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Article
Study of some biological characteristics of hydatid cysts in sheep in al-Shuula~area in Baghdad
دراسة بعض الصفات البايولوجية للأكياس العدرية للأغنام المذبوحة في منطقة الشعلة/بغداد

Author: KHALIDA HUSSAIN YOUNIS خالدة حسين يونس
Journal: The Iraqi Journal of Veterinary Medicine المجلة الطبية البيطرية العراقية ISSN: 16095693 Year: 2011 Volume: 35 Issue: 1 Pages: 180-189
Publisher: Baghdad University جامعة بغداد

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Abstract

The study was implemented to identify some the biological characteristics of 2428 hydatid cysts in 353 naturally infected animals out of 2781 slaughtered sheep in al-Shuula~area in Baghdad. The results revealed that 1669 cysts in 211 infected females and 759 cysts were presence 142infected males. The diameter of hydatid cysts varies from 0.1-16.4 cm . The surrounding cysts were larger than the cysts in the center of the infected organ with multiple cysts. The cysts were unilocular rounded. and mostly found in infected livers. They were few cysts with free fertile or sterile daughter cysts. The hydatid liquid was clear for most hydatid cysts. and its volume was ranged between (0.2-930) ml. The protoscolises were found white in color and their number were ranged between 4322.8±127.8 in male`s livers and9472.5 ±432.5 in female`s livers. The bio-activity of hydatid cysts reached with an average 73.32% and 89.3% in livers . The fertility percentage of the cysts was 60.25 %. and it was higher in females than in males. The study also revealed that such percentage in infected livers was higher than that of infected lungs and that of the second aged group ( two _ less than four years ) for both animals sexes . The results of the present study indicate no relation among diameter of hydatid cysts. quantity of protoscolises inside the cysts. fertility and viability of protoscolises.

تمت دراسة بعض الصفات البايولوجية ل2428 كيس عدري متطفل في أكباد ورئات 353 حيوأن مصاب طبيعيا من مجموع 2781 حيوأن مذبوح و مفحوص في منطقة الشعلة/بغداد وأظهرت النتائج وجود 1669 كيس في 211 أنثى مصابة و759كيس في142 ذكر مصاب. لقد تباينت قياسات أقطار الأكياس العدرية من 0.1- 16.4 سم . وأن الأكياس العدرية المحيطية الموقع اكبر من الأكياس العدرية المركزية الموقع في العضو المصاب بالعديد منها. جميع الأكياس العدرية كأنت من النوع وحيد الفجوة unilocular cyst كروية الشكل أغلبها في الأكباد المصابة . مع وجودالقليل من الأكياس البنيوية الحرة كأن بعضها خصبا وبعضها عقيما . أما السائل العدري فقد كأن رائقا لمعظم الأكياس العدرية وتراوح حجمه بمدى 0.2 -9300 مليليتر.وتبين أن لون الرؤيسات العدرية أبيض. أما أعدادها فقد بلغت في أكباد الإناث 9472.5 ±432.5 أعلى مما هي عليه في أكباد الذكور 4322.8 ± 127.8 وبلغت حيويتها بمعدل 73.32%وبنسبة89.3% في الأكباد لوحدها . سجلت نسبة خصوبة 60.25% وكأنت في الأكياس العدرية للإناث أعلى مما هي عليه في الذكور. وأظهرت النتائج أعلى نسبة للأكياس العدرية الخصبة في الكبد أعلى مما هي عليه في الأكياس العدرية الرئوية وفي الأكياس العدرية للفئة العمرية الثأنية للإناث ( سنتين – أصغر من أربع سنوات) . كشفت نتائج البحث ايضا عدم وجود علاقة بين قطر الكيس العدري وخصوبته وعدد الرؤيسات التي يحتويها وحيويتها.

Keywords

Echinococcus --- Hydatidosis


Article
Hepatic Hydatidosis , Analysis Of 126 Cases

Author: Dr. Abdulhadi Jassim Tlefih
Journal: Karbala Journal of Medicine مجلة كربلاء الطبية ISSN: 19905483 Year: 2007 Volume: 1 no.2 Issue: 1 Pages: 150-157
Publisher: Kerbala University جامعة كربلاء

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Abstract

Background: Hydatid disease ( H D) is a serious health problem in endemic areas, asin our country. It can affect any organ in the body especially the liver. Surgery is stillthe procedure of choice in most cases of H D, despite other methods of treatmentavailable.Objectives : The aim of this study is to know the prevalence of hepatic hydatidosis(H H) , and to study presentation , types of treatment, surgical procedures andcomplications.Methodes: 126 patients with H H were treated in Hammad Shihab military hospital in5 years period ( from July 1997 to June 2002 ) . 122 patients were treated surgically.Results: (74.6%) of patients hailed from rural area. Most of the patients were female,with male to female ratio (M : F) was 1 : 2 . ( 32.53%) of patients were between 31and 40 years and the mean age was ( 35.46 ) years .(75.3%) of patients presented with abdominal pain or discomfort . The disease wasdiscovered incidentally in (17.46%) . Isolated liver was involved in ( 66.7 % ). Theright lobe of the liver was affected in ( 80.95 % ) . ( 67.4 % ) of cases had singlehydatid cyst ( H C ) . Synchronous pulmonary hydatid cyst (P H C) occurred in(7.9%) of cases.Post operative complications ocurred in ( 3.2 % ) included (1.6%) developedrecurrent hepatic hydatid cyst (H H C ) , (0.8%) had subphrenic abscess and (0.8%)experienced biliary cutaneous fistula .Conclusion: The right lobe of the liver was the commonest location of H H C . Thecommonest presentation was abdominal pain or discomfort. All patients with HHCshould be investigated for synchronous P H C.


Article
Association of HLA class I -A and B alleles with hydatidosis In Iraqi patients

Author: Samir Sabaa1,Lazim Hammed2,Batool Al-Ghurabi3
Journal: Almuthanna Journal of Pure Science (MJPS) مجلة المثنى للعلوم الصرفة ISSN: 22263284 Year: 2014 Volume: 2 Issue: 1
Publisher: Al-Muthanna University جامعة المثنى

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Abstract

Hydatidosis is a parasitic disease caused by infectionwith larval stage of cestode worm Echinococcusgranulosus ,it is a worle_wide disease results in serioushealth and financial loss. Iraq considered as an endemicregion for this disease. the association of HLA –allelesfrequency with the occurrence of various diseases werestudied . and significant associations were found, nosufficient studies on hydatidosis and possible role of HLAtyping and the immune status of the patients andassociation of them with cyst characteristics ,andcytokines profile and their effect on production ofimmunoglobulin . blood samples were collected forextraction of DNA and amplification by (PCR-SSO) andHLA typing by Automatic Line Probe Assay (Auto-Lipa) forHLA typing of both patients and healthy control groups, tostudy the association of certain HLA alleles. Withoccurrence of Hydatid disease. The present studyreported increase in frequency of HLA-A*0273 in hydatidpatients compare to healthy control .

Keywords

HLA --- alleles --- Hydatidosis --- PCR


Article
Prevalence of hydatid cyst in human and animals in Sulaimaniyacity and Saedsadq distract

Authors: Hersh A. Amin , --- Lazem H. Al-taie , --- Mohamad O.Mohamad ,
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2008 Volume: 50 Issue: 2 Pages: 175-183
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Hydatid disease is widespread and considered endemic in the Middle
East and the Mediterranean, Iraq is one of the countries with a high endemicity of this
disease.
Objectives: The aim of this study is to diagnose hydatidosis and to identify the
prevalence in human and animals in two different areas Sulaimaniya governorate
which include Sulaimaniya city and Saedsadq district.,
Methods: Seroepidemiological survey was conducted by using enzyme-linked
immunosorbent assay (ELISA) and Indirect Haemagglutination IHA to detect anti
Echinococcus granulosus antibody in random blood samples (536) of different sex,
ages, and occupation out patients, in Sulaimaniya and Saedsadq. In the animal study
inspection and examination of the internal organs (liver, lungs, spleen and heart)
Results: The Seropositivty in Saedsadq was higher( 3.7% )than in Sulaimaniya(
2.5%), and in female (2.8%, 6.4%) were higher than in male (1.6%, 1.2%)
respectively both in Sulaimaniya and Saedsadq. In animal study results revealed that
In Sulaimaniya infection rates were (1.5% of sheep, 0.5% of goats and 0.2% of
cattle), less than in Saedsadq (7.5%, 2.8% and 1.7%) respectively,
Conclusion: The seropositive was higher in Saedsadq than Sulaimaniya , and higher
among females than males in all age groups. The prevalence and fertility of hydatid
cysts in sheep is higher than goat and cattle, sheep. Liver is the main involved organ.


Article
Some serological tests In diagnosing cystic hydatidosis
بعض الفحوصات المناعية في تشخيص داء الاكياس العذرية

Authors: Mahmood, M. Atia --- Ismail A. Al-Hadithi --- Mahmood Al-Mahdawi, Mina S. Al-Jeboori
Journal: Al- Anbar Medical Journal مجلة الأنبار الطبية ISSN: PISSN: 27066207 / EISSN: 26643154 Year: 2005 Volume: 5 Issue: 1 Pages: 61-64
Publisher: University of Anbar جامعة الانبار

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Abstract

Background : Cystic hydatidosis (CH) caused by Echinococcus granulosus is an important zoonosis world wide . several test are currently used for screening and for diagnosis . Objective : To show the possibility of diagnosis via the presence of specific antibody for CH in serum of patients infected with this disease .Methods: Passive hemagglutination test (PHA), double immunodiffusion test(DD5) and counter immunelectrophoresis (CIEP) were used for diagnosis .Results: The results showed that the antigen of whole HC has a high antibody titer than filtrate and protoscoleses . PHA test gave sensitivity (83%) when the whole antigen used. And gave from 66-80% when protoscoleses antigen was used while 50-70% was detected when the filtrate was used, and CIEP gave a high sensitivity than DD5 .Conclusion : PHA test a higher diagnostic sensitivity than CIEP and DD5 test .Key words : Hydatidosis , Double immunodiffusion test, Immunelectrophoresis

الخلاصة : الخلفية: يعد مرض الاكياس المائية العذرية المنتشر عالمياً من اهم الامراض المشتركة بين الانسان والحيوان وتستخدم العديد من الاختبارات لتشخيص الاصابة به .الهدف : معرفة امكانية تشخيص داء الاكياس العذرية من خلال وجود مستضدات الكيس العذري .طرق العمل: استعملت في هذه الدراسة اختبارات التلازن الدموي غير المباشر والانتشار المناعي المزدوج والترحيل الكهربائي .النتائج: كان لمستضد سائل الكيس العذري استجابة مصلية اعلى (1:256) من مستضد الراشح والرؤيسات . واظهر اختبار التلازن الدموي الغير مباشر حساسية عالية عند تشخيص الاصابة المصلية بمستضد الكيس الكلي (83%) . اما مستضدات الرؤيسات فقد وصلت الى 66.6 -80% في حين وصل مستضد الراشح الى 50-70%.الاستنتاج: تبين ان اختبار الهجرة الكهربائية ذا تحسس اعلى من الاختبار المناعي المزدوج


Article
Prevalence and molecular studies on Echinococcus equinus isolated from necropsied donkeys
دراسة عن الإنتشار والتشخيص الجزيئي لطفيل Echinococcus equinus المعزول من الحمير المشرحة

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Abstract

In the present study, forty donkeys of different ages and sexes at Giza Zoo, Egypt were investigated between October 2015 and September 2016 for the presence of hydatidosis disease. Hydatid cysts were detected in the livers of 10% of the examined donkeys and these cysts had a fertility rate 100%. Female donkeys were infected with cysts more than males and all infected donkeys were old aged with no cases of infection were detected in young or adult donkeys. Using molecular tools, the DNA extracted from cysts that had been isolated was subjected to PCR amplification, using synthesized oligonucleotide primers, and these were constructed to target the 299 bp within the (ND2) gene, which is considered to be specific for the Echinococcus equinus genotype. The sequenced PCR products showed homology to E.equinus (G4 or horse strain genotype). These results can be used in future to pursue the epidemiological status of the causative strain of hydatidosis in equines at the study area.

في هذه الدراسة، تم فحص عدد أربعين حماراً من مختلف الأعمار والأجناس في حديقة حيوانات الجيزة، مصر في الفترة بين أكتوبر 2015 وسبتمبر 2016 لوجود مرض الأكياس المائية. تم الكشف عن الأكياس المائية لمرض الأكياس المائية في كبد 10٪ من الحمير المفحوصة و كان معدل الخصوبة لتلك الأكياس 100٪. أظهرت الحمير الإناث معدل إصابة أعلى من الذكور وكانت جميع الحمير المصابة من الأكبر سناٌ ولم يتم الكشف عن أي حالات إصابة في الحمير الصغيرة أو البالغة. باستخدام أدوات التشخيص الجزيئية، تم إخضاع الحامض النووي الديوكسي ريبوزي المستخرج من الأكياس المعزولة لتفاعل إنزيم البلمرة المتسلسل باستخدام بوادئ تفاعل مخصصة لتضخيم 299 زوج قاعدي بداخل جين ND2 والخاصة بعترة Echinococcus equinus و بإجراء إختبار التتابع الجيني اظهرت العينات المعزولة تطابقا لعترة Echinococcus equinus. يمكن استخدام هذه النتائج في المستقبل لمتابعة الحالة الوبائية للسلالة المسببية لمرض الأكياس المائية في الخيول بمنطقة الدراسة.

Keywords

hydatidosis --- PCR --- horse strain --- equines


Article
In Vitro Evaluation of the Scolicidal Activity of Cyclosporin A against the Protoscolices of Human Echinococcus Granulosus

Author: Samer Noori Hashim
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2007 Volume: 6 Issue: 4 Pages: 356-561
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGROUND :Hydatid disease, also known as echinococcosis or hydatidosis, is caused by infection with larva of Echinococcus granulosus, which causes cystic hydatid disease. Surgery is the recommended treatment for hydatid cysts; however, drug therapy and percutaneous drainage have recently been introduced as alternative treatments. The scolicidal agents, including 3% hydrogen peroxide, 10% chlorhexidine, 20% hypertonic saline have been used mainly during surgical manipulation of the cysts. Recently cyclosporin A has been found to be lethal for E. granulosus protoscolices in vitro using cultured sheep hydatid cyst.METHOD:The present work was designed to evaluate the effectiveness of different concentrations of cyclosporin A as a scolicidal agent on protoscolices of Echinococcus granulosus in vitro. Hydatid cysts were collected from 42 patients (10 males and 32 females with age range from 12–61 years) that have hydatosis during the surgical operation for the removal of these cysts. Eeosin exclusion test was utilized to examine the scolicidal activity of different concentrations of cyclosporin A compared to different concentrations of hydrogen peroxide, in addition to the effects on the integrity of the germinal layer compared to that produced by sodium hypochlorite.RESULTS AND DISCUSSION:The results indicated that cyclosporin A, when used in a concentration of 25 μg/ml, is more effective and safer than 3% solution of hydrogen peroxide; it can be used with sodium hypochlorite for complete scolicidal effect and melting of the germinal layer. In conclusion, cyclosporin A can be a good candidate as a scolicidal agent during surgical removal of hydatid cysts.CONCLUSION:The present work indicated that cyclosporin A has a good scolicidal effect when used in a concentration of 25 μg/ml, and it is more effective and safer than 3% solution of hydrogen peroxide, it can be used in combination with sodium hypochlorite for complete scolicidal effect and melting of the germinal layer of the hydatid cyst. This may give an opportunity for using this compound in percutaneous drainage


Article
Cardiac hydatidosis : A rare infection of the heart with hydatid cyst of Echinococcus granulosus can produced anaemia
داء العدريات القلبي : اصابة نادرة بالكيس العدري لطفيلي المشوكة الحبيبية E. granulosus تؤدي الى الاصابة بفقر الدم

Author: Amal Kh. Khalaf امــــل خضير خلف
Journal: JOURNAL OF THI-QAR SCIENCE مجلة علوم ذي قار ISSN: 19918690 Year: 2014 Volume: 4 Issue: 3 Pages: 44-47
Publisher: Thi-Qar University جامعة ذي قار

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Abstract

Rarely , heart was infected with hydatid cyst of E. granulosus , where the current study were recorded only one infection of heart with hydatid cyst among 225 male of mice experimentally infected with hydatid cyst leading to produced anaemia. Histological examination of hydatid cyst components was explained presence of three layers include fibrous layer, laminated layer, and germinal layer or endocyst. So there is a granulomatous fibrous tissue in the pericardium.

تعتبر اصابة القلب بالكيس العدري الطفيلي المشوكة الحبيبية من الاصابات النادرة , اذ سجلت الدراسة الحالية اصابة واحدة فقط من بين 225 من ذكور الفئران المصابة تجريبيا وقد نتج عن الاصابة فقر الدم او الانيميا . وعند الفحص النسيجي لمكونات الكيس المائي بعد ستة اشهر من الاصابة لوحظ وجود طبقات الكيس الثلاثة كاملة وهي الطبقة الليفية والطبقة الصفائحية والطبقة المولدة او الداخلية للكيس . كما لوحظ تليف حبيبي وتجمع للخلايا في منطقة امام القلب .


Article
Human Hydatidosis in Duhok – Kurdistan Region – North of Iraq

Authors: Adel Talib Mohammed Al _Saeed --- Khoshi Salim Abdulhafith Almufty
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2016 Volume: 13 Issue: 1 Pages: 125-133
Publisher: Babylon University جامعة بابل

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Abstract

Forty eight sera were collected from patients with suspected Hydatid cyst (HC) admitted to Duhok public and private hospitals and from apparently hydatid cyst free people during the period from 1/ October 2010 to 30 / April 2011. All sera were examined for the presence of specific antihydatid antibodies by ELISA IgG (DRG kit - Germany) and Indirect Hemagglutination test (IHA). The liver was the most common site of HC in 26 (54.2%) of cases followed by the lung HC in 12 (25.0%) of cases. Regarding the residency of examined patients, most of the HC cases were found to be from rural or semi-rural areas such as Sinjar 17 (35.4%), Sumel 11 (22.9%), Talfer 6 (12.5%), Telkif 5 (10.4%) and Shekhan 2 (4.2%) of cases.The HC cases reported from patients of Duhok and Zakho were 4 (8.3%) and 3 (6.2%) respectively. The highest rate of infection was among housewives 25 (52.1%) followed by free workers 12 (25.9%) and the lowest rates were recorded from the government employed, children and students groups. The highest rate was found in age groups 11-20 and 21-30 years at a rate of 11(22.9%) for each from the total number of cases followed by the age the group 31-40 years at a rate of 10(20.8%) of the cases. The highest percentage was among females 31(64.4%) of the cases. Out of 48 patients, it was found that 34 (70.8%) had a history of contact with dogs and animals, and 14 (29.1%) did not have any contact with dogs and animals.


Article
Detection of Cystic Echinococcosis Antigen Concentration in Serum Patients
الكشف عن تركيز مستضد داء المشوكات الكيسي في مصل المرضى

Authors: Waheeda Rashid Ali وحيدة رشيد علي --- Lamyaa Ali Hussain لمياء علي حسين --- Ali Abdul Hussain Mahdy علي عبد الحسين مهدي
Journal: Diyala Journal For Pure Science مجلة ديالى للعلوم الصرفة ISSN: 83732222 25189255 Year: 2016 Volume: 12 Issue: 3 - part 1 Pages: 122-134
Publisher: Diyala University جامعة ديالى

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Abstract

Cystic Echinococcosis (CE) or Hydatidosis, a parasitic disease that caused by larvae of Echinococcus granulosus, is an important zoonosis endemic in large parts of the world and Iraq. Diagnosis of hydatidosis antibodies is based on immunodiagnostic methods (ELISA), along with radiological and ultrasound examinations, when treatment by chemotherapy and surgery.The objectives of the present study were to determine a specific antigen concentration -based ELISA technique for diagnosis of hydatidosis and compare it before and after surgery A total sample of 102 Iraqi subject from different hospitals were included in this study. They were distributed as 52 patients (43 females and 9 males, included the same group of patients study after 3-7 days surgery to remove hydatid cyst), and 50 persons healthy controls. Hyper immune-serum was raised against hydatid cyst fluid in rabbits. Anti-hydatid cyst IgG was purified by Ione exchange chromatography. The results show that increased circulating hydatid cyst antigen concentration before surgery 38.7μg/ml than antigen concentration after surgical removal hydatid cyst 25.7 μg/ml the difference was significant at P≤0.01. In the present study, the presence of circulating antigens in sera of hydatidosis patients by indirect ELISA[new kit] was detected hydatid cyst antigen in preoperative sera in 50 out of 52 (96.15%), the test demonstrated antigen in 48 out of 52(92.30%) postoperative sera.

داء المشوكات الكيسي او داء الاكياس العدرية, مرض طفيلي يتسبب عن الطور اليرقي للمشوكة الحبيبية, وهو من الامراض المهمة المشتركة بين الانسان والحيوان, يتوطن في اجزاء كبيرة من العالم والعراق. تشخيص الاجسام المضادة لداء المشوكات يعتمد على طرائق التشخيص المناعية (الاليزا) مع الفحوصات الشعاعية والموجات فوق الصوتية (السونار), بينما يعالج بالعلاج الكيميائي والجراحة. الهدف من هذه الدراسة لتحديد تراكيز المستضدات الخاصة بالاعتماد على تقنية الاليزا لتشخيص داء المشوكات والمقارنة قبل وبعد الجراحة, تضمنت الدراسة مامجموعه 102 عينة عراقية من مختلف المستشفيات, موزعة 52 مريض (43 اناث و 9 ذكور قبل الجراحة, نفس المجموعة السابقة درست بعد 7-3 أيام من ازالة الاكياس العدرية جراحيا), و 50 شخصا من الاصحاء كمجموعة سيطرة . منعت الارانب ضد سائل الكيس العدري, نقيت الاجسام المضادة للاكياس العدرية IgG الناتجة من التمنيع من خلال كروماتوغرافيا التبادل الايوني, بينت النتائج زيادة تركيز المستضدات الدائرة في المصل قبل الجراحة 38.7 مايكروغرام / مل مقارنة بتركيزها بعد ازالة الكيس 25.7 مايكروغرام / مل, ظهرت فروق معنوية تحت مستوى احتمالية اقل من 0.01 , بينت الدراسة الحالية ظهور المستضدات الدائرة في مصل مرضى الاكياس العدرية من خلال الطريقة الغير مباشرة للاليزا (عدة جديدة مصنعة), كشف عنه في مصل المرضى قبل الجراحة في 50 من اصل 52 (96.15 %), كشف الاختبار المستضد في 48 من اصل 52 (92.30 %) لمصل المرضى بعد الجراحة.

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