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Article
Hepatoprotective Effect of the Aqueous Extract of Camellia sinensis Against Methotrexate-induced Liver Damage in Rats

Author: Ahmed H. Jwied
Journal: Iraqi Journal of Pharmaceutical Sciences المجلة العراقية للعلوم الصيدلانية ISSN: 16833597 Year: 2009 Volume: 18 Issue: 2 Pages: 73-79
Publisher: Baghdad University جامعة بغداد

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Abstract

Methotrexate (MTX) is a folate antagonist widely used in the treatment of neoplastic diseases; its biotransformation in the liver produced active metabolites that promote hepatotoxicity. The present study was designed to evaluate the hepatoprotective effect of aqueous extract of Camellia sinensis (Green tea) against MTX-induced liver damage in rats. A model of liver injury in rats was induced by intraperitoneal injection of 20mg/kg MTX as a single dose followed by saline and 1.25% and 2.5% aqueous extract of green tea (GTE) were orally administered 7 days prior and 5 days after MTX-intoxication as a sole source of drinking water. After killing the animals, blood samples were obtained for evaluation of serum levels of alanine and aspartate aminotransferases (ALT and AST) and alkaline phosphatase (ALP) activities, while liver tissue homogenate was prepared to evaluate tissue levels of glutathione (GSH) and malondialdehyde (MDA). Additionally, liver tissue sections were prepared and stained with hematoxylin and eiosin for histological evaluation. The results showed that administration of green tea extract (GTE) significantly decreased the elevated levels of ALT, AST and ALP activities in the serum compared to MTX-treated group. Treatment of animals with GTE 7 days before and 5 days after MTX also elevates GSH levels and decreases MDA levels significantly compared to MTX-treated group, this was associated with improving histological features that already impaired due to exposure to MTX. In conclusion, treatment of rats with GTE protects hepatic tissue against MTX-induced liver damage in dose dependent manner.

مثوتركزيت هو نظير للفوليت ذو استعمال واسع في علاج الامراض السرطانية, يتحول الميثوتركزيت بعد ايضه داخل الكبد الى ناتج سام يؤدي الى تسمم الكبد.صممت هذه الدراسة لتقييم فعالية جرع مختلفة من المستخلص المائي للشاي الاخضر(Camellia sinensis) في حماية كبد الجرذان المختبرية من المثوتركزيت.تم استحداث التسمم الكبدي للجرذ بواسطة حقنه داخل الغشاء البريتوني ب (٢٠ ملغم /كلغم )من مادة الميثوتركزيت لمدة خمسة ايام متتالية وذلك يتم بالتتابع مع معالجة الجرذ ب(٢٥,١% -٥,٢%) من مستخلص المائي الشاي الاخضر عن طريق الفم كبديل عن ماء الشرب لمدة خمسة ايام ماقبل و سبعة ايام ما بعد الحقن بالميثوتركزيت.بعد قتل الحيوان اخذت عينة من مصل الدم لقياس مستوى فعالية الالانين( ( alanineو الاسبارتيت امينوترانسفيريز (aspartate aminotransferases) , من جهه اخرى ناخذ مستخلص انسجة الكبد و نقيِِِِِِِم الكمية النسيجية من الجلوتاثايون ( (glutathione و المالوندايالدهايد ( (malondialdehyde والالكلاين فوسفتيز (alkaline phosphatase) بالاضافة الى اخذ جزء من نسيج الكبد للفحص النسيجي بواسطة صبغه بمواد الايوسين و الهيماتوكسلين.اظهر تحليل النتائج ان اعطاء مستخلص الشاي الاخضر يقلل و بشكل ملحوظ مستويات فعالية كل من الالانين , الاسبارتيت امينوترانسفيريز و الالكالاين فوسفتيز( alkaline phosphatase) وذلك بالمقارنة مع الجرذان غير المعالجة بالشاي الاخضر و التي تم حقنها بالميثوتركزيت وادى ايضا الى زيادة مستوى الجلوتاثايون وخفض مستوى المالونديالدهايد بصورة ملحوظة بالمقارنة مع الحيوانات التي اعطيت الميثوتركسيت و كان هذا واضحا من تحسن الشكل النسيجي للكبد و الذي كان متضررا من مادة الميثوتركزيت .كاستنتاج ,المعالجةالمبكرة بالجرع المعتمدة من الشاي الاخضر تحمي انسجة الكبد من الضرر الناتج عن الميثوتركزيت.

Keywords

Green tea --- Hepatotoxicity


Article
Extraction of Catechin from green tea plants Camellia sinensis
استخلاص الكاتشين من الشاي الاخضر Camellia sinensis

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Abstract

Catechin was extracted from green tea Camellia sinensis by using liquid-liquid chromatography. Extraction was done using hot water and then fractionation of the extract in two-stages first mixed with chloroform; the aqueous layer was taken and the second step mixed with ethyl acetate to remove all the apolar compounds and dark brown colour substance was obtained nonviscous and this was supposed to be catechin compound. Catechin has been diagnosed by using chemical reagent; thin layer chromatography (TLC) and the relative front (Rf) was 0.92 when using the mobile phase acetic acid: chloroform (9:1). The melting point has reached 120oC. The catechin was conducted by the use of high-performance liquid chromatography (HPLC) and was diagnosed by FTIR spectrum.

تم استخلاص الكاتشين (Catechin) من الشاي الاخضر Camellia sinensis بأستخدام طريقة الفصل بالتجزئة ، اذ تم الاستخلاص اولاً بالماء الحار ثم تجزئة المستخلص على مرحلتين الاولى خلطه مع الكلورفوم وبعدها تم الحصول عل الطبقة المائية ، ثم المرحلة الثانية خلطه مع خلات الاثيل للتخلص من المركبات الغير قطبية وقد تم الحصول على مادة بنية اللون غامقة تخلو من اللزوجة وهي مادة الكاتشين وقد تم تشخيصها والتاكد منها باستخدام الكواشف الكيمائية وكذلك تقنية الطبقة الرقيقة (TLC) وكانت مقدار الحركة النسبية للمركب 0.92 عند استخدام محلول الفصل حامض الخليك: كلورفورم (9:1) اما درجة الانصهار فقد بلغت 120م وقد تم تشخيص المركب باستخدام تقنية كروموتوغرافيا السائل الكفاية (HPLC) إذ ظهرت حزمة واحدة ، وكذلك بواسطة تقنية FTIR.

Keywords

Green tea --- Catechin --- HPLC --- TLC


Article
IN VITRO AND IN VIVO STUDY OF GREEN AND BLACK TEA ANTIMICROBIAL ACTIVITY ON METHICILLIN RESISTANT STAPHYLOCOCUS AUREUS
دراسة الفعالية الضد جرثومية للشاي الأخضر و الأسود على جرثومة methicillin resistant Staphylococcus aureus (MRSA) داخل وخارج الجسم الحي.

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Abstract

The in vitro study was compared the antibacterial activity of green tea andblack tea extracts against methicillin resistant Staphylococcus aureus (MRSA). Greentea extract was found to have higher antimicrobial activity on MRSA with aninhibition zone of 20 mm at a concentration of 10 gm/100 ml. For black tea extract,the inhibition zone was 15 mm at same concentration .The minimum inhibitoryconcentrations for the green tea and black tea were 4mg/ml and 8mg/ml respectively.The in vivo study of the antimicrobial effect of both green and black tea wasinvestigated, by subcutaneous inoculation of four rabbits with MRSA bacteria. Afterthat immediately treated with a series dilution of tested material (green and black tea),the development of swelling and the degree of necrosis were scored. The green teashowed marked reduction in the size of swelling less than 2mm at the concentration of10mg/ ml; also there is a marked reduction in the severity of necrosis. For black tea amarked reduction occur in the size of swelling (6-10mm) at the concentration of10mg/100ml; also there is a marked reduction in the severity of necrosis at the sameconcentration. This study concludes that green and black tea extracts were showed tohave an antibacterial activity against MRSA and a therapeutic effect against skininfection caused by MRSA in rabbits

أجريت الدراسة المختبرية لمقارنة التأثير التثبيطي لكل من الشاي الأخضر و الأسود ضد البكتيريا المرضية العنقودية المقاومة للمثيسلين ,أظهرت الدراسة إن خلاصة الشاي الأخضر لها تأثير تثبيطي على الجرثومة methicillin resistant Staphylococcus aureus (MRSA) في الوسط الزرعي حيث كانت منطقة التثبيط 20)) ملم عند التركيز 10)غم /100 مل ) بينما كانت منطقة التثبيط للشاي الأسود 15) ) ملم عند التركيز ذاته. وجد ان اقل تركيز مثبط للشاي الأخضر و الأسود ( 4 ) مايكرو غرام / مل و( 8 )مايكرو غرام / مل على التوالي .ولقد أثبتت الدراسة داخل الجسم التاثير المضاد للجراثيم لكل من الشاي الاخضر و الشاي الاسود, وذلك من خلال حقن اربعة أرانب تحت الجلد بالبكتيريا المرضية MRSA, وبعد ذلك تمت عملية حقن المنطقه مباشرة بتخافيف متعدده من عينات الشاي الاخضر و الشاي الاسود, لقد تمت عملية قياس درجة الالتهاب و التورم الحاصله في منطقه الحقن. اظهرت الدراسه ان للشاي الأخضر تأثير واضح في انخفاض حجم التورم الحاصل في المنطقة الى اقل من 2ملم في التركيز المساوي ل 10ملغرام/100مل, وتأثير واضح في انخفاض درجة التقرح الحاصله في المنطقه. كذلك اظهرت الدراسه ان للشاي الاسود تأثير واضح في انخفاض حجم التورم الحاصل في المنطقة بين 6-10 ملم في التركيز المساوي ل 10ملغرام/100مل وكذلك تأثير واضح في انخفاض درجة التقرح الحاصل في المنطقه و في نفس التركيز. لقد اثبتت الدراسه ان للشاي الاخضر و الشاي الاسود تاثير علاجي للامراض الجلدية في الارانب الحاصلة بسبب الاصابة MRSA


Article
Role of Green Tea Extract in Reducing Pathological Changes of Induced Diabetes Mellitus in Rats
دور مستخلص الشاي الأخضر في خفض التغييرات المرضية لداء السكري المستحدث في الجرذان

Authors: Kamal A. Khidir كمال احمد خضر --- Sarmad R. Kareem سرمد رحيم كريم --- Ismail S. Kakey اسماعيل صالح الكاكي
Journal: Rafidain journal of science مجلة علوم الرافدين ISSN: 16089391 Year: 2013 Volume: 24 Issue: 4 E Pages: 1-12
Publisher: Mosul University جامعة الموصل

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Thirty male albino rats were used to investigate the effect of green tea extract on pancreas, renal and hepatic histological parameters in alloxan induced diabetes mellitus.The animals were randomly distributed into five groups of six animals in each group. The first group was regarded as normal healthy control, the second group was regarded as diabetic control, the third group was treated with insulin, the fourth group was treated with green tea extract, 50 mg/kg body weight (GTE50), and the fifth group was treated with GTE100.This study showed that functionally diabetes related organs such as pancreas, kidney and liver showed diabetes related pathological changes and these revealed a noticeable tendency for reversion to normal after treatment with green tea extract.

في هذه الدراسة تم استخدام ثلاثين من ذكور الجرذان البيض للتحري عن تأثير مستخلص الشاي الأخضر على التغييرات النسجية في البنكرياس والكلية و الكبد في الجرذان المصابة بداء السكري المستحدث بالالوكسان.تم توزيع الحيوانات عشوائيا الى خمس مجاميع بمعدل ست جرذان في كل مجموعة. ثم عدت المجموعة الأولى مجموعة قياس سليمة والمجموعة الثانية مجموعة قياس مصابة بالسكري والمجموعة الثالثة عوملت بالانسولين والمجموعة الرابعة عوملت بمستخلص الشاي الأخضر بتركيز 50 ملغم/كغم من وزن الجسم والمجموعة الخامسة عوملت بمستخلص الشاي الأخضر بتركيز 100 ملغم/كغم من وزن الجسم.أظهرت الدراسة أن الأعضاء المتأثرة بداء السكري وهي البنكرياس والكلية والكبد قد أظهرت تغييرات مرضية نسجية وان هذه التغييرات أظهرت تحسنا واضحا بعد المعاملة بمستخلص الشاي الأخضر.

Keywords

rat --- green tea --- alloxan --- diabetes


Article
Micronucleus formation assay and phagocytic index in mice administered water leaf extracts of Camellia sinensis

Authors: Bushra H. Saleh بشرى صالح --- Rukaya M. Ebraheem رقية محمد ابراهيم --- Shahlaa M. Salih شهلاء صالح
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2010 Volume: 52 Issue: 3 Pages: 355-358
Publisher: Baghdad University جامعة بغداد

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Background: Green tea Camellia sinensis is non fermented tea. The tea is an infusion of flavorful leaves that has been consumed for centuries as a beverage and is valued for its medicinal properties. Over the last few decades green tea has been subjected to many scientific and medical studies to determine the extent of its long-purported health benefits, with some evidence suggesting regular green tea drinkers may have lower chances of heart disease and developing certain types of cancer. Green tea has also been claimed useful for weight loss management.Methods: Three doses of the hot water extract were used (19.2, 38.4 and 57.6 mg/kg) to investigate micronucleus formation and phagocytic index. Mice were divided into two groups. Group one; control and group two; mice were administered with three doses of the green tea extract orally as a single dose (0.1 ml) per day for 7 days. Then the mice were sacrificed in day 8 for immunological and cytogenetic assessments.Results: Results revealed that the first and third doses of green tea hot water extract were significantly effective in enhancing the values of phagocytic index and reducing micronucleus formation.Conclusions: Hot water may be a good solvent to extract active component from Camellia sinensis especially catechin, polyphenols, polysaccharides, flavonoids and vitamins. The high dose of hot water extracts of Camellia sinensis showed an excellent enhancing effects on the function of immune system of mice, moreover, reduction in micronucleus formation was recorded at such dose.Key words: Green tea, Cytogenetic, Immunological effect


Article
The antibacterial effect of herbal alternative, green tea and Salvadora Persica (Siwak) extracts on Entercoccus faecalis

Author: Abdulkareem J. Al-Azzawi عبد الكريم جاسم العزاوي
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2015 Volume: 27 Issue: 2 Pages: 1-5
Publisher: Baghdad University جامعة بغداد

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Background: Disinfection and shaping of the canal with a combination of chemical agents and endodonticinstruments play an important role in the success of endodontic therapy. Eliminating the microorganisms within thepulp space is a critical and important objective in treating a tooth with apical periodontitis. This study wasconducted to evaluate the antibacterial properties of herbal alternatives (Green tea and siwak extracts) as possibleirrigants during endodontic treatment compared with the conventional irrigation solutions.Materials and methods: Salvadora Persica (siwak) and Green Tea solutions were prepared.An agar diffusion test wasperformed on Mueller-Hinton agar using the well diffusion method. The tested solutions (5.25% NaOCl, 2%Chlorhexidine gluconate, 5% Siwak extract and 5% Green tea extract)were used to fill the wells that were made inthe agar media respectively.Plates were left to incubate for 24 hr. at 37°C. Zones of inhibition of the bacterial growthwere calculated to measure the antibacterial effect of the tested irrigants.Results: Sodium hypochlorite had the highest mean value (29.88) followed by Chlorhexidine which had a mean valueof (26.13), Siwak with mean value of (11.25) and Green tea being the least with mean value of (8.88). ANOVA testshowed a highly statistical difference with a P-value of (0.000).Conclusions: NaOCl still the superior irrigant than other irrigants. Herbal alternatives (Siwak and Green tea) can beused as possible irrigants solution to disinfect the root canal system from Enterococcus faecalis during endodontictreatments.


Article
Antibacterial effects of green tea extracts on Aggregatibacter actinomycetemcomitans (In-Vitro study)

Authors: Mays Jamal Mageed ميس جمال مجيد --- Saif Sehaam Saliem سيف سهام سليم
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2015 Volume: 27 Issue: 3 Pages: 102-108
Publisher: Baghdad University جامعة بغداد

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Background: Green Tea is made from the leaf of the plant “Camellia sinensis”. Green tea is reported to containthousands of bioactive ingredients including catechins which have shown great promise for having antimicrobialeffects. Periodontal diseases represent one of the most prevalent diseases around the world and the main etiologicfactor behind it, is plaque accumulation, in addition certain kinds of bacteria have been detected frequently insubjects suffering from periodontitis, Several studies suggested that the outcome of periodontal treatment is better ifparticular pathogens including Aggregatibacteractinomycetemcomitans can no longer be detected after therapy.Materials and Methods: plaque samples were collected from 20 patients suffering from chronic periodontitis withprobing pocket depth of at least 6 mm, Aggregatibacteractinomycetemcomitans (A.A) was isolated anddiagnosed according to morphological characteristics and biochemical tests. Green tea leaves were extracted byusing water and alcohol. The first experiment involved testing the sensitivity of A.A to different concentrations of theextracts using agar well diffusion method,the second experiment involved determination of the minimum inhibitoryconcentration and then determination of the minimum bactericidal concentration of the extract against thebacteria, laboratory analysis of green tea extracts using high pressure liquid chromatography (HPLC) wasperformed.Results: Both green tea extracts were effective in inhibition of Aggregatibacteractinomycetemcomitans using agarwell diffusion method, 90% and 100% concentrations of alcoholic extract showed larger inhibition zones thanchlorhexidinegluconate 0.2% with statistically significant difference, CHX showed higher inhibition zones than allaqueous extract concentrations.The MIC (minimum inhibitory concentration) of alcoholic green tea extract thatinhibit Aggregatibacteractinomycetemcomitans growth was 60%, The MIC of aqueous green tea extract thatinhibits Aggregatibacteractinomycetemcomitans growth was 70%.The MBC (minimum bactericidal concentration)of alcoholic green tea extract that killsAggregatibacteractinomycetemcomitans was 80%, the MBC of aqueousgreen tea extract that kills Aggregatibacteractinomycetemcomitans growth was 90%.HPLC analysis of aqueous and alcoholic green tea extracts revealed that alcoholic extract contained higherconcentration of EGCG while aqueous extract had higher content of catechin and epicatechin.Conclusion: Green tea extracts were effective against Aggregatibacteractinomycetemcomitans, alcoholic greentea extract showed inhibition ability more than the aqueous green tea extract and more than CHX and it showedbactericidal activity at 80%,90% and 100% concentrations


Article
Cytotoxic effect of green tea leaf extract on tumor cell line
التأثير السمي لمستخلص أوراق الشاي الاخضر على الخلايا السرطانية

Author: Manhal F. Ahmed منهل فاروق أحمد
Journal: The Iraqi Journal of Veterinary Medicine المجلة الطبية البيطرية العراقية ISSN: 16095693 Year: 2017 Volume: 41 Issue: 1 Pages: 71-75
Publisher: Baghdad University جامعة بغداد

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The study was conducted to evaluate antitumor effects of green tea (Camellia sinensis) extracts (aqueous and methanolic) on Rhabdomyosarcoma; cell line and a normal cell line; mouse embryo fibroblast; Chemical detections of green tea extracts revealed that the aqueous and methanolic extracts were positive for flavonoids, alkaloids, phenol and glycosides. The percentage growth inhibition of five plant concentrations (50, 100, 250, 500 and 1000 µg/ml) were assessed in vitro using tumor cell lines Rhabdomyosarcoma and normal cell line mouse embryonic fibroblasts. The results revealed that the five concentrations of the plant extracts showed anti-tumor properties in a concentration-dependent manner, and the methanolic extract recorded better values of percentage growth inhibition than the aqueous extract in Rhabdomyosarcoma cell lines, while, less percentage growth inhibition values were recorded in the mouse embryo fibroblast cell line.

صممت الدراسة لتقييم التأثير السمي للمستخلص الكحولي والمائي لأوراق الشاي الاخضرعلى خط خلايا سرطان العضلات المخططة البشري وخط خلايا الفأرالجنينية الطبيعية المولدة للألياف. واظهرت الفحوصات الكيميائية للمستخلصين المائي والكحولي للنبات وجود مركبات الفلافونويد، القلويدات، الفينول والجليكوسيدات. وقيمت نسبة تثبيط النمو لخمسة تراكيز (50 و 100 و250 و500 و1000 ميكروغرام/ مل) للمستخلصين الكحولي والمائي للنبات في المختبر باستخدام خط الخلايا السرطانية والخط الخلايا الطبيعية وكشفت النتائج أن التراكيز الخمسة للمستخلصين الكحولي والمائي للنبات أظهرت خصائص مضادة للورم بطريقة تعتمد على التركيز، وسجل المستخلص الكحولي للنبات قيم تثبيط للنمو أفضل من المستخلص المائي في خط خلايا سرطان العضلات المخططة البشري، بينما سجلت أقل قيم لتثبيط النمو في خط خلايا الفأر الجنينية الطبيعية المولدة للألياف.


Article
The Effect of Green Tea Polyphenol (EGCG) on Orthodontic Micro-Implant Stability: An experimental study

Authors: Mustafa Muath Hamed Al-Sultan --- Zaid Salim Twafeeq
Journal: Journal of Oral and Dental Research مجلة طب الفم والاسنان ISSN: 23106417 Year: 2017 Volume: 4 Issue: 2 Pages: 102-109
Publisher: Iraqi Association for Oral Research الجمعية العراقية لبحوث طب الفم

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Background Increasing the orthodontic micro-implant stability and enhancing bone response around it, is a fundamental objective of successful therapy. Materials and Methods Thirty orthodontic micro-implants, 1.3 mm in width and 5 mm in length (Dentos, AbsoAnchor, South Korea), were used with three implants were inserted in the right tibias of ten New Zealand rabbits, the rabbits categories into two groups, fve each. The primary stability was measured once the implant inserted, using Periotest. The experimental group fed (0.5% wt/vol/day) Green Tea, using the loading technique, for four weeks interval. At the end of the experimental period, the rabbits were sacrifced, and the secondary stability measurement was taken. Results Using paired t-test, the results showed a signifcant increase in secondary stability for the experimental group after four weeks experimental period when compared with the primary one. While the control group showed a non-signifcant increase in the secondary stability compared with the primary one. Conclusion This study concluded that the Green Tea could enhance bone response around orthodontic micro-implant within four weeks healing period and consequently increase the stability of orthodontic micro-implant

Keywords

Green Tea --- Micro-Implant --- Stability


Article
STUDY THE EFFECT OF PURIFIED CATECHINS OF GREEN TEA ON THE MOST IMPORTANT CAUSES OF BACTERIAL INFECTIONS OF THE URINARY TRACT AND SYNERGISTIC EFFECT
دراسة تأثير الكاتشين المنقى من الشاي الاخضر في أهم المسببات البكتيرية لالتهابات المجاري البولية والتأثير التآزري عليها

Authors: ESSAM F.A.Al-jumaily عصام فاضل الجميلي --- Yasir A.J.Al-Abdli --- Nazar E.Nasser ياسر عادل جبار --- نزار ادور ناصر
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2011 Volume: 52 Issue: 3 Pages: 324-328
Publisher: Baghdad University جامعة بغداد

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Abstract

Two hundred urine samples were collected from patients infected with acute and chronic urinary tract infection, 85 bacterial isolate were isolated from these samples and were diagnosed using biochemical tests and kit API test, most of them identified E.coli (23.5%) K.pneumoniae rate (17.6%). Isolates were selected E.coli and K.pneumoniae according to their isolation rate from urinary tract infection cases and their antibiotic resistance. The inhibition effect of the purified catechin from the green tea was studied when the isolates were grown in the following concentrations (25, 50,75, 100) mg/ml. The concentration of 100 mg/ml showed a high action against the two species. The combination effect of the purified catechin was estimated with both Amikacin and Gentamycin on the resistant isolates to these antibiotics (E.coli and K.pneumoniae) and has been compared to the minimum inhibitor concentration of the isolates before and after the confusion of antioxidants, as the effect of mixing Amikacin and Gentamycin with Catechin, it gave a synergic effect with Amikacin on two isolates, either Gentamycin gave synergic effect on K.pneumoniae.

جمعت 200 عينة إدرار من مرضى مصابين بالتهاب المجاري البولية الحاد والمزمن, عُزلت 85 عزلة بكتيرية منها, وشخصت بأستخدام الفحوصات الكيموحيوية واختبار العدة API, ,وكان معظمها يعود الى E.coli بنسبة(23.5%) وK.pneumoniae بنسبة(17.6%). أختيرت العزلات البكتيرية E.coli وK.pneumoniae على أساس ترددها الأكثر في إصابات المجاري البولية ومقاومتها لتأثير المضادات الحيوية. درس التأثير المثبط للكاتشين Catechin المنقى من الشاي الأخضر في نمو العزلات عند التراكيز (25, 50100 ,75 ,) ملغم/مليلتر, وقد أظهر التركيز(100) ملغم/ مليلتر فعالية ضد كلا الجنسين من البكتريا. قدر التأثيرالخلطي المركب الكاتشين المنقى Catechin)) مع المضادينAmikacin وGentamycin على العزلات البكتيرية المقاومة للمضادين وهما E.coliوK.pneumoniae وقد تمت مقارنة التركيز المثبط الادنى للعزلات قبل وبعد الخلط للمضادات, اذ كان تأثير خلط Amikacin مع الكاتشين (Catechin) تآزرياً مع مضاد Amikacin على العزلتين , اما مضاد Gentamycin فقد اعطى تأثيراَُ تآزرياعلى بكتيريا K.pneumoniae.

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