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Article
A comparative study between Rose Bengal and indirect Enzyme Linked Immunosorbent Assay tests for detection of brucella antibodies in Al-Alum & Begy regions
دراسة مقارنة بين أختباري وردية البنكال والادمصاص المناعي المرتبط بالانزيم غير المباشر في الكشف عن اضداد جراثيم حمى ملطا في أمصال الاغنام في منطقتي العلم وبيجي

Author: Idrees Belal Al-abdaly ادريس بلال علي العبدلي
Journal: Tikrit Journal for Agricultural Sciences مجلة تكريت للعلوم الزراعية ISSN: 18131646 Year: 2012 Volume: 12 Issue: 2 Pages: 41-45
Publisher: Tikrit University جامعة تكريت

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Abstract

أستهدفت الدراسة الحالية تحديد نسبة تواجد اضداد جراثيم حمى ملطا في أمصال اغنام غير ملقحة في مناطق مختلفة من محافظة صلاح الدين، وأجراء مقارنة بين أختباري وردية البنكال والادمصاص المناعي المرتبط بالانزيم غير المباشر. تم فحص 92 عينة (العلم : 48 ، بيجي : 44 ) مصل مثلت 350 رأسا من الاغنام توزعت على منطقتي العلم( 200 راس) وبيجي( 150 راس) . أظهرت نتائج اختبار وردية البنكال أن النسبة الكلية لتواجد الاضداد بلغت (11.95٪) . وسجلت أعلى نسبة في منطقة العلم (12.5 ٪)، بينما سجلت منطقة بيجي (11.36 ٪)، في حين كانت النسبة الكلية لتواجد الاضداد باستخدام اختبار الادمصاص المناعي المرتبط بالانزيم غير المباشر (22.82٪)، وكانت أعلى نسبة في منطقة العلم (25٪) في حين كانت نسبة الاصابة في منطقة بيجي (20.45٪) . أظهرت النتائج أن التوافق بين نتائج الاختبارين كان قليلا اذ بلغت قيمة كابا (0.555) وهذا يدل على حساسية اختبار الادمصاص المناعي المرتبط بالانزيم غير المباشر مقارنة مع أختبار وردية البنكال في الكشف عن أضداد جراثيم حمى ملطا في امصال الاغنام

This study was conducted to determine seroprevalence of brucellosis in non vaccinated sheep in Al-alum & Biji regions in Salah EL-Din and to compare Rose Bengal and indirect ELISA tests for the detection of the antibodies. A total of 92 blood serum samples were examined, representing 350 sheep distributed in the two regions. Results showed that the total seroprevalence of antibodies to brucellosis by using Rose Bengal test was (11.95%), with the highest rate was (12.5%) in Al-Alum followed by (11.36%) in Biji region. Indirect ELISA test showed that the total percentage Of antibody seroprevalence was (22.82٪) and the highest (25٪) was reported in Al-Alum region followed by 20.45٪) in Biji region. The Kappa value between the two tests was (0.555) Higer sensitivity of indirect ELISA test compared with Rose Bengal in brucella antibodies in sheep serum .


Article
Profile of Some Cytokines in Sera of Children with Autism Syndrome
نسق بعض الحركيات الخلوية في مصول أطفال متلازمة التوحد

Authors: Kamil M. Halboot كامل مهدي حلبوت --- Alice K.Melconian أليس كريكور اغوب --- Jenan AL- Saffar جنان محمد جواد الصفار
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2015 Volume: 56 Issue: 1A Pages: 125-131
Publisher: Baghdad University جامعة بغداد

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Abstract

Autism spectrum disorder (ASD) is a spectrum of behavioral anomalies characterized by impairment in social interactions and communication deficits. A potential role for immune dysfunction has been suggested in ASD. To test this hypothesis, certain cytokines: IL-2, IL-10, IL-12, IL-17A and IFN-γ were investigated in serum of all participants. The study includes: 39 child (male and female) aged < 5 to10 years with confirmed diagnosis of autism using standard assessment, age and gender matched 24 confirmed healthy children and 19 non autistic siblings used as controls. Serum was isolated and cytokines were detected using enzyme linked immunosorbent assay (ELISA). The observations indicate a significant increase (P < 0.05) in autistic patients serum levels of IL-10 compared with healthy control, but with lack of significant difference with their related non autistic siblings. Whereas detection of IL-12 and IFN-γ in the autistic patients serum showed significantly decrease level (P < 0.05) compared with healthy control, but with lack of significant difference with their related non autistic siblings. On the other hand, detection of IL-2 and IL-17A results showed no significant (P > 0.05) differences compared with healthy control and non autistic siblings.

أضطراب طيف التوحد (ASD) هو طيف من الشذوذ السلوكي الذي يتميز بضعف في التفاعل الاجتماعي واختلال التواصل. الدور المحتمل للخلل المناعي تم اقتراحه في اضطراب طيف التوحد. ولاختبار هذه الفرضية، أجري فحص مصول جميع المشاركين لتواجد الحركيات الخلوية L-2, IL-10, IL-12, IL-17A ,IFN-γ . شملت الدراسة: 39 طفل ( ذكر وأنثى ) تتراوح أعمارهم اقل من 5 إلى 10 سنوات بتشخيص مؤكد للأصابه بالتوحد وفق تقييم قياسي لمعهد الرحمن لرعاية التوحد، ومجاميع سيطرة بأجناس وفئات عمريه مماثله لإغراض المقارنة مكونه من ( 24 طفل سليم و 19 طفل من الأخوة غير المصابين بالتوحد ). تم قياس تركيز الحركيات الخلوية IL-2, IL-10, IL-12, IL-17A,IFN-γفي مصول دم المشاركين باستعمال تقنية الأمتزاز المناعي المرتبط بالإنزيم (ELISA). أظهرت النتائج زيادة معنوية (P < 0.05) لمستوى IL-10 في مصول مرضى التوحد عند مقارنتهم مع الأطفال الأصحاء ، ولكن عدم وجود اختلاف معنوي مع أشقائهم غير المصابين بالتوحد. بينما الكشف عن مستويات IL-12 و IFN-γ في مصل أطفال التوحد أظهرت انخفاض معنوي (P < 0.05) عند مقارنتهم مع الأطفال الأصحاء، وعدم وجود اختلاف معنوي مع أشقائهم غير المصابين بالتوحد، بينما الكشف عن مستوى IL-2 و IL-17A أظهرت النتائج عدم وجود اختلاف معنوي عند المقارنة مع مجموعة السيطرة والأخوة غير المصابين.


Article
A Study of the Relationship Between CMV IgG Titers and Blood Pressure in Iraqi patients
دراسة العلاقة بين مستويات الاجسام المضادة من نوع IgG لفايروس CMV وارتفاع ضغط الدم في المرضى العراقيين

Author: Maryam Kamel Mohammed مريم كامل محمد
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2016 Volume: 57 Issue: 1B Pages: 325-329
Publisher: Baghdad University جامعة بغداد

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Abstract

Human cytomegalovirus (CMV) infection is associated with higher risk of cardiovascular diseases in immunocompromised organ transplant patients; it has been linked with the elevated arterial blood pressure. This study aimed to find a relationship between CMV antibody titers and blood pressure elevation by using the enzyme-linked immunosorbent assay (ELISA) to measure CMV IgG levels in the serum of 60 patients with high blood pressure, in comparison to 30 healthy persons with normal blood pressure as a control. All patients and control were 25-50 years old. The results showed that CMV antibody titers were higher in those who undergo blood pressure elevation. This finding supports the hypothesis that; the common CMV infection may leads to impaired vascular function

لُوحظ وجود علاقة بين اصابات فايروس الـ CMV و امراض القلب في المرضى الخاضعين لعمليات نقل الاعضاء من ذوي المناعة المنخفضة مما يؤدي الى الاعتقاد بوجود علاقة اخرى بين الاصابة بهذا الفايروس وحدوث حالات ارتفاع ضغط الدم ، لذا يهدف هذا البحث لدراسة تلك العلاقة بين مستويات الاجسام المضادة من نوع IgG عند الاصابة بـفايروس CMV وحالات ارتفاع ضغط الدم من خلال قياس مستوى او معدل هذه الاجسام المضادة في مصل 60 مصاب بالفايروس بواسطة طريقة الـ ELISA مقارنةبعدد السيطرة البالغين30 شخص طبيعي ليس له تاريخ مرضي او ارتفاع ضغط الدم وقد تراواحت اعمار الافراد قيد الدراسة (25-50) سنة، حيث اكدت النتائج العلاقة بين وجود هذه الاجسام المضادة وحدوث حالات ارتفاع ضغط الدم مما يؤكد نظرية خطورة الاصابة بفايروس CMV على وظائف القلب والشرايين .


Article
Evaluation of markers CD4+, CD8+, IFN-γ in male smokers in diayla governorate / Iraq
تقييم المؤشرات +IFN- γ ,CD8+ , CD4 عند المدخنين الذكور في محافظة ديالى / العراق

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Abstract

Background: Cigarette smoking habit is widely distribute in the whole world, obviously this bad habit responsible on many diseases which affect the general personal health of the smokers through its effect on the organs of the body in general and the immune system in particular.Objective:To determination of the smoking effect on some immunity parameters and compare the results with the non-smokers by using IFN-γ, CD4+and CD8+.Patients and Methods: 45 of smokers blood samples and 44 of non-smokers blood samples are collected. The levels of cellular of IFN-γ,CD4+and CD8+has been determined by using sandwich ELISA test.Results: The study included 45 smoker that have the average age of 29.60 ( ± ) 10.36 year, while the healthy non-smokers group have 44 person who's have the average age of 26.56 ( ± ) 9.09 years old. When comparing the level of CD4+T cells ,CD8+T and the level of IFN-γwith the number of cigarettes consumed per day, the class (20-1) was found to be the highest average of T cells.while (40-21) were less than average, with no significant difference. When comparing the level of CD4+T cells and the level of CD8+T cells with IFN-γwith smoking duration, the highest mean T-cell (10-6) years and the lowest mean (25-21). The results of the present study showed a statistically significant association between CD4+, CD8+and IFN-γmolecules.Conclusion: The general results of the tests on the effect of smoking on immunologic markers CD4+, CD8+and IFN-γassumed an increase in the ratio of the three indicators compared to the control group.

خلفية الدراسة: ظاهرة التدخين منتشرة على نطاق واسع في جميع انحاء العالم وعادة تدخين السجائر تضر كل اعضاء الجسم تقريباً وتسبب العديد من الامراض وتقلل الصحة العامة للشخص المدخن من خلال تأثيرها على أجهزة الجسم بصورة عامة والجهاز المناعي بصورة خاصة .اهداف الدراسة: هدفت هذه الدراسة إلى معرفة تأثير التدخين على بعض المؤشرات المناعية للمدخنين ومقارنة ذلك مع غير المدخنين عن طريق قياس المؤشرات الحيوية المناعية الاتية IFN-γ و CD4+ و CD8+ .المرضى والطرائق: تم جمع ((45 عينة دم من الاشخاص المدخنين و (44) عينة دم من الاشخاص غير المدخنين و تم تحديد مستوى الحركيات الخلوية IFN-γ و CD4+ و CD8+ كمياً بأستخدام اختبار الادمصاص للأضداد المناعية المرتبط بالأنزيم Sandwich ELISA test)) .النتائج: شملت الدراسة الحالية 45 مدخن وبمتوسط عمري 10.39) ± (29.60 سنة , اما المجموعة الضابطة فتضم 44 غير مدخن من الاصحاء ظاهرياﹰ بمتوسط عمري (26.56 ± 9.09) سنة مع عدم وجود فرق معنوي.عند مقارنة مستوى الخلايا التائية المساعدة CD4+و مستوى الخلايا التائية السمية CD8+ و مستوى IFN-γ مع عدد السجائر المستهلكة في اليوم الواحد وجد ان الفئة (20-1) سجارة تشكل اعلى متوسط للخلايا التائية المساعدة بينما الفئتان (40-21 , 40≤) سجارة شكلتا اقل متوسط مع عدم وجود فرق معنوي . عند مقارنة مستوى الخلايا التائية المساعدة CD4+و مستوى الخلايا التائية السمية CD8+ و مستوى IFN-γ مع مدة التدخين وجد ان اعلى متوسط للخلايا التائية المساعدة في الفئة (10-6) سنة واقل متوسط في الفئة (25-21) سنة مع عدم وجود فرق معنوي. أظهرت نتائج الدراسة الحالية وجود ارتباط احصائي ذي دلالة معنوية عالية بين جزيئات +CD4 و CD8+ و IFN-γ .الاستنتاجات: افترضت النتائج العامة للأختبارات تأثير التدخين على المؤشرات المناعية +CD4 و CD8+ و IFN-γ وجود ارتفاع في نسبة المؤشرات الثلاثة مقارنة مع مجموعة السيطرة .


Article
Evaluation of Performance Characteristics of Commercially Available Tests for Diagnosing Hepatitis B Surface Antigenemia

Authors: Saad Hasan Mohammed Ali --- Saja Jehad Al-Khalidi --- Waleed Nadi Kasim
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2009 Volume: 8 Issue: 2 Pages: 159-171
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGROUND:A number of serologic immunoassays techniques have been developed in diagnostic virology with different degrees of sensitivity and specificity for the detecting hepatitis B virus (HBV) antigens and their relevant antibodies.OBJECTIVE:This study was designed to apply and assess the sensitivity and specificity of different commercially available laboratory techniques for detecting hepatitis B surface antigen(HBsAg).METHODS:One hundred and twenty-one sera samples were collected from National Center for Blood transfusion, Gastroenterology and Liver Diseases Hospital, Central Public Health Laboratories and Teaching Laboratories. According to the manufacturer’s practical instructions, a group of commercially available laboratory methods for detecting HBsAg were applied , including enzyme linked immunosorbent assay (ELISA), enzyme linked fluorescent assay(ELFA), immunochromatographic assay(ICA), and latex agglutination test(LAT).RESULT:Among ELISA, ELFA, ICA, and LAT methods for detecting HBsAg, the 3rd generation ELISA was proved to have very high specificity (no false negative results) and the least one that has necessitated few confirmatory repetitions. ELFA versus ELISA has showed relatively lower sensitivity (more false negative results). However, similar to ELISA, ELFA showed very high specificity. Immunochromatographic assay (ICA) confidentially appeared to be a good, rapid and simple technique with comparable sensitivity and specificity to ELISA and ELFA techniques. Although LAT was introduced as a rapid, simple and cheap technique for HBsAg screening, it showed frank lower sensitivity and specificity that deranged it from competing with all those tested techniques.CONCLUSION:The concomitant use of ELFA with ELISA compensates its relatively lower sensitivity in front of ELISA. Latex agglutination test for HBsAg has relatively lower sensitivity and specificity than all other tests. For its comparable performance characteristics to ELISA, the use of ICA is ideally suited for HBsAg screening, in respect to its lower cost, rapidity, simplicity and no need for expensive equipments


Article
Dental caries and salivary physiochemical characteristics among osteoporotic old adult women

Authors: Hiba F. Al-Sekab --- Ban S. Diab بان صالح ذياب
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2014 Volume: 26 Issue: 3 Pages: 122-128
Publisher: Baghdad University جامعة بغداد

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Background: Osteoporosis is a systemic skeletal disorder affects general health in addition to effect on salivaryphysical and chemical composition which lead to an adverse effect on oral health status. This study was conductedto evaluate the concentration of glycoprotein (osteonectin) in saliva and its effect on dental caries in relation tosalivary flow rate and viscosity among osteoporotic women and compared to control group.Materials and Methods: The total sample composed of 60 females aged 60-65 years attending Al-Yarmook TeachingHospital, 30 females diagnosed with osteoporosis by measuring bone density at the spine and femur with a dualenergyX-ray absorptiometry at T-score of >2.5, and 30 women without osteoporosis with T-score of -1 (controlgroup).The diagnosis and recording of dental caries was through the application of D1-4MFS index according tocriteria of Mühlemman (1976). Stimulated salivary samples were collected under standardized condition, accordingto Tenovuo and Lagerlöf, (1994). The flow rate and viscosity were estimated and then the saliva was analyzed forestimation of glycoprotein (osteonectin) by using Enzyme-linked immunosorbent assay (ELISA).Results: The percentage of dental caries occurrence was 100% among osteoporotic group and control group.Results revealed that DMFS value was higher but statistically not significant among osteoporotic women, ConcerningDMFS components, the data of the present study showed that the DS was lower but statistically not significantamong osteoporotic women, while MS value was significantly higher among them (t=2.044, P<0.05, df =58), on theother hand the opposite figure was found concerning filling component FS however the difference was notsignificant.Correlation coefficients of caries experience with salivary flow rate revealed a weak negative not significantcorrelation with D1-4MFS and its component.On the other hand, the data of the present study showed that salivaryviscosity correlate weakly not significant in negative direction with DS and its severity and in positive direction withMS, FS, DMFS.. The correlation coefficient between salivary osteonectin and dental caries were weak significant inpositive directions concerning Ds and its severity except D3 and FS component as the relation were in negativedirection, the positive not significant relation were also found between salivary osteonectin and MS,DMFS.Concerning the relation between salivary osteonectin and physical properties of saliva, the data of the present studyrevealed a non-significant relation in negative direction with salivary flow rate and in positive direction for salivaryviscosity.Conclusions: Dental caries revealed lower percentage of occurrence among osteoporotic group


Article
The effect of cigarette smoking on salivary IgA and periodontal disease

Author: Omar Husham Ali عمر هشام علي
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2015 Volume: 27 Issue: 3 Pages: 116-119
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Chronic periodontitis is an inflammatory disease of tissues supporting the teeth. Salivary compositionshave been most intensely studied as a potential marker for periodontal disease. In this study, analysis of salivaprovides a simple and non-invasive method of evaluating the role of salivary IgA (s-IgA) levels in periodontal diseaseby detecting the level of (s-IgA) in patients with chronic periodontitis smokers and non smokers patients and correlatethe mean (s-IgA) levels with clinical periodontal parameters Plaque index (PLI) gingival index (GI), probing pocketdepth (PPD) and clinical attachment level (CAL).Materials and Methods: The study samples consists of (15) patients with chronic periodontitis who were non smokers(Group I) and (15) patients with chronic periodontitis who were smokers (Group II) of both gender with an ageranged (35-45) years were the periodontal parameters used in this study (PLI, GI, PPD and CAL), unstimulated salivarysample were collected from all subjects and the levels of salivary IgA (s-IgA) in each sample were analyzed for eachgroup by using enzyme-linked immunosorbent assay (ELISA) technique. A statistical analysis was done by using excel2013.Results: There was a significant difference with high mean level in the clinical periodontal parameters in smokersgroup compared to non smokers with chronic periodontitis (PLI, PPD and CAL) except GI which showed nosignificant difference between the same groups. The biochemical finding showed significant difference with lowmean level for (s-IgA) in smokers group compared to non smokers.Conclusion: The findings in this study showed that the concentrations of salivary IgA might be used as an indicator forperiodontal disease progression in smokers with chronic periodontitis as a resultant to the effect of smoking whichlowering the concentration of the salivary IgA and subsequent reducing of the host’s defense lead to increase in theprogression of periodontal disease


Article
Serosurvillance on Toxoplasmosis in Camels (Camelus dromedarius) at Al-Najaf Province

Authors: Mahmoud, Marai H. S --- Al-Rubaie ,Abdel-Elah S.M. --- Al-Jeburii, Kefah O. S --- Taha, Abdel-Kareem A.
Journal: Kufa Journal For Veterinary Medical Sciences مجلة الكوفة للعلوم الطبية البيطرية ISSN: 20779798 Year: 2014 Volume: 5 Issue: 2 Pages: 204-210
Publisher: University of Kufa جامعة الكوفة

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Abstract

The current study was conducted to evaluate the seroprevalence of toxoplasmosis in camels resident in Al-Najaf province in Iraq . Serum samples were collected from 360 camels, 219(60.83%) of samples represents males and 141(39.16%) were collected from females. Latex Agglutination Test(LAT) was applied to screen all serum samples for Toxoplasmosis while ELISA was also used to confirm the positive result obtained by LAT Using LAT, out of 360 serum samples 91 (25.2% ) were positive to Toxoplasma gondii. Percentage of positive cases was more in females (30.4 %) than it is in males (21.9 %); the percent of seropositivity increased by increasing animals age. The ELISA test showed that 15 (16.4 %) samples were positive for toxoplasmosis, the males were 7 (14.5 %)which was lower than the percentage in females 8 (18.6 %). Also the percentage of positivity increased with increasing animals age.


Article
Association of sFas and sFas Ligand with Progression of Type 2 Diabetes Mellitus in Basrah Province

Author: Saja Majid Hamid, Wafaa Sadoon Shani
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2018 Volume: 15 Issue: 2 Pages: 135-138
Publisher: Babylon University جامعة بابل

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Abstract

Objective: This study aimed to assess the role of Fas/Fas ligand (FasL) system in the progression of type 2 diabetes mellitus (T2DM).Materials and Methods: This study included 100 participants – 30 persons as a control group and 70 patients with T2DM (35 males and 35 females)and their ages were ranged from 40 to 70 years. The patients were distributed into two groups according to gender and duration of the disease:newly diagnosed group for short duration ≤5 years and chronic diagnosed group for long duration >5 years. Serum sFas and sFasL levelswere measured by enzyme‑linked immunosorbent assay technique, and also, lipid and glucose profile were measured by COBAS analyzer.Results: The results revealed a significant (P ≤ 0.05) decrement in the levels of FasL in T2DM than controls while the levels of Fas wereincreasing significantly (P ≤ 0.05) in T2DM than controls. Hemoglobin A1c (HbA1c) and fasting blood glucose were negatively correlatedwith FasL, while high‑density lipoprotein was positively correlated with it, and whereas HbA1c positively correlated with Fas, the genderand duration of disease did not show any correlation with the disease. Conclusion: Our findings suggest that hyperglycemia causes increasein Fas levels which lead to dysfunction of pancreatic β‑cell in T2DM.


Article
Non-organ Specific Autoantibodies (ANA & SMA) in Type 1-Autoimmune Hepatitis
الأضداد الذاتية غير المناوعة لعضو( اضداد النواة والعضلات الملساء) في التهاب الكبدالمناعي الذاتي- النمط الأول

Author: Samira N. Al-Naaiem د.سميره نعيم النعيم
Journal: IRAQI JOURNALOF COMMUNITY MEDICINE المجلة العراقية لطب المجتمع ISSN: 16845382 Year: 2009 Volume: 22 Issue: 2 Pages: 92-96
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Background: Autoantibodies against nuclear antigen and smooth muscle characterize type 1-autoimmune hepatitis. Antinuclear antibodies directed against functional and structural component of the cell while smooth muscle antibodies exhibit reactivates against actin and Non-actin components. Objective: Aim of this study is to evaluate the prevalence and clinical relevance of antinuclear antibodies and smooth muscle antibodies in autoimmune hepatitis -1. Methods: The study was performed on 73 Iraqi patients with chronic active hepatitis of unknown cause, attending the teaching hospital for gastroenterology and liver disease in a period from November 2006 through July 2007. : Anti-nuclear antibodies and anti-smooth muscle antibodies were measured by Enzyme Linked Immunosorbant Assay and Indirect Immunofluorescence technique.Results: Antinuclear and Sooth muscle antibodies were detected in the sera of 49 patients (67.0%) with autoimmune hepatitis, but never in the sera of healthy group just in one patients (0.5%) in comparison to Auto immune hepatitis patients with the frequency (30.6%) was highest among the age group of 30-39 years. Conclusion: Antinuclear Antibodies and Smooth muscle antibodies are present in identified patients with autoimmune hepatiti-1, smooth muscle antibodies are more prevalent than antinuclear antibodies in this type and patients with this type have a bimodal age distribution. Key words: Antinuclear antibodies, anti-smooth muscle antibodies, autoimmune hepatitis type-1, Enzyme Linked Immunosorbant Assay, Indirect Immunofluorescence Technique

الخلفية: تشكل الأضداد الذاتية لمستضدات النواة و العضلات الملساء الميزة لألتهاب الكبد المناعي الذاتي- النمط الأول. توجه اضداد مستضدات النواة ضد مكونات الخلية الوظيفية و التركيبية بينما تظهر أضداد العضلات الملساء فعلا مضاداً للمكونات الأكتينية وغير الأكتينية.هدف البحث: تهدف هذه الدراسة الى تقييم أضداد مستضدات النواة و و العضلات الملساء ومدى انتشارها و تأثيرها في مرض التهاب الكبد المناعي الذاتي.طرق العمل: تم إجراء هذه الدراسة على 73 مريضاً يعانون من التهاب الكبد المناعي الذاتي النشط المزمن غير معروف السبب الراقدين و المراجعين لمستشفى الجهاز الهضمي و الكبد التعليمي خلال الفترة من تشرين الثاني / 2006 الى تموز / 2007 . أستخدمت مقايسة الأنظيم المرتبط الممتز المناعية و تقنية التألق المناعي غير المباشر في الكشف عن أضداد مستضدات النواة و العضلات الملساء .النتائج: تم الكشف عن هذه الأضداد في 49 عينة (67.0%) من أصل 73 عينة لمرضى يعانون من التهاب الكبد المناعي الذاتي في حين أعطت عينة فقط (0.5%) من الأصحاء نتائج موجبة . كذلك ظهرت اعلى نسبة لهذا المرض (30.6%) ضمن الفئة العمرية (30-39) سنة.الأستنتاجات: يستنتج من ذلك أن أضداد مستضدات النواة و العضلات الملساء توجد لدى مرضى التهاب الكبد المناعي الذاتي النمط الأول ، و أن أضداد العضلات الملساء اوسع انتشاراً من أضداد مستضدات النـواة في هذا النمط من المرض حيث يظهر المرضى بهذا النمط توزيعاً عمرياً ثنائي الصيغة أو الموديل أي قمتين لفئتين عمرية.

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