELISA مع 10 أشخاص اصحاء تمثل السيطرة على المجموعة. وأظهر من خلال استخدام أسلوب المصلية (ELISA) 85 مريضا من أصل 115 نتائج إيجابية (73.91٪)، ولكن 7 من الأفراد الذين يمثلون 10 مجموعة المراقبة كانت إيجابية مصليا (70٪): نتائج. والخلاصة: الإيجابية لاختبار الأجسام المضادة مفتش هيليكوباكتر بيلوري يدل على علامة للإصابة وليس مؤشرا للإصابة نشطة


Article
Clinical and Diagnostic Study of Foot and Mouth Disease by using ELISA test in Cattle of AL – Diwaniya
دراسة سريرية و تشخيصية لمرض الحمى القلاعية باستخدام فحص الاليزا في أبقار مدينة الديوانية

Authors: Y. A. Ajeel يسرى عواد عاجل --- M. A.N. Alordhan محسن عبد نعمة --- Q. H.Kshash قاسم حليم كشاش
Journal: Al-Qadisiyah Journal of Veterinary Medicine Sciences مجلة القادسية لعلوم الطب البيطري ISSN: 18185746 23134429 Year: 2012 Volume: 11 Issue: 2 Pages: 91-98
Publisher: Al-Qadisiyah University جامعة القادسية

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Abstract

The study was carried during the period from November 2010 to June 2011 in different areas of AL – Diwaniya city (AL–Sedeer , Afak , AL – Hamza , AL – Shamiya and AL – Bedeer ), clinical examination of animals , blood samples has been collected .The results of clinical study showed the typical and characteristic signs which were differed in the severity as high fever , severe congestion of mucous membrane , profound depression , profuse salivation, eruption of vesicles and stomatitis while lameness , smacking , vesicles on feet were less in the severity ,other clinical signs as mastitis ,sloughing of the hoof and mortality in young animals were not recorded . The infection percentage in different areas of AL– Diwaniya city were 39.4%,33.3%, 26.6% ,20% and 14.2% in (AL–Sedeer , afak, AL– Hamza ,AL–Shamiya and AL– Bedeer) respectively.The infection distribution percentage by ELISA test in clinically affected and apparently normal cows were 93.3 %, 3.44% respectively with a total percentage of 34.09 % .The results showed high sensitivity and specificity of ELISA (93.3 % ,96.5 %) respectively as compare with clinical study.

أجريت الدراسة للفترة من 1 تشرين الثاني 2010 ولغاية 1 حزيران2011 في مناطق مختلفة من مدينة الديوانية والتي شملت منطقـة السدير، عفك ، الحمزة ، الشامية و آل بدير إذ اجري الفحص السريري لقطعان الأبقار وجمعت منها عينات الـدم. وأظهرت نتائج الفحص السريري ظهور العلامات السريرية النموذجية المميزة للمرض والتي تفاوتت في حدوثها بين الشديدة والمتوسطة والبسيطة، فقد كانت الحمى واحتقان الأغشية المخاطية والاكتئاب و زيادة اللعاب و ظهور الحويصلات في الفم تتميز بالشدة في حـين كان ظهور العلامات الأخرى كالحويصلات في القـدم والعـرج و تلمض الشـفاه بين المتوسطة والبسـيطة ولم يسجل ظهـور بعض العـلامات ومنها التهـاب الضرع وانسلاخ الظـلف و الهلاكات في العجول. كانت النسبة الكلية للإصابة السريرية قد توزعت في المناطق المختلفة التي شملتها الدراسة وهي السدير،عفك ،الحمزة ،الشامية وال بدير وبنسبة إصابة 39.4%،%33.3،%26.6،%20 و%14.2 على التـوالي ، كما أظهرت نتائـج فحص الممتز المـناعي المرتبط بالإنزيم غير المباشر في الأبقار التي أظهرت العلامات السريرية والأبقـار التي لم تـظهر عليها العلامات السـريرية النموذجية للإصابة نسب انتشار للمرض 93.3% و% 3.44 على التوالي وبنسبة كلية بلغـت % 34.09. بينت الدراسة الحـالية تطـابق نتائـج فحص الالـيزا مع نتـائج الفحص السريري مسجلة حساسية وخصوصية عاليتين نسبيا %93.3 و%96.5 على التوالي.


Article
Some molecular and sero – prevalence study of Anaplasma marginale in cattle in Wassit province
دراسة مصلية و جزيئية عن مرض الانابلازموسس في الابقار في محافظة واسط

Authors: Ola Abd-El Hussain Agaar علا عبد الحسين عكار --- Ghyda'a Abbas Jassem غيداء عباس جاسم
Journal: Al-Qadisiyah Journal of Veterinary Medicine Sciences مجلة القادسية لعلوم الطب البيطري ISSN: 18185746 23134429 Year: 2015 Volume: 14 Issue: 1 Pages: 27-33
Publisher: Al-Qadisiyah University جامعة القادسية

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Abstract

The study was designed to investigate the prevalence of bovine anaplasmosis among cattle from various areas in Wassit governorate. The investigation was performed on 184 blood samples collected from suspected cattle including (85) males and (99) females, aged from < 1year to > 2 years. Samples were collected during the period from October 2012 to April 2013, from AL-Kut, AL-hayy, AL-Bashair, AL-Moufaqia and AL-Noamania areas for investigations about the presence of antibodies against Anaplasma parasite by using indirect ELISA test and to determinate the species of genus Anaplasma by using RFLP-PCR technique. The suspected cattle suffering from fever (41°C), severe anemia, pale mucus membrane, progressive emaciation and drop in milk yield. The results of ELISA test showed that the rate of infection was 13.04 %; the rate of infection was different between age groups and was 8 %, 11.25 % and 16.45 % in ages < 1year, 1-2 year and 2 - 3 years respectively. The study revealed that females were given higher percent of infection 14.14 % than males 11.7 %, and there were no significant differences under p > 0.05 according to age groups and sex. The highest rate of infection was recorded in AL-Kut 17.14 % followed by AL-hayy 14 % and AL-Bashair 10 % and the lowest rate was recorded in AL-Noamania 8.33 % and AL-Moufaqia 5%. The study showed significant differences in incidence of disease between the studied districts and areas in Wassit governorate under p < 0.05. The most sensitive method for the diagnosis of Anaplasmosis was the method of polymerase chain reaction. The DNA extraction was performed only on 24 blood samples which positive for Anaplasma spp. by ELISA test. The extracted DNA from blood cells were analyzed by PCR and PCR-RFLP technique using primers derived from 16S rRNA gene and restriction endonuclease Bst1107I enzyme which can recognizes the sequence (GTATAC) in corresponding PCR product of A. marginale and cut it in the position 68 and 509, whereas the used restriction enzyme cannot cut the corresponding PCR product of other Aanaplasma spp., and the result was 20 out of 24 was positive for Aanaplasma spp. by PCR and 18 out of 20 was positive for A. marginale.

صممت الدراسة للبحث في انتشار مرض الانابلازموسسس بين قطعان الابقار في مناطق مختلفة من محافظة واسط وقد اجري البحث على 184 عينة دم جمعت من ابقار مشكوك في اصابتها وشملت (85) ذكر و (99) انثى تتراوح اعمارها بين اقل من سنة الى اكثر من سنتين , جمعت العينات خلال الفترة من تشرين الثاني 2012 الى نيسان 2013 من مناطق الكوت , الحي , البشائر, الموفقية و النعمانية للبحث عن الجسام المضادة الخاصة لطفيلي الانابلازما بواسطة طريقة الاليزا الغير مباشر ولتحديد النوع الخاص بجنس الانابلازما بواسطة تقنية تفاعل سلسلة البلمرة (PCR) وتفاعل سلسلة البلمرة نوع تقييد طول الجزء متعدد الأشكال (RFLP-PCR). الابقار المشكوك بها تعاني من الحمى (41م° ) , فقر دم شديد , شحوب الاغشية المخاطية , هزال شديد وانخفاض في انتاج الحليب. أظهرت نتائج فحص الاليزا ان نسبة الاصابة (13.04%) , وكانت نسبة الاصابة مختلفة بين مجاميع الاعمار فكانت بنسبة 8)%) , ((%11.25 و(16.45%) بعمر أقل من سنة , 1 - 2 سنة ومن 2 – 3 سنة على التوالي. أظهرت الدراسة نسبة الاصابة في الاناث ( %14.14) أعلى منها في الذكور11.7) %) , الا انه لا يوجد فرق معنوي تحت مستوى احتمال 5%. اعلى نسبة اصابة سجلت في الكوت17.14 % تتبعها الحي %14 و البشائر10% أما اقل نسبة اصابة سجلت في النعمانية% 8.33 و الموفقية 5% مع فرق معنوي تحت مستوى احتمال 5% . بينت النتائج ان الطريقة الأكثر حساسية في تشخيص الانابلازموسس هي تفاعل سلسلة البلمرة (PCR) حيث تم استخلاص الحمض النووي DNA من 24عينة فقط والتي كانت موجبة لفحص الاليزا وقد تم تحليل الحمض النووي المستخرج بواسطة تقنية PCR و (RFLP_PCR ) باستخدام بادئات مستمدة من 16S rRNA و انزيم القطع endonuclease Bst1107I الذي يميز تتابع القواعد النتروجينية (GTATAC) لنواتج PCR الخاصة A. marginale ويقوم بقطعها بالموقع 68 و 509 في حين ان الانزيم لا يقطع نواتج PCR الخاصة بالأنواع الاخرى لطفيلي الانابلازما وكانت النتيجة20 من24 عينة موجبة لل Anaplasma spp.و18 من 20 موجبة للنوع A. marginale .


Article
In Iraq, First Documentation of Canine Brucellosis by Application of Three Techniques (Rapid test, Indirect ELISA and 16S rDNA Inter-spacer PCR)

Author: Hams Hussien Hashim Handool Alfattli
Journal: Kufa Journal For Veterinary Medical Sciences مجلة الكوفة للعلوم الطبية البيطرية ISSN: 20779798 Year: 2016 Volume: 7 Issue: 2 Pages: 102-110
Publisher: University of Kufa جامعة الكوفة

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Abstract

Brucella canis is a Gram-negative organism infecting, mainly, the genital organs of both sexes and resulted in several reproductive problems. This bacterium is excreted in urine, milk, fetuses or semen of infected dogs, and transmitted to sensitive dogs through sexual, oral, nasal and conjunctival routes. In general, the routine detection of infection is done by serological tests and the confirmation through isolation of causative agent by the culture. Previously, many global studies documented the excellent efficacy of polymerase chain reaction in detecting the bacterial DNA, perfectly and high accuracy. The present study is the first Iraqi document that dealt with the diagnosis of B. Canis, serologically by Rapid test and Indirect ELISA test, and demonstration the infection, molecularly, in seropositive dogs by “16S rDNA inter-spacer PCR” technique. Serologically, the study revealed that 14 (5.76%) and 31 (12.76%) dogs were positive with Rapid test and Indirect ELISA, respectively, while only 5 (16.13%) dogs were positives molecularly. As well as, the received serologic data exhibited that all positive samples with rapid tests were, also, positives by indirect ELISA. At level of P  0.05, the statistical differences were reported within the applied techniques, positive dogs with most common risk factors (sex and age). In relation to sex, the results appeared that the females had an infection rate more than males; while in association to age, the infection rates were similar in both first groups (<4 and 1-4 years) and increased apparently in the last aged group ( 4 years).

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From To Submit

2017 (1)

2016 (5)

2015 (2)

2013 (2)

2012 (2)

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