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Article
SEROEPIDEMIOLOGICAL STUDY OF OVINE Q FEVER IN BASRA PROVINCE ,IRAQ
. دراسة وبائية مصلية للتحري عن حمى كيو في الضأن في محافظة البصرة,العراق

Author: Mohanad Hasan Lafta , Rahman Kadhum Muhsen
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: 18138497 Year: 2016 Volume: 15 Issue: 1 Pages: 241-248
Publisher: Basrah University جامعة البصرة

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Abstract

Indirect ELISA test was used to detect specific antibodies against Coxiella burnetti in sheep in different regions of Basra province.184 local sheep breeds were randomly selected of different ages and of both sexes were used in this study, including 98 pregnant ewes, 55 non-pregnant ewes, 8 aborted ewes ,7 ewes of still births, moreover 112 lower than 2 years,more than 2 years 72and 13 rams. Results showed, that the percentage of infection detected by indirect ELISA were 41.84%. It have been concluded that C.burnetti were infected ewes of different status in Basra province, therefor periodic investigation of suspected animals must adviced. were a significant between, areas, age, groups pregnancy status and still birth ewes .incontrast non significant different were detected among gender , abortion .

Keywords

ELISA test --- ELISA test --- Sheep


Article
Preparation of Laboratory Kit for Lipoteichoic acid as One of the Constituents of Gram-Positive Bacterial Cell Wall
تحضير عدة الفحص Kit مختبريا لحامض التيكويك الدهني Lipoteichoic acid، احد مكونات جدار الجراثيم الموجبة لصبغة كرام

Authors: Amera M. M. Al-Rawi أميرة محمود محمد الراوي --- Tuka M. Al-Sawaf تُقى مؤيد بكر الصواف
Journal: Rafidain journal of science مجلة علوم الرافدين ISSN: 16089391 Year: 2012 Volume: 23 Issue: 1A Pages: 1-10
Publisher: Mosul University جامعة الموصل

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The study includes the production of antibody for LTA in the rabbit after isolation and purefication from S.aureus and St.viridans by injecting the animals regullary and consecutively with pure LTA. Kit was prepared to investigate the presence of antibodies in sera of injected animals by ELISA, the absorbance of the test samples for each of S.aureus - LTA and St.viridans - LTA were 1.0700 ± 0.0141 and 1.0100 ± 0.0141 respectively, The absorbance of control sample, which represent sera of lab animals injected with distilled water was 0.9300 ± 0.0141.

تضمنت الدراسة إنتاج الأجسام المضادة لـحامض التيكويك الدهني LTA في الأرانب بعد ان تم عزله وتنقيته من جرثومتي S.aureus و St.viridans ، وذلك بحقنها بجرعات منتظمة ومتتالية من معلق LTA النقي بكميات متزايدة تدريجيا من المعلق المحضر وحضرت عدة فحص Kit مختبريا للتحري عن وجود الأجسام المضادة في أمصال الأرانب المحقونة به بتقنية الاليزا ELISA إذ بلغت امتصاصية عينات الاختبار لكل من S.aureus - LTA وSt.viridans – LTA (0.0141 ± 1.0700 و 0.0141 ± 1.0100) على التوالي كما بلغت قيمة امتصاصية عينات السيطرة التي تمثل أمصال الحيوانات المختبرية المحقونة بالماء المقطر (0.0141 ± 0.9300).


Article
COMPARATIVE STUDY OF ENZYME LINKED IMMUNOSORBANT ASSAY AND AGGLUTINATION TESTS IN THE DIAGNOSIS OF HUMAN BRUCELLOSIS IN BAGHDAD

Authors: Haidar A Shamran حيدر احمد شمران --- Haidar F Ghazi حيدر فيصل غازي --- Jabbar S Hassan جبار سلمان حسان
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2011 Volume: 9 Issue: 2 Pages: 170-175
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background:Human infection with Brucella spp. had been able to evoke humeral immune response containing both IgG and IgM.Objective:This study designed to compare results obtained from Rose Bengal Test (RBT), Tube Agglutination Test (TAT) and Enzyme Linked Immunosorbent Assay (ELISA) employ serum are described and compared for the detection of human IgG and IgM anti-brucella antibodies.Methods:Serum samples from 105 subjects were collected. 90 were clinically infected with human brucellosis, and 15 were age and gender matched controls. RBT and TAT are the two screening tests routinely recognized, while the 2-mercaptoethanol test (2ME) is the confirmatory assays currently in use. In order to improve the serological diagnosis of human brucellosis, an indirect IgG, IgM and IgG-IgM ELISA kits were evaluatedResults:Totally, 90 cases were positive in RBT, from those only 92% shows positive TAT, and by ELISA there are IgG (27.78%), IgM (14.44%) and (57.78%) were Positive for both immunoglobulins.Conclusion:Although RBT and TAT are widely applied tests, they cannot differentiate acute and chronic states of brucellosis. Our data suggest that IgM ELISA may be a suitable test for diagnosis acute brucellosis.Keywords: Brucellosis, ELISA, Tube Agglutination.


Article
H. Pylori Infection Among Adults Undergoing Gastrointestinal Endoscopy

Author: Leen K.Mustafa Kamil
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2007 Volume: 6 Issue: 1 Pages: 37-40
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACK GROUND:INTRODUCTION: Helicobacter Pylori was brought to the worlds attention 1983by Warren and Morshall, it is now acknowledged that H.Pylori gastritis is the one of the most common human bacterial infectious disease and is causally linked with gastritis , peptic ulcer disease, gastric adeno- carcinoma ,and gastric B.cell lymphoma.(1)H.Pylori is a slow growing , microaerophilic, highly motile, Gram negative spiral organism whose most striking biochemical characteristic is the abundant production of urease enzyme which is an important indirect marker of the organisms presence because it is the bases of biopsy rapid unease test, the urea broth test and as an antigen for serologic detection . The prevalence of H.Pylori among healthy individuals varies depending on age , socioeconomic class, country of origin. In developing countries children are typically infected by age 10 years, whereas in developed countries there is an age related increase in prevalence (1,2 ).The major risk factor for infection is the socioeconomic status of the family during childhood as reflected by number of persons in a house hold, sharing a bed ,and absence of a fixed hot water supply all of which probably are markers for the level of sanitation and house hold hygiene(3,4,5 ).* Immunology Unit, Teaching Laboratories-Medical CityIt is not known how often an acute infection with H.Pylori sponteneously clears , studies in children suggest that sponteneous loss of infection may be common (6 ).Infection in adults appears to be typically long lived and is probably life long.(7 ) . Most infected individuals have chronic active, non atrophic superficial gastritis .This histological form is usually asymptomatic but may be associated with duodenal ulcer; chronic atrophic gastritis , gastric adeno carcinoma or gastric lymphoma. (6,7 ) Diagnostic tests for H.Pylori can be divided into those that do and do not require samples of gastric mucosa, mucosal biopsy of histological examination of the specimen for the presence of H.Pylori and or gastritis has been the diagnostic method of choice until recently :to increase diagnostic yield ,use of large cup biopsy and 3 samples biopsy (lesser curve Angularis ,greater curve pre pyloric and greater curve body ) examined by both Giemsa stain as a standard stain and hematoxylin & eosin stain which is excellent to determine histologic chronic or chronic active gastritis and demonstrates H.Pylori if large number of organisms are present ( 1,6) . Biopsies may also be tested for the presence of unrease enzyme production by agar gel slide test such as rapid urease test which is excellent for screening for the presence of H.Pylori in patients with peptic ulcer.Tests that do not require a mucosal biopsy include serologic tests as urea broth test, detection ofinfection in adults To determine the prevalence of H.Pylori undergoing oesphagogastrodudenoscopy by two methods serology (ELISA technique) comparing it with histopathology.METHODS:Forty patients referred to the GIT clinic of AL-Yarmok teaching hospital for GI endoscopy were involved in this study; their biopsies and sera send to histopathology and immunology department respectively for detection of H.Pylori. RESULTS:H.Pylori Abs(IgG) were detected in the sera of 25(63%) patients by ELISA,15 (37.50%) of them H.Pylori was also seen in their biopsies by Giemsa s stain. Most patients with detectable antibodies are those with chronic gastritis ;however patients complaining from reflux oesophagitis showed a significant absentees of these Abs.CONCLUSION:Most patients with gastritis had detectable H,Pylori Abs in their sera; However the study reveled a significant decrease in H.Pylori Ab detection in patients sera with reflex esophagitis (R.O).

Keywords

Endoscopy --- H.Pylori --- ELISA


Article
Detection of streptomycin residues in local meat of bovine and ovine
الكشف عن بقايا (ثمالات) الستربتومايسين في اللحوم المحلية للأبقار والضأن

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Abstract

From meat retails in Mosul province, forty-five meat samples of local ovine and bovine (23 bovine samples and 22 ovine samples) were collected. The period of collection was during November 2010 to May 2011, by means of multistage random sampling for detection of streptomycin residues. Enzyme linked immunosorbent assay (ELISA) was used for detection of streptomycin residues. The results revealed that eleven ovine meat samples (50%) were positive to streptomycin residue, with a mean value 35.06 µg kg-1, while 14 bovine meat samples (60.86%) were positive to residual streptomycin with a mean value 59.56 µg kg-1. From the results, it is clear that all tested meat samples (ovine and bovine) were safe enough for human consumption.

تم جمع 45 عينة للحوم الأبقار والأغنام المحلية (23 عينة للحوم الأبقار و22 عينة للحوم الأغنام) من مختلف أسواق اللحوم في مدينة الموصل خلال الفترة الواقعة بين تشرين الثاني 2010 و أيار 2011 وبصورة متعاقبة وعشوائية للكشف عن بقايا المضاد الحيوي ستربتومايسين. استخدمت تقنية الادمصاص المناعي المرتبط بالأنزيم في الكشف عن بقايا المضاد الحيوي (الستربتومايسين). أوضحت النتائج أن 11 عينة للحوم الأغنام (50 %) كانت موجبة لبقايا المضاد وبمعدل 06,35 مايكرو غرام /كغم وان 14 عينة للحوم الأبقار كانت موجبة (86‚60 %) لبقايا المضاد وبمعدل 59,56 مايكروغرام/كغم. من النتائج المتحصل عليها يتضح أن جميع عينات لحوم الأبقار والأغنام صالحة للاستهلاك البشري.

Keywords

Streptomycin --- local meat --- ELISA


Article
BLOOD PROGESTERONE AND ESTROGEN HORMONES LEVEL DURING PREGNANCY AND AFTER BIRTH IN IRAQI SHEEP AND GOAT
قياس مستوى هورموني البروجسترون والاستروجين خلال الحمل وبعد الولادة

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Abstract

Serum progesterone and estrogen concentrations were investigated during pregnancy and few days after birth. Blood samples were collected twice / month from 24 numbered animals ( 12ewes and 12 does) . serum was isolated and kept under -20C untill hormonal analysis. Enzyme - Linked Immunosorbent assay (ELISA) using (ELISA Reader Dona 3200). Progesterone concentrations of pregnant ewes and does were showed steadily increased to reach 24.9+ 2.5 ng/ml and 30.34+ 2.3ng/ml in ewes and does, during 4th month and declined to 0.6 and 2.5 ng/ ml after birth in ewes and does, respectively. Estrogen hormone levels in ewes were increased significantly during pregnancy to maximum 98.7+4.3ng/ml by 5th month and sharply declined to 4.1+0.06 after birth. While in does estrogen level increased significantly and steadily to 1150.6+ 6.23 pg/ml during last month of pregnancy and to 5.9+ 0.4 pg/ml after birth. The present work indicated levels of progesterone hormone increased during 1st and 2nd months in ewes and does, while the significant increased from 3rd month on . Estrogen reach maximum concentrations during last month in doe Which higher about 11 times than that of ewes .It is useful means to diagnosis pregnancy of ewes and does by hormonal methods after mating 20-30 days.

يهدف البحث لقياس مستوى هرموني الحمل البروجسترون والاستروجين وبعد الولادة. جمع الدم من 24 رأس (12 نعجة و12 معزة )مرتين في ألشهر من كل حيوان . فصل وحفظ مصل الدم وتم قياس الهرمونات بطريقة Enzyme –linked immunosorbent assay(ELISA) وباستخدام (ELISA Reader Dona 3200) أعلى مستوى لهرمون البروجسترون كان في الشهر الرابع من الحمل 24.9+2.53 و 30.34+2.23 نانوعرام /مل في الأغنام والمعز وينخفض بصورة حادة بعد الولادة الى 0.6 و 2.5 نانوغرام /مل في الأغنام والماعز على التوالي . أما هرمون الاستروجبن في الأغنام والماعز فكان يزداد بصورة تصاعدية ومعنوية خلال أشهر الحمل ليصل إلى أعلى مستوى له في الأغنام 98.65+ 2.4 والماعز 1150.6+ 6.23 بيكوغرام / مل خلال الشهر الأخير من الحمل . بعد الولادة ينخفض إلى اقل مستوى فيصل 4.1 للأغنام و 5.9 للماعز. نستنتج من هذه الدراسة بان هرمون البروجسترون يرتفع خلال الشهر الثاني والثالث ليصل إلى أعلى مستوى للأغنام والماعز خلال الشهر الرابع للحمل .هرمون الاستروجين في الماعز يزداد خلال الحمل تصاعديا ويصل أعلى مستوى قبل الولادة و يصل مستو الهرمون 11 مرة أكثر مما في النعاج . من المفيد تشخيص الحمل في الأغنام والماعز بقياس هرمونات البروجستون والاستروجين بعد التسفيد أو التلقيح بـ 20-30 يوم. خلال أشهر الحمل

Keywords

: progesterone --- ELISA --- pregnancy


Article
ALLEREGENIC ACTIVITY OF BOVINE DANDER EXTRACT
الفعالية المستارجة لخلاصة جلد الابقار

Author: Sundus S.Baker سندس صديق بكر
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: 18138497 Year: 2006 Volume: 5 Issue: 1 Pages: 112-116
Publisher: Basrah University جامعة البصرة

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This study was conducted in Basrah at period from March through July 2000.The protein extract from Bovine dander was prepared with extraction followed bypurification and fractionation by gel filtration into tow peaks. Elisa inhibition withserum samples from 77 patients who were sensitive to bovine dander, revealedsimilar lgE,lgG binding patterns for each peak. Also, there is a cross —reaction .between these two peaks.PeakII exhibited the highest IgE binding inhibition andshowed a specific allergenic activity about two fold higher than that of peakI.Theallergenecity ofpurified allergen was not lost during purification process.

Keywords

Elisa --- Dander --- IgE


Article
The Human Seroprevalence of Echinococcus Granulosus in Sulaimani Governorate

Author: Mohammed Omer Mohammed
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2013 Volume: 12 Issue: 1 Pages: 45-50
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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ABSTRACT:BACKGROUND:Hydatidosis is one of the major zoonotic diseases that cause considerable economic losses and publichealth problems worldwide. This study was conducted in order to determine the seropositive aspect ofhydatid disease among people in Sulaimani.OBJECTIVE:To determine the Seroprevalence of hydatid disease among people in Sulaimani and it’s relation withage, Gender, occupation and education level.MATERIALS AND METHODS:Two areas of Sulaimani governorate (the Sulaimani city center and Saedsadq as a rural area) wereinvestigated from April to October 2007. In Sulaimani 372 subjects, 248 were females and 124 weremales. In Saedsadq 164 subjects, 78 female and 86 males were randomly collected.A Seroepidimiological survey was conducted by using two methods; a- enzyme-linked immunosorbentassay (ELISA), and b- Indirect Haemagglutination (IHA) test to detect anti Echinococcus granulosusantibody. There were no significant differences between the Seropositivity by the two tests (P > 0.05).RESULTS:The Seropositivity in Saedsadq was (3.7%) higher than in Sulaimani (2.4%). The Seropositivity infemales (2.8%, 6.4%) was higher than in male (1.6%, 1.2%) both in Sulaimani and Saedsadqrespectively.There was no statistically significant difference between different age groups.There were no significant differences (P> 0.01) between the seropositive cases and differentoccupations in both areas.Our results implied that the prevalence of Cystic Echinococcosis (CE) had significant relationship withthe level of education. Among 15 infected persons in Sulaimani and Saedsadq 9(60%) were illiterate.CONCLUSION:Seropositivity for hydatid disease was significantly higher in Saedsadq (3.7%) than Sulaimani city(2.4%), also it was significantly higher among female, housewife, and illiterate persons.


Article
Screening of Human Cytomegalovirus (CMV) in Diabetic Patients in Najaf Governorate

Authors: Zainab Hemeed Al-Heidery --- Heider H. Abbas --- Saif Jabbar Yasir
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2013 Volume: 10 Issue: 1 Pages: 236-244
Publisher: Babylon University جامعة بابل

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Abstract

The aim of current study was to study the relationship between the virus and diabetes mellitus through detection of human cytomegalovirus (HCMV) infection in diabetic patients in Najaf governorate by screening of anti-human cytomegalovirus IgM antibodies in the serum of diabetic patients by using of ELISA technique and detection of human cytomegalovirus DNA in blood samples by using of PCR technique. Blood samples collected from 140 diabetic patients randomly. ELISA technique was used to know the activity of humeral immunity among patients through detection of anti-HCMV IgM antibodies, where the result showed that IgM profile was positive in 49 patients(35 %). Regarding PCR technique, the study showed that among 140 diabetic patients gave positive results in 27 patients(19.2%); In contrast, the results of control group were negative for anti-HCMV IgM antibodies in both ELISA and PCR technique. This study concluded that the higher prevalence of seropositivity for human CMV in diabetic patients comparing with normal individuals which means that cytomegalovirus patients with diabetic were at high risk for CMV infections. A higher prevalence of CMV antibodies was observed in diabetic patients of all age-groups as compared with control group.

لقد كان هدف الدراسة معرفة العلاقة بين فايــــــروس (سي أم في) و مرضى السكري(مجموعة الدراسة) من خــلال التحــــري عن وجود الاصابة بالفايــــــــروس في مرضى السكري في محافظة النجــــــف بواســـــطة اجراء مســح لوجود الاجسام المضادة نوع( آي جي أم) في مصول مرضـــــــى السكري باستخدام تقنيــــــــــة (الاليزا), وكذلك من خــلال اجراء مسـح لوجود الحامض النــــووي(دي أن أي) لفايروس (سي أم في) في عينات دم نفس المــرضى باستخدام تقنية (بي سي آر) . تم خلال الدراسة جمع 140عينة دم بصورة عشوائية من مرضى السكري في مدينة الصدر الطبية في النجـــف. لقد استخدمت تقنية (الالـــــيزا) لمعرفة فعالية المناعة الخلطية بين المرضى من خلال كشـف وجود الاجسام المضـادة نوع (آي جي أم) والتي كانت نتيجته وجود 49 حالة موجبة (35 %) بينما اظهرت نتائج الدراســــة باســتخدام تقنيــة (بي سي آر) وجود 27 حالة موجبة (19.2 %) بينما أظهرت نتائـــج الدراســـــــة عدم وجود أي حالة موجبـــة في مجموعة السيطرة باستخدام كلا التقنيتين. لقد اظهرت الدراسة أن انتشـــــــار فايروس (سي أم في) في مجموعة الدراسة (مرضى السكري) كان أعلى بالمقارنة بمجموعة السيطرة مما يعني أن مرضى السكري أكثر خطورة للإصابة بفايروس (سي أم في) كما لوحظ أن وجود الاجســام المضادة لفايروس في دم مرضى السكري( مجموعة الدراسة) كان أكثر انتشارا في جميع الفئات العمرية مقارنة بمجموعة السيطرة

Keywords

HCMV --- Diabetic --- ELISA --- PCR.


Article
MOLECULAR AND SEROLOGICAL IDENTIFICATION OF FOOT-AND-MOUTH DISEASE VIRUS SEROTYPES IN CATTLE OF BASRAH PROVINCE
التشخيص الجزيئي والمصلي للانماط المصليه لفايروس مرض الحمى القلاعيه في ابقار محافظة البصره

Author: Adnan M. Al-Rodhan Zainab M. Salem عدنان موسى الروضان زينب مجيد سالم
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: 18138497 Year: 2014 Volume: 13 Issue: 1 Pages: 180-197
Publisher: Basrah University جامعة البصرة

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Abstract

This study was conducted on serum from 6 different regions covering all parts of the Basrah province during the period from October 2012 to June 2013 in order to determine the prevalence of foot and mouth disease in bovine species. From the serology results, the overall prevalence of this disease was(72.7%.) . Of the 165 samples subjected to Nonstructural protein (NSP) enzyme linked immunosorbent assay (ELISA) screening test, 120 were interpreted as positive representing (72.7%.) while the other 45 samples were negative representing 27.3%. The seropositivity significantly associated with age groups (p<0.01) and sex (P < 0.05). RT-PCR base detection of FMDV for primary and serotype specific diagnosis was used. Different types of clinically positive samples of FMD were collected from the cattle which includes samples from mouth vesicles, serum and saliva .RT-PCR based identification was done with( universal primer sets 1F / 1R, A- 1C562, O-1C272 and As1-1C505) with expected band of( 328, 866, 639 and 911 bps.respectively) . Of eighty three samples collected, only 68(81.9%) were successfully amplified by RT-PCR, and of these, only 19(22.9%) samples were found to be serotype A-1C562 positive and 26(31.3%) were serotype O-1C272 positive and nil for serotype As1-1C505. The frequency of foot-and-mouth disease viral genome presence in cattle had no significant differences based on sex (P>0.05) while the difference among age groups was significant (P < 0.05)

اجريت هذه الدراسه في6 مناطق مختلفه ممثله لجميع اجزاء محافظة البصره خلال الفتره من شهر تشرين الاول من العام 2012الى شهر حزيران من عام 2013 على عينات من المصل وذلك لتحديد نسبة انتشار مرض الحمى القلاعيه في الابقار.من نتائج الفحص المصلي ان النسبه الكليه لانتشار هذا المرض كانت72.7%.من 165عينه خضعت لاختبار الممتز المناعي المرتبط بالانزيم المعتمد على مستضد البروتين اللا تركيبي تاكدت ايجابية 120 عينه وتمثل نسبه72.7% بينما كانت 45عينه سالبه وبنسبة .27.3%. ان ارتباط الايجابيه المصليه بالفئات العمريه كان عالي المعنويه من الناحيه الاحصائيه((p<0.01وكذلك مع الجنس كان الارتباط معنويا(P < 0.05). استخدم اختبار سلسلة البلمره المنعكس للكشف عن فايروس مرض الحمى القلاعيه من حيث التشخيص الاولي والخاص بتحديد الانماط المصليه.جمعت عينات سريريه مختلفه ممثله لمرض الحمى القلاعيه من الابقار المريضه والتي تشمل الحويصلا ت الفمويه والمصل واللعاب. ان تشخيص الفايروس بالاعتماد على تفاعل سلسلة البلمره المنعكس تم باستخدام البادئات 1F / 1R)و A- 1C5و O-1C272و As1-1C505)وبحزمه متوقعه (328و866و639على التوالي).من ثلاثه وثمانون عينه ضخمت68(81.9%) عينه فقط بواسطة تفاعل سلسلة البلمره المنعكس ولوحظ ان 19(22.9%)عينه منها كانت موجبه للنمط المصلي A- 1C562وان26(31.3%) عينه منها كانت موجبه للنمط المصلي O-1C272وكانت نتائج النمط المصلي As1-1C505 سالبه في جميع العينات.اعتمادا على الجنس لم تكن نسبة تكرار وجود الحامض النووي الفايروسي معنويه احصائيا (P>0.05) بينما كان الفرق بين الفئات العمريه كان معنويا (P < 0.05).

Keywords

Serum --- Bovine --- ELISA.

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