research centers


Search results: Found 4

Listing 1 - 4 of 4
Sort by

Article
Immunohistochemical study of PDGF, IGF of radiated tooth rat embryo

Authors: Rasim Mahdi Salih راسم مهدي صالح --- Athraa Y Al-Hijazi عذراء يحيى الحجازي
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2013 Volume: 25 Issue: 1 Pages: 110-115
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

Background: Exposure to microwaves radiation from microwave oven may be harmful for users especially for the onewho have highest contact with microwave oven. Because the body is electrochemical in nature, any force thatdisrupts or changes human electrochemical events will affect the physiology of the body by destabilization andinterruption of many chemical body substance including growth factors.The insulin-like growth factors (IGFs) are afamily of mitogenic proteins that control growth, differentiation, and the maintenance of differentiated function innumerous tissues. It fulfils an important role in growth and development of teeth, mandible, maxillae, and tongue.Platelet derived growth factors (PDGF) are proteins that regulate cell growth and division. In particular, it plays asignificant role in blood vessel formation (angiogenesis). It seems that IGF and PDGF share in much tissuedevelopmental process. Therefore they included in the present study in correlation to tooth growth & development.This study Illustrates the expression of insulin like growth factor and platelet derived growth factor by dental cells ofrat embryos at periods of gestation 16th,18thday intrauterine life(I.U.L) and one day neonatal life.Materials and Methods: Animal model: Thirty-six female rats were used in this study .Starting from zero days (time ofgestation that recorded) the pregnant rats were divided into three groups. Group A serve as a control, groups Bexposed to microwave oven radiation For (15 /minutes; 5/min /hour for 3 hours continuously) daily and C exposed toEMF radiation for (45 minutes ;15 min /hour for3hours continuously)daily starting from zero day of gestation till the lastday. The embryo of rats at 16thday and 18th day of intrauterine life and one day old rat (new born rat) were studiedimmunohistochemically for localized of platelet derived growth factor (PDGF) and insulin growth factor (IGF) markers.Results:The results showed that experimental group (B) exposed to short duration of radiation (5/ min.) stimulates thedevelopment of tooth germ and faster tooth growing in comparison to control with immunohistochemical resultsshow strong to moderate intense stain for positive expression of growth factors(PDGF,IGF) by dental tissue.For longexposure period of radiation( group C) , it showed retardation in the tooth growth withimmunohistochemical findingsrecord weak to negative intense stain for the expression of growth factors(PDGF,IGF) by dental tissue.Conclusion: Exposure to microwave (oven) radiation during pregnancy may play a role in the expression of IGF andPDGF by cells of tooth germ thatinfluence on cell differentiation and physiological activity of specialized dental cells,depending on exposure time


Article
P57Kip2 immunostaining, a diagnostic marker in differentiating complete hydatidiform mole from its mimics
P57Kip2 المناعية ، علامة تشخيصية في التمييز بين الخلد المائي الكامل من مقلداته

Author: Ava T. Ismael
Journal: Zanco Journal of Medical Sciences مجلة زانكو للعلوم الطبية ISSN: 19955588/19955596 Year: 2016 Volume: 20 Issue: 2 Pages: 1316-1322
Publisher: Hawler Medical Univeristy جامعة هولير الطبية

Loading...
Loading...
Abstract

Background and objective: The distinction of hydatidiform mole from hydropic abortion remains a problem because of interobserver and intraobserver variability. This study aimed to determine the utility of p57Kip2 as a diagnostic marker in differentiating complete hydatidiform mole from its mimics.Methods: A total of 97 formalin fixed paraffin embedded material including forty cases of complete hydatidiform mole, 36 cases of partial hydatidiform mole and 21 cases of hydropic abortion were selected randomly from the files of histopathology laboratory of Maternity Teaching Hospital in Erbil. The samples were reviewed by two pathologists, afterward; immunohistochemical staining was performed by using a p57Kip2 marker. We considered p57Kip2 positive only if nuclear p57Kip2 staining was identified in at least 10% or more of all in a tissue section. Results: Negative immunostaining was seen in 77.5% of the complete hydatidiform mole in both villous cytotrophoblast and stromal cells. In contrast, 86.1% of partial hydatidiform mole showed positive immunostaining for p57Kip2. All cases of hydropic abortion 100% were positive for p57Kip2 immunostaining. In all gestations, p57Kip2 was strongly expressed in decidua which served as internal positive control. The concordance between the initial histological diagnosis and p57Kip2 immunostaining was statistically significant (P <0.001). Conclusions: p57Kip2 immunostaining is a highly sensitive and specific marker for diagnosis and classification of hydatidiform mole. p57Kip2 staining has the advantage of differentiating hydropic abortuses from the complete hydatidiform mole.


Article
Enhancement of tooth eruption by using amniotic stem cells (Immunohistochemical study of VEGF marker)

Authors: Athraa Y. Al- Hijazi عذراء الحجازي --- Lubna K. Jassim لبنى جاسم
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2013 Volume: 25 Issue: 2 Pages: 80-88
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

Background: Tooth eruption is a localized process in the jaws which exhibits precise timing and bilateral symmetry.Develop within the jaws and their eruption is a complex infancy process during which they move through bone totheir functional positions within the oral cavity. For species with more than one set of teeth, eruption of the second setalso accomplishes. The key to the successful clinical management of tooth eruption consists of understanding thatthis process consists largely of the local regulation of alveolar bone metabolism to produce bone resorption in thedirection of eruption and shift and formation of bone at the opposite side.The amniotic sac contains a considerablequantity of stem cells. These amniotic stem cells are able to differentiate into various tissues, which used in many field.Vascular endothelial growth factor (VEGF) is an important angiogenic factor reported to induce migration andproliferation of endothelial cells, enhance vascular permeability, and modulate thrombogenicity. VEGF expression incultured cells (smooth muscle cells, macrophages, endothelial cells) is controlled by growth factors and cytokines.The aim of this study was to study the administration of cell molecules of (Chorion, Amnion and Amniotic fluid)around developing mouse tooth and studying the expression of VEGF marker.Materials and Methods: forty eight albino Swiss mice of one day old age injected with isolated amniotic stem cells inthe anterior region of maxilla (incisors area) other 16 mice injected with saline represents control. Sacrifice 4 mice foreach period (4, 7, 10, and 13) day old age. The result were studied histologically and immunohistochemistry.Results: VEGF marker localized and identified in 3 areas; pulp, P.D.L, and Bone. In pulp. The mean value of positiveVEGF expression showed to be highest in Amnion group in comparison to the other studied groups. The marginalmean value of all periods reported to be highest in Amnion groups followed by Chorion group. The period 10 dayshowed highest marginal means value for positive VEGF expression for all groups. In P.D.L. area Amniotic fluid recordsthe highest mean and marginal mean value specifically at day-10 in comparison to other studied groups. In Bonearea Amniotic fluid records the highest mean and marginal mean value among the studied groups followed byChorion group. Period 7-day and 10-day shows high mean value for VEGF expression. Coincidence test for VEGFmarker illustrates to be affected by Amniotic fluid application in P.D.L. and in bone area while Amnion and Chorionapplication showed to be concerned with pulp.Conclusion. It reported that amniotic fluid application affected on expression of VEGF in P.D.L and bone whileamnion and chorion showed to affect on expression of VEGF in pulp.The present study highlighted on clinical andresearcher application of Amniotic fluid and Chorion for supplement of stem cell in dental tissue engineering or evenin other body tissues.


Article
Ki-67(MIB-1) and Progesterone Receptor in Meningioma An Immunohistochemical Study

Author: Wahda Mohammed Taib Al- Nuaimy
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2012 Volume: 11 Issue: 2 Pages: 157-167
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

Loading...
Loading...
Abstract

ABSTRACT:BACKGROUND: In meningioma the tumor grade, subtype, and extent of surgical resection are strong prognostic factors, the growth of this tumor is still unpredictable, and additional prognostic markers are needed. Many studies have shown that the detection of the proliferative potential of meningioma by Ki-67 (MIB-1) and the detection of the progesterone receptor might predict the natural history of tumor and patient survival. OBJECTIVE: To evaluate the proliferative fraction Ki-67 (MIB-1) and progesterone receptor in meningioma, To correlate the expression of these two markers with various clinico- pathological parameters, To compare these results with other studies . PATIENTS AND METHODS: This is a prospective study, in which 50 cases of meningioma were collected in Nineveh province. These cases were examined immunohistochemically. The results of these two markers were correlated with various clinico- pathological parameters. RESULTS: The mean age of all cases was 46 years. There were 16 male and 34 female patients. According to the W.H.O 2000 classification, there were 84% grade I, 10% were grade II and 6 %were grade III. Only 14% of the patients had a history of recurrence. Progesterone receptor was positive in 72% of all cases. Significant relation was observed between the progesterone receptor and the patients age .It was significantly higher in female than male. A significant relationship was found between progesterone receptor with grade and histological types. Thirty three out of 43 cases without recurrence had positive progesterone receptor. The mean±Standard deviation (SD) of Ki-67 Labeling Index (Ki-67 LI) was 2.7±4.2%. No significant relation was found between the Ki-67 LI and patients age. The male patients had a higher Ki-67 LI than that of the female. Significant relation was found between Ki-67 LI and the grade of meningioma . The mean of Ki-67 LI in the recurrent cases was higher than in non recurrent cases. An inverse relationship was found between Ki-67 LI and progesterone receptor . CONCLUSION: Progesterone receptor showed a significant relation with the age and histological types. Ki-67 LI had a direct significant association with the grade and the recurrent cases., while inverse relation was observed between the progesterone receptor with the grade and the recurrent cases. An inverse relationship was observed between the progesterone receptor and Ki-67 LI.

Listing 1 - 4 of 4
Sort by
Narrow your search

Resource type

article (4)


Language

English (4)


Year
From To Submit

2016 (1)

2013 (2)

2012 (1)