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Article
Oral candidal colonization in immunocompromised patients

Authors: Zahraa S. Kasim --- Manahil M. Yehia
Journal: Iraqi Journal of Pharmacy المجلة العراقية للصيدلة ISSN: 16802594 / EISSN 22642522 Year: 2013 Volume: 13 Issue: 2 Pages: 17-26
Publisher: Mosul University جامعة الموصل

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Abstract

Objective: 1. To identify Candida species isolated from saliva of predicatively immunocompromised (cancer, diabetic) patients and control group. 2. To test the susceptibility of all the isolated yeasts against 6 antifungal agents, namely amphotericin B, nystatin, fluconazole, ketoconazole, voriconazole and itraconazole. 3. Then to compare the growth and density of yeasts from immunocompromised patients to those from healthy control group. Patients and Methods: This study included 120 saliva samples from immunocompromised patients, 60 of them with cancer and under treatment, the other 60 patients have uncontrolled diabetes mellitus, attending the Al-Wafa'a Center for diabetic patients and the consultative clinic of Oncology and Nuclear Medicine Hospital. The clinical specimens were collected during the period from January-June 2011. In addition to 60 saliva samples from a healthy individuals. The identification of the isolated yeasts was carried by fluorescent and/or light microscope, culture on different types of media, biochemical tests and API-20 C system. Susceptibility test to six antifungal agents was prepared for each isolate. Results: Eighty seven (72.5%) patients showed the presence of yeasts in their saliva in comparison to 18(30%) from the control group. Eighty four isolates from genus Candida identified into 8 species mainly C. albicans. The isolates showed the best susceptibility to amphotericin B.Conclusions: A higher frequency of yeasts were detected in the immunocompromised patients in comparison to the control group. This is considered a risk factor for infection. Candida albicans is the main isolate but there was a shift to non –albicans Candida spp. in immunocompromised patients. This means that are more resistant to antifungal agents.

الأهداف :.١ تمييز أنواع الفطريات المأخوذة من اللعاب لمرضى ذوي المناعة المنقوصة ظاهريا (الأورام، السكري) و من أفراد أصحاء يمثلون مجموعة السيطرة .٢ أجراء فحوصات الحساسية لكل الخمائر المعزولة باستعمال ٦ أنواع من المضادات الفطرية وهي (amphotericin B, nystatin, fluconazole, ketoconazole, voriconazole and itraconazole ) .٣ مقارنة نمو و كثافة الخمائر المعزولة من المرضى ذوي المناعة المنقوصة مع مجموعة السيطرة. المرضى و طرائق العمل: تضمنت الدراسة ١٢٠ عينة لعاب من مرضى ذوي المناعة المنقوصة، ٦٠ منهم كانوا من مرضى الأورام و تحت العلاج و المرضى ٦٠ الآخرون كانوا يعانون من داء السكر غير المسيطر عليه , وجميع المرضى كانوا من المراجعين للعيادات الاستشارية لمستشفيات ابن سينا و الطب النووي لعلاج الأورام و مركز الوفاء لعلاج مرضى السكري. تم جمع العينات السريرية للفترة من كانون الثاني – حزيران ٢٠١١ . كما تضمنت الدراسة مجموعة السيطرة التي شملت ٦٠ عينة لعاب من أفراد أصحاء . تم تميز الخمائر المعزولة بواسطة الفحص المباشر باستخدام مجهر التألق و/ أو المجهر الضوئي والزرع على أوساط مختلفة و بإجراء الفحوصات الكيميحيوية واستخدام نظام API-20 C . فحوصات الحساسية لكل الخمائر المعزولة فيتم إجراؤها باستعمال ٦ أنواع من المضادات الفطرية. النتائج: ظهرت الخمائر في لعاب(%٥. ٧٢) ٨٧ مريض بالمقارنة مع مجموعة السيطرة.(%٣٠) ١٨ تم تمييز ٨٤ عزلة من جنس المبيضات و صنفت الى ثمانية أنواع وخصوصا المبيضات البيضاء. العزلات أظهرت استجابة جيدة للمضاد الفطري amphotericin B .الاستنتاجات: النسبة الأعلى من الخمائر ميزت في المرضى ذوي المناعة المنقوصة بالمقارنة مع مجموعة السيطرة و يعتبر هذا عامل خطر لإصابة المرضى ذوي المناعة المنقوصة بها. وكانت المبيضات البيضاء أهم الأنواع المعزولة مع ملاحظة التحول إلى المبيضات غير البيضاء في المرضى ذوي المناعة المنقوصة و الذين كانوا اشد مقاومة للمضادات الفطرية.


Article
Determination of Optimal Temperature and pH for Radial Growth of Some Dermatophyte Species Isolated from Leukemia Patients
تحديد درجة الحرارة والاس الهيدروجيني المثلى للنمو الفطري لبعض عزلات الفطريات الجلديه المعزوله من مرضى اللوكيميا

Authors: Muna H. AL-Jubori منى خماس الجبوري --- Alaa M. Hasan الاء محمد حسن
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2015 Volume: 56 Issue: 1A Pages: 95-99
Publisher: Baghdad University جامعة بغداد

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Abstract

The study is concern on determine the effect of different temperatures (25, 28, 30 and 370C), and different pH values (4.5, 5.5, 6 and 8) on the radial growth (mm) of 15 dermatophyte isolates (Microsporum canis 7, Trichophyton rubrum 5, Trichophyton mentagropyhtes 3). The specimens for the current study were collected from nail infections in patients with different type of leukemia whom admitted at Baghdad Educational Hospital, 7th floor. The result revels that the optimum temperature for radial growth was 300C then 280C for all isolates, while the optimum pH for all isolates was 6.

هدف الدراسة هو تحديد تاثير درجات حراريه مختلفه تتراوح من (25, 28 , 30, 37م0), وقيم مختلفه للاس الهيدروجيني(4.5, 5.5, 6 و8) على التوالي على النمو القطري ل 15عزلة من الفطريات الجلدية, Microsporum canis 7) Trichophyton rubrum 5 و Trichophyton mentagropyhtes 3). جميع العينات لهذا البحث جمعت من اظافر مصابة من مرضى اللوكيميا بانواعه المختلفه الراقدين في الطابق السابع/ امراض الدم/ مستشفى بغداد التعليمي. اوضحت النتائج ان درجة الحراره المثلى للنمو القطري للفطريات الجلديه كانت 30م0 ثم 28م0 لجميع العزلات, بينما الاس الهيدروجيني الامثل لجميع العزلات كان 6.


Article
A sample of patients affected with chronic respiratory tract infection
عينة من المرضى المصابين بعدوى الجهاز التنفسي المزمنة

Authors: Abdulaziz Y. Mansoor --- Rassool A. Dabbagh --- Khonaw kader salih
Journal: Zanco Journal of Medical Sciences مجلة زانكو للعلوم الطبية ISSN: 19955588/19955596 Year: 2012 Volume: 16 Issue: 2 Pages: 125-128
Publisher: Hawler Medical Univeristy جامعة هولير الطبية

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Background and objectives: Cryptococcosis is subacute or chronic respiratory disease caused by yeast like fungus called Cryptococcus neoformans. The disease may metastasize to CNS and other parts of the body. The objective of the study is to find the prevalence of this disease in a sample of patients in Erbil.Method: One hundred and twenty eight patients with chronic respiratory tract infection were included in this study. Early morning sputum sample was collected from each patient and subjected to both direct examination and cultureResults: The study showed 24 positive cases of Cryptococcosis, from the total 128 patients examined; 16 of them (66.6%) gave positive culture for Cryptococcus neoformans while 8 (33.3%) gave positive culture for opportunistic species of Cryptococcus. The latter were immunocompromised individuals.Conclusion: It is concluded that looking for Cryptococcus, is quite significant in patients with chronic respiratory diseases.


Article
Antibacterial efficiency of chlorhexidine digluconate 0.2% against oral β- hemolytic streptococci and oral Staphylococcus aureus in immunocompromised patients

Author: Firas H. Qanbar
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2012 Volume: 24 Issue: special issue 2 Pages: 166-169
Publisher: Baghdad University جامعة بغداد

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Background: The use of antimicrobial agents was advocated for a number of years using different compounds thatdelivered through mouth rinses to control intra- and extra-oral disease in immunocompromised patients. The purposeof this research is to find out and to compare between the antibacterial properties of 0.2% chlorhexidine digluconate(CHX)on oral â- hemolytic streptococci and Staphylococcus aureus isolated from patient with renal failure.Materials and methods: â- hemolytic streptococci and Staphylococcus aureus were isolated stimulated salivasamples collected from patients receiving steroids therapy. These bacteria were purified and diagnosed accordingto morphological characteristic, biochemical and antibiotic sucseptibility tests.Results: Agar diffusion technique demonstrated that chlorexidine digluconate inhibited the growth of both types ofisolates, but the antibacterial effect against Staphyllococcus aureus was less than that against â- hemolyticstreptococci.Conclusion: The use of CHX 0.2% as a mouth wash to remove those pathogens from the oral cavity to inhibit theirinfections in immunocompromised patients is highly indicated


Article
The Impact of Duration of Neutropenia in the Immunocompromised Host

Authors: Noaman Abdulateef Abdulrazzaq --- Saad Shawqi Ebada
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2013 Volume: 12 Issue: 2 Pages: 237-243
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT: INTRODUCTION:Infection is a common cause of morbidity andmortality in immunocompromised patients for *Central Public Health Laboratory-Ministry of Health.**Dep.College of Medicine University of Baghdad . whom diagnosis and management requirescrupulous attention and continuing awareness of the microorganisms causing infection in aparticular environment in different groups ofpatients , most are caused by recognized pathogen, although uncommon,some times THE IRAQI POSTGRADUATE MEDICAL JOURNAL VOL.12, NO 2 ,2013 BACK GROUND: Patients with neutropenia can be at low-or high-risk according to the duration of neutropenia, itsdifferential count, the ANC (absolute neutrophil count) in addition to search for any causativeagents to improve the diagnostic and therapeutic outcome. OBJECTIVE: This study focuses on the clinical and microbiological data in relation to the duration ofneutropenia in a sample of immunocompromised Iraqi patients due to leukemia and aplasticanemia. INDIVIDUAL METHODS:150 patients presented with pyrexia, marrow failure from oncology unit of the 7 floor of MedicalCity Hospital, National Center of Hematology-Iraq , Institute and Hospital of radiotherapy andnuclear medicine studied between 2008-2009 ,classified into three groups, fifty for each, with aplastic anemia, acute lymphoblastic leukemia(ALL), acute myelogenous leukemia(AML),all wereinvestigated for WBC count, differential,ANC,Hb,Pcv,platelet count, RBC,Peripheral blood filmfor blast cells,ESR, specifically in those complaining from pyrexia of unknown origin. directexamination ,culture and sensitivity of the urine,stool,body fluids, sputum ,blood, tonsil and skinswab, Widal, rose Bengal tests, besides temperature recording was done for all patients withknown site of infection. RESULTS :Neutropenia of a plastic anemia 5-70 days with a mean of 25 days is associated with a higher riskof infection particularly by the gram-negative enterobacter ,but with a mortality rate of 26%,whilethose suffered from ALL with a range of 11-40 day and a mean values of 16 days with a lessmortality rate 22%.Unfortunatelly AML although gets a range of 7-35 day and a mean values 18day and short lived neutropenia yet it was significantly have a higher mortality rate 52% presumably due to the combined effect of low neutropil count and chemo radiotherapy .Howeversever neutropenia (ANC < 500) is only found in 10 AA,6 AML,and 4 ALL patients, and moderateneutropenia (500 ≤ ANC < 1000) were explored in 7 AA,4 AML,10 ALL. The microbiological investigation shows that gram negative microorganism especially E.coli ispositive in 19 different samples followed by Klebsiella 12 positive sample, then monilia takesplace12 cases, followed by the remaining microorganisms.Lastly pyrexia of unknown origin were found to be the top problem facing immunocompromisedpatients with 20% AA,34%ALL,30%AML followed by chest infection16%AA,22%ALL,15%AML, and then urinary tract infection17%AA,17%ALL,10%AMLfollowed by other types of infections. CONCLUSION:Neutropenia is prolonged in a plastic anemia compared to acute leukemia with the risk ofinfection is directly related to it.G(-ve) enterobacter particularly E-coli is the commonest pathogenisolated.


Article
Molecular diagnosis of Pneumocystis jirovecii pneumonia by using External PCR in immunocompromised patients .
التشخيص الجزيئي لداء ذات الرئة بالمتكيسات الرئوية لفطر Pneumocystis jirovecii باستخدام PCR الخارجي في مرضى العوز المناعي

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Pneumocystis jirovecii is an opportunistic eukaryotic pathogen causing life threating pneumonia ( PJP) in immunosuppressed patients.Detection of Pneumocystis jirovecii Pneumonia in immune suppressed patients like hematological cancer with or without chemotherapy, patients on corticosteroid therapy, other malignancy like bronchogenic cancer by using External Polymerase Chain Reaction (External PCR) and to compare the results with other control group (patients who are immunocompetent suffer from pneumonia).A total of 220 specimens were obtained from patients admitted to Al- Ramadi Teaching Hospital, Clinic excitability of respiratory disease in Al- Ramadi and Oncology Teaching Hospital in Baghdad during the period from February to July 2013. This number composed of 71(32%) specimens were from female while the male specimens were 149(68%). A total of 184 (84%) were immunocompromised patients, They were considered immunocompromised patients because they have ( hematological cancer with or without chemotherapy, patients on corticosteroid therapy, other malignancies like bronchogenic cancer). The other 36 (16%) patients with pneumonia were included as immunocompetent patients (No hematological cancer, No chemotherapy, No immunosuppressive drug like corticosteroid therapy and No malignancy like bronchogenic cancer). A total of 220 specimens which were collected, (consisting of 115 blood, 65 sputum, 40 BAL Specimens )the total 220 specimens (115 blood, 65 sputum and 40 BAL Specimens ) were examined PCR technique ( External and Nested PCR ) to identify Pneumocystis Jirovecii DNA based on the amplification of specific primers. The result of agarose gel electrophoresis revealed that small DNA diagnostic bands were detected at 346 bp for External PCR in all positive specimens. Out of 220 specimens 26 (11.8%) were detected by the mtLSU rRNA External PCR. Regarding the use of External PCR technique for sputum specimens 8 (12.3%) were positive, While BAL 8 (20%) and Blood 10 (8.7%) . PCR is more accurate and better indicative for the presence of Pneumocystis jirovecii Pneumonia. In addition this technique can use specimens which were not stored properly and became non suitable for microscopy.

درس مرض ذات الرئة بالمتكيسات الرئوية لفطر Pneumocystis jirovecii الذي هو مرض انتهازي يصيب كثير من المرضى ضعيفي المناعة.للكشف عن وجود مرض ذات الرئة بالمتكيسات الرئوية لفطر Pneumocystis jirovecii في مرضى العوز المناعي (سرطان الدم مع أو بدون العلاج الكيميائي ، والمرضى على العلاج كورتيكوستيرويد ، والأورام الخبيثة الأخرى مثل سرطان قصبية المنشأ ) . باستخدام تقنية ال PCRالخارجية ومقارنة النتائج مع نتائج الاصابة بمرض ذات الرئة بالمتكيسات الرئوية في مرضى المناعة الكاملة.جمعت 220 عينه من المرضى الراقدين في مستشفى الرمادي التعليمي والعيادة الاستشارية للإمراض الصدريه في الرمادي و مستشفى الأورام التعليمي في بغداد خلال الفترة من فبراير حتي يوليو 2013. وهذا العدد يتكون من 71 ( 32 ٪ ) من العينات من الاناث بينما كانت عينات الذكور 149 ( 68 ٪ ) . اخذت 184 ( 84 ٪ ) من مرضى العوز المناعي (سرطان الدم مع أو بدون العلاج الكيميائي ، والمرضى على العلاج كورتيكوستيرويد ، والأورام الخبيثة الأخرى مثل سرطان قصبية المنشأ ) . اما مرضى المناعة الكاملة كانت نسبتهم 36 ( 16 ٪ ) من المرضى المصابين بالالتهابات الرئوية ويتميزون بأنه ليس لديهم سرطان الدم ، العلاج الكيميائي ، الادوية المثبطة للمناعة مثل العلاج كورتيكوستيرويد ، اورام مثل سرطان قصبي المنشأ . قسمت العينات الكلية (220 عينة) الى 115 عينة دم ، 65 عينة قشع ، 40 عينة غسيل قصبي حويصلي).فحصت جميع العينات (220 عينة) المتمثلة بالإعداد التالية 115 عينة دم ، 65 عينة قشع ، 40 عينة غسيل قصبي حويصلي بواسطة تقنية PCR (الخارجية و المتداخلة ) لتحديد الحامض النووي للمتكيسات الرئوية الفطرية اعتمادا على تضخيم اجزاء معينه من الحامض النووي لفطر Pneumocystis jirovecii. كشفت نتائج الترحيل الكهربائي عن حزم صغيره من الحامض النووي في 346 زوج قاعدة باستخدام PCR الخارجية في جميع العينات الإيجابية. فيما يتعلق بصبغة. العينات الكلية (220 عينة) 26 ( 11.8 ٪ ) كشف عنها بواسطة جين mtLSU rRNA باستخدام PCR الخارجية. لم تكن هناك فروق معنوية ( قيمة P> 0.05) في التوزيع بين الجنسين. فيما يتعلق باستخدام تقنية PCR الخارجية لعينات القشع 8 ( 12.3 ٪ ) كانت ايجابية ، في حين عينات الغسيل القصبي الحويصلي 8 ( 20 ٪ ) وكانت عينات الدم 10 ( 8.7 ٪ ) عينات إيجابية. خلاصة النتائج المذكورة أعلاه أن PCR هو أكثر دقة من طرق الصبغ المباشر وأفضل مؤشرا لوجود مرضى ذات الرئة بالمتكيسات الرئوية. بالإضافة هذه التقنية يمكن استخدام العينات التي لم يتم تخزينها بشكل صحيح و أصبحت غير مناسبة للفحص المجهري


Article
Evaluation of direct immunofluorescent method in the detection of Pneumocystis jiroveci among patients with lower respiratory tract infections

Author: Manahil M. Yehia; Asmaa Z. Sheetawy; Zainalabideen A. Abdulla
Journal: Iraqi Journal of Pharmacy المجلة العراقية للصيدلة ISSN: 16802594 / EISSN 22642522 Year: 2014 Volume: 14 Issue: 1 Pages: 58-62
Publisher: Mosul University جامعة الموصل

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Abstract

Objective: Evaluate the use of direct immunofluorescent method in the detection of Pneumocystis jiroveci from induced sputum of patients with LRT infections. Patients and methods: Thirty patients with LRT infections were included (24 patients were immunocompromised and 6 immunocompetent). The present study conducted for 6 months from January-June, 2011. Smears were prepared from their induced sputa and stained with direct fluorescent antibody stain, then examined under fluorescent microscope.Results: Sputum of 5(16.7%) patients revealed positive results for the presence of P. jiroveci and all of them were immunocompromised (two acute myloid leukemia, one acute lymphoid leukemia, one non-Hodgkin's lymphoma, and one asthma under long coarse of corticosteroid therapy).Conclusion: Direct fluorescent antibody technique is more sensitive than the conventional methods for the detection of P. jiroveci in LRT infections.Keywords: Pneumocystis pneumoniae, Pneumocystis jiroveci, immunocompromised patients.


Article
CONVENTIONAL METHODS FOR THE DIAGNOSIS OF PNEUMOCYSTIS JIROVECII IN IMMUNOCOMPROMISED IRAQI PATIENTS

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Abstract

Background: Pneumocystis jirovecii is the causative agent of pneumocystis pneumonia, one of the most frequent and severe opportunistic infections in immunocompromised patients. Objectives:To determine the possible implication of pneumocystis jirovecii in immunocompromised Iraqi patients with pulmonary infections, and investigate the efficiency of indirect qualitative immunofluorescence diagnosis in compared with conventional stains for the detection of this agent.Methods:A total of 200 clinical samples from 100 immunocompromised patients (70 bronchoalveolar lavage, 21 sputum samples and 9 pleural fluids). One hundred samples from immunocompetent individuals (50 bronchoalveolar lavage, 30 sputum samples and 20 pleural fluids). Detection of pneumocystosis was done by conventional satins and indirect qualitative immunofluorescence technique.Results:Fourteen samples gave positive results by indirect immunofluorescence monoclonal antibody test. Twelve out of 100 samples were positive by each Gomori methenamine silver, modified toluidine blue O stains and Diff-Quik stain (modified Giemsa).Conclusion: Pneumocystis jirovecii is the fundamental opportunistic infection among immunocompromised patients. The indirect qualitative immunofluorescence method gives a promise for use as a primary method for diagnosis of pneumocystis jirovecii pneumonia or as rapid screen to exclude the presence of pneumocystis jirovecii in sputum and bronchoalveolar lavage samples.Keywords: Pneumocystis jirovecii (carinii), pneumocystis jirovecii pneumonia, immunocompromised, Iraqi patients.


Article
Prevalence of Toxoplasma gondii among immunocompromised patients (heamodialysis and cancer) in the province of Thi-Qar-Iraq

Authors: Bassad A.AL-Aboody --- Noor Kadhim Matar AL-Rekaby
Journal: JOURNAL OF THI-QAR SCIENCE مجلة علوم ذي قار ISSN: 19918690 Year: 2017 Volume: 6 Issue: 3 Pages: 101-106
Publisher: Thi-Qar University جامعة ذي قار

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Abstract

The current study was conducted in Thi-Qar province to investigate of Toxoplasma gondii prevalence among renal dialysis and cancer patients by using anti-Toxoplasmosis IgG and IgM by ELISA also the study determined of some factors that may affected the prevalence of infection, such as age, sex, marital status, place of residence and a number of chemical dosing that exposed to it cancer patient or the number of times the dialysis for renal dialysis patients. The results of the current study showed that the prevalence of toxoplasmosis in Thi-Qar province in both renal dialysis and cancer patients and control group were 28% and 36% and 24% respectively. recorded results of Enzyme Linked Immunosorbent Assay ELISA test results for a positive antibody IgG in both renal dialysis and cancer patients and control group 25% , 30% , 22% respectively. As for the IgM antibody study did not record any positive result in both categories of the study (dialysis and cancer) only control group was 2%. The result of IgG & IgM together in renal dialysis and cancer and control group 3%, 6%, 0% respectively.The study found that the increase in the number of times the dialysis increased incidence of infection prevalence it was 25.53% for patients who conducted the dialysis treatment more than six times and 16.66% for patients who have less than six times.For factor chemical dosing show high infection rates in patients who have been exposed to more than one dose of a chemical 37.63% As for those who were not exposed to any chemical dose was ratios have 14.29% .


Article
Study of Epidemiology of Toxoplasmosis in Hemodialysis Patients in Baghdad Hospitals
دراسة في الوبائيه المصليه لداء المقوسات الكونديه لمرضى غسل الكلى في مستشفيات بغداد

Authors: Khawla H.Zghair خوله حوري زغير --- Ayser I. Abdul-Aziz أيسر عماد عبد العزيز
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2014 Volume: 55 Issue: 3B Pages: 1236-1242
Publisher: Baghdad University جامعة بغداد

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Abstract

This study aims to assess some seroprevalence of toxoplasmosis in chronic renal failure (CRF) patients undergoing hemodialysis in dialysis centers in some Baghdad hospitals, by testing the blood samples with enzyme linked Immunosorbant assay (ELISA) IgG and IgM, to determine the incidence of toxoplasmosis in hemodialysis patients. Hemodialysis patients infected with Toxoplasma. gondii were appeared with 129(32.25%) seropositive anti-Toxoplasma IgG. The age groups (43-51) year and (52-60) years of hemodialysis patients showed the highest percentage of anti-Toxoplasma gondii antibodies. An increase of the seropositivity rate was detected with increasing length of time on hemodialysis treatment. Females had the highest significant percentage of anti-Toxoplasma IgG 72 (55.81%) than males 57 (44.18%). Domestic poultry breeder had high percentage 71(55.03%) and 8 (57.14%) of anti-Toxoplasma (IgG+&IgM+) antibodies respectively in comparison with patients who do not breed domestic poultry.

تهدف هذه الدراسة لتقييم الانتشار المصلي لداء المقوسات الكوندية في مرضى CRF الذين يخضعون لغسل الكلى في مراكز غسل الكلى في مستشفيات بغداد. أستخدم اختبار الامتزاز المناعي المرتبط بالانزيم ((ELISA نوع IgG و IgM لتحديد نسبة حدوث داء المقوسات في مرضى غسل الكلى. أظهرت النتائج النسبة المئوية (32.25 %) 129 لمرضى غسل الكلى المصابين بطفيلي T. gondii والحاملين للضد المناعي IgG. أظهرت المجاميع العمرية (43-51) و(52-60) سنة نسبة الاصابة الاعلى بالاضداد المناعية المضادة للمقوسات. تم الكشف عن زيادة في معدل إيجابية المصل مع زيادة طول وقت العلاج بغسل الكلى. أظهرت الاناث اعلى نسبة اصابة مئوية بالاضداد المناعية IgG للمقوسات72 (55.81%) منها للذكور57 (44.18%). كما اظهر الاشخاص المربين للدواجن المحلية نسبة مئوية عالية للاصابة الموجبة بالاضداد المناعية IgG+ للمقوسات وللاصابة الموجبة لكلا الاضداد المناعية (IgG+ و +IgM) وكانت النسبة 71 (55.03%) و 8 (57.14%) على التوالي بالمقارنه مع الاشخاص غير المربين للدواجن.

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