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T-Cells Proliferation and Serum Cytokine levels in Type 1 Diabetic Children

Author: Eman Mahdi Saleh ايمان مهدي صالح
Journal: Al-Kindy College Medical Journal مجلة كلية الطب الكندي ISSN: 18109543 Year: 2011 Volume: 7 Issue: 2 Pages: 16-26
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: There is plenty of evidence suggesting that involvement of several groups of viruses in the development and / or acceleration of Type 1 Diabetes Mellitus (T1DM). Objective: To analyze the T- cell proliferation in the presence of Coxsackie virus B5 (CVB5), Polio and Adenovirus antigens in addition to assessment of Interferon- gamma (IFN-γ), Interleukins (IL-10 and IL-6). Methods: In 60 Iraqi T1DM children with recent onset of T1DM, Lymphocyte proliferation was analyzed using Methylthiazol tetrazolium (MTT) assay by culturing Peripheral Blood Lymphocytes (PBLs) with Coxsackie Virus B5 (CVB5), Adenovirus, and Polio vaccine. Serum Interferon-γ, IL-10 and IL-6 were quantified by sandwich ELISA. Results: No significant differences were shown in the PBL proliferative percentage in response to Con-A mitogen and tested viruses (CVB5 and Adenovirus) between T1DM and healthy controls, but it showed a significant decline in patients in response to Polio vaccine. Higher significant serum levels of IFN-γ, IL-10, and Il-6 were observed in the investigated patients compared to controls (p<0.05). Mean PBL proliferative percentage in response to tested viral antigens was correlated with the serum IFN-γ, IL-6 and IL-10 levels. Conclusions: In children with new- onset diabetes, mean proliferative percentage of Peripheral Blood Lymphocytes was generally decreased. A significant elevation of serum levels of IFN-γ, IL-10 and IL-6 were observed, which is significantly correlated to mean proliferative responses of PBL to viral antigens.


Article
Cytokines Profile in Newly Diagnosed Children with Type 1 Diabetes Mellitus

Author: Eman M. Saleh * BSc, PhD د. ايمان مهدي صالح
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2009 Volume: 51 Issue: 3 Pages: 295-299
Publisher: Baghdad University جامعة بغداد

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Abstract

Summary :Background: Cytokines have long been implicated in the pathogenesis of autoimmune diabetes in a number of studies, and playing a role in the initiation of β-cell damaging process. The objective of this study is to gain more understanding about the role of cytokines in initiation of T1DM, through assessment of IFN-γ, IL-10 and IL-6 in diabetic patients.Patients and methods: A total of 60 patients who were newly diagnosed as having T1DM (diagnosed less than five months) were included in the present study. Fifty apparently healthy control subjects were underwent the measurement of serum IFN-γ, IL-10 and IL-6 by ELISA. Results: Higher serum levels of IFN-γ, IL-10, and Il-6 were observed in the investigated patients ≤10 years (75.60, 104.92, 147.6 pg/ml respectively) compared to controls (42.66, 57.01, 80.4 pg/ml respectively). The statistical analysis revealed a significant difference between patients and controls (P1= 0.005, 0.003, 0.036 respectively). The mean levels of serum IFN-γ, IL-10, and Il-6 were also significantly elevated in >10 years old patients (70.78, 84.22, 171.8 pg/ml respectively) than controls (40.39, 59.50, 81.6 pg/ml respectively), (P1= 0.006, 0.037, 0.04 respectively). A statistically difference of mean IL-10 concentration appears between patients in both age groups (P2= 0.04). No significant differences appear in the mean serum concentrations of IFN-γ, and IL-6 between the two age groups (P2=0.73, 0.07 respectively).Conclusions: In children with diabetes, a significant elevation of serum levels of IFN-γ, IL-10 and IL-6 were observed.Key Words: T1DM, IFN-γ, IL-10 and IL-6

Keywords

: T1DM --- IFN-γ --- IL-10 and IL-6

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