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Article
Effect of Honey on Mice in Vitro Fertilization (IVF)
تأثير العسل على الاخصاب الخارجي في الفئران

Author: Wasnaa Hatif Mohammed
Journal: Engineering and Technology Journal مجلة الهندسة والتكنولوجيا ISSN: 16816900 24120758 Year: 2014 Volume: 32 Issue: 1 Part (B) Scientific Pages: 107-113
Publisher: University of Technology الجامعة التكنولوجية

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Abstract

Traditionally, honey is frequently consumed by the local population as a nutrient, as well as for the enhancement of fertility and vitality. This study concerning the effects of honey on sperms motility, abnormality, dead sperm, and in vitro fertilization, after 35 days of honey administration orally. 18 adult male mice were divided into one control and two treated groups containing 6 mice in each group. Treated groups received honey in two different doses i.e 1.2 and 1.8 g/kg body weight, the results showed significant (P<0.05) increase in oocyte maturation, fertilization by in vitro fertilization (IVF) and cleavage of oocyte by IVF between the three groups. Also there is significant increase in sperms motility. Decreased percentage of dead sperms between control and the treated groups and significant decrease in abnormalities of sperms in mice between the three groups.

تقليدياً يعتبر العسل من المواد الغذائية الاستهلاكية للسكان المحليين وكذلك يعتبر من محسنات الخصوبة والفعالية. يتناول البحث دراسة تأثير العسل على حركة الحيوانات المنوية, والحيوانات المنوية المشوهه والميتة وعلى الاخصاب الخارجي بعد تجريع العسل عن طريق الفم لمدة 35 يوم. تمت الدراسة على 18 ذكر فئران بالغ وقسمت الى ثلاث مجاميع المجموعه الاولى هي مجموعة السيطرة ومجموعتين تم تجريعهم العسل وتحتوي كل مجموعة على ستة ذكور فئران. المجموعتين التي تم تجريعها بالعسل بتركيزين مختلفين الاول 1.2 والثانية 1.8 غم /كغم من وزن الجسم, واظهرت النتائج ان هناك فروقات معنوية لزياده نسية نضج البويضات والاخصاب عن طريق الاخصاب الخارجي وكذلك انقسام الاجنة التي لقحت بالاخصاب الخارجي بين المجاميع الثلاثة. وكذلك هناك زيادة معنوية في حركة الحيوانات المنوية. والتقليل من نسية الحيوانات المنوية الميتة بين مجموعة السيطرة والمجاميع المعاملة. وهناك نقصان مميز في نسبة تشوهات الحيوانات المنوية للفئران بين المجاميع الثلاثة.

Keywords

Honey --- IVF --- Sperm


Article
The Role of Asymptomatic Bacterial Vaginosis in Women Undergoing Intracytoplasmic Sperm Injection in Predicting Successful Pregnancy

Authors: Muayad S. Abood --- Lubna A. Al-Anbari --- Hourah Sh. Atiea
Journal: Iraqi Journal of Embryos and Infertility Researches المجلة العراقية لبحوث الأجنة والعقم ISSN: eISSN: 26166984 / pISSN: 22180265 Year: 2018 Volume: 8 Issue: 2 Pages: 21-26
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background:Bacterial vaginosis (BV)is a polymicrobial clinical syndrome resultingfrom replacement of the normal hydrogen peroxide producing Lactobacillussp. BV is the most common lower genital tract disorder among women ofreproductive age (pregnant and non-pregnant) and the most prevalent cause ofvaginal discharge and malodor.Objectives:To study the role of asymptomatic bacterial vaginosis on outcome inpatients undergoing ICSI.Patients and Methods:This prospective study was undertaken in the High Instituteof Infertility Diagnosis and Assisted Reproductive Techniques / Al-NahrainUniversity. During the period from September 2017 – April 2018. A total of 100infertile women .All of them were underwent controlled ovarian hyper stimulationfor intra cytoplasmic sperm injection cycle. Antagonist oocyte maturationused as ovulation induction protocol in all the cases. Assessment of oocyte maturationand embryo quality is done to the all the cases, bacterial vaginosis smearwas collected on the day of oocyte retrieval. B.V. smear diagnosis by Amsel’sCriteria and Nugent’s Scoring.Results: TThere was no significant difference in mean age of pregnant andnon-pregnant ladies, 29.40 ±6.47 years versus 30.78±6.84 years, respectively(P= 0.333).In addition the frequency of women under 35 years age was comparablein the two groups with no statistical significant difference, 34 (79.1%) versus33 (73.3%), respectively (P = 0.528). Wide variation and non-normal distributionwas the role followed by the duration of infertility concerning sub-fertilewomen participating in this study. However, no significant difference was observedin the duration of infertility between pregnant and non-pregnant ladies,6.00 (5.00) years versus 6.00 (5.00) years (P = 0.289). Comparison of basal hormonallevels between pregnant and non-pregnant groups revealed no significantdifference despite the presence of some minor differences in mean hormonallevels between the two groups (P> 0.05). Comparison of oocytes characteristicsbetween pregnant and non-pregnant ladies was conducted and revealed no significantdifference in mean total number retrieved oocyte (TNO), ruptured oocytes(RO), abnormal oocyte (AO), germinal vesicle oocyte (GV), metaphase I oocyteand metaphase II oocytes (P > 0.05), table Mean total number retrieved oocyte(TNO), ruptured oocytes (RO), abnormal oocyte (AO), germinal vesicle oocyte(GV), metaphase I oocyte and metaphase II oocytes was 10.81 ±4.95 versus 10.42±6.30, 0.44 ±0.73 versus 0.36 ±0.65, 1.09 ±1.56 versus 0.84 ±2.00, 0.91 ±1.00 versus0.89 ±1.47, 1.51 ±1.49 versus 2.04 ±2.09 and 6.84 ±4.01 versus 6.22 ±3.91 in pregnant andnon-pregnant groups, respectively. Mean fertilization rate was higher in pregnant ladies thanthat of non-pregnant ladies, 62.19 ±22.23 versus 57.59 ±24.95, respectively; however, the differencewas no statistically significant (P = 0.365).Mean grade I embryo number was also significantlyhigher in pregnant women than that of non-pregnant women, 2.53 ±1.53 versus 1.89±1.27, respectively (P = 0.034), Mean grade II embryo number was 0.86 ±1.52 versus 0.89±1.28 in pregnant and non-pregnant ladies, respectively and the difference was not significant(P = 0.925), moreover the difference in mean grade III embryo number between pregnant andnon-pregnant women was insignificant (P = 0.321), 0.30 ±1.01 versus 0.31 ±0.50, respectively.Rate of asymptomatic bacterial vaginosis as determined by gram stain, whiff test, wet mountand Amsel’s criteria was 32.6 % in pregnant women compared to 73.3% in non-pregnantgroup, being highly significant higher in those women who failed to get pregnant (P<0.001).Conclusion: Rate of asymptomatic bacterial vaginosis was 32.6 % in pregnant women comparedto 73.3% in non- pregnant group, being highly significant higher in those women whofailed to get pregnant.


Article
Evaluation of Serum TNF-α in Insulin Resistant Non PCOS Pa - tients Undergoing ICSI: A Randomized Clinical Trial

Authors: Manal T. Al-Obaidi --- Mohammad O. Selman --- Manar Alhoda Z. Al-Faqheri
Journal: Iraqi Journal of Embryos and Infertility Researches المجلة العراقية لبحوث الأجنة والعقم ISSN: eISSN: 26166984 / pISSN: 22180265 Year: 2018 Volume: 8 Issue: 2 Pages: 27-35
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background:With the rapid evolution of assisted reproductive technologies,more and more studies are concerned about the possible influential factors onthe success rates of these procedures, with the efforts are now mainly directedtoward defining these factors in a trial to increase these rates. Of these factors,inflammation is considered among the most remarkable and is in the focus ofthe updated studies. However, most of these studies are evaluating the role ofinflammation in patients with polycystic ovarian syndrome(PCOS) , while theimpact of this inflammation in insulin resistant non PCOS patients is underestimatedand not well evaluated .Objectives:This study is a trial to elucidate the importance of one of the inflammatorymediators , Tumor Necrosis Factor-alpha (TNF – α) , in insulin resistantpatients undergoing IVF ( ICSI ) cycles .Patients and Methods:A group of 27non-PCOS infertile patients were selectedfor this clinical randomized prospective study from patients attending thehigh institute for infertility diagnosis and assisted reproductive technologies /Al-Nahrain University and subjected to intra-cytoplasmic sperm injection (ICSI)procedure. BMI was measured for all patients and they were classified accordingto WHO classification into 3 groups : Acceptable , overweight and obese . Fastingplasma level of glucose and insulin was investigated and insulin resistanceindex (HOMA) was calculated. Serum TNF-α has been measured using ELISAand the relation with insulin resistance was investigated. Finally, the pregnancyrate, as a primary reproductive endpoint was compared according to TNF -a andinsulin resistance levels between different study subjects.Results: Of the 27 infertile patients , 17(63 %) were non obese and 10 (37%)were obese , 15(55.5%) were having HOMA index < 1.6 and 12 (44.5 %) werehaving HOMA index ≥ 1.6 . BMI was found to be positively correlated with higherHOMA index ( P = 0.01 and R= 0.4 in spearman correlation). Investigating therelation between TNF-α with BMI revealed that TNF-α levels are increasing withthe increment in BMI, However , the difference in the mean TNF-α between thethree BMI groups failed to achieve statistical significance (P=0.1). In contrastto that, when stratifying for HOMA index , TNF-α was significantly correlatedwith it ( spearman correlation R= 0.4 , P=0.01 ) , and patients with HOMA index≥1.6 were found to have higher levels of TNF-α (The effect of insulin resistance was also implicated on pregnancy rates , as those with highinsulin resistance were shown to have significantly lower pregnancy rates when compared tothose with low indices of resistance (P = 0.03) . However, the impact TNF-α on pregnancyrates was apparently less significant (P= 0.5).Conclusion: Rate of asymptomatic bacterial vaginosis was 32.6 % in pregnant women comparedto 73.3% in non- pregnant group, being highly significant higher in those women whofailed to get pregnant.


Article
THE EFFECT OF DIFFERENT CULTURE MEDIA ON IN VITRO FERTILIZATION OF MICE

Authors: Mohammed A. Ibrahim --- Hazim I. Al-Ahmad --- Wasnaa H. Mohammed
Journal: Al-Nahrain Journal of Science مجلة النهرين للعلوم ISSN: (print)26635453,(online)26635461 Year: 2010 Volume: 13 Issue: 4 Pages: 147-150
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

The effect of different types of media on in vitro fertilization (IVF) of mice oocyte wasinvestigated. IVF was carried out in RPMI-1640 medium at recommended temperature (37 C) andpH (7.4). The obtained results showed that 63.73% of used oocytes were fertilized, and thepercentage of abnormal embryos was 27.58%. While, using other culture media at sametemperature and pH, gave low percentage of fertilization and higher yield of abnormal embryos.Thus, using Medicult IVF, Earl s and TCM-199 were gave fertilization percentages equivalent to38.75, 42.42 and 61.45% respectively; whereas, the percentages of abnormal embryos were 32, 35.7and 33.89 % respectively.The reported results indicate that composition of various types of culture media have significanteffect on the rate of fertilization and abnormal embryos.


Article
In Vitro Fertilization of Immature oocytes by Testicular Sperm: Animal Model for Azoospermic Infertile Patients

Author: Saeeda A. M. Alanssari
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2007 Volume: 49 Issue: 4 Pages: 394-399
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Men with azoospermia require testicular and epididymal sperm aspiration for intracytoplasmic sperm injection. Women over 37 years of age demonstrate an increase number of immature oocytes after induction of ovulation. The development of a technique for in vitro maturation (IVM) and fertilization (IVF) of the oocytes using testicular, epididymal and vassal sperm (TS, ES, VS, respectively) will be of therapeutic value for the treatment of azoospermic patients. Objectives: The goal of the study was to develop an animal model for the treatment of infertile couples with obstructive azoospermia and immature oocytes. Materials and Methods: Canine ovaries and testes were collected from a local animal clinic. Immature oocytes were retrieved from the ovaries and cultured in modified tissue culture medium (MTCM). The mature oocytes were in vitro fertilized by TS, ES, and VS. The normality of the fertilized oocytes was studied. Results: Sperm motility index was significantly higher (P<0.01) in sperm retrieved from the vas deference compared to ES and TS. The concentration of the sperm was significantly higher (P<0.05) in the testes compared to epididymis and vas deference while VS and ES normal morphology were significantly (p<0.01) better than TS. Viable oocyte percentage was significantly higher (P


Article
The level of 17-beta estradiol in follicular fluid for patients undergoes IVF as correlation with the pregnancy rate
مستوى هورمون 71 بيتا استراديول في السائل الجريبي للمريضات اللواتي خضعن لعمليات اطفال الانابيب و علاقته مع معدل الحمل

Authors: Salam S. Ahmed --- Mutaz S. Ahmeid --- Muhana O. Hussein
Journal: karbala journal of pharmaceutical sciences مجلة كربلاء للعلوم الصيدلانية ISSN: 70272221 Year: 2017 Issue: 12 Pages: 120-130
Publisher: Kerbala University جامعة كربلاء

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Abstract

Estradiol (E2) is a steroid hormone produced mainly by the ovary and placenta and in small amounts by adrenals and testes. Estradiol is in equilibrium with estrone, which can be converted to estriol by the liver and placenta. Like for LH-FSH-progesterone, measurement of estradiol concentration is an essential biochemical tool for the investigation of fertility. So that this study was done to assess the follicular fluid E2 in early follicular phase as a predictor of pregnancy rate among females undergoing in vitro fertilization (IVF) in Iraqi patient. The study population consisted of women undergoing in vitro fertilization and aged between 18 - 45 years. The medical records of 56 infertile patients undergoing IVF in the program at center of Baghdad center in the period between February 2015 and July 2015.This study resulted in a pregnant women were 11 (20%) while the non-pregnant women were 45 (80%). The mean levels of E2 fluctuated among different age groups showing significantly with age >35 as compared with another group. The mean levels of E2 in follicular fluid fluctuated among different BMI groups showing that mid 30 year women had more chance to have pregnancy through IVF, and there's no significant between pregnant and non-pregnant. When related to BMI, IVF result showed that the chance of IVF success increased with decreased BMI. This implies that slim women had more chance to have pregnancy through IVF. Furthermore, level of E2 in follicular fluid couldn't be used in IVF as a predictor success of pregnancy.

هرمون استراديول ينتج أساسا من المبيض والمشيمة و كميات صغيرة منه من الغدة الكظرية والخصيتين. وهو في حالة منالتوازن مع إيسترن، التي يمكن تحويلها إلى استريول بالكبد والمشيمة. وهو مثل هورمونات LH-FSH- البروجسترون من حيثكون تركيز الاستراديول ضروري لتحقيق الخصوبة. في هذه الدراسة تم تقييم E2 في السائل الجريبي في مرحلة ما قبل التبويضكمنبئ لمعدل الحمل بين الإناث اللواتي تمر بالاخصاب في الأنابيب )اطفال الانابيب(لالمرضى العراقيين . هذه الدراسة اجريت- على النساء اللواتي تمر بالاخصاب في الأنابيب، والذين تتراوح أعمارهم بين 54 81 سنة. تم عمل مع عدد من المريضات 45. يعانين من العقم وخضعن لعمليات اطفال الانابيب في مركز بغداد للعقم في الفترة ما بين شباط 5184 وتموز 518411 %(. وان متوسط ( 51 في المائة( بينما كانت النساء غير الحوامل 54 ( وأسفرت هذه الدراسة ان النساء الحوامل يمثلن 88مستويات E2 تغيرت بين مختلف الفئات العمرية في السائل الجريبي إلى حد كبير مع التقدم في السن اكبرمن 54 سنة مقارنةبمجموعة أخرى.ولقد تذبذبت مستويات ال E2 فيم بين مختلف فئات مؤشر كتلة الجسم تبين أن المرأة لها منتصف السنة 51فرصة أكبر للحمل عن طريق التلقيح الاصطناعي وانه لا يوجد فرق ملحوظ بين النساء الحوامل وغير الحوامل. وعندما يقارنبمؤشر كتلة الجسم فقد أظهرت النتائج أن فرص نجاح عمليات اطفال الانابيب زاد مع انخفض مؤشر كتلة الجسم . وهذا يعني أنالمرأة ذات الوزن الاقل لها فرصة أكبر للحمل عن طريق اطفال الانابيب. وعلاوة على ذلك، لا يمكن استخدام مستوى ال E2في السائل الجريبي لعمليات اطفال الانابيب كعامل متنبئ بالحمل

Keywords

Follicular fluid --- E2 --- IVF --- Pregnancy rate


Article
Role of leukemia inhibitory factor(LIF) gene variation on implantation rate following IVF program in PCOS and non PCOS women

Author: Zena F. Hussein1̽ , Bushra J. Al- Musawi 2 , Ismail H. Aziz3 , Saad S. Al-Dujaily 4
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2018 Volume: 17 Issue: 2 Pages: 57-67
Publisher: Baghdad University جامعة بغداد

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Abstract

Implantation of the blastocysts into the maternal uterus is a crucial step in mammalian reproduction, which is controlled by a number of complex molecules like hormones, cytokines, and growth factors and their cross talk. A network of these molecules plays a crucial role in preparing receptive endometrial and blastocysts. This study aimed to found out the role of Leukemia inhibitory factor (LIF) , gene expression, concentration and genetic polymorphisms in the endometrial that may interfere with implantation process of polycystic ovary syndrome(PCOS) and non-PCOS women. A convenient blood (6ml)sample of 80 infertile women undergoing in vitro fertilization (IVF) program were intentionally divided according to the cause of infertility into 40 healthy women their husbands complaining from male infertility factors, and 40 infertile women with polycystic ovary syndrome. Leukemia inhibitory factor( LIFs) were measured on ovulatory and luteal phase of cycle (CD14-CD16,17) at the day of ovarian pickup and embryo transfer by using quantitive polymerase chain reaction(qPCR ), Elisa technique ,polymerase chain reaction(PCR) and sequencing to determine any genetics polymorphisms in LIFgene study. Results of the present study indicate that no mutation was detected in LIF gene in the healthy and PCOS women. The LIF gene expression in addition to levels of serum leukemia inhibitory factors are more valuable in predicting the pregnancy out come in infertile PCOS women than in non-PCOS women .This result can be utilized to be used as predictors of implantation window for successful implantation and pregnancy.


Article
Effect of Ovarian Stimulation Protocol on Embryo Quality in IVF-ICSI

Authors: Mufeda A. Jwad --- Ban A.Abdulmajeed --- Ula M. Al-Kawaz
Journal: Iraqi Journal of Embryos and Infertility Researches المجلة العراقية لبحوث الأجنة والعقم ISSN: eISSN: 26166984 / pISSN: 22180265 Year: 2018 Volume: 8 Issue: 1 Pages: 55-63
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background:Defects in reproduction that may involve both the male & femalepartners result in the inability to procreate which is known as infertility. Theuse of assisted reproductive technologies (ART’s) has been increasing over thepast three decades. Many efforts have been done to compensate for unsatisfactoryresults in in-vitro fertilization (IVF) procedures, so that patients undergodifferent ovarian stimulation protocols to control retrieval of multiple oocytesin a single cycle. Although ovarian stimulation has an important role in ART’s,it may also have detrimental effects on oocyte quality, embryo quality, and IVFoutcomes.Objectives: To assess the effect of long agonist versus antagonist protocols onoocyte and embryo quality in IVF- Intracytoplasmic sperm injection (ICSI).Patients,Materials and Methods:This comparative study was done on the embryos of seventy one infertile femaleswho were undergoing Intracytoplasmic sperm injection (ICSI) treatmentregardless to the presence or absence of previous trials. These patients weredivided into two groups, 45 of them treated with agonist protocol and other 26patients treated with antagonist protocol. First oocyte retrieved was used forfurther assessment. Of the 71 oocytes, there were eight immature oocytes (3 atgerminal vesicle (GV) stage, 5 at metaphase I (MI) stage) and three abnormaloocytes, and these were excluded from the following ICSI treatment. Assessmentand comparison of oocyte maturity, fertilization rate, cleavage rate and resultingembryo quality were done.Results:The results of the present study showed significant difference (p<0.05)in oocyte number and maturity but no significant (p>0.05) difference betweenthe group treated with long agonist and those on antagonist protocol regardingfertilization rate, cleavage rate, and embryo quality.Conclusions:The type of protocol used in in-vitro fertilization has no detrimentaleffect on oocyte or embryo quality.


Article
Patterns of infertility among couples attending IVF center in maternity teaching hospital in Erbil
أنماط العقم بين الأزواج المراجعين لمركز التلقيح الصناعي في مستشفى الولادة التعليمي في أربيل

Authors: Samir M. Othman --- Avesta S. Ahmed
Journal: Zanco Journal of Medical Sciences مجلة زانكو للعلوم الطبية ISSN: 19955588/19955596 Year: 2016 Volume: 20 Issue: 3 Pages: 1467-1475
Publisher: Hawler Medical Univeristy جامعة هولير الطبية

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Abstract

Background and objective: An understanding of certain socio demographic, clinical characters and causes of infertility among couples is important for improving management of this problem. This study aimed to assess the clinico-epidemiological pattern of infertile couples and to find out the association of infertility with various socio-demographic characteristics of infertile couples.Methods: This is a case record review study carried out from March 2013 through April 2014. It comprised a retrospective review of a convenience sample of 1158 medical records of infertile couples attending the infertility center in Maternity hospital in Erbil city. Data collected; based on secondary data that was obtained from infertile couples medical records from the center. The medical records included socio-demographic characteristics of couples, data about the gynecological, surgical and medical history of wives and medical and surgical history of husbands. In-depth analyses of the data obtained from files were done by using SPSS version 19.Results: Of the 1158 infertile couples, 727 (63%) had primary, and 431 (37%) had secondary infertility. The age range of wives was 15-48 years with mean ± SD of 31.25 ± 7.0 years and age range of husbands was 17-71 years with mean ± SD of 35.28 ± 8.0 years. The duration of infertility ranged between 1-27 years with mean ± SD of 6.2 ± 4.8 years. The proportion of primary infertility was significantly higher (91.5%) among women less than 20 years of age, while secondary infertility was higher (48%) among those aged 40 years and more. Female causes accounted for 32%, male causes account for 26%, combined cause 18% and 24% of them had unknown causes for infertility. Abnormal seminal fluid analysis parameter was significantly observed among primary infertile husband. Among wives, the infertility factors were the menstrual disorder, miscarriage and hormonal disorder.Conclusion: Primary infertility was more prevalent among the study sample. Infertility was significantly associated with age, occupation and history of previous marriage among wives


Article
Effect of Glycyrrhiza glabra extract on IVF outcome in Mice : Experimental model for mammals
تقييم تاثير مستخلص عرق السوس على معدل الاخصاب والنمو الجنيني في الزجاج باستخدام الفئران كموديل للبائن

Authors: Alaa S. Al-Saadi الاء السعدي --- Saad, S. Al-Dujaily سعد الدجيلي
Journal: Jornal of Biotechnology Research Center مجلة مركز بحوث التقنيات الاحيائية ISSN: 18151140 Year: 2009 Volume: 3 Issue: 2 Pages: 80-89
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Objective: To evaluate and examine the effect of Glycyrrhiza glabra extract (GgE) on the fertilization rate and embryonic development in vitro using the mice as a model for mammals. Design: Prospective study. Methods: Seven hundred forty ova were collected from superovulated ( SUO) females mice .Glycyrrhiza glabra extract was prepared and used for in vitro activation of caudal epididymal sperm . In vitro fertilization was performed using both 10% GgE mixed with IVF medium for 367 ova (treated group) and IVF medium alone for 373 ova(Control group) . Fertilization rate and normal development of different early embryonic cleavages stages was recorded. Results : There was a significant ( P<0 .05) increase in the fertilization rate (FR ) of SUO mice oocytes (53.89%)by using 10% GgE Compared to IVF medium alone in SUO group(36.82%) . Addition of 10% GgE to the IVF medium, the number of 2-cell and 4-cell embryonic stages of SUO mice was significantly (P<0.05) higher than that of control mice embryos cultured with IVF medium alone (60% and 60% Vs. 51% and 54%, respectively). Conclusion: The investigation showed that the Glycyrrhiza glabra extract may contain many growth factors, and energy sources that supporting the FR and normal development of early cleavage stages of mice embryos in vitro .This result can be utilized for IVF program in mammals.

استخدمت سبعمائة واربعين بويضة جمعت من فئران محفزة مبايضها هرمونيا . حضر واستخدم عرق السوس في تنشيط النطف البربخبة في الزجاج . انجزت عملية الاخصاب في الزجاج باستخدام 10% من مستخلص عرق السوس لمجموع 367 بويضة . كما اجريت عملية الاخصاب في الزجاج باستخدام وسط زرعي خاص للاخصاب في الزجاج لا يحوي عرق السوس لمجموع 373 بويضة . سجلت معدلات الاخصاب والنمو الجنيني لمختلف مراحل الانقسام المبكر . النتائج: سجلت الدراسة زيادة معنوية ( P<0.05) في معدل الاخصاب للبويضات المحفزة وباستخدام 10% من مستخلص عرق السوس مع الوسط الزرعي الخاص بالاخصاب في الزجاج ( 53.89% ) بالمقارنة بالوسط الزرعي الخاص بالاخصاب في الزجاج وحده ) 36,82) % . عند اضافة 10% عرق سوس الى الوسط الخاص للاخصاب في الزجاج, لوحط بان عدد الخلايا الجنينية في مرحلة ( 4 و8 ) خلية اعلى من استخدام الوسط الزرعي وحده ( 60 و 60) %مقابل (51 و 54) % على التوالي . الاستنتاجات:اظهرت نتائج الدراسة الحالية بان مستخلص عرق السوس ربما يحتوي على عدد من عوامل النمو ومصادر طاقة التي ساندت معدلات الاخصاب والنمو الطبيعي للاجنة في مختلف مراحل الانقسام المبكر في الفئران في الزجاج . هذه النتيجة يمكن الاستفادة منها في برامج الاخصاب في الزجاج في اللبائن .

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