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Article
HLA Typing in Iraqi Patients with Obesity and Primary Osteoarthritis.

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Abstract

Background: Osteoarthritis (OA) is a degenerative joint disease. It is one of the major causes of disability in developed and developing countries. Human leukocyte antigen (HLA) as part of immune system has a role in the disease process.Objectives: To investigate whether there is an association between HLA class II-DRB and OA.Methods: A case control study with 26 patients with osteoarthritis and 22 apparently healthy obese control persons matching in ethnicity were enrolled in this study during the period between October 2012 till March 2013. Direct interview was done with each patient and HLA typing was done by molecular method using Sequence Specific Primer (PCR-SSP) method using One Lambda Kit-USA. Results: The results showed that females were more affected than males with disease when compared with control. Odds ratio were used to test level of significance. This study showed that HLA DR4 (DRB1*04), DR2 (DRB1*15 and DRB1*16), DR9 (DRB1*09), DR10 (DRB1*10, DRB5*, DRB4* and DRB3*) (odds ratio: 14.26, 9, 9, 9, 14.26, 9.5 and 4.5) respectively are associated with OA.Conclusions: OA is highly associated with HLA class II DR4 (DRB1*04), DR2 (DRB1*15, DRB1*16), DR9 (DRB1*09), and DR10 (DRB1*10).DR5 (DRB1*05) is not associated with OA.


Article
HLA- DRB GENOTYPING OF BRAIN ASTROCYTOMAS AMONG IRAQI PATIENTS
التنميط الجينى لمستضدات خلايا الدم البيضاء الصنف الثانى (HLA-DRB) لسرطان الدماغ النجيمى فى المرضى العراقيين

Authors: Ammira Hadi Al-Shabeeb عامرة هادي --- Nidhal Abdul Mohymen نضال عبد المهيمن محمد
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2008 Volume: 6 Issue: 2 Pages: 116-120
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background: The major histocompatiblity complex (MHC) refers to as human leukocyte antigen (HLA). The loss of HLA antigens by neoplastic cells is considerably important for tumor growth and metastasis and expression of certain certain HLA allels may predispose to have certain types of tumors. Objective: To investigate the genetic susceptibility of HLA-DRB1, DRB3, DRB4 and. DRB5 alleles to brain astrocytomas in Iraqi patients.Methods: HLA-DRB1, DRB3, DRB4 and, DRB5 allele polymorphisms were typed by polymerase chain- reaction with sequence-specific primers (PCR-SSP) in 30 unrelated patients astrocytomas and 17 unrelated normal control subjects. The association was measured by appropriate statistical tests.Results: Allele frequency (AF) of HLA-DRB1*10011 and DRB1*10012 was significantly decreased in brain astrocytomas patients than that in normal controls (0.53 vs 0.93) the odds ratio 8.76). There was no association between patients and controls in the rested HLA-DRB1 alleles. Conclusion: HLA-DRB1*10011 and DRB1*10012 alleles were less common in the patients with brain astrocytomas than in the healthy controls. Individuals carrying HLA-DRB1* 10011 and DRB1*10012 alleles might be considered as protective markers. These protective alleles; might have a role in the degree of malignancy of the tumors and its histological type.

خلفية الدراسة: تسمى مستضدات التوافق النسيجي (MHC) والخاصة بالخلايا البشرية بـ((HLA.فقدان الخلايا الورميه لهذه المستضدات , تعتبر ظاهرة مهمة لنمو و انتشار الورم . كما إن إنخفاض التعبير عن بعض من اليلات هذه المعقدات يمكن ان يهئ للإصابة بانواع معينة من الأورام.هدف الدراسة: التحري عن امكانية وجود علاقة الإصابة بسرطان الخلايا النجمية في الدماغ وبين وجود اليلات الصنف الثاني من معقدات التوافق النسيجي.المواد و طرائق العمل: تحديد وجود الليلات HLA-DRB1,DRB3,DRB4,DRB5 باستخدام تقنية تفاعل البلمره المتسلسل نوع PCR-SSP)) لدى 30 مريض مصاب بسرطان الخلايا النجمية في الدماغ إضافة إلى 17 شخص سليم كسيطرة.النتائج: وجد ان تكرار اللاليلي لجينات DRB1*10011,DRB1*10012 قد انخفض تواجدهما لدى المرضى مقارنة مع السيطرة (0.5 VS. 0.93). بينما لم نتوصل إلى أي علاقة بين المرض و الاليلات الاخرى.الإستنتاج: من الممكن اعتبار الاليلات DRB1*10011,DRB1*10012 من العوامل التي توفر حمايه ضد الإصابه بهذا الورم.

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