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STUDY OF AMERICAN COCKROACH ALLERGY DISTRIBUTION AND ITS ASSOCIATION WITH SOME HLA- DQ AND - DR ALLELES IN ATOPIC PATIENTS IN BASRAH PROVINCE / IRAQ
دراسة انتشار ارجية الصرصرالامريكي وارتباطها مع بعض الألائلHLA- DQ و – DRلمرضى الحساسية في محافظة البصرة / العراق

Author: Huda K. Kareem * Fawziah A. Abdullah ** Dhia K. Kareem
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: 18138497 Year: 2016 Volume: 15 Issue: 1 Pages: 209-227
Publisher: Basrah University جامعة البصرة

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Abstract

Ninety six for each healthy(n=96)and atopic,(n=96)individuals duals from the same geographical region, paired by sex and age, their sera specific IgE antibodies were estimated by enzyme linked immune sorbent assay test (ELISA) and genotyped by polymerase chain reaction based onHLA-DQB1*0602, HLA-DQB1*0604 andHLA-DRB1*12.The specific IgE based on ELISA results revealed that Out of 96 only59 (61.5%) of atopic patients were sensitive to CR allergen. The association between sensitivity to CR allergen and age was considered to be not statistically significant (P>0.05).However the higher rate of CR allergens sensitivity(62.9%) was observed in first age group(<45 year) of atopic patients. In contrast the effect of sex on sensitivity to CR allergens was considered to be statistically significant (p<0.05) and the higher rate of sensitivity(75.6%) was observed in atopic patients males.The overall differences in the HLA-DQB1*0602, HLA-DQB1*0604 and HLA- DRB1*12 alleles frequency between patients and controlswerestatistically (p<0.05).According to the results of risk factors statistical analysis values(p:value = 0.0001; OR:84.3481; CI 95%: 5.065- 1404.520) HLA-DQB1*0602 allele was associated with susceptibility of patients to the CR allergy.The overall differences in the HLA-DQB1*0602, HLA-DQB1*0604 and, HLA- DRB1*12 alleles distribution between atopic patients and controls were statistically highly significant (p<0.01) concerning the effect of the age and sex. In general the allele HLA- DRB1*12 was not observed in both atopic patients and controls in contrast HLA-DQB1*0602 was present in atopic patients only while HLA-DQB1*0604 appeared in both patients and controls with different frequencies The older atopic patients showed higher frequency (61.8% ) for the HLA- DQB1*0602 allele . In contrast higher frequency of HLA- DQB1*0604 allele occurred in younger patients(40.3%) . According to sex ,the higher frequency of HLA-QB1*0602allele was observed in males patients(31.7%)while the allele HLA-DQB1*0604 higher frequency(17.9 %)was observed in the females of the control group. The overall frequency of HLA-DQB1*0602(60.4%)orHLA- DQB1*0604(39.6%) as a single allele was observed in the seropositive or seronegative atopic patients. The seropositive showed higher frequency(35.6 and 15.3%) for HLA- DQB1*0602 and HLA- DQB1*0604 respectively

Keywords

ELISA --- IgE antibodies --- Allergy.


Article
THE RELATIONSHIP BETWEEN HLA-DRB1*0103 AND HLA-DQA1*0201 GENOTYPES AND SPECIFIC IGE RESPONSES TO UNRELATED ALLERGENS IN ATOPIC PATIENTS
العلاقة بين النمطين الجينيينHLA-DRB1*0103 and HLA- DQA1*0201واستجابةIgE الخاصة بالمستارجاتعديمة الصلة في مرضى الحساسية

Author: Falih H.Mezban*Fawziah A. Abdulla فالح حمود مزبان* فوزيه علي عبدالله**
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: 18138497 Year: 2014 Volume: 13 Issue: 1 Pages: 260-273
Publisher: Basrah University جامعة البصرة

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Abstract

Because of the pivotal role of the human leukocyte antigen (HLA) class II molecules in regulating the immune response and their extensive polymorphism, it is not surprising that particular HLA class II alleles have been implicated in susceptibility to allergic diseases and in restriction of the IgE responses to a variety of allergens. We investigated the relationship between HLA-DRB1*0103 and HLA- DQA1*0201 genotypes and allergies to amoxicillin(AX) and love bird (LB) and explored their restriction of IgE responses to these allergens.Enzyme Linked Immunosorbent Assay test (ELISA) was used to examine the specific IgE antibodies in the sera of 175 subjects including 145 atopic patients and 30 healthy individuals without any allergic reaction. All of them were chosen for HLA genotyping by polymerase chain reaction . Compared with seropositive all control and seronegative individuals showed negative PCR HLA genotyping results.The higher overall rate (39.4%) of PCR positivity was observed inseropositive patients with positive PCR HLA-DQA1*0201 genotyping results .Also PCR HLA-DQA1*0201 genotyping revealed the higher rates of the PCR positivity in the males and females of the second age group in comparison with those of the first age groups but without significant difference (P > 0.05). The same result was also observed in positive PCR result for the patients with HLA-DRB1*0103 and those with both HLA -DRB1*0103 and HLA-DQA1*0201 as the non-significant higher PCR positive rates was observed in the males and females of the 2ndage groups.Also the females of the second age group showed the higher PCR positivity rates (100%,50% and30%)for HLA-DQA1*0201 and HLA-DRB1*0103) and both respectivelyELISA results revealed that the overall rate of seropositivity in tested individuals to amoxicillin(AX) or love bird dropping extract (LBDE) allergens was 54.9% .The females were not significantly different (P ≥ 0.05) from males and they showed the higher rate of seropositivity against AX(57.9%)and LBDE(58.9%)allergens.In contrast there was no significant effect(P > 0.05)for the age on the seropositivity against LBDE and the patients of the 1st age group showed higher rate (55.9%) of seropositivity in comparison with rate (43.7%) of 2ndage group patients

لبعض الاليل الجينات ا لخاصة بمستضدات كريات الدم البيضاء من الصنف الثاني في الانسان علاقه بامراض الحساسية وتحديد استجابة الضد IgE لانواع مختلفه من المستارجات. وذلك بسبب دورها الحيوى في تنظبم الاستجابه المناعية والتغير المفرط في اشكالها وقد تم التحقق من العلاقة بين النمطين الجينيينHLA-DRB1*0103 and HLA- DQA1*0201والحساسية للاموكسيسيلين وروث طيور الحب و التحري عن دورها في تحديد استجابة IgE لهذه المستارجات .استخدم اختبار ELISA للكشف عن الضد IgE في مصول 175 شخص تتضمن145 مريض بالحساسية و 30شخص سليم جميع هؤلاء الاشخاص تم اختيارهم للتنميط الجيني بواسطة تفاعل سلسلة البلمره PCR.بالمقارنة مع الاشخاص اللذين اظهرو نتائج ايجابية مصلية فان جميع اشخاص السيطره وذوي النتائج المصلية السلبية اظهرو نتائج سلبية في التنميط الجيني بواسطة تفاعل سلسلةالبلمره ولوحظ النمط الجينيHLA-DQA1*0201 باعلى نسبة ايجابية (39.4%)للتنميط الجيني بختبارPCR في المرضى ذوي النتائج المصلية الايجابية كذلك ظهر هذا النمط الجيني باعلى نسبه ايجابية لاختبارPCR في ذكور واناث مرضى الفئه العمرية الثانية بالمقارنة مع مرضى الفئةالعمرية الاولى ولكن بدون فارق احصائي معنوى(P > 0.05).لوحظت نفس النتائج الايجابية لاختبار PCRلمرضىى الفئة العمرية الثانية ذكورا واناثا فيما يخص النمط الجينيHLA-DRB1*0103 كنمط جيني منفردا او مشتركا معHLA-DQA1*0201وبدون فرق احصائي معنوى 0.05) (P > وكذلك اظهرت الاناث من الفئة العمرية الثانية نسب PCRايجابية عالية (100%و50%و30%) وعلى التوالي لكل من النمطين الجينيين DQA1*0201 وHLA-DRB1*0103 وللاثنين معا.اظهرت نتائج ELISA بان اعلى نسبة كلية للايجابية المصلية للاشخاص المفحوصين بمستضدات الاموكسيسيلين وبراز طيور الحب كانت بمقدار54.9%.لم تختلف الاناث عن الذكور احصائيا(P ≥ 0.05) وفي الاشخاص المفحوصين كانت نسبة الايجابية المصلية ضد الاموكسيسيلين(57.9%)ومستارجاات براز طيور الحب(58.9%). لا يوجد تاثير محسوس احصائيا(P > 0.05)للعمرعلى الايجابية المصلية ضد مستارجات براز طير الحيوان لمرضى الفئه العمرية الاولى اظهرو اعلى نسب (55.9%)للايجابية المصلية بالمقارنة مع نسب مرضى الفئه العمرية الثانية (43.7%).

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