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Article
HLA Class I and Class II Polymorphisms and Anti-nuclear Antibodies in Hyperprolactinaemic Iraqi Females with Primary Infertility

Author: Ali H. Ad'hiah
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: PISSN: 00419419 / EISSN: 24108057 Year: 2008 Volume: 50 Issue: 4 Pages: 475-479
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: The study was conducted to investigate the association between hyperprolactinaemia and markers of human leukocyte antigen (HLA) system in a sample of Iraqi infertile females, together with the profile anti-nuclear antibodies (ANA). Objectives: One hundred and seventy five female patients (age range: 20 -40 years) were recruited in this study. They were attending the Institute for Embryo Research and Infertility Treatment (Al-Nahrain University) during the period January 2005 - September 2006. Results:After clinical and laboratry evaluations, it was found that 100 patients were hyperprolactinaemic, whereas the other 75 patients were euprolactinaemic, therefore, they were considered as a control group. Based on serum level of prolactin (22-29, 30-39 and ≥ 40 ng/ml), the total hyperprolactinaemic patients were divided into three groups; I (35 patients), II (40 patients) and III (25 patients), respectively. The HLA antigens showed significant variations between patients (total and groups) and controls. In total patients, B8 (25.0 vs. 9.3%), DR3 (48.0 vs. 17.3%) and DR4 (39.0 vs. 13.3%) showed significant increased frequencies, while B35 showed a significant decreased frequency (7.0 vs. 24%). The latter decrease was also observed (5.7 vs. 24.0%) in group I of patients, which also showed a significant increased frequency of DR3 (54.3 vs. 17.3%). In groups II and III of patients, only DR3 (45.0 and 56.0, respectively vs. 17.3%) and DR4 (37.5 and 56.0, respectively vs. 13.3%) showed significant increased frequencies. Autoantibody evaluation by ANA test revealed that 22% of the total patients was positive, while all control subjects were negative, and such positivity paralleled the increased level of serum prolactin.


Article
The possible Association of HLA Class II with Bladder Cancer in Iraqi Patients

Author: Shatha M.J.Al-Khateeb
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: PISSN: 00419419 / EISSN: 24108057 Year: 2007 Volume: 49 Issue: 4 Pages: 434-437
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: - Genetic Factors have a major role in the development of bladder cancer. Objectives: - This study was carried out to shed a light on the possible association of HLA class II antigens and BC patients and to correlate this finding with the family history. Patients and Methodes :- Lymphocytotxicity assay had been used to assess HLA- typing of 65 BC patients and 50 healthy controls. Results:- comparison between BC patients and healthy controls showed several antigens deviations in their frequencies. HLA-DR1, HLA-DQ1 and HLA-DQ3 antigens were observed with increased frequencies in patients group with significant differences (P=0.000, 0.000 and 0.017 respectively). Moreover there was decrease frequency of HLA-DR7 in patients group (P=0.010). Stastical analysis showed non significant correlation of the specific HLA –Ags with family history. Conclusions: - This finding demonstrated that HLA-DR1, DR7, DQ1 and DQ3 might play a role in BC susceptibility. • Department of clinical biochemistry. Collage of Medicine Al-Mustansyriah University • Department of Microbiology. Collage of Medicine. Baghdad University • Ministry of Health.


Article
HLA-DRB ALLELS, IL-10 AND VITAMIN D LEVEL: POTENTIAL IMPACT ON MULTIPLE SCLEROSIS

Authors: Ahmed A.H. Abbas احمد عبد الحسن عباس --- Alaa H. Khaliel علاء حسن خليل --- Nawfal M. Shaheed نوفل ماضي شهيد
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2016 Volume: 14 Issue: 2 Pages: 159-165
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background:Multiple sclerosis (MS) is a chronic inflammatory autoimmune disease with unknown etiology. Variation in the HLA-DRB1 gene is the potent genetic risk factor for promoting MS. Other agents relate with an increased risk of developing MS include the cytokines levels such as interleukin-10 (IL-10) and the vitamin D (vit. D) deficiency.Objectives:To study a possible role of HLA-DRB1, vit. D deficiency as a risk factor for MS development and to estimate the level of IL-10 in the serum of MS patients and its role in disease initiation or progression.Methods:Sixty MS patients, of them 30 were newly diagnosed with an age range between 13 and 58 years were included in this study, in addition to thirty healthy volunteers their gender and age matched with patients group serve as a control group. Blood samples collected to assess serum levels of vit. D and IL-10 by Enzyme-Linked Immunosorbant Assay (ELISA) and for DNA extraction, which used in the HLA-DRB1 2 digit genotyping.Results :The HLA-DRB1 genotyping revealed that the HLA-DRB1*15 frequency was higher but statistically insignificant in the MS patients as compared with healthy control group. IL-10 level was significantly lower in MS patients on treatment than the control group. On the other hand, vit. D in the newly diagnosed MS patients was significantly different from the control group (higher in the control group), but there was no variance with MS patients on treatment, the level of vit. D in the studied groups was less than global value.Conclusion:In MS patients the frequency of HLA-DRB1*15 was higher than control group but the difference was not significant. In addition, the level of IL-10 and vit. D may have a role in the development of MS.Keywords:Multiple sclerosis, human leukocyte antigen, IL-10


Article
Study of HLA-class II Serotyping and cellular immunity CD4+T, CD8+T cells in Iraqi patients with Rheumatoid arthritis
دراسة التنميط المصلي لكريات الدم البيضاء و المناعة الخلوية للخلايا التائية المساعدة والسمية لدى المرضى العراقيين المصابين بالتهاب المفاصل الرثوي

Authors: Hanaa N. Abdulla هناء ناجي عبدالله --- Amina N. Al-Thuwan امنه نعمة الثويني --- Mohammed Ibrahim Nadir محمد ابراهين نادر
Journal: Al-Nisour Journal for Medical Sciences مجلة النسور للعلوم الطبية ISSN: 26640562 26640554 Year: 2019 Volume: 1 Issue: 1 Pages: 98-107
Publisher: Al-Nisour University College كلية النسور الجامعة

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Abstract

Rheumatoid arthritis is a complex polygenic disease whose environmental and genetic factors contribute to both of the predisposition and developing of disease. The current study was conducted to determine the frequency of human leukocyte antigen (HLA-DR, -DQ) and correlate to this variation with disease severity and detected on the role of cellular immune response in pathogenesis of rheumatoid arthritis. Fifty blood samples were collected from RA patients and 50 healthy control group with no history of inflammatory arthritis. The HLA class II (DR, DQ) estimated serologically by using micro lymphocytotoxicity test. The result indicated that a highly significant frequencies of HLA-DR4 and DR53 antigens were observed in RA as compared to healthy group (P<0.001). Our results revealed that there are significant higher frequencies of HLA-DQ3 antigen (P<0.001) in RA group as compared to control groups. Additionally, the percentage of CD8+ has been decreased in patients in comparison with healthy group, whereas the percentage of CD4+ T-cell has been slightly increased in patients in comparison with healthy control group with nonsignificant differences. The results of this study proved that nonsignificant correlation which was observed between cellular immune response (CD4 and CD8) among patients.

يعد التهاب المفاصل الرثوي مرض اضطراب مناعي ذاتي وتساهم العوامل البيئية والوراثية مساهمة شديدة في الاستعداد للإصابة بالمرض. اجريت الدراسة الحالية للتحري عن مستضدات الكرية البيضاء البشري النمط الثاني( HLA ,وعلاقتها في اختلاف شدة المرض والتحري عن دور الاستجابة المناعية الخلوية في Class-II- HLA-DR ,-DQ) عينة من الاشخاص 50 عينة دم من مرضى التهاب المفاصل الرثوي و 50 التسبب في التهاب المفاصل الرثوي. جمعت الاصحاء ظاهريا. تم قياس تنميط مستضدات الكريات البيضاء مصليا( ( باستخدام فحص سمية الخلايا HLA-DR,-DQ ). وأظهرت النتائج بأن هنالك زيادة معنوية في تكرار مستضدات الكريات البيضاء Lymphocytotoxicity اللمفاوية ( HLA DR4البشرية ( ( -) لدى مرضى التهاب المفاصل الرثوي مقارنة بالأصحاء DR53,HLA. فضلا عن P<0.001) وجود زيادة في تكرار مستضدات الكريات البيضاء البشرية (وبفارق عالي المعنوية في مرضى التهاب HLA-DQ3) المفاصل الرثوي مقارنة بالأصحاء( CD8+T . بالإضافة الى ذلك, انخفاض نسبة الخلايا التائية السمية ( P<0.001) ) لدى مرضى التهاب المفاصل الرثوي مقارنة مع مجموعة الأصحاء ، بينما اظهرت النتائج ارتفاعا في نسبة الخلايا cells CD4+Tcell التائية المساعدة ( ) لدى المرضى مقارنة بمجموعة الأصحاء. تؤكد نتائج الدراسة الحالية عدم وجود علاقة s معنوية بين الخلايا( .(CD4+T and CD8+T cells


Article
Expression of CD45, CD34, CD10, and human leukocyte antigen‑DR in acute lymphoblastic leukemia

Authors: Faris H. Jaafar --- Areej Emad Kadhom
Journal: Iraq Joural of Hematology المجلة العراقية لامراض الدم ISSN: 20728069/25432702 Year: 2018 Volume: 7 Issue: 1 Pages: 14-19
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

BACKGROUND: Immunophenotypic analysis of leukemic cells by multicolor flow cytometry usingdifferent monoclonal antibodies labeled to various flourochromes, is an important, precise and rapidinvestigation for diagnosis, classification, prognosis prediction, and minimal residual disease detectionin acute lymphoblastic leukemia (ALL).OBJECTIVES: The aims of study were to study the expression of CD45, CD34, CD10, humanleukocyte antigen‑DR (HLA‑DR) in B and T‑ALL among 114 Iraqi patients and compare findingswith other reports worldwide.PATIENTS, MATERIALS AND METHODS: A retrospective cross‑sectional study was conductedon 114 ALL patients of various age groups from different hematology centers in Baghdad who werereferred to Flowcytometry Department at the Bone Marrow Transplantation Center/Private NursingHome Hospital/Medical City for immunophenotypic classification using multi‑color flow cytometryfrom the January 1, 2016, to the August 31, 2016.RESULTS: Out of 114 patients, 71 patients were pediatric patients and 43 were adults. Eighty‑threepatients were classified as B‑cell ALL and 31 patients as T‑cell ALL. There was significant associationbetween male gender, high white blood cells count and T‑cell ALL subtype. Negative‑dim‑moderateCD45, positive CD34, and positive HLA‑DR expressions were significantly associated with B‑cellALL. Common ALL antigen was seen in 86.7% of the B‑cell ALL patients and was accompaniedcTdT expression. Aberrant myeloid antigens were observed in 22.9% of B‑cell ALL patients and in35.5% of T‑cell ALL. About 4.8% of the patients with B‑cell ALL had aberrant T‑linage antigens while6.5% of the patients with T‑cell ALL expressed aberrant B‑cell lineage antigens.CONCLUSION: Immunophenotypic expression of ALL cells among Iraqi patients is to somewhat inaccordance with various researches worldwide making immunophenotyping flow cytometry a crucialappliance in diagnosis, classification, risk stratification, and minimal residual disease detection in ALL


Article
ASSOCIATION OF CELIAC DISEASE WITH HLA-DRB1 AND HLA-DQB1 ALLELES IN A SAMPLE OF IRAQI PATIENTS
علاقة مرض حساسية الحنطة بالنمط الوراثي لمستضد كريات الدم البيضاء الصنف الثاني في عينة من المرضى العراقيين

Author: Hanaa N. Abdullah
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2012 Volume: 11 Issue: 2 Pages: 529-536
Publisher: Baghdad University جامعة بغداد

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Abstract

Celiac disease (CD) is a complex disorder triggered by gluten affecting genetically predisposed individuals. The CD is triggered by the binding of one or more gliadin peptides to CD associated HLA class II molecules. Fifty patients with CD and fifty control group were studied. The sera were qualitativelymeasured for anti-TTG-IgA,IgG antibodies and anti-gliadin- IgA,IgG antibodies by ELISA method. The HLA class II (DRB1, DQB1) were genotyped by using Polymerase Chain Reaction-Sequence Specific Primers (PCR-SSP). In the current studypositivity for Anti-TTG antibodies showed a frequency of 38% in CD patients as compared with the control group 0.0%, while high frequency of Anti- gliadin antibodies positivity in celiac disease patient's sera showed 22% as compared with the control group 0.0%with a highlysignificant difference were highly (P=0.001).Human leukocyte antigen genotyping revealed that the DR-alleles, DRB1*03(01,06,08,10), DRB1*0701 and DQB1*02 (01,02) showed highly significant increased frequency in CD as compared withthe controls, while the DRB1*1302 and DQB1*0601 alleles showed significant decreased frequency in CD when compared with the control groups.

إن مرض حساسية الحنطة يحفز بواسطة الكلوتين الذي يتأثر بالإستعداد الوراثي للبشر والتأثيرات البيئية, إذ أن مرض حساسية الحنطة يحفز عن طريق إرتباط واحد أو أكثر من ببتيدات الكلوتين بالمرض والمتعلق بمستضدات الكريات الدم البيضاء الصنف الثاني. تم دراسة خمسون عينة من مرضى حساسية الحنطة ومثلها من مجموعة السيطرة. تم قياسAnti- gliadin antibody , Anti-TGG antibodies في مصول المرضى ومجموعة السيطرة بطريقة اليزا. تم تحديد الأنماط الوراثية لمُستضد الكريات الدم البيضاء الصنف الثاني بتقنيـة البادئ المناوع لتعاقب سلسلة تفاعل البلمرة (PCR- SSP). إن إيجابية فحص (Anti-gliadin antibody) بلغت 22% لمرضى حساسية الحنطة مقارنة بفحص(Anti-TGG antibodies) والذي بلغت ايجابية الفحص 38% مقارنة بمجموعة الأصحاء مع فارق عالي المعنوية(P=0.001). أظهر التنميط الجيني بأن الأليلات03(01,06,08,10), DR *0701 , DQB1*02 (01,02) مرتبطان بمرض حساسية الحنطة وبفارق معنوي عالي مقارنة بمجموعة الأصحاء (Pc=0.0001) ، في حين أظهر الأليلانDRB1*1302 , DQB1*0601 تكرار واطئ المعنوية في مرضى حساسية الحنطة مقارنة بمجموعة الأصحاء (Pc=0.0001).

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