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Article
The In Situ Hybridization Expression of Fas and Fas Ligand (FasL) in Trophoblastic tissue of Aborted Women Compared with Normal Pregnancy.

Authors: Dhamraa W. Ahmad *** MSc --- Dalya B. Hana ** MSc --- Amal H. Salman * PhD
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2009 Volume: 51 Issue: 2 Pages: 211-215
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Estimation of the in situ expression of Fas and Fas ligand (FasL) in aborted women compared with normal human pregnancy .Patients and Methods: A technique utilizing in situ hybridization(ISH) was performed to detect and determine the in situ expression of Fas and Fas ligand (FasL) mRNA using paraffin embedded sections of curettage samples obtained from 42 women, who were divided into two groups: 30 women with first trimester abortion and 12 women with induced abortion as control.Results: The levels of the in situ expression of both Fas and Fas ligand (FasL) mRNA were found to be highly significant increased in group 1 as compared with group 2 (p<0.01), with a significant positive correlation between Fas; and abortion in group I , FasL & abortion in group I , and between these two parameters(Fas & FasL) (p<0.01) in group1 .Conclusions: The increasing expression of Fas and FasL trophoblasts might influence pathogenesis of first trimester abortion.


Article
FAS and FASL genes polymorphisms and their relationship with the incidence of severe oligozoospermia in a sample of Iraqi patients.

Author: Ghassan.M. Hassan1 . Ismail. A. Abdul-Hassan2
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2017 Volume: 16 Issue: 4 Pages: 60-68
Publisher: Baghdad University جامعة بغداد

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Abstract

The interaction between Fas and FasL plays an important role in triggering the apoptotic pathway. Both Fas and FasL exist as membrane bound and soluble forms. It is suggested that Fas may be a marker of overall apoptosis triggering, at the same time regulating apoptosis by competing with the cell surface receptor. The aim of the present study was to detect the FAS and FASL gene polymorphisms and to demonstrate their relationship with incidence of severe oligozoospermia in a sample of Iraqi patients. Following semen analysis, blood samples were collected from severe oligozoospemic patients (n=50) and normozoospermic subjects (control, n=50). DNA was extracted using DNA extraction Kit (Geneaid Biotech). The genotypes of FAS (-670G/A) and FASLG (-844T/C) were determined by using Taqman (RT-PCR) Kit ( WizPure™ qPCR Master ( PROBE) , south Koria). The results of the present study, as related with Fas -670G to A SNP, indicated that the frequencies of both GA and AA genotypes were significantly (p<0.01) higher in severe oligozoospermia group than in normozoospermia group (30% versus 20% and 60% versus 0%, respectively). Also, as related with FasL -844C to T SNP, the frequencies of both CT and TT genotypes were significantly (p<0.01) higher in severe oligozoospermia group than in normozoospermia group (38% versus 16% and 50% versus 0%, respectively). In conclusion, there was an A allele-related risk factor for Fas -670G to A SNP and T allele-related risk factor for FasL -844C to T SNP with the incidence of severe oligozoospermia in Iraqi patients.


Article
muSerum soluble Fas in Hodgkin's disease

Authors: Abbas H. Abdulsalam --- Subh S. Al-Mudalal
Journal: Iraq Joural of Hematology المجلة العراقية لامراض الدم ISSN: 20728069/25432702 Year: 2011 Volume: 1 Issue: 1 Pages: 22-26
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Abstract:Background: Hodgkin disease (HD) is a histologically defined B- cell neoplasm and it includes two distincttypes of disease, classical and nodular lymphocyte-predominant Hodgkin’s disease. Disruption of thephysiological balance between cell proliferation and apoptosis is a universal feature of all cancers.Apoptosis is caused by activation of the caspases through extrinsic and intrinsic pathways. Extrinsicpathway centers on TNF family, where the ligand will bind to the cell surface receptor to induceapoptosis. Fas receptor is a member of the TNF receptor superfamily. Fas family is constituted of thereceptor, ligand and soluble form. Soluble Fas will interfere with apoptosis by competing with Fasreceptor for binding to legend. The aim of this study is to measure the concentration of serum soluble Fasin Hodgkin’s disease patients and to determine the relation between it and certain clinical parameters andserum markers. Also to compare the concentration of serum sFas in HD and Non-Hodgkin’s lymphoma(NHL) patients.Patients & Methods: This study included 15 patients with Hodgkin's disease, 9 males and 6 females. Thepatients were interviewed for history and clinical examination and blood was collected for measurementof serum sFas concentration using ELISA technique. Hemoglobin (Hb) concentration, leucocytes andplatelets counts, peripheral blood smear, C-reactive protein (CRP) and lactate dehydrogenase (LDH) levelwere all performed using standard techniques.Results: This study revealed that the serum soluble Fas concentration was almost the same (p-value = 0.991) inboth HD patients (1996.9 ± 131) and normal controls (1993 ± 125.9). However, by comparing the resultsof this study with the high concentration results obtained for serum sFas in newly diagnosed NHLpatients (6475.9 ± 617) of another study, it revealed a highly significant difference (p-value < 0.0001)between the two groups.Conclusions: The serum Fas concentration differs significantly between HD and NHL patients but not withcontrol patients. Further studies including larger number of patients are recommended.


Article
Expression of death receptor (Fas) , and it's specific ligand (FasL)in transitional cell carcinoma of the urinary bladder.
تعبير مستقبل الموت FAS ورابطه النوعي FAS LIGAND في الخلايا السرطانية للمثانة البولية

Authors: مي خليل اسماعيل --- غنيمة صادق محمد --- خالد طارق النائب
Journal: journal of kerbala university مجلة جامعة كربلاء ISSN: 18130410 Year: 2013 Volume: 1 Issue: المؤتمر العلمي الاول لكلية العلوم Pages: 98-105
Publisher: Kerbala University جامعة كربلاء

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Abstract

This study was designed by using 36 paraffin embedded tumor tissues obtained from TCC patients, and 10 normal bladder tissues obtained from forensic medicine institute. All specimens undergone to in situ hybridization technique for in situ mRNA expression detection of Fas and Fas L ; results of this study reveled that a high significant expression in malignant tissues compaired with normal tissues (p< 0.05 ), (P > 0.05) respectively .Moreover ,FasL expression on transitional malignant cells has a linear increase with tumor grade and stage progression ; there was a significant difference in FasL expression between grade I and grade III(P < 0.05) and between stage T1 and T2 (P < 0.05) . while we found an inverse relation between Fas expression on these cells and tumor grades and stages progression ; there was a significant difference among grades and among stages (P < 0.05) , (P < 0.05) respectively. while there was no significant differences in Fas /FasL expression on lymphocytes and tumor grade and stage progression (P>0.05), (P>0.05) respectively. A highly significant liner relation was showed between Fas L expression on transitional malignant cells tumor grade and stage progression (p<0.01) , (p<0.01) respectively .From this study we conclude that overexpression of FasL are correlated with TCC invasion and progression.

صممت هذه الدراسة باستخدام36 نموذجاً نسيجياً مطموراً بشمع البرافين مأخوذاً من مرضى مصابين بسرطان الخلايا الانتقالية في المثانةTransitional cell carcinoma( TCC ) 27مريضا كانوا من الذكور و 9 من الاناث تراوحت اعمارهم بين 35 و97 سنة , ووفقا الى التقارير المرضيه النسيجية صنفت الانسجة السرطانية الى درجاتgrade ) І , І І , І І І(وكل درجة ضمت 12 خزعة سرطانية , كما صنفت الى مراحل stages (Ta, T1, T2) تحتوي كل مرحلة على(3 ,25 ,8) خزعة سرطانيه على التوالي بالاضافة إلى 10 نماذج نسيجية طبيعية مأخوذة من معهد الطب العدلي تعود ل6 ذكور و4 اناث تراوحت اعمارهم بين 36 و65 سنة. خضعت جميع النماذج لتقنية التهجين الموضعي in situ hybridization لغرض التحري عن التعبير الموضعي للحامض الرايبي الرسول mRNA لكل من مستقبل الموت Fas ورابطه النوعي Fas ligand . أظهرت نتائج هذه الدراسة إن نسبة إصابة الذكور إلى الإناث بـسرطان الخلايا الانتقالية هي 1:3 وأن معدل أعمار المصابين كان 60 سنة .واعتماداً على نتائج التهجين الموضعي لكل من مستقبل الموت Fas ورابطه النوعي FasL ظهرت زيادة معنوية في التعبير عنهما في الانسجة السرطانية عندما قورنت بالأنسجة الطبيعية (P<0.05) و(P<0.05) بالتتابع كما لوحظ ان تعبير الخلايا السرطانية الانتقالية عن FasL يتناسب طردياً مع تقدم كل من درجة الورم ومرحلته Tumor grade and stage فهناك فرق معنوي في التعبير عن FasL بين الدرجة الاولى والثالثة (P<0.05) وبين المرحلة T1 وT2(p<0.05 ) .اما تعبير هذه الخلايا عن المستقبل Fas فقد لوحظ انه يتناسب عكسياً مع تطور درجات الورم ومراحله فهناك فرق معنوي في تعبير هذه الخلايا عن Fas بين درجة وأخرى (p<0.05) وبين مرحلة وأخرى (p<0.05) . في الوقت الذي لم يظهر فيه تعبير الخلايا اللمفاوية عن Fas وFasL اختلافاً مع تطور درجات الورم ومراحله(P>0.05) و(P>0.05) بالتتابع . نستنتج من الدراسة ان التعبير المفرط للخلايا السرطانيه عن الرابط النوعي لمستقبل الموت FasL له علاقه بتقدم اورام الخلايا الانتقالية للمثانة البوليه واجتياحها .


Article
EXPRESSION OF Fas AND FasL IN TROPHOBLASTIC TISSUE OF WOMEN WITH SPONTANEOUS AND INDUCED ABORTION USING INSITU HYBRIDIZATION TECHNIQUE

Authors: Abdul-Razzak H. Ahmed عبد الرزاق حردان احمد --- Mohammed R. Ali محمد رزاق علي
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2011 Volume: 9 Issue: 2 Pages: 147-151
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

BackgroundApoptosis of trophoblastic cells may play a role in the pathogenesis of abortion, and one mechanism of apoptosis is Fas receptor and legand system.ObjectiveEstimate the level of Fas and FasL in trophoblastic tissue of aborted women.MethodsIn this study, 25 women with spontaneous abortion and 5 women with induced abortion were included from attendants of Gynecology department at Al-Kadhimiya Teaching Hospital in Baghdad. In-situ hybridization (ISH) tests were done to detect the level of expression of Fas and FasL in trophoblastic tissue.ResultsThe highest percentage of expression of Fas and FasL was found in the trophoblastic tissue of females with spontaneous abortion (34.36% for Fas and 31.86% for FasL), while the expression of them was low in induced abortion group (9.2% and 9.4%, respectively).ConclusionThe data strengthen the possibility that the apoptosis of trophoblastic cells via enhanced expression of Fas-FasL system is an important mechanism of spontaneous abortion.Key wordsMiscarriage, Trophblast, Apoptosis, Fas, FasL, ISH.

Keywords

Miscarriage --- Trophblast --- Apoptosis --- Fas --- FasL --- ISH


Article
(( Prevalence of Soluble Fas Protein in Breast Cancer Patients:correlation with the Clinico-Pathological Parameters))

Authors: Manar Abed AL-Kreim Abed Oun --- Hedef Dhafir El-Yssin --- Nada A.Al-Alwan
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2016 Volume: 15 Issue: 1 Pages: 107-117
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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ABSTRACTBACKGROUND: Breast cancer is the most common cancer among the Iraqi population. Alteration in the expression of Fas and Fas ligand (FasL) compared to normal tissues are reported in the literature.OBJECTIVE:To investigate whether measuring this tumour marker in serum of breast cancer patients before and after treatment might also be useful markers in the diagnosis, screening and monitoring the malignant tumour progression and response to therapy. METHODS: Serum samples were obtained from (28) apparently healthy women (Control Group) with a mean age of 40.9 ± 7.6 years and (60) female patients complaining from primary breast cancer (Patients Group) with a mean age of 48.3 ± 8.9 years. They were divided according to their clinical end point into: Pre-Surgical Group, Post-Surgical Group and post- chemotherapy Group. Serum sFas level was measured using ELISA kits. RESULTS:The mean serum levels of sFas were significantly elevated (P<0.05) in breast cancer patients than controls. There was no significant influence of the studied personal and pathological characteristics upon biomarkers levels in any of the breast cancer subgroups (P > 0.05). sFas level was found an effective test (P < 0.05) in both pre- surgery and post- chemotherapy groups (accuracy is 87% and 90% respectively). At readings ≥ 300 pg/ml in both groups, sensitivity approached 85%. Fas was not found an effective test in the post surgery group (P > 0.05). CONCLUSION: It could be concluded that sFas is useful for monitoring the response of breast cancer patients to surgery and chemotherapy if the effect of systemic inflammatory reactions is excluded.KEY WORDS: soluble fas,post-chemotherapy.


Article
The Profile of Apoptotic Marker sFas Ligand in seminal plasma of Oligozoospermic men

Authors: abah N. Alwachi --- Anam Rasheed AL-Salihi --- Ahmed Hussein Zwamel
Journal: Iraqi Journal of Embryos and Infertility Researches المجلة العراقية لبحوث الأجنة والعقم ISSN: eISSN: 26166984 / pISSN: 22180265 Year: 2014 Volume: 4 Issue: 2 Pages: 19-24
Publisher: Al-Nahrain University جامعة النهرين

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Background:The Fibroblast associated (Fas) system in the testes has been identified as a keyregulator of apoptosis, a process that greatly influences the germ cell population ofthe testes.Objective:This study measures the level of soluble Fas Ligand (sFasL) in the semen ofoligozoospermic men evaluating the association between seminal plasma sFasL andspermatogenesis.Methods:A total 58 oligozoospermic men and 29 normal volunteers were included in thisstudy. semen was evaluated according to World Health Organization 2010 standardparameters. sFasL was measured using ELISA enzyme immunoassay forquantitative determination of sFasL.Results:s FasL level was found significantly higher (P< 0.01) in seminal plasma ofoligozoospermic men,with weak correlation of the level of this marker with the degree of severity ofoligozoospermia.Conclusions:The apoptotic marker, sFasL is a novel marker found in the seminal plasma ofoligozoospermic men. It's level is higher in cases of oligozoospermia regardless ofits severity.Key words:


Article
SERUM sFAS IN NON-HODGKIN'S LYMPHOMA
عامل فاس الذائب في مصل دم المرضى المصابين بأورام أللاهودجكن اللمفاوية

Authors: Huda S.Baqer هدى سلمان باقر --- Abbas H.Abdulsalam عباس هاشم عبد السلام --- Subh S. Al-Mudalal صبح سالم المدلل
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2007 Volume: 5 Issue: 3 Pages: 3-12
Publisher: Al-Nahrain University جامعة النهرين

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Background: Lymphomas are a heterogenous group of malignancies of B or T cells that usually originate in the lymph nodes. They are divided into Hodgkin and non-Hodgkin’s lymphomas. Disruption of the physiological balance between cell proliferation and death is a universal feature of all cancers. There has been an increasing interest in the role of apoptosis in tumorogenesis of lymphoma. On the molecular base apoptosis is caused by activation of the caspases through extrinsic and intrinsic pathways. Extrinsic pathway centers on tumor necrosis factor family, where the ligand will bind to the cell surface receptor and this in turn will induce apoptosis. Fas receptor is a member of the TNF/NGF receptor superfamily. Fas family is constituted of the receptor, ligand and soluble form. Soluble Fas will compete with Fas receptor for binding to ligand, thus interfering with Fas-L-mediated apoptosis.Objective: To measure the level of serum sFas in non-Hodgkin lymphoma (NHL) and to determine the correlations of it with certain clinical and hematological parameters and chemotherapy treatment.Patients and Methods: This study included 30 patients with NHL (19 males and 11 females), of them 14 patients were newly diagnosed along with 30 apparently healthy controls were involved in this study. The patients were interviewed with history taking, clinical examination and aspirating blood sample for estimation of serum sFas concentration using sandwich ELISA kit (from Chemicon). Also serum CRP level, plasma LDH level, hemoglobin concentration, platelets count, WBC count and peripheral blood film were all performed using standard techniques.Results: This study revealed that the serum sFas concentration was significantly higher in NHL patients than in healthy controls (p = 0.0001). This increase was significantly higher in pretreated patients and closely related to the pathological grade of NHL (p = 0.0002 and 0.0035 respectively).Conclusions: Serum sFas is a simple, non-invasive and clinically useful laboratory parameter .It maybe used as an auxiliary marker to assess the prognosis and the therapeutic planning in NHL.Keywords: Fas, NHL.

خلفية الدراسة: الأورام اللمفاوية هي مجموعة غير متجانسة من أورام خلايا باء أو تاء والتي عادة تنشأ في العقد اللمفاوية .وهي تقسم الى أورام هودجكن و اللاهودجكن اللمفاوية . إن تمزيق التوازن الفسيولوجي ما بين تكاثر الخلايا و موتها هو صفة لكل الأورام الخبيثة . لقد كان هنالك اهتمام متزايد بدور موت الخلايا المبرمج في تكوين الورم اللمفاوي. بناء على الأساس الجزيئي إن موت لاخلايا المبرمج هو بسبب تنشيط إنزيم الكاسبيز من خلال الطرق الخارجية و الداخلية .الطريق الخارجي يركز على عائلة عامل تنخر الورم، والتي يرتبط فيها الرابط إلى مستقبل سطح الخلية وهذا بدوره سوف يحدث موت الخلايا المبرمج. إن مستقبل فاس هو أحد أعضاء العائلة العليا لمستقبلات عامل تنخر الورم/ عامل نمو العصب. عائلة فاس تتكون من المستقبل والرابط والشكل الذائب . فاس الذائب سوف يتنافس مع مستقبل فاس للارتباط مع الرابط ، وبذلك سوف يتداخل مع موت الخلايا المبرمج الذي يتوسطه فاس الرابط .هدف الدراسة : لقياس مستوى فاس الذائب في مصل دم مرضى أورام أللاهودجكن اللمفية . بالإضافة إلى تقرير العلاقة بين تركيز فاس الذائب في مصل الدم ومؤشرات سريريه محددة، ومؤشرات الدم ، والعلاج الكيماوي لورم اللاهودجكن .المرضى والطرق: شملت الدراسة 30 مريضا" مصابا" بورم اللاهودجكن بضمنهم19 ذكرا" و11 أنثى . من هؤلاء المرضى 14 مريضا"شخصوا حديثا" . بالإضافة هناك 30 شخص سليم استخدموا كمجموعة ضبطية . لقد قوبل المرضى وأخذ منهم تاريخهم المرضي وفحصوا سريريا" وسحبت منهم ومن العينة الضبطية عينة دم لتقدير تركيز فاس الذائب في مصل الدم باستعمال عدة شطيرة ألألايزا من شركة كيميكون. وكذلك مستوى بروتين سى التفاعلي في مصل الدم ومستوى الخميرة المخسفجة للاكتات في بلازما الدم وتركيز الهيموغلوبين وعدد الصفيحات الدموية وعدد خلايا الدم البيضاء وفلم الدم المحيطي وقد نفذت جميعها باستخدام التقنيات المعيارية . النتائج : هذه الدراسة كشفت إن تركيز فاس الذائب كان مرتفعا" بدرجة ذات أهمية إحصائية عند مرضى أورام أللاهودجكن اللمفية مقارنة بالمجموعة الضبطية (0.001) وهذه الزيادة كانت أعلى بدرجة ذات أهمية إحصائية عند المرضى قبل أخذ العلاج ومتعلقة بصورة وطيدة بالدرجة الباثولوجية لورم أللاهودجكن أللمفاوي (0.0002 و0.0035 على التوالي).ألاستنتاج :إن فاس الذائب هو فحص بسيط ، غير إجتياجى وموفر للوقت . ومن الممكن أن يستعمل كمؤشر مختبري مفيد سريريا" عند مرضى أورام أللاهودجكن أللمفية . إن فاس الذائب ممكن يستعمل كمؤشر مساعد لتقيم شدًة الورم والتكهن بمسير المرض وكذلك التخطيط لخطة العلاج عند مرضى أورام أللاهودجكن أللمفية .مفتاح الكلمات : فاس ، اللاهودجكن


Article
Molecular and Immunopathological Role of Nuclear Factor K B Detected By Insitu Hybridization in Pathogenesis of Chronic Atrophic Gastritis in Iraqi Patients

Author: Ali Ibrahim Ali
Journal: Diyala Journal For Pure Science مجلة ديالى للعلوم الصرفة ISSN: 83732222 25189255 Year: 2010 Volume: 6 Issue: 3 Pages: 187-214
Publisher: Diyala University جامعة ديالى

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Abstract

Chronic Atrophic gastritis (CAG) is a histopathological entity characterized by chronic inflammation of the gastric mucosa with loss of gastric glandular cells and replacement by intestinal-type epithelium and fibrous tissue. Atrophy of the gastric mucosa is the endpoint of chronic processes, such as chronic gastritis associated with Helicobacter pylori infection. This study designed to determine the molecular role of NFkB signaling on Fas receptor mediated apoptosis in H.pylori associated CAG cases. Forty five patients suffering from CAG that proved via endoscopical and histopathological examination were eligible for this study. Males represent (73.33%) and the rest (26.67%) were females with a mean age of 39.26 years .twenty apparently healthy volunteers (10 male and 10 female) with the mean age 38 years and age range (23-58) years were enrolled as control group. Multiple mucosal biopsy specimens were taken from the inflamed area of gastric mucosa for rapid urease test other biopsy specimens were fixed with 10% buffered formalin for preparation of paraffin embedded tissue blocks to prepare slides for histological examination using haematoxylin-eosin stain to evaluate the grades of Polymorphonuclear leukocytes(PMNs) and lymphocytes infiltration . Insitu hybridization technique (ISH) using biotinylated long DNA probe for human NF-KB Gene and for human FAS gene as well as H.pylori Cag A gene were used for detection of NF-KB and Fas mRNA and Cag A mRNA expression in tissue infiltrated lymphocytes and neutrophils as well as gastric epithelial cells and for detection of H.pylori Cag A. This study explained that there was a statistical significant positive linear relationship between lymphocytes grade and Fas mRNA expression in PMNs and gastric epithelial cells. PMN grade has significant positive linear relationship with each of the following markers: Tissue Fas mRNA expression. Tissue NFkB mRNA expression, lymphocytes NFkB mRNA expression, PMN NFkB mRNA expression, Cag A mRNA expression. Lymphocytes Fas mRNA expression has significant positive linear relationship with PMN Fas mRNA expression and Cag A mRNA expression .PMN Fas mRNA expression has significant positive linear relationship with Cag A mRNA expression and negative linear relationship with tissue NFkB mRNA expression . Tissue Fas mRNA expression has significant positive linear relationship with tissue NFkB mRNA expression ; lymphocytes NFkB mRNA expression ; PMN NFkB mRNA expression ;Cag A mRNA expression . Tissue NFKB mRNA expression has significant positive linear relationship with lymphocytes NFkB mRNA expression; PMN NFkB mRNA expression. Lymphocytes NFkB mRNA expression has significant positive linear relationship with PMN NFkB mRNA expression. This study conclude that NFkB play a vital role in determination of immunopathological and inflammatory events of gastritis that leads finally to development of atrophic changes


Article
Effect of dietary supplementation of crushed seed of Coriander Coriandrum sativum on Some Physiological & Immunological parameters of broiler Hubbard flex.
تأثير الإضافة الغذائيـــة لمسحوق بذور الكزبرة Coriandrum sativum في بعض المعايير الفسلجية والمناعية لفروج اللحم (هبرد فلكسHubbard flex ).

Author: سرى صافي عبيس
Journal: journal of kerbala university مجلة جامعة كربلاء ISSN: 18130410 Year: 2013 Volume: 11 Issue: 3 Pages: 194-201
Publisher: Kerbala University جامعة كربلاء

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Abstract

The present study has been aimed to determine the positive effect that can be detected by crushed seed of Coriander Coriandrum sativum addition to the broilers diet on some physiological & immunological parameters. One hundred and fifty broiler chicks of one day age, were randomly divided into three equal groups. First group (control) fed on the standard provender along the experimental period (42 days), second group (T1) fed on standard provender supplemented with 2.5%, third group (T2) fed on standard provender supplemented with 5%. At the end of experiment, body weight was recorded and blood samples were obtained from wing vein for estimation of white blood cells counts, differential leukocytes counts, Phagocytosis activity for neutrophil and monocytes. Total protein, globulin, glucose, total cholesterol, IgG, IgA & IgM concentrations in blood serum were calculated. Then, birds were sacrificed and the liver, kidneys and spleen obtained for histological measurement. The result revealed that crushed seed of Coriander Coriandrum sativum supplementation in the two treated groups caused a significant increase (P<0.05) in body weight & weight of liver when compared with control group. The result revealed that the second and third groups were significantly (P<0.05) recorded higher values in their white blood cells counts, Lymphocytes, Monocytes counts & Phagocytosis activity. At the same time, the result of total protein & globulin showed a significant increase (P<0.05) in two treated groups compared with control. On the other hand, serum concentration of glucose and cholesterol showed a significant decrease (P<0.05) in the two treated groups compared with control. At the same time, the result of IgG & IgA concentrations, revealed a significant increase (P<0.05) in IgG & IgA concentrations in the both T1 and T2 groups compared with control.

هدفت الدراسة الحالية لمعرفة الآثار الايجابية التي قد تنتج عن الإضافة الغذائية لمسحوق بذور الكزبرة Coriandrum sativum إلى علائق فروج اللحم في بعض الجوانب الفسلجية المناعية. قسم 150 فرخاً من فروج اللحم بعمر يوم واحد إلى ثلاث معاملات بواقع 50 صوص لكل مجموعة : (C ) مثلت مجموعة السيطرة تناولت العليقة الأساسية خالية من أي إضافة. (T1 ) مثلت مجموعة المعاملة الأولى تناولت العليقة الأساسية مضافاً إليها مسحوق بذور الكزبرة بقيمة 2.5% ،(T2) مثلت مجموعة المعاملة الثانية تناولت العليقة الأساسية مضافاً إليها مسحوق بذور الكزبرة بقيمة 5%. واستمرت التجربة لمدة 42 يوماً، وفي نهاية مدة التجربة، تم قياس الأوزان النهائية للحيوانات، ثم أخذت نماذج دم من الوريد الجناحي لغرض قياس المعايير الدمية (العدد الكلي والتفريقي لخلايا الدم البيض)، فضلاً على اختبار كفاءة عملية البلعمة للخلايا العدلة والوحيدة وبعدها تم الحصول على مصل الدم وقياس مستوى البروتين والكلوبيولين والكلوكوز والكولسترول وIgG, IgA, IgM ، كما تم استئصال كل من الكبد والكليتين والطحال لغرض حساب أوزانها النسبية. أظهرت نتائج الدراسة الحالية ارتفاع معنوي (P<0.05) في معدل وزن الجسم النهائي وفي نسبة وزن الكبد في مجموعتي المعاملة مقارنةً مع مجموعة السيطرة، كما أشارت نتائج الدراسة الحالية إلى وجود إرتفاع معنوي (P<0.05) في العدد الكلي لخلايا الدم البيض ونسبة الخلايا اللمفية والوحيدة ومعدل الخلايا البلعمية في مجموعتي المعاملة مقارنةً بمجموعة السيطرة في نهاية التجربة. كما أشارت نتائج الدراسة إلى إن الإضافة الغذائية لمسحوق البذور (في المجموعتين T2 وT1) أدت إلى حصول إرتفاع معنوي (P<0.05) في تركيز البروتين الكلي والكلوبيولين في مجموعتي المعاملة مقارنةً بمجموعة السيطرة، كما أظهرت النتائج انخفاض معنوي (P<0.05) في تركيز سكر الكلوكوز والكولسترول في مجموعتي المعاملة بالمقارنة مع مجموعة السيطرة. من جانب آخر، أشارت النتائج إلى حدوث ارتفاع معنوي (P<0.05) في مستوى تركيز IgG, IgA في مصل الدم في مجموعتي المعاملة مقارنةً مع مجموعة السيطرة.

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