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Article
Detection of Low Risk Genotypes of Human Papilloma Virus 6 and 11 in Patients with Cervicitis by Real Time PCR Assay
تحديد النمط منخفض الخطورة لفايروس الورم الحليمي البشري 6 و 11 في مرضى التهاب عنق الرحم بواسطة قياس Real Time للتفاعل التضاعفي المتسلسل الكمي

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Abstract

Background: The human papillomavirus (HPV) infection is one of the most prevalent sexually transmitted disease. The low risk genotypes of Human Papilloma Virus such as 6, 11, 40, 42, 43, 44, 54, 61, 70, 72 and 81 are mainly associated with benign genital warts and rarely leading to cancer. This study has been undertaken for detection of the occurrence of HPV 6 and 11 in patients with cervicitis depending on Real-Time PCR assay.Patients and methods: A total of 100 females (80 patients and 20 healthy females); ages (range from19- 62 years) were included and studied during the period from December 2014 to April 2015. They included 80 (80%) patients were diagnosed at private clinic in Ramadi from patients with different cervical lesions and healthy females: (health individuals 20 (20%).Real time-PCR technique was done for DNA extracted from these samples. Result: The mean range for age showed that patients with cervical lesion from patient with pap smear samples were (31.63 ± 6.12), polyp samples (30.32 ± 8.35), ulcer samples was (31.52 ± 10.70), while the wart samples (30.19 ± 7.38), Leukemia patients have wartic lesion samples was (30.50 ± 2.52). No significant difference between age and location results for the other groups (P > 0.05). The purity of DNA from clinical specimen was (1.39 ± 0.23). There was extracted DNA from clinical specimens regarding purity or DNA concentration at which (P > 0.05). These results showed that the rate of HPV 6/11 DNA detection in different clinical samples were statistically significant for the patient group (P = 0.0005). In addition to that, the distribution of positive and negative HPV 6/11 between patients and control is also significant (P=0.002).Conclusion: The low risk genotypes of HPV 6 and 11 have a significant role in patients with cervicitis. The prevalence rate of Low-Risk HPV genotype 6 was found to be (17.5%), while that of HPV genotype 11 was (12.5%).

الاصابة بفايروس الورم الحليمي البشري هي واحدة من أكثر الأمراض المنقولة جنسيا انتشارا. ترتبط الأنماط الجينية منخفضة الخطورة من فايروس الحليمي البشري مثل 6 و 11 و 40 و 42 و 43 و 44 و 54 و 61 و 70 و 72 و 81 بشكل رئيسي بالثآليل التناسلية الحميدة ونادرا ما تؤدي إلى السرطان. وقد أجريت هذه الدراسة للكشف عن حدوث فيروس الورم الحليمي البشري 6 و 11 في المرضى الذين يعانون من التهاب عنق الرحم اعتمادا على قياس Real time للتفاعل التضاعفي المتسلسل الكمي.من مجموعه 100 أمراءه (80 مريضة و 20 أمراءه صحية)؛ وأعمارهم ( تتراوح من 19-62 سنه)المتضمنة الدراسة خلال الفترة من ديسمبر 2014 الى ابريل 2015.المتضمنه 80(80 ٪) تم تشخيصهم في عيادة خاصة في الرمادي من المرضى الذين يعانون من آفات عنق الرحم والإناث الأصحاء. والاشخاص الاصحاء 20 (20٪) وقد تم هذا بتقنيه Real time للتفاعل التضاعفي المتسلسل الكمي للحمض النووي المستخلص من هذه العينات.أظهرت النتائج أن معدل المرضى الذين يعانون من آفة عنق الرحم من المرضى الذين لديهم عينات مسحة pap كانت (31.63 ± 6.12)، وعينات الأورام (30.32 ± 8.35)، وكانت عينات القرحة (31.52 ± 10.70)، في حين أن عينات الثآليل (30.19 ± 7.38 )، مرضى اللوكيميا لديهم عينات الآفة الثؤلية كانت (30.50 ± 2.52). لا يوجد فرق كبير بين نتائج العمر والمكان للمجموعات الأخرى (P> 0.05). كانت نقاوة الحمض النووي من العينة السريرية (1.39 ± 0.23). تم استخراج الحمض النووي من العينات السريرية فيما يتعلق بالنقاء أو تركيز الحمض النووي الذي (P> 0.05). وأظهرت هذه النتائج أن معدل فيروس الورم الحليمي البشري 6/11 للكشف الحمض النووي في عينات سريرية مختلفة كانت ذات دلالة إحصائية لمجموعة المرضى (P = 0.0005). وبالإضافة إلى ذلك، فإن توزيع فيروس الورم الحليمي البشري إيجابي وسلبية 6/11 بين المرضى والسيطرة هو أيضا فرق معنوي (P = 0.002). إن النمط الجيني منخفض المخاطر من فيروس الورم الحليمي البشري 6 و 11 لها دور كبير في المرضى الذين يعانون من التهاب عنق الرحم. و معدل انتشار منخفضة المخاطر فيروس الورم الحليمي البشري النمط الجيني 6 لتكون (17.5٪)، في حين أن النمط الفيروسي فيروس الورم الحليمي البشري 11 كان (12.5٪).


Article
The relation of Echocardiographic findings to pulmonary Function tests in patients with Chronic obstructive Pulmonary Disease

Authors: Ammar A. Ismael --- Muataz F. Hussain معتز فوزي الحديثي --- Kassim M. Sultan قاسم محمد سلطان
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2009 Volume: 51 Issue: 1 Pages: 17-22
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Chronic obstructive pulmonary disease (COPD) is a common disease and it accounts for over 10% of all hospital medical admission. Cigarette smoking is the most important risk factor. Pulmonary arterial hypertension (PHT) is a common complication of COPD and the increase in pulmonary artery pressure is often mild to moderate. The presence of pulmonary arterial pressure and its severity is readily and reliably determined by transthoracic echocardiography in majority of COPD patients.
Patients and Methods: This study included 55 patients with mean age 65.6 ±8.2 years .The mean duration of symptoms was 18 ±10 months. 32 patients (58%) were current smoker, 18 patients (33%) were ex-smoker and 5 patients (9%) were non smoker. The mean intensity of smoking for smoker was 49.5 ± 22.2 pack- years. For all patients, history, clinical examination, ECG, CXR, and routine blood tests were done. For all patients pulmonary function tests were done and patients were classified according to GOLD criteria into 4 stages. Echocardiography was done for all patients; ventricular and atrial dimensions were taken, and using Doppler technique to detect tricuspid and pulmonary regurgitation, estimation of pulmonary artery systolic and/or diastolic pressure using special formulas was undertaken.Results: Tricuspid regurgitation (TR) jet was found in 70.9% of patients with COPD. Increased pulmonary artery systolic pressure was found in 51% of patients with TR (36% of total patients) and increased pulmonary vascular resistance was found in 48.7% of patients with TR (34.5% of total patients). There were significant associations between echo findings of increasing RV size, TR, increased pulmonary arterial pressure and pulmonary vascular resistance with decrease in FEV1, FEV1/VC and oxygen saturation. There was no significant association with decrease in vital capacity.Conclusions: There is a high prevalence of pulmonary arterial hypertension with increasing severity of chronic obstructive pulmonary disease.


Article
Molecular Detection and Genotyping of Human Papilloma Virus Infections in Iraqi Patients with Esophageal Carcinoma.

Authors: Tural Y. Bakir * MSc --- Saad H. Mohammed Ali ** PhD --- Intisar M. Jumaa * PhD
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2009 Volume: 51 Issue: 2 Pages: 204-210
Publisher: Baghdad University جامعة بغداد

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Background: Molecular DNA hybridization has confirmed more than 120 different human papilloma virus (HPV) genotypes. A small group of them have high- risk oncogenic potential. Many studies have described an association of such high risk-HPV genotypes with a variety of esophageal benign tumors as well as malignant squamous cell carcinomas.Patients and Methods: A total number of 90 tissue specimens were collected from 50 patients with esophageal squamous cell (SCC), adenocarcinoma (AC) and carcinoma in situ (CIS); 20 patients with squamous acanthosis (SA); and 20 individuals with apparently-healthy esophageal tissues (AHET). The molecular detection methods for HPV detection and genotyping were performed by in situ hybridization using cocktailed- and specific high- risk HPV DNA probes, respectively.
Results: The overall percentage of HPV in the total group of esophageal carcinoma was 20%.The percentage of HPV DNA in the subgroup of SCC and AC was 26.7% and 13.3%, respectively,. However, neither HPV DNA was detected in CIS subgroup nor in both control groups (SA and AHET).The overall genotyping results showed that HPV 18 constituted the majority of the detected high-risk oncogenic HPV genotypes, followed by HPV 16 then HPV 31/33.<.Conclusions: Despite the low prevalence of HPV infection and rarity of invasive esophageal carcinoma in the general Iraqi population, the detection of high percentage of such high oncogenic risk- HPV genotypes in these carcinomas indicating for a relevant importance in esophageal carcinogenesis.


Article
Detection and Genotyping of Human Papilloma Virus-Associated

Author: Saad H. Mohammed Ali* MBChB , MSc , PhD (Medical and Molecular Virology). د. سعد محمد
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2009 Volume: 51 Issue: 3 Pages: 276-282
Publisher: Baghdad University جامعة بغداد

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Summary:Background: Molecular DNA hybridization has confirmed more than 120 different human papilloma virus (HPV) genotypes. A small group of them have high- risk oncogenic potential. Many studies have described an association of such high risk-HPV genotypes with a variety of oral lesions including squamous cell carcinoma, leukoplakia, and lichen planus. .Materials and Methods: A total number of 42 tissue specimens, representing 27 patients with oral lichen planus and 15 apparently-healthy oral tissues, were included in this study. The molecular methods for HPV detection and genotyping were performed by in situ hybridization(ISH) using cocktailed- and specific high- risk HPV DNA probes, respectively.Results:The overall percentage of HPV in the total group of patients with oral lichen planus (OLP) was 33.3 %. Negative HPV DNA- ISH reactions were detected in all tissues of the control group. The overall genotyping results revealed HPV 16- DNA in all HPV- positive oral lichen planus tissues , while none of these OLP tissues showed ISH reactions for HPV 18-DNA or HPV 31/33-DNA.Conclusions: The significant incidence of such high oncogenic HPV genotype in those patients with oral precancerous lesions could have a relevant importance along its pathogenesis and the multi-steps oral carcinogenesis , HPV-16-associated oral lichen planus that has mostly previliged the site of cheek mucosa represents a herald indicator for spread of such sexually important transmitted infection among Iraqi general population.Key Words: Oral lichen planus; Human Papilloma Virus; In Situ Hybridization


Article
Genotyping of Human Papilloma Virus Infections and Phenotyping of Tumor Infiltrating Lymphocytes in Iraqi Patients with Uterine Cervical Neoplasia

Author: Majid Mohammed Mahmmod Al-jewari
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2007 Volume: 6 Issue: 4 Pages: 362-373
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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ABSTRACT:BACKGROUND:Among most basic and clinical investigations for detection and genotyping HPV, only In Situ Hybridization (ISH) is effective in studing the relation of HPV genome to that histopathological entity. Tumor Infiltrating Lymphocytes (TILs) represent the local cellular immune response against both cervical cancer and the associated HPV.OBJECTIVE:This study is aimed to investigate ISH in detection and genotyping of HPV and immunohistochemical (IHC) study in phenotyping of TILs in Iraqi women with different grades of cervical neoplasia.MATERIALS AND METHODS:This retrospective research included a total number of 129 cervical tissue blocks , 64 with invasive and 27 with pre-invasive cervical neoplasia,12 with condylomata acuminata,11 with chronic cervicitis and 15 with apparently- healthy cervices .Molecular detection and genotyping of HPV DNA were performed by using ISH whereas immunophenotyping was done by IHC using monoclonal antibodies for that specific CD markers representing some of TILs.RESULTS:The overall prevalence of HPV DNA in total group of invasive cervical neoplasia was 28.4% , 12 out of 48(25%) with squamous cell carcinoma(SCC) and 8 out of 16(50%) with adenocarcinoma (AC). HPV 16 constituted (58.3%) of HPV- positive invasive cervical SCC , where as HPV 31/33 and lastly HPV 18 constituted (25%) and (16.7%) , respectively .In AC group, HPV 16, 18, 31/33 occupied 50% , 25% , 12.5% of this group, respectively .The prevalence of HPV DNA in pre-invasive cervical neoplasia group was 22.2%. While no case with HPV 18 was detected , HPV 16 and HPV 31/33 genotypes occupied 40% and 60% , respectively .Although CD3+,CD4+and CD8+ lymphocytes showed high mean values , significant differences present only between HPV DNA- positive tissue blocks and control group (p<0.05) . The CD20+and CD56+ lymphocytes showed low mean values.Compared to control group,however,statistical analysis showed significant differences (p<0.05) regarding CD20+ and non-significant regarding CD56+ lymphocytes .CONCLUSION:Using ISH proved more powerful and sensitive tool in revealing precised effects of HPV in cervical neoplasia compared to histopathological examination .High mean values of CD4+ and CD8+ versus low mean values of CD20+ and CD56+ lymphocytes could reflect an important and specific role of these cellular responses against HPV viruses during initiation and progression of HPV-associated cervical cancers.


Article
P53-tumor suppressor gene overexpression in human papilloma virus-infected patients with oral squamous cell carcinoma

Authors: Saad Hasan Mohammed Ali سعد حسن محمد --- Athraa Yahya Al-Hijazi عذراء يحيى الحجازي --- Basim M.Khashman باسم خشمان
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2011 Volume: 23 Issue: special issue Pages: 70-76
Publisher: Baghdad University جامعة بغداد

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Background: Via molecular DNA hybridization, more than 120 different genotypes of human papilloma virus havebeen confirmed. Many studies have described an association of high risk-HPV genotypes and overexpression ofmutated P53 gene with a variety of oral benign tumors as well as malignant squamous cell carcinomas. This studyaimed to: 1) Determine the prevalence of HPV DNA in archival tissue specimens with a range from apparentlyhealthy tissue to invasive oral S.C.C by using one of the recent versions of insitu hybridaization. 2) Define thegenotypes of the obtained HPV and to find out rational significance and relation of such genotypes to the severity ofunderlying lesions. 3) Study the correlation of over expressed products of mutant p53 genes with HPV-negative andHPV-related oral cancers.Materials and methods: A total number of 72 tissue specimens were collected from 41 patients with oral squamouscell (OSCC) and 31 individuals with apparently-healthy oral tissues (AHOT). The molecular detection methods for HPVdetection and genotyping were performed by in situ hybridization using cocktailed- and specified high- risk HPV DNAprobes, respectively. Immunohistochemical method was used to demonstrate the prevalence of P53 overexpressionin those oral cancers.Results: Among oral OSCC group, 16 archived tissue blocks were found to contain HPV DNA related to thecocktailed HPV genotypes. This result constituted 39% of the total oral SCC screened for HPV DNA. HPV-18 positiveoral SCC tissue blocks constituted (68.75%) whereas HPV genotypes 16& 31/33 constituted (43.75%) & (12.5%),respectively. Mixed infection of HPV genotypes was found in 31.3%. Interestingly, HPV DNA detection wasdocumented in 3.2% of those appeared as healthy tissues on histopathological examinations. Among oral SCCgroup, 22(53.7%) showed over expression of P53 tumor suppression gene. Interestingly, the co-occurrence of mutatedP53 overexpression and high oncogenic risk HPV genotypes was documented in 75% of Iraqi patients with OSCC.Conclusions: The significant prevalence of high oncogenic HPV genotypes detection in those patients with OSCCindicates a herald marks for the spread of such important sexually transmitted infection among Iraqi generalpopulation. Both of mutated p53 genes as well as high-oncogenic risk HPV genotypes could play an important role inoral carcinogenesis.


Article
Human Papilloma Virus in a Sample of Iraqi Women with Normal and Abnormal Pap Smear

Authors: Nada Salih Ameen --- Dalia Thamer Ahmmad
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2014 Volume: 13 Issue: 2 Pages: 211-218
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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ABSTRACT: BACKGROUND:World wide, cervical cancer is one of the most common cause of death from cancer in women, HPV testing have a key role in primary cervical screening and the currently accepted view is that HPV is an essential factor in the causation of the disease.OBJECTIVE: The objective of the study is to outline the presence of HPV infection in our community through sample of Iraqi's women who attended Central Health Laboratories with normal and abnormal Pap smear during the period from August 2006 to December 2008.METHODS: The data were collected from records of the laboratory which includes the information about patients who were referred to the central health laboratories to do HPV test , these data includes: age of the patients , marital state, years after marriage which represents the age of first sexual acts , also the records includes the smoking state, Pap smear results and lastly the HPV test results. HPV testing was done by PCR method , which initially required DNA extraction by MICROGENO DNA from AB Analitica , then DNA Amplificaion by PCR , and finally Electrophoresis for reading the UV light and translating it into certain molecular weight by Molecular Weight Markers.RESULTS:The percentage of HPV infection was 15.65 % , the percentage of positive test was 20% among those aged between 20-30 years old , no viruses had been detected above 50 years old . All the cases that have been examined were married and had 15% infection rate among them . The maximum infection rate 20% was among those who had sexual activity for 5-9 years. 91.6% of the infected women were smokers. 5.5% of the women with negative cytological results was infected, 26.3 % of those with ASCUS, 43.3% of those women with CIN I, 45.5% of those with CIN II , and 57% of those with CIN III ; the highest infection rates were 100% among patients with micro-invasive cancer. CONCLUSION: -High rate of HPV infection is associated with age , smoking and multiple sexual partners(married more than once). - High frequency of detection of oncogenic HPV infection are associated with increasing grade of cervical lesion.


Article
Genotyping of High-risk Human Papilloma virus (HPV) among Iraqi women in Baghdad by Multiplex PCR
كشف فايروس الورم الحليمي البشري عالية الخطورة بين النساء العراقيات في بغداد باستخدام تقنية تفاعل التضاعف المتعدد لسلسلة الدنا

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Human papilloma virus (HPV) infection is a causative factor for cervical cancer. Early detection of high risk HPV types might help to identify women at high risk of cervical cancer. The aim of the present study was to determine the occurrence ofhigh risk HPV infection in population of Iraqi women in Baghdad by using Multiplex PCR determine the percentage and genotyping of Human Papilloma Virus and to put the best prevention and control program in Iraqi women. Study started at January 2009 to March 2010, cervical samples were collected from 856 women aged 16–70. HPV DNA amplification was performed using HPV High Risk Typing PCR Kit test for qualitative detection and genotyping of HPV types 16, 18, 31, 33, 35, 39, 45, 52, 56, 58, 59, 66 in the cervical swabs. HPV was detected in 106 ( 12,38% ) of the study population, with a range of 16-70 years age groups. Results showed that the overall HPV prevalence twelve genotypes were identified, including HPV-33 (18.60%), HPV-35 (18.60%), HPV-56 (18.60%) ,HPV-39(10.85%), HPV-52 (10.08%), HPV-18 (7.75%), HPV-16 (4.65%), HPV-59 (4.65%), HPV-58(2.32%), HPV-31 (1.55%), HPV-45(1.55% ) and HPV-66( 0.77%). Of 856, 218 women was also tested by pap smear , with normal cytology was 198 ( 90.83%), 24(12.12%) of them were HPV positive, those with abnormal cytology was 20 (9.17 %), 5( 25%)of them was HPV positive. In this study unlike other epidemiological studies, HPV33,35,56 was the most frequent type (55.8%) in Baghdad, followed by HPV39, HPV52, HPV18, HPV16.

فايروس الورم الحليمي البشري (HPV) هوالعامل المسبب لسرطان عنق الرحم، وان الكشف المبكر عن أنواع فايروس الورم الحليمي البشري عالي المخاطر قد تساعد على تحديد النساء المعرضات عالية الخطورة من سرطان عنق الرحم. وكان الهدف من هذه الدراسة لتحديد خطر حدوث عدوى فايروس الورم الحليمي البشري في عدد السكان من النساء العراقيات في بغداد باستخدام PCR لتحديد نسبة والتنميط الجيني لفايروس الورم الحليمي البشري(HPV) ووضع أفضل برنامج للوقاية والسيطرة على المرض، بدأت الدراسة في كانون الثاني 2009 إلى مارس 2010، تم جمع عينات من عنق الرحم من856 امراة والتي تتراوح أعمارهم بين -1670 سنة، وتم إجراء كشف فايروس الورم الحليمي البشري HPV DNA باستخدام تقنية تفاعل التضاعف المتعدد لسلسلة الدنا (Multiplex- PCR) للكشف النوعي والتنميط الجيني لأنواع فايروس الورم الحليمي البشري 16، 18، 31، 33، 35، 39، 45، 52، 56، 58، 59،66 في مسحات عنق الرحم. النتائج: فيروس الورم الحليمي البشري تم الكشف106 (12.38)% حالة من الدراسة الحالية، مع مجموعة واسعة من الفئات العمرية 16-70 سنة. وأظهرت النتائج أن معدل انتشار فايروس الورم الحليمي البشري (الاثنى عشر من الأنماط الجينية ): فيروس الورم الحليمي البشري (18.60%) 33-، فيروس الورم الحليمي البشري35- (18.60%)، فيروس الورم الحليمي البشري(18.60 %) 56-، فيروس الورم الحليمي البشري (10.85 %) 39 - فيروس الورم الحليمي البشري 52 (10.08%) ، فيروس الورم الحليمي البشري18- (7.75%) فيروس الورم الحليمي البشري16- (4.65%) فيروس الورم الحليمي البشري59- (4.65%) فيروس الورم الحليمي البشري (2.32%)58 -، فيروس الورم الحليمي البشري %) 31- (1.55فيروس الورم الحليمي البشري45- (1.55%) وفيروس الورم الحليمي البشري66- (0.77%) من 856. تم اختبار218 امرأة أيضا عن طريق مسحة pap smear وقد اظهرت النتائج ان 198 (90.83%) كانت حالة الطبيعية, 24 (12.12%) منهم كانت إيجابية لفيروس الورم الحليمي البشري و20 (9.17% ) كانوا حالة غير طبيعية, 5%) (25 منهم كانت إيجابية لفيروس الورم الحليمي البشري. في هذه الدراسة على عكس الدراسات الوبائية الأخرى 56، 35 ، HPV33، كان النوع الاكثر شيوعا (55.8%) في بغداد، تليها HPV-39 ، HPV-52، HPV-18،HPV-16 .


Article
Association of Human Papillomavirus Infections with Male Infertility in Diyala-Iraq
العلاقة بين اخماج فيروس الورم الحليمي البشري مع عقم الذكور في ديالى العراق

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Abstract

Abstract:Background: Estimates suggest that worldwide between 3-7% of all heterosexual couples or women have an unresolved problem of infertility. 20-30% of infertility cases are due to male infertility. The prevalence of human papilloma virus (HPV) sperm infection ranges between 2 and 31% in men from general population and 10 - 35.7% in men with unexplained infertility.Objectives: This study was designed to figure out the association of HPV type 18, 16, 11, and 6 in men with infertility in Diyala province-Iraq.Subjects and methods: Seminal fluid samples were collected from 90 men who were subdivided equally into oligospermia, azoospermia, and healthy individuals. The age range was 22-48 years with a mean age ± SD was 29.28± 5.78 years. Socio-demographic information were also collected by direct short interview. Complete seminal fluid analysis was done according to the World Health Organization (WHO) guidelines. HPV type 18, 16, 11, and 6 were detected quantitatively and qualitatively in semen samples using the real time PCR kit (AmpliSens® HPV 16/18-FRT). Human privacy was respected by taken participant's consent. Statistical analyses were done using the IBM Statistical Package of Social Science (SPSS), Version 21 in association with Microsoft Excel 2010. P values less than 0.05 were considered significant. Results: The infection rate of HPV type 18, 16, 11, and 6 were 1.1%, 6.7%, 10.0% and 1.1% respectively. The mean titer of HPV type 16 and type 18 were 188.2 and 3.0 copies/milliliter of seminal fluid respectively. The highest infection rate was in oligospermia (30%) followed by azoospermia (20%) and the viral infections were mainly affects the 26-32 years age group. HPV type 11 was significantly impaired the sperm activity (P= 0.009) and HPV type 16, 11, and 6 were significantly affects the sperm morphology (P= 0.004, 0.001, and 0.04) respectively.Conclusion: Although HPV infections are relatively low among men with infertility; however, its effects on sperm parameters are worthy to suggest viral screening of men, at least, those couples with unexplained infertility.

الخلاصة:تمهيد: تشير الاحصائيات إلى أن في جميع أنحاء العالم يوجد بين 3-7٪ من الأزواج أومن النساء ممن لديهم مشكلة لم تحل بعد من العقم, وان 20-30٪ من حالات العقم بسبب العقم عند الذكور.ان انتشار فيروس الورم الحليمي البشري في الحيوانات المنوية تتراوح بين 2 - 31٪ لدى الرجال من عامة السكان و 10 – 35,7٪ لدى الرجال الذين يعانون من العقم.اهداف الدراسة: صممت هذه الدراسة لمعرفة مدى انتشار اخماج فيروس الورم الحليمي البشري نوع 18، 16، 11، و 6 في الرجال الذين يعانون من العقم في محافظة ديالى في العراق.المرضى وطرائق العمل: تم جمع عينات السائل المنوي من 90 رجلا تم تقسيمهم بالتساوي إلى قلة النطاف (oligospermia) ، و انعدام النطاف (azoospermia) ، والأفراد الأصحاء. تراوحت اعمار الرجال الدخلين في الدراسة 22-48 سنة مع متوسط العمر ± سد 29,28 ± 5,78 سنة. كما تم جمع المعلومات الاجتماعية الديموغرافية من خلال مقابلة قصيرة مباشرة. تم إجراء تحليل السائل المنوي الكامل بحسب ما اقرته منظمة الصحة العالمية. تم الكشف عن فيروسات الورم الحليمي البشري نوع 18 و 16 و 11 و 6 كميا ونوعيا في عينات السائل المنوي باستخدام التقنية الجزيئية تفاعل البلمرة المتسلسل (AmpliSens® HPV 16/18-FRT). واحترمت خصوصية الإنسان بموافقة المشارك حتما. تم إجراء التحليلات الإحصائية باستخدام حزمة الإحصائية للعلوم الاجتماعية (SPSS)، الإصدار 21 بالتعاون مع ميكروسوفت إكسيل 2010. واعتبرت قيم P أقل من 0.05 معنوية.النتائج: كان معدل اانتشار الاخماج بنوع فيروس الورم الحليمي البشري 18 و 16 و 11 و 6٪ 1.1٪ و 6.7٪ و 10.0٪ و 1.1٪ على التوالي. كان متوسط معيار ((Titer فيروس الورم الحليمي البشري من النوع 16 و 18 188,2 و 3,0 نسخة / ملليلتر من السائل المنوي على التوالي. وكان أعلى معدل للعدوى فيحالات فلة النطاف oligospermia (30٪) تليها انعدام النطاف azoospermi (20٪)، وكانت الاخماج الفيروسية تؤثر بشكل رئيسي على الفئة العمرية 26-32 سنة. واظهرت الاصابة فيروس الورم الحليمي البشري نوع 11 ضعفت بشكل معنوي في حركة الحيوانات المنوية (P = 0.009) ونوع فيروس الورم الحليمي البشري 16، 11، و 6 تبين بانه يؤثر بشكل معنوي على التشوهات المظهرية للحيوانات المنوية (P= 0.004, 0.001, and 0.04) على التوالي.الاستنتاج: على الرغم من نسب انتشار اخماج فيروس الورم الحليمي البشري منخفضة نسبيا بين الرجال الذين يعانون من العقم. ومع ذلك، فان تاثيره على مفردات الحيوانات المنوية هي جديرة بشمول اولائك


Article
A STUDY ON HUMAN PAPILLOMAVIRUS USING IN SITU HYBRIDIZATION TECHNIQUE AND ITS ROLE IN CERVICAL NEOPLASIA
دراسة حول الفيروس الحليمي البشري باستعمال تقانة التهجين الموضعي و علاقته بسرطان عنق الرحم

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Abstract

Background: clinical epidemiological studies have shown that human papillomaviruses play major role in the development of different types of cervical lesions, are therefore considered as the major infectious etiological agents of genital lesions and cancer. Objective: to determine the prevalence of HPV DNA by using in situ hybridization technique among archival tissue specimen of the uterine cervical lesions and normal cervical postmortem tissue biopsies.Material & Methods: Eighty cervical tissue samples were included in this study. 70 archival tissue biopsy samples comprised a risk group for HPV infection and / or cervical neoplasia; these were selected for the years from 1998 to 2005 from histopathology files of Al-kadhimiya teaching hospital, Al-Ilwiya teaching hospital, Al-Yarmouk hospital, Medical city department of teaching laboratories, and from four private laboratories. The patients mean age was 43.1 years with a range of 20 to85 years. The remaining 10 normal cervical postmortem tissue biopsies were obtained from the institute of forensic medicine and considered as control group. These autopsies were taken from virgin female cervices, their mean age 23.1 years with a range of 18-30 years. In Situ Hybridization was performed for the detection of HPV on cervical tissue.Results: All normal control cases showed no specific signals for HPV DNA. 6 (30%) of 20 cases of cervical tissue with codylomatous changes, 1 (11.11%) of 9 cases of CIN I.3 (21.43%) of 14 cases of CIN II/III, and 9 (33.33%) of 27 cases of ISCC were shown to be positive for HPV 6/11/16/18/31/33 DNA.Conclusion: The In situ hybridization enabling direct visualization of viral tissue distribution and better substantiate HPV as a causal agent in cervical neoplasia.A significant association (p <0.05) was found between Insitu Hybridization signal pattern and the histological type of cervical noeplasia.

خلفية الدراسة: أظهرت الدراسات السريرية و الإحصائية أن الفايروس ألحليمي البشري يلعب دورا كبيرا في تطور مختلف أنواع إصابات عنق الرحم لذلك اعتبر عامل ملوث أساسي مسبب للإصابات السرطانية في عنق الرحم.هدف الدراسة: لتحديد تفشي الفايروس ألحليمي البشريDNA باستخدام تقانة التهجين الموضعي في نماذج الأنسجة الأرشيفية المطمورة بشمع البارافين المأخوذة من إصابات عنق الرحم و مقارنتها بخزعات ما بعد الوفاة المأخوذة من أعناق رحم طبيعية.طرق العمل: تضمن البحث ثمانين عينة و كان سبعون منها مأخوذة من الأنسجة الأرشيفية المطمورة في شمع البارافين و التي تمثل مجموعة الخطر المحتوية على دلائل تشير إلى وجود إصابة بالفايروس الحليمي البشري مع أو وجود حالات سوء نمو ظهارية (السرطانية أو غير السرطانية ) و قد اختيرت العينات من ملفات مختبرات التحليلات النسيجية للمدة بين 1998 و لغاية 2005 من مستشفى الكاظمية التعليمي, مستشفى العلوية,مستشفى اليرموك التعليمي, المختبرات التعليمية في مستشفى مدينة الطب و بعض المختبرات الخاصة.كان متوسط عمر المرضى 43.1 سنة ضمن مدى يتراوح بين 20-85 سنة.أما العشر عينات المتبقية تمثل خزع بعد الوفاة من عنق أرحام نسوة أبكار كانت أعمارهم تتراوح بين 18-30 سنة و بمعدل عمر23.1 كمجموعة سيطرة اثبت الفحص النسيجي عدم وجود عوارض أو إصابات فيها.لقد تم التحري بواسطة تقانة التهجين الموضعي عن DNA هذا الفايروس في أنسجة عنق الرحم.النتائج: لقد أظهرت الدراسة انتشار موجبيه DNA هذا الفيروس في مجموعة الخطرهي30%,11.11%,21.43%,33.33% في كل العينات التي تحمل في ثناياها دلائل على الإصابة بالفايروس الحليمي البشري,حالات سوء النمو الظهارية البسيطة ,حالات سوء النمو الظهارية المتوسطة و الشديدة و أخيرا حالات سرطان عنق الرحم المحرشف, على التوالي , في حين لوحظ عدم وجود DNA الفايروس في مجموعة السيطرة.الاستنتاج: إن تقانة التهجين الموضعي تجسد القيمة الحقيقية لوجود الفايروس كمسبب للمرض ووجد إن هناك ترابط وثيق بين توزيع علامات التهجين الموضعي والنوع النسيجي لسرطان عنق الرحم.

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