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Article
Lipoxidative and Glycoxidative Modifications of Erythrocytes-Proteins in Relation to Thyroid Status
التبدلات في تفاعلات اكسدة الدهون واكاسيد السكر مع بروتينات كريات الدم الحمر وعلاقتها بنشاط الغدة الدرقية

Author: Shatha Hussein Ali د.شذى حسين علي خضر
Journal: IRAQI JOURNALOF COMMUNITY MEDICINE المجلة العراقية لطب المجتمع ISSN: 16845382 Year: 2005 Volume: 18 Issue: 2 Pages: 191-196
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Abstract :Objective : Different mechanisms for lipid peroxidation activation in patients presenting with thyroid hormone abundance or deficiency have been analyzed . However, changes in metabolism associated with thyroid dysfunction through the glycoxidative reactions and its contribution to such enhancement of lipid preoxidation is assessed in this study. Methods:The study included seventy –two patient with either , hyperthyroidism , hypothyroidism or euthyroidism, in addition to 25 control subjects for the estimation in their erythrocytes :-1-concentration of the end product of lipid peroxidation (malondialdehyde- MDA).2-susceptibility to oxidative challenge (H2O2- induced methaemoglobin- Met-Hb).3-glycosylation of proteins (glycated haemoglobin- GHb).Results : The hyperthyroid patients group were presented with significantly elevated MDA levels (p < 0.001) among other thyroid disorders. Elevated Met-Hb levels were detected in different thyroid diseases. Moreover, significant modulation of glycated haemoglobin values was observed in both hyper- and hypothyroid patients (7.8 ± 1.8 , 5.9 ± 1.7 , respectively Vs control 5.1± 0.82).Conclusion : The changes in lipoxidative and glycoxidative modification of proteins in patients with thyroid pathology may have some clinical and biological implications. Key words : thyroid hormones , MDA, Glycated- Hb

ملخص:هناك فرضيات عديدة لتفسير اسباب زيادة سرعة اكسدة الدهون في الحالات المرضية المصاحبة لزيادة او نقصان نشاط الغدة الدرقية موضوعة حاليا قيد البحث . احتمال وجود علاقة للتغيرات الحاصلة في الايض المرتبطة بنشاط الغدة الدرقية بالتغيرات في ايض السكر وبالتالي امكانية مساهمته في تفسير زيادة اكسدة الدهون. تضمنت الدراسة الحالية اثنان وسبعون مريضا بين مصاب بزيادة او نقصان او اعتدال في نشاط الغدة الدرقية ، اضافة الى 25 شخصا طبيعيا كمجموعة مقارنة وذلك لغرض دراسة :1-تراكيز اكاسيد الدهون (المالوندايلديهايد) في كريات الدم الحمر .2-القابيلة للاكسدة بمادة بيروكسيد الهيدروجين لتكوين الميتوهيموكلوبين.3-تركيز البروتينات المتفاعلة مع السكر.اظهرت نتائج الدراسة زيادة نسبة المالوندايلديهايد في كريات الدم الحمر لمرضى فعالية الغدة الدرقية الزائدة وكذلك زيادة في تكوين الميتهيموغلوبين يصاحب الانواع المختلفة من الاختلال في وظائف الغدة الدرقية ، مع زيادة ملحوظة في درجة تفاعلات السكريات مع بروتينات كريات الدم الحمر في حالتي زيادة ونقصان نشاط الغدة الدرقية .يمكن الاستنتاج من هذه النتائج ان هناك تغيرات في تفاعلات اكسدة الدهون واكاسيد السكر مع بروتينات كريات الدم الحمر في الامراض المصاحبة لأختلال نشاط الغدة الدرقية والتي قد يكون لها بعض التطبيقات السريرية والحيوية لتحسين حالة المريض.


Article
SOME OBSERVATIONS ON THE OCCURRENCE OF β-THALASSEMIA IN MOSUL

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Abstract

Backgroundβ-thalassemia is found in Mediterranean, Saudia Arabia, Jordan, Egypt and Yemen. Survey in Iraq showed that β-thalassemia trait is carried by 4.5-5% of the population.ObjectiveTo determine factors that is associated with thalassemia in Mosul district. Methods105 thalassemia blood transfusion dependent children with the age of 2.5–18 years attending Ibn Al-Atheer Teaching Hospital in Mosul city during 2005 were included in this study, 45 healthy subjects served as control. Blood groups, Hb, and PCV were evaluated. SI, TIBC, and TS levels were also determined.ResultsThe occurrence of homozygous β-thalassemia is significantly higher in the offspring of first cousin marriages."O" blood group represents a highest percentage (42.8%) among the thalassemic patients. Hb, PCV and TIBC were below the control measurements. Whereas SI and TS were above that of the controls.ConclusionsConsanguinity seems to be increasing the size of the disease due to the limited health education of the parents about the disease.Key wordsThalassemia, Hb, PCV, SI, TIBC, TS

Keywords

Thalassemia --- Hb --- PCV --- SI --- TIBC --- TS


Article
A STUDY ON BLOOD PARAMETERS OF BARBUS XANTHOPTERUS , BARBUS SHARPEYI AND THEIR HYBRID
دراسة معالم دم اسماك الكطان والبني. (Barbus sharpeyi X Barbus xanthopterus ) وهجينهما

Author: Abed Al. Majeed H. Talal. Jassim H. Saleh. Amer A. .Jabir
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: Print:18138497 E; 24108456 Year: 2011 Volume: 10 Issue: 2 Pages: 13-18
Publisher: Basrah University جامعة البصرة

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Abstract

The blood parameters of Red Blood Cells for Barbus xanthopterus, Barbus sharpeyi and B. xanthopterus X B sharpeyi (hybrid )were mentionted. The dimentions of Red Blood Cells ( length and width) for the fish B.xanthopterus, B.sharpeyi and their hybrid were as follows: (6 and 4 microns; 5 and 3 microns; 6 and 4 microns). Hb content values for the above mentioned fish, were as follows: 5.18, 5.323 and 4 g /100 ml. PCV values for the above mentioned fish were as follows: 68.25% , 100% and 42.85714%. RBC numbers for the above fish were also mentioned as follows: 3450000, 3550000 and 3300000cell/1mm3.

درست معالم خلايا دم أسماك( تعداد كريات الدم الحمراء ، تقدير الهيموغلوبين ، مكداس كريات الدم الحمراء ، إبعاد الكريات الحمراء) لأسماك الكطان و البنى وهجينهما وكانت كالاتى׃ 3450000, 3550000, 3300000 كرية حمراء في الملمتر المكعب الواحد. اما محتوى الهيموغلوبين للأسماك المذكورة أعلاه فقد كانت كما يلى على التوالي׃ ، 5 •18 ، 323•5 و 4 غم 100 مل•أما بالنسبة لقيم مكداس كريات الدم الحمراء للأسماك المذكورة أعلاه فهي كما يلى على التوالي ׃25396• 68%،100 %و 42 %•وفيما يتعلق بأبعاد الكريات الحمراء( الطول و العرض) للكطان والبنى و هجينهما فقد كانت كما يلى على .(microns 4& 6)و(microns 3&5)،(microns4&6) التوالي.

Keywords

Red Blood Cells --- Hb --- B sharpeyi


Article
ASSESSMENT OF COMPLETE BLOOD COUNT IN PATIENTS WITH CORONARY ARTERY DISEASE

Author: Qais A. AL-Oqaily قيس عامر عبد الامير العكيلي
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2014 Volume: 12 Issue: 1 Pages: 82-87
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background:Several studies have demonstrated the role of hematological parameters like hemoglobin (Hb), white blood cells (WBC) count and platelet count in the assessment of patients with coronary artery disease; some studies suggest an additional role of platelet indices in the prognosis of patients with coronary artery disease.Objective:To assess complete blood count and platelet indices in patients with coronary artery disease.Methods:Blood sample of 42 newly diagnosed ischemic heart disease patients including 20 patients with myocardial infarction (MI) and 22 patients with unstable angina were studied. The control group included 15 individuals with no history of heart disease and a normal electrocardiogram. Clinical and laboratory information regarding age, sex, packed cell volume, WBC count, platelets count, platelet distribution width and platelet volume were obtained.Results:The frequency of diabetes and smoking was higher in patients with coronary heart disease in comparison to the control group (P < 0.05), while the frequency of hypertension was not significntly different. Significant difference in WBC count and Hb level of patients with coronary heart disease (P < 0.05), while no significant difference in the platelet count, platelet distribution width and mean platelet volume was found. In patients with unstable angina there was a significant difference in the Hb level (P < 0.05) while no significant difference in WBC count, platelet count, platelet distribution width and mean platelet volume was observed. In patients with myocardial infarction, there was a significant difference in the Hb level and the WBC count (P < 0.05), whereas no significant difference in platelet count, platelet distribution width and mean platelet volume was found.Conclusion:Hemoglobin level was significantly lower in patients with coronary artery disease while the mean level of WBC count was significantly higher than that of the control group. There was no significant difference in platelet count and platelet indices between those two groups. In patients with unstable angina the WBC count was not statistically different from that of the control group.Key words:Coronary artery disease, Hb, WBC, PDW, MPV.

Keywords

Coronary artery disease --- Hb --- WBC --- PDW --- MPV.


Article
HAEMATOLOGICAL STUDY OF -THALASSAEMIA AMONG IRAQI POPULATION
دراسة خصائص الدم لمرضى الثلاسيميا نوع بيتا بين المجتمع العراقي

Author: زهير محمد علي الاسدي1
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2008 Volume: 7 Issue: 2 Pages: 186-197
Publisher: Baghdad University جامعة بغداد

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Abstract

Blood samples of 80 clinically thalassaemic patients were collected from three thalassaemic centres in Iraq, in addition to 56 apparently healthy individuals were collected as a control group. Haematological examinations were applied for some parameters. The blood groups were determined for all samples, the relationships between blood groups and both gender and phenotypes of β-thalassaemia were studied. The results showed that there were no significant relationship at (p > 0.05) between blood groups and both parameters (gender and phenotypes) for patients in comparison with the control group. The estimations of Hb concentrations, quantitative and qualitative Hb analyses by Hb electrophoresis in order to determine the Hb types (HbF, HbA, and HbA2) and reticulocyte count, were adopted for 31 β-thalassaemic patients and 20 apparently healthy individuals. According to the haematological examinations data, the relationships of gender, blood groups, and phenotypes with Hb concentrations, Hb types, and reticulocyte count were studied.

جمعت عينات الدم من 80 مصاب سريريِاً بالثلاسيميا – بيتا من ثلاثة مراكز ثلاسيميا في العراق, فضلاً عن 56 شخص من الأصحاء ظاهرياً كمجموعة سيطرة. حددت فصائل الدم لكل العينات المشمولة بالدراسة, ودرست العلاقة بين فصائل الدم والجنس, والنمط المظهري للثلاسيميا – بيتا إذ أظهرت النتائج عدم وجود علاقة معنوية (p > 0.05) بين فصائل الدم وهذه المؤشرات المدروسة لمجموعة المرضى بالمقارنة مع مجموعة السيطرة. تم حساب تراكيز الهيموكلوبين و التحليل الكمي والنوعي للهيموكلوبين بأستخدام الترحيل الكهربائي لتحديد أنواعه HbA) و HbFو HbA2), وكذلك عد الخلايا الشبكية (Reticulocyte count) لـ 31مصاباً بالثلاسيميا – بيتا و20 من الأصحاء مظهرياً, وطبقاً لنتائج الفحوصات الدموية, درست العلاقات بين " الجنس وفصائل الدم والنمط المظهري" وبين "تركيز الهيموكلوبين وأنواع الهيموكلوبين وعد الخلايا الشبكية."


Article
The Prevalence of Anemia and Hookworm Ancylostoma Duodenale Infection in Mishamish Village, Al-Hashimya District, Babylon Province, Iraq

Authors: Zainab H. Kamil --- Karim H. Rashid --- Alaa S. Al-Awad
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2014 Volume: 11 Issue: 4 Pages: 891-900
Publisher: Babylon University جامعة بابل

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Abstract

This study was carried out to investigate the prevalence of anemia and its relation to hookworm Ancylostoma duodenale infection.Anemia is a common health problem all over the world and Iron Deficiency Anemia IDA is the most common and important type of anemia which is causally associated with developmental delay and with poor growth. Hookworm infection is one of the important common cause of IDA. The study included 138 subjects of different ages from Mishamish village of Al-Hashimiya District, Babylon Province, Iraq. Fecal specimens and blood samples were collected from each subject. Fecal samples were microscopically examined by direct and saturated salt floatation technique to investigate the hookworm infection. Red Blood Corpuscles Count (RBC), Hemoglobin concentration (Hb), Mean Corpuscular Hemoglobin (MCH), Mean Corpuscular Hemoglobin Concentration (MCHC), Haematocrit (Hct), Mean Corpuscular Volume (MCV), and Red cell Distribution Width (RDW) were measured by automated counter. Overall anemia percentage was 58.7%, and was 62.7% in males compared to 53.9% in females. The highest percentage of anemia (76%) was found in children of 3-5 years age group and decreased with age progress. It was found that 37% of anemic subjects were suffering from hookworm infection. Overall percentage of hookworm infection was 25.4%, and was 25.3% in males compared to 25.4% in females. The highest percentage of 36% was found in 3-5 years age group, and decreased as age increased. Hookworm infection caused significant decrease (P<0.001) in mean Hb, Hct and MCV, and significant decrease (P<0.01) in MCH, but it caused significant increase (p<0.005) in RDW of hookworm infected subjects. There was positive correlation (r = 0.94), (r = 0.97) between Hb concentration and, Hct & MCV respectively and negative correlation (r = - 0.97) between RDW and Hb in hookworm infected subjects.Hookworm infected subjects were severed from IDA especially among children and men. It should be get more attention to these groups of subjects. Treatment of anemia should be taken with the treatment of hookworm.

Keywords

anemia --- hookworm --- Hb --- MCH --- MCHM --- MCV --- RDW


Article
EFFECT OF PLASMID SIZE ON THE TRANSFORMATION OF E.COLI HB101
تأثير حجم البلازميد على تحول بكتريا القولون E.coli HB101

Author: Abdul Hussain Moyet Al-Faisal
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2006 Volume: 5 Issue: 1 Pages: 123-131
Publisher: Baghdad University جامعة بغداد

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Abstract

Plasmids are one of the most common vectors used in cloning. Many factorshave been repotted to affect the bacterial transformation by plasmids. Thispaper an attempt to find the effect of plasmid size on the transformationefficiency of E.coli HB101. Our results showed that the small sized Plasmidsmore effective in transforming of the bacteria E.coli HBCOL was) (4.1-4.8kbthan the large ones (5.7-6kb). The transformation efficiency with the small sizedplasmids reaches between 6×610-7×610 comparing with 4.1 ×105- 4.6×105 of thelarge sized plasmid.We conclude that the small sized plasmids are more suitable to use in cloningthan large sized plasmids.

تعتبر البلازميدات من المواد الوراثية الشائعة الاستخدام في الهندسة الوراثية وتختلف هذه في حجمهامن بلازميد الى نوع اخر. ومن خلال الابحاث العلمية التي أجريت سابقا فانه لوحظ وجود عوامل كثيرةتؤثر على فعالية هذه البلازميدات كنواقل في الهندسة الوراثية ومن هذه العوامل حجم البلازميدات وتأثيرذلك على كفاءة التحول في الخلايا المضيفة.6-4.10 كيلو قاعدة ) واستخدام ألسلاله ) pSp في هذا البحث تم استخدام أحجام مختلفة من البلازميدكمضيف . E.coli من بكتريا القولون HBIOIأوضحت النتائج بان حجم البلازميدات ذو أهمية كبيرة في تحديد كفاءة تحول بكتريا القولون حيث7 ) مقارنة x106 - 6x كانت كفاءة التحول عالية في البكتريا عند استخدام بلازميدات صغيرة الحجم ( 1066.0 كيلو قاعدة ) وهو - 4.6 في البلازميدات كبيرة الحجم ( 5.70 x105 - 4.1x بكفاءة تحول تبلغ 105مايوضح بان البلازميدات صغيرة الحجم أفضل كنوا قل في الهندسة الوراثية من تلك الكبيرة الحجم لأنهاتوفر كفاءة تحول اعلى .PDF


Article
Comparison between Cinnamoum cassia and Ocimum basilicum on some Biochemical and blood tests Rats
دراسة مقارنة نباتي القرفة والحبق على بعض المعايير الكيموحيوية والمعايير الدموية في الجرذان

Author: Dina Saadoun Diab دينا سعدون ذياب
Journal: Kufa Journal For Veterinary Medical Sciences مجلة الكوفة للعلوم الطبية البيطرية ISSN: 20779798 Year: 2011 Volume: 2 Issue: 2 Pages: 1-7
Publisher: University of Kufa جامعة الكوفة

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Abstract

Abstract: This study was designed to compare the effect of Cinnamoum with basil on some biochemical and blood tests in adult rats. Twenty one adult males used in this experimental divided into three equal groups: Control group (c) : which was given normal water and food.Treatment Group (F): which was given Cinnamoum with water orally (8 g / kg / B. W.) for 6 weeks.Treatment with basil (L): which was given aqueous extraction of basil orally (2 ml / kg / B. W.) for 6 weeks. Blood samples were taken direct from the heart in 2 , 4 , 6 weeks from experimental design to measure WBCs, Hb , Glucose , Cholesterol.The results of this study have appears that the Cinnamoum and basil play roles in decrease Glucose , Cholesterol of blood and necessary for diabetic disease and increase in WBCs, Hb.

الخلاصة: صممت هذه الدراسة لمقارنة تأثير كل من القرفة ونبات الريحان على بعض المعايير الكيموحيوية والدموية في الجرذان البالغة. استخدم في هذه التجربة 21 من ذكور الجرذان البالغة وقسمت عشوائياً الى 3 مجاميع:مجموعة السيطرة (س): اعطيت الماء والعلف الاعتياديين.مجموعة المعاملة بالقرفة (ف): أعطيت القرفة مع الماء عن طريق الفم وبجرعة 8 غم/كغم وزن الجسم لمدة 6 أسابيع.مجموعة المعاملة بنبات الريحان (ل): أعطيت مستخلص مائي لنبات الريحان عن طريق الفم وبجرعة 2 مل/كغم/وزن الجسم لمدة 6 أسابيع. تم أخذ عينات الدم من القلب مباشرة بالاسابيع 2، و4 ، و 6 من مدة التجربة لقياس كل من الكولسترول والكلوكوز وكريات الدم البيضاء وهيموغلوبين الدم.لقد أظهرت نتائج الدراسة أن القرفة والريحان يلعبان دوراً مهماً في التقليل من نسبة كولسترول الدم والكلوكوز فلذلك تعتبر القرفة ضرورية لمرض السكر، وكذلك يلعبان دوراً في رفع نسبة كل من كريات الدم البيضاء وهيموغلوبين الدم.


Article
Estimation of some T-helper 2 cytokines (IL-4 and IL-10) in HBV Infected Patients and Individuals Vaccinated with Recombinant HB Vaccine
تقدير بعض سايتوكاينات النوع الاول للخلايا المساعدة التائية (IL-8 and IL-4) في المرضى المصابين بالتهاب الكبد الفايروسي نمط ب والافراد الملقحين بلقاح التهاب الكبد نوع ب المحور

Author: Hiwa Abdul -Rahman Ahmed
Journal: Tikrit Journal of Pure Science مجلة تكريت للعلوم الصرفة ISSN: 18131662 Year: 2011 Volume: 16 Issue: 1 Pages: 5-9
Publisher: Tikrit University جامعة تكريت

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Abstract

infection, interleukin-4 (IL-4) and IL-10 are considered examples on T-helper 2 (Th2) cytokines which required for host antiviral immune response and involved in humeral immunity against HBV infection. This study was designed to estimation Th2 cytokines (IL-4 and IL-10) in HBV infected patients and individuals vaccinated with recombinant HB vaccine. Study groups were classified into patient group 35(15 acute (AH) and 20 chronic (CH)), 35 vaccinated group (20 responder (RD) and 15 Non-responder (NRD)) and 18 healthy control (HC) during May to November 2007. Blood samples were taken from patients and hospitals staffs to detection HBsAg, Anti-HBc Ab(IgM), Anti-HBs Ab, IL-4 level and IL-10 level in serum by enzyme linked immunosorbent assay (ELISA) test.The concentration of IL-4 and IL-10 levels in the AH group differed significantly compared with HC and CH patients (p<0.01) by F-test. LSD-analysis for IL-4 revealed same result only between HC and CH (P<0.008). Comparison between CH and both HC and AH, respectively revealed significant differences in IL-10 level (P<0.008) and (P<0.03) by LSD-test. F-test for IL-4 revealed non significant result (P>0.05) among RD, NRD and HN control in ≥30 and <30 years old, respectively, but inverse result was observed in IL-10 levels (p<0.05). LSD-test revealed (P>0.05) between all study group regarding IL-4, however the level of IL-10 were (P<0.014) and (P<0.01) in RD and NR groups among ≥30 and <30 years old, respectively. Indeed (P<0.03) between RD and HN groups in age <30 years. Conclusion: In this study significant elevation of IL-4 and IL-10 levels was observed in AH patients compared with CH patients and healthy control. Same result was seen in IL-10 among RD and NRD in ≥30 and <30 years old, as well as between RD and HN groups in age <30 years

للاستجابة المناعية الخلوية دور مهم في تنخر الكبد المرافق لمرض التهاب الكبد الفايروسي. يعتبر IL-4وIL-10 من الامثلة على السايكوتينات المنتجة من خلايا Th -2 و الذي يحتاجها المضيف كاستجابة مناعية ضد الفايروسات بالاضافة لدورهم في المناعة الخلطية ضد مرض التهاب الكبد الفايروسي. صممت هده التجربة لحساب سايتوكينات خلايا Th-2 خاصة IL-4وIL-10 للمرضى المصابين بالتهاب الكبد الفايروسي نوع ب والاشخاص الملقحين بلقاح HB.مجاميع المرضى صنفت الى مجموعة المرضى35 مريض (15 حاد( (ACو20 مزمن(Ch)) 35 هم مجموعة الملقحين (20 المستجيبين للقاح( (RDو15 غير المستجيبين للقاح (NRD)) بالاضافة الى 18 شخص كمجموعة سيطرة خلال الفترة من ايار الى تشرين الثاني 2007.اخدت نمادج الدم من المرضى والاصحاء من عدة مستشفيات منها مستشفى نانكلي واربيل ورزكاري التعليمي لتشخيص كل مما يلي HbsAgو Anti-HBc Ab(IgM)و Anti-HBs Abوكذلك تقدير مستويات IL-4و IL-10 في المصل بواسطة فحص الاليزا.مستويات كل من IL-4 و IL-10 للمرضى المصابين بالتهاب الكبد الفايروسي الحاد اختلفت معنويا بالمقارنة مع مرضى التهاب الكبد المزمن ومجموعة السيطرة (p<0.01) عند التحليل الاحصائي بالاختبار F, بينما الاختبار الاحصائي LSD لل IL-4ايضا اعطت نفس النتائج لكن بين HC و CH عند مستوى .(P<0.008) عند المقارنة بين CH و HCو AHعلى التوالي هناك فروقا معنويا في مستوى IL-10 (P<0.008) و (P>0.05)وذلك باستخدام التحليل الاحصائي LSD.اختبار F للIL-4 اعطت فرقا معنويا (p<0.05) عند مقارنة RDو NRDوالسيطرة الغير الملقحين في المجموعة العمرية 30 ≤ و < 30 سنة بالتتابع ولكن النتائج كانت مغايرة في مستوى IL-10 }(p<0.05). اختبار LSD للIL-4 لم تعطي فرقا معنويا (p<0.05) بين كل مجاميع الدراسة لكن كانت الفروق معنوية (P<0.014)و(P<0.01) عند مقارنة RDو NRDوالسيطرة الغير الملقحين في المجموعة العمرية 30 ≤ و < 30 سنة بالتتابع اضافة لدلك كانت الفروق معنوية(P<0.03) بين مجاميع RD و HN في المجموعة العمرية30 < سنة. الاستنتاجات : توصلنا في هذه الدراسة ان هناك ارتفاعا معنويا في مستوى IL-4وIL-10 في مصول المرضى المصابين بالتهاب الكبد الفايروسي الحاد بامقارنة مع المرضى المصابين بالتهاب الكبد الفايروسي المزمن ومجموعة السيطرة. نفس النتائج سجلت بالنسبة لمستوى مستوى IL-10 في مجاميع RDو NRD في المجموعة العمرية 30 ≤ و < 30 سنة بالتتابع كذلك بين مجاميع RD و HN في المجموعة العمرية30 < سنة.

Keywords

IL-4 --- IL-10 --- HBV --- recombinant HB vaccine.


Article
Evaluation of (HPLC) Patterns of Sickle Cell Anaemia Patients in Comparison with Apparently Healthy Individuals

Author: Zuhair Mohammad Ali Jeddoa
Journal: Karbala Journal of Medicine مجلة كربلاء الطبية ISSN: 19905483 Year: 2011 Volume: 4 no 1 Issue: 9 Pages: 980-987
Publisher: Kerbala University جامعة كربلاء

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Abstract

background: Sickle cell haemoglobin (HbS) results from an autosomal recessively inherited mutation in which the amino-acid glutamine is replaced by valine at position 6 in the beta globin chain of haemoglobin (Hb). Sickle cells have a reduced deformability and are easily destroyed, causing occlusion of the microcirculation and a chronic haemolytic anaemia with a median Hb concentration level of about 9 g/dl. Routine electrophoresis methods and High performance liquid chromatography (HPLC) were used to screen normal and variant Hb, and allowed the verification of the Hb observed with electrophoresis and precise quantification of their proportion.Objectives: 1- This study aimed to evaluate the chromatographic pattern of Hb types (HbA, HbF, HbA2 and HbS) of sickle cell anemia patients in comparison with the apparently healthy individuals.2- To study the Hb chromatographic patterns according to the gender, age and blood groups.3- To evaluate the efficiency of variant Hb testing system in detection of HbS type of sickle cell anaemia patients.Methods: A total of eighty four sickle cell anemia patients who were attending to theIbn Al-Baladi pediatrics hospital (in Baghdad) and Kerbala teaching pediatricshospital, the samples were (39) males and (45) females , from April of 2006 toFebruary of 2007 . And thirty seven of case controls with matched age and sex wererandomly selected from apparently healthy individuals. High performance liquidchromatography (HPLC) was adopted to determine the different types of Hb forpatients and control groups using variant Hb testing system which depend upon theseparation and quantification of Hb types by high performance liquid chromatographytechnique.Results: The study of Hb chromatographic patterns of samples revealed that there were no significant differences (p ≥ 0.05) between the values of HbA2 for the patients and control groups and there were significant differences (P≤ 0.05) for HbF and highly significant differences (P≤ 0.01) for HbA and HbS percentages of patients in comparison with the control group. The results of Hb chromatographic patterns of samples according to the gender revealed that there were no significant differences at (p≥ 0.05) between males and females within patient and control groups. The results of Hb chromatographic patterns of Hb types for patients and control groups according to the age groups revealed heterogeneous results with highest HbF, HbA, and HbA2 percentages of patients were (10.83±3.32, 57.6±7.33, and 4.22 ±1.88) respectively in age group less than five years old, while it was (54.33±8.9) for HbS type in age group (6-10) years old. As for control group, the highest HbF percentages was (8.2±4.09) in age group less than five years old, for HbA was (87.22±5.86) in age group (6-10) years old, for HbA2 was (3.6±0.23) in age group (11-15) years old, and for HbS was (0.3±0.05) in age group less than five years old. Finally, the results also showed that there were no significant differences at (P ≥ 0.05) for Hb chromatographic patterns of different Hb types percentages according to the blood groups of studied samples within group (patients or controls).Conclusion:1- The study of Hb chromatographic patterns is useful for the diagnosis of sickle cell anaemia.2- There are no significant effects of gender and blood groups on the chromatographic patterns of different Hb types of sickle cell anaemia patients in comparison to the apparently healthy individuals.3- HPLC is an excellent, powerful diagnostic tool for the direct identification of HbS.

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